Fusarium poae victorivirus 1
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B4ZA57|A0A1B4ZA57_9VIRU RNA-directed RNA polymerase OS=Fusarium poae victorivirus 1 OX=1849535 PE=3 SV=1
MM1 pKa = 7.66 PLSSPVFSSPAGRR14 pKa = 11.84 VPLIRR19 pKa = 11.84 PIEE22 pKa = 4.05 LKK24 pKa = 10.76 RR25 pKa = 11.84 PINALFYY32 pKa = 10.97 NSGAMAQPQSFRR44 pKa = 11.84 TATTSALTGAVAGVSSGQIQDD65 pKa = 3.57 DD66 pKa = 3.63 NTYY69 pKa = 9.99 RR70 pKa = 11.84 RR71 pKa = 11.84 YY72 pKa = 10.22 RR73 pKa = 11.84 SGLSIGVHH81 pKa = 4.39 EE82 pKa = 5.24 HH83 pKa = 4.0 GHH85 pKa = 4.86 YY86 pKa = 10.11 AYY88 pKa = 9.7 KK89 pKa = 10.29 RR90 pKa = 11.84 SSVFYY95 pKa = 9.97 EE96 pKa = 3.63 VGRR99 pKa = 11.84 RR100 pKa = 11.84 YY101 pKa = 10.48 ARR103 pKa = 11.84 LTQALAGRR111 pKa = 11.84 PEE113 pKa = 4.18 GGRR116 pKa = 11.84 AGFDD120 pKa = 2.94 ASVMVNPAEE129 pKa = 4.13 AANFEE134 pKa = 3.9 GWARR138 pKa = 11.84 RR139 pKa = 11.84 FSNFSPAWDD148 pKa = 3.65 MMDD151 pKa = 3.23 LAGVVEE157 pKa = 4.77 RR158 pKa = 11.84 LAKK161 pKa = 10.18 AVAAQSVYY169 pKa = 11.18 GGVSTTNMRR178 pKa = 11.84 AGFPVSVVALGTLDD192 pKa = 3.79 SPQTASSSSVFIPRR206 pKa = 11.84 TVDD209 pKa = 3.13 RR210 pKa = 11.84 VGNDD214 pKa = 2.73 NVFAVLAAAANGEE227 pKa = 4.38 GATVTTDD234 pKa = 3.42 VVRR237 pKa = 11.84 LDD239 pKa = 3.42 AATNQPVVPAVQGQALAQAAVEE261 pKa = 4.12 GLRR264 pKa = 11.84 ILGANFEE271 pKa = 4.35 RR272 pKa = 11.84 SGAGDD277 pKa = 3.57 IFAYY281 pKa = 10.49 ALTRR285 pKa = 11.84 GIHH288 pKa = 5.32 SVVSVVSHH296 pKa = 5.9 TDD298 pKa = 2.71 EE299 pKa = 5.02 GGWLRR304 pKa = 11.84 GVLRR308 pKa = 11.84 RR309 pKa = 11.84 TGFRR313 pKa = 11.84 APYY316 pKa = 10.29 GGINQDD322 pKa = 3.4 LRR324 pKa = 11.84 DD325 pKa = 4.04 YY326 pKa = 10.64 PQLPPLASLSTAATSAWVDD345 pKa = 4.32 AIALKK350 pKa = 9.23 TAAIVAHH357 pKa = 6.98 CDD359 pKa = 2.99 PCVRR363 pKa = 11.84 ADD365 pKa = 3.18 GGYY368 pKa = 8.88 YY369 pKa = 9.98 PSVFTASSGDD379 pKa = 3.22 ISPPGTEE386 pKa = 3.71 EE387 pKa = 4.72 GEE389 pKa = 4.31 VPEE392 pKa = 5.23 AVATNIGRR400 pKa = 11.84 QISSDD405 pKa = 3.31 VGRR408 pKa = 11.84 FAPLYY413 pKa = 9.82 MRR415 pKa = 11.84 GLCYY419 pKa = 10.46 LFGMSSTSGIAEE431 pKa = 3.97 AHH433 pKa = 6.27 FSTTAADD440 pKa = 3.89 HH441 pKa = 6.98 LSGTTDD447 pKa = 2.29 RR448 pKa = 11.84 HH449 pKa = 5.06 LRR451 pKa = 11.84 HH452 pKa = 5.77 RR453 pKa = 11.84 TVAPYY458 pKa = 9.74 FWIEE462 pKa = 3.95 PTSLVPTNALGTTAEE477 pKa = 4.15 AAGFGALTTAGVEE490 pKa = 3.88 AAIPAFEE497 pKa = 4.11 RR498 pKa = 11.84 VRR500 pKa = 11.84 EE501 pKa = 3.95 IDD503 pKa = 3.22 HH504 pKa = 5.95 GHH506 pKa = 5.99 NANFTTIGFKK516 pKa = 9.48 MRR518 pKa = 11.84 SARR521 pKa = 11.84 TSGLVAAYY529 pKa = 8.95 AANPAEE535 pKa = 4.14 LSGLRR540 pKa = 11.84 LYY542 pKa = 10.82 QFDD545 pKa = 3.8 EE546 pKa = 4.81 SSVVLAGNNGPTNGDD561 pKa = 3.03 VPAKK565 pKa = 10.46 HH566 pKa = 6.1 NAADD570 pKa = 4.06 PLSSYY575 pKa = 10.7 LWTRR579 pKa = 11.84 GQSAIPAPAEE589 pKa = 3.65 FMNIQGSYY597 pKa = 7.41 AAKK600 pKa = 10.2 YY601 pKa = 10.07 KK602 pKa = 10.6 VVDD605 pKa = 3.58 WDD607 pKa = 3.99 DD608 pKa = 4.25 DD609 pKa = 4.56 FNGTLGPLPEE619 pKa = 4.11 AWEE622 pKa = 4.48 LEE624 pKa = 4.18 SHH626 pKa = 5.05 PTKK629 pKa = 10.26 WRR631 pKa = 11.84 TSVPTALPAGASNYY645 pKa = 9.73 ADD647 pKa = 3.04 SGARR651 pKa = 11.84 RR652 pKa = 11.84 ARR654 pKa = 11.84 SRR656 pKa = 11.84 AGVALAQATLRR667 pKa = 11.84 NRR669 pKa = 11.84 GLGDD673 pKa = 3.54 ANSPVISVSNVPPSWDD689 pKa = 3.56 DD690 pKa = 3.28 EE691 pKa = 4.61 RR692 pKa = 11.84 PATTRR697 pKa = 11.84 LDD699 pKa = 3.55 DD700 pKa = 3.52 TRR702 pKa = 11.84 TAEE705 pKa = 4.23 HH706 pKa = 6.56 NPAPGVVVTPGQGTDD721 pKa = 3.25 TADD724 pKa = 3.72 TPAHH728 pKa = 6.42 LAAPALPIPHH738 pKa = 5.72 QQPLRR743 pKa = 11.84 GAPYY747 pKa = 9.35 PPRR750 pKa = 11.84 APGQLGGAQPPPPPVGPPAPPGPPGGPPNDD780 pKa = 5.42 DD781 pKa = 4.84 DD782 pKa = 4.56 NQGPPPQAPPADD794 pKa = 3.8 NHH796 pKa = 6.15 PVPPAAA802 pKa = 5.21
Molecular weight: 83.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.915
IPC2_protein 5.792
IPC_protein 5.868
Toseland 6.376
ProMoST 6.237
Dawson 6.16
Bjellqvist 6.148
Wikipedia 6.173
Rodwell 6.148
Grimsley 6.59
Solomon 6.16
Lehninger 6.148
Nozaki 6.427
DTASelect 6.634
Thurlkill 6.649
EMBOSS 6.605
Sillero 6.532
Patrickios 4.24
IPC_peptide 6.173
IPC2_peptide 6.547
IPC2.peptide.svr19 6.436
Protein with the highest isoelectric point:
>tr|A0A1B4ZA57|A0A1B4ZA57_9VIRU RNA-directed RNA polymerase OS=Fusarium poae victorivirus 1 OX=1849535 PE=3 SV=1
MM1 pKa = 7.41 SPIDD5 pKa = 4.01 RR6 pKa = 11.84 GDD8 pKa = 3.55 GEE10 pKa = 4.6 AFLDD14 pKa = 4.31 GVLDD18 pKa = 4.38 SILKK22 pKa = 9.94 RR23 pKa = 11.84 FPGQLPDD30 pKa = 3.54 VAGLPFDD37 pKa = 4.1 EE38 pKa = 5.26 QISAAYY44 pKa = 8.81 SPSWGGVRR52 pKa = 11.84 PSGLLRR58 pKa = 11.84 AAFSYY63 pKa = 10.6 RR64 pKa = 11.84 HH65 pKa = 4.79 TTVPVQSIYY74 pKa = 11.16 DD75 pKa = 3.59 HH76 pKa = 6.83 SDD78 pKa = 2.59 LRR80 pKa = 11.84 RR81 pKa = 11.84 LLNSVVEE88 pKa = 4.84 FFPLSTDD95 pKa = 4.15 FSSFKK100 pKa = 10.32 ILKK103 pKa = 9.45 HH104 pKa = 5.82 PKK106 pKa = 9.85 NSLKK110 pKa = 10.58 LFKK113 pKa = 10.23 PKK115 pKa = 10.35 RR116 pKa = 11.84 LPQALTKK123 pKa = 10.9 ANLYY127 pKa = 10.08 LDD129 pKa = 3.58 EE130 pKa = 4.55 VLRR133 pKa = 11.84 DD134 pKa = 3.54 LCKK137 pKa = 10.53 NRR139 pKa = 11.84 PTLGEE144 pKa = 3.99 EE145 pKa = 3.97 ASALLWSLRR154 pKa = 11.84 DD155 pKa = 3.37 RR156 pKa = 11.84 GITHH160 pKa = 7.6 DD161 pKa = 3.69 AATAIVLYY169 pKa = 10.58 GSALSQFYY177 pKa = 10.59 TDD179 pKa = 3.59 AFHH182 pKa = 6.83 WAATAVLYY190 pKa = 9.26 PKK192 pKa = 10.01 LAKK195 pKa = 10.2 AVSNFLKK202 pKa = 10.22 ATGGNATSFGSLLVEE217 pKa = 4.43 CEE219 pKa = 4.14 VLQGRR224 pKa = 11.84 GVGTIDD230 pKa = 4.35 LLAEE234 pKa = 4.3 AKK236 pKa = 10.09 LRR238 pKa = 11.84 CSPDD242 pKa = 3.29 YY243 pKa = 11.46 VRR245 pKa = 11.84 DD246 pKa = 3.6 HH247 pKa = 6.29 YY248 pKa = 11.51 AAAFDD253 pKa = 3.89 EE254 pKa = 4.71 EE255 pKa = 4.41 KK256 pKa = 10.7 LRR258 pKa = 11.84 RR259 pKa = 11.84 AVRR262 pKa = 11.84 RR263 pKa = 11.84 VYY265 pKa = 8.98 EE266 pKa = 4.13 TEE268 pKa = 3.77 ISHH271 pKa = 6.85 EE272 pKa = 4.4 DD273 pKa = 3.16 DD274 pKa = 3.43 SQRR277 pKa = 11.84 VEE279 pKa = 4.3 FPTLEE284 pKa = 4.17 EE285 pKa = 4.15 HH286 pKa = 7.09 WDD288 pKa = 3.86 SRR290 pKa = 11.84 WVWAVNGSQSSLLDD304 pKa = 3.3 GGRR307 pKa = 11.84 VKK309 pKa = 10.7 KK310 pKa = 10.59 LLEE313 pKa = 3.93 PLGLHH318 pKa = 6.14 KK319 pKa = 10.42 LHH321 pKa = 6.54 RR322 pKa = 11.84 RR323 pKa = 11.84 AWLEE327 pKa = 3.88 CTPDD331 pKa = 3.69 DD332 pKa = 5.34 PRR334 pKa = 11.84 VGWDD338 pKa = 2.85 GTTYY342 pKa = 10.71 VSPSPKK348 pKa = 10.18 LEE350 pKa = 4.09 NGKK353 pKa = 7.79 TRR355 pKa = 11.84 AIFACDD361 pKa = 3.12 TRR363 pKa = 11.84 HH364 pKa = 5.45 YY365 pKa = 10.91 LAFEE369 pKa = 4.27 HH370 pKa = 7.0 LLTPVEE376 pKa = 4.13 KK377 pKa = 9.41 RR378 pKa = 11.84 WRR380 pKa = 11.84 GSRR383 pKa = 11.84 VVLNPGKK390 pKa = 10.53 GGNIAMAQRR399 pKa = 11.84 VRR401 pKa = 11.84 HH402 pKa = 5.45 SRR404 pKa = 11.84 DD405 pKa = 2.85 RR406 pKa = 11.84 SGISLMLDD414 pKa = 2.95 YY415 pKa = 11.45 DD416 pKa = 4.37 DD417 pKa = 6.41 FNSQHH422 pKa = 5.4 TTSAMKK428 pKa = 10.09 IVIEE432 pKa = 4.15 EE433 pKa = 4.25 LCSVVGYY440 pKa = 8.94 PADD443 pKa = 3.62 LTATLVSSFDD453 pKa = 3.04 KK454 pKa = 10.63 MRR456 pKa = 11.84 IHH458 pKa = 6.91 VAGKK462 pKa = 9.61 YY463 pKa = 9.83 VGVAAGTLMSGHH475 pKa = 7.16 RR476 pKa = 11.84 ATTFINSVLNKK487 pKa = 10.25 AYY489 pKa = 10.65 LDD491 pKa = 3.82 VVLGEE496 pKa = 4.26 GWLDD500 pKa = 3.36 TRR502 pKa = 11.84 RR503 pKa = 11.84 SVHH506 pKa = 6.49 VGDD509 pKa = 6.07 DD510 pKa = 3.7 IYY512 pKa = 11.39 CGVKK516 pKa = 9.99 SYY518 pKa = 11.27 RR519 pKa = 11.84 DD520 pKa = 3.18 AAYY523 pKa = 9.91 VVHH526 pKa = 6.76 QITSSPLRR534 pKa = 11.84 MNPTKK539 pKa = 10.55 QSVGHH544 pKa = 6.08 VSTEE548 pKa = 3.8 FLRR551 pKa = 11.84 LATAGRR557 pKa = 11.84 DD558 pKa = 3.29 TYY560 pKa = 11.38 GYY562 pKa = 8.51 VARR565 pKa = 11.84 SIASLISGNWVSDD578 pKa = 4.02 RR579 pKa = 11.84 IMNSYY584 pKa = 9.7 EE585 pKa = 3.8 ALTTMVASARR595 pKa = 11.84 TLANRR600 pKa = 11.84 ARR602 pKa = 11.84 DD603 pKa = 3.59 VTLPLLLEE611 pKa = 4.36 SAVKK615 pKa = 10.23 RR616 pKa = 11.84 VLSKK620 pKa = 11.2 DD621 pKa = 3.88 CIDD624 pKa = 3.91 DD625 pKa = 3.63 RR626 pKa = 11.84 VIRR629 pKa = 11.84 RR630 pKa = 11.84 LLLGEE635 pKa = 3.94 VAINNGPTFSSSGSYY650 pKa = 9.44 TRR652 pKa = 11.84 VTVRR656 pKa = 11.84 AEE658 pKa = 3.57 YY659 pKa = 8.92 TARR662 pKa = 11.84 DD663 pKa = 3.49 TVGRR667 pKa = 11.84 PKK669 pKa = 10.53 LPHH672 pKa = 6.15 QSTSRR677 pKa = 11.84 YY678 pKa = 7.74 LTTNTTDD685 pKa = 4.56 FEE687 pKa = 5.03 CEE689 pKa = 4.0 TLSQAGISPEE699 pKa = 3.69 QTMIEE704 pKa = 4.09 SSYY707 pKa = 11.39 SKK709 pKa = 10.64 SLQFGDD715 pKa = 4.72 LYY717 pKa = 11.05 FDD719 pKa = 3.76 RR720 pKa = 11.84 LVAGDD725 pKa = 4.07 VEE727 pKa = 4.73 STPARR732 pKa = 11.84 GSVGVEE738 pKa = 3.66 WLVHH742 pKa = 4.44 TTPPKK747 pKa = 10.64 GVLSQYY753 pKa = 10.58 PLLVLVKK760 pKa = 10.35 SRR762 pKa = 11.84 LPEE765 pKa = 3.93 YY766 pKa = 10.38 VVRR769 pKa = 11.84 DD770 pKa = 3.63 AVRR773 pKa = 11.84 RR774 pKa = 11.84 AGGNPQCTDD783 pKa = 3.72 LEE785 pKa = 4.36 LEE787 pKa = 3.9 AWGEE791 pKa = 4.0 YY792 pKa = 9.17 SHH794 pKa = 6.64 GCIVNDD800 pKa = 3.16 VLAYY804 pKa = 10.49 SDD806 pKa = 3.53 AAAFGKK812 pKa = 8.92 RR813 pKa = 11.84 TDD815 pKa = 3.81 CSVLTSTHH823 pKa = 6.48 RR824 pKa = 11.84 YY825 pKa = 8.0 YY826 pKa = 11.38 VV827 pKa = 3.18
Molecular weight: 91.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.127
IPC2_protein 7.512
IPC_protein 7.366
Toseland 7.161
ProMoST 7.936
Dawson 8.053
Bjellqvist 8.404
Wikipedia 7.98
Rodwell 8.068
Grimsley 7.249
Solomon 8.112
Lehninger 8.126
Nozaki 8.536
DTASelect 8.185
Thurlkill 8.229
EMBOSS 8.273
Sillero 8.521
Patrickios 4.329
IPC_peptide 8.097
IPC2_peptide 7.483
IPC2.peptide.svr19 7.472
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1629
802
827
814.5
87.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.357 ± 2.279
0.921 ± 0.385
5.77 ± 0.464
4.236 ± 0.437
3.192 ± 0.035
8.226 ± 0.97
2.517 ± 0.192
3.131 ± 0.097
2.64 ± 1.07
8.717 ± 1.575
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.228 ± 0.013
3.315 ± 0.476
6.937 ± 1.878
2.64 ± 0.337
7.182 ± 0.405
8.349 ± 0.524
6.753 ± 0.014
8.042 ± 0.307
1.412 ± 0.116
3.438 ± 0.402
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here