Ignavigranum ruoffiae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Aerococcaceae; Ignavigranum

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1874 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9B3C8|A0A1H9B3C8_9LACT Uncharacterized protein OS=Ignavigranum ruoffiae OX=89093 GN=SAMN04488558_102151 PE=4 SV=1
MM1 pKa = 7.96DD2 pKa = 5.81AYY4 pKa = 9.49FTYY7 pKa = 10.53PEE9 pKa = 4.47LVEE12 pKa = 4.12EE13 pKa = 5.16AYY15 pKa = 10.82SFYY18 pKa = 10.85QNSDD22 pKa = 2.84IEE24 pKa = 4.2IDD26 pKa = 4.74KK27 pKa = 11.18LSLWRR32 pKa = 11.84NMVDD36 pKa = 4.96LGILDD41 pKa = 4.59GLGQPTSAAINSGLVRR57 pKa = 11.84EE58 pKa = 5.17FIEE61 pKa = 4.75EE62 pKa = 4.17EE63 pKa = 4.05NLSLAEE69 pKa = 4.11FKK71 pKa = 10.48EE72 pKa = 4.46VYY74 pKa = 9.83PVFDD78 pKa = 4.32RR79 pKa = 11.84YY80 pKa = 10.92SDD82 pKa = 3.4QFFIFQDD89 pKa = 3.96GFWQVHH95 pKa = 6.34ADD97 pKa = 4.25LLDD100 pKa = 5.05LIQIDD105 pKa = 4.2IEE107 pKa = 4.72DD108 pKa = 4.41GSLSAPEE115 pKa = 4.15VMEE118 pKa = 4.7LEE120 pKa = 5.03AYY122 pKa = 9.96FNNQIDD128 pKa = 5.68DD129 pKa = 3.71IFKK132 pKa = 10.71DD133 pKa = 3.46

Molecular weight: 15.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H9C3G4|A0A1H9C3G4_9LACT 50S ribosomal protein L7/L12 OS=Ignavigranum ruoffiae OX=89093 GN=rplL PE=3 SV=1
MM1 pKa = 6.99AQRR4 pKa = 11.84RR5 pKa = 11.84GGRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84KK12 pKa = 9.16VCYY15 pKa = 9.78FCANHH20 pKa = 6.94IDD22 pKa = 3.6HH23 pKa = 7.85PDD25 pKa = 3.55YY26 pKa = 11.37KK27 pKa = 10.85DD28 pKa = 3.48VEE30 pKa = 4.21LLGRR34 pKa = 11.84FVSDD38 pKa = 3.33KK39 pKa = 11.36GKK41 pKa = 9.83ILPRR45 pKa = 11.84RR46 pKa = 11.84VTGTCAKK53 pKa = 9.56HH54 pKa = 5.56QRR56 pKa = 11.84ALTTAIKK63 pKa = 10.16RR64 pKa = 11.84ARR66 pKa = 11.84IMALLPFTVQDD77 pKa = 3.47

Molecular weight: 8.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1874

0

1874

577246

32

1435

308.0

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.34 ± 0.056

0.597 ± 0.015

5.72 ± 0.05

6.884 ± 0.065

4.314 ± 0.04

6.233 ± 0.058

2.077 ± 0.024

8.046 ± 0.063

5.784 ± 0.047

10.38 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.653 ± 0.023

4.523 ± 0.038

3.531 ± 0.031

5.623 ± 0.062

4.074 ± 0.039

6.02 ± 0.053

4.948 ± 0.038

6.374 ± 0.043

0.939 ± 0.022

3.939 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski