Faustovirus
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 492 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0H3TNA4|A0A0H3TNA4_9VIRU Uncharacterized protein OS=Faustovirus OX=1477405 GN=D5a_00432 PE=4 SV=1
MM1 pKa = 7.53 EE2 pKa = 5.16 GCLVLKK8 pKa = 10.23 IDD10 pKa = 5.09 ASEE13 pKa = 4.6 LPNDD17 pKa = 3.81 TQISYY22 pKa = 11.35 KK23 pKa = 10.31 DD24 pKa = 3.66 GKK26 pKa = 10.45 IKK28 pKa = 10.94 LSDD31 pKa = 3.51 SVGNIVNFEE40 pKa = 4.31 TNEE43 pKa = 3.76 LTILHH48 pKa = 6.49 SKK50 pKa = 8.06 TVVVNSCAYY59 pKa = 9.82 IITAPNVDD67 pKa = 3.42 NLRR70 pKa = 11.84 GFRR73 pKa = 11.84 YY74 pKa = 9.8 YY75 pKa = 11.07 VVDD78 pKa = 3.34 NEE80 pKa = 3.98 IRR82 pKa = 11.84 KK83 pKa = 8.6 EE84 pKa = 3.88 QLEE87 pKa = 4.13 NDD89 pKa = 4.5 DD90 pKa = 5.85 NDD92 pKa = 6.14 DD93 pKa = 4.72 NDD95 pKa = 3.86 NDD97 pKa = 3.97 NNKK100 pKa = 10.09 DD101 pKa = 3.51 NCSNCSDD108 pKa = 4.84 DD109 pKa = 6.64 CDD111 pKa = 5.7 DD112 pKa = 6.56 CDD114 pKa = 4.45 DD115 pKa = 5.17 CNNCEE120 pKa = 4.17 CDD122 pKa = 4.02 SNCEE126 pKa = 4.02 CEE128 pKa = 4.2 EE129 pKa = 4.01
Molecular weight: 14.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.769
IPC2_protein 3.783
IPC_protein 3.783
Toseland 3.567
ProMoST 3.935
Dawson 3.783
Bjellqvist 3.973
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.465
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.151
Thurlkill 3.617
EMBOSS 3.732
Sillero 3.91
Patrickios 0.477
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.831
Protein with the highest isoelectric point:
>tr|A0A0H3TPB6|A0A0H3TPB6_9VIRU Uncharacterized protein OS=Faustovirus OX=1477405 GN=E23_00304 PE=4 SV=1
MM1 pKa = 6.41 TTLLIIHH8 pKa = 7.43 RR9 pKa = 11.84 YY10 pKa = 10.1 GEE12 pKa = 4.25 LCGALQGRR20 pKa = 11.84 RR21 pKa = 11.84 PRR23 pKa = 11.84 KK24 pKa = 6.61 TRR26 pKa = 11.84 KK27 pKa = 9.44 GRR29 pKa = 11.84 LFAQGRR35 pKa = 11.84 SGAKK39 pKa = 9.68 PGANTLSDD47 pKa = 4.39 DD48 pKa = 3.73 FTSNLRR54 pKa = 11.84 DD55 pKa = 3.47 NDD57 pKa = 3.68 ILSQEE62 pKa = 4.0
Molecular weight: 6.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.458
IPC_protein 10.131
Toseland 10.613
ProMoST 10.262
Dawson 10.701
Bjellqvist 10.394
Wikipedia 10.891
Rodwell 10.877
Grimsley 10.73
Solomon 10.833
Lehninger 10.804
Nozaki 10.613
DTASelect 10.379
Thurlkill 10.599
EMBOSS 11.008
Sillero 10.628
Patrickios 10.716
IPC_peptide 10.833
IPC2_peptide 9.414
IPC2.peptide.svr19 8.619
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
492
0
492
135304
32
2979
275.0
31.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.124 ± 0.166
1.973 ± 0.082
6.134 ± 0.089
5.378 ± 0.105
3.691 ± 0.064
5.346 ± 0.115
2.205 ± 0.065
8.294 ± 0.11
7.004 ± 0.123
8.157 ± 0.102
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.6 ± 0.054
6.738 ± 0.1
3.63 ± 0.081
2.831 ± 0.058
4.644 ± 0.081
6.199 ± 0.096
6.341 ± 0.124
6.528 ± 0.089
1.061 ± 0.044
5.12 ± 0.104
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here