Amino acid dipepetide frequency for Paenibacillus phage Unity

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.124AlaAla: 8.124 ± 0.67
1.023AlaCys: 1.023 ± 0.204
5.821AlaAsp: 5.821 ± 0.567
6.844AlaGlu: 6.844 ± 0.721
2.942AlaPhe: 2.942 ± 0.384
4.862AlaGly: 4.862 ± 0.737
1.215AlaHis: 1.215 ± 0.214
4.286AlaIle: 4.286 ± 0.455
6.077AlaLys: 6.077 ± 0.576
7.612AlaLeu: 7.612 ± 0.652
1.791AlaMet: 1.791 ± 0.306
3.582AlaAsn: 3.582 ± 0.523
2.431AlaPro: 2.431 ± 0.348
2.047AlaGln: 2.047 ± 0.348
4.414AlaArg: 4.414 ± 0.474
4.35AlaSer: 4.35 ± 0.429
3.774AlaThr: 3.774 ± 0.524
6.333AlaVal: 6.333 ± 0.679
1.343AlaTrp: 1.343 ± 0.36
3.134AlaTyr: 3.134 ± 0.491
0.0AlaXaa: 0.0 ± 0.0
Cys
0.896CysAla: 0.896 ± 0.236
0.064CysCys: 0.064 ± 0.065
0.448CysAsp: 0.448 ± 0.184
0.448CysGlu: 0.448 ± 0.163
0.512CysPhe: 0.512 ± 0.166
0.96CysGly: 0.96 ± 0.232
0.256CysHis: 0.256 ± 0.131
0.448CysIle: 0.448 ± 0.162
0.64CysLys: 0.64 ± 0.178
0.768CysLeu: 0.768 ± 0.232
0.256CysMet: 0.256 ± 0.116
0.192CysAsn: 0.192 ± 0.102
0.512CysPro: 0.512 ± 0.16
0.256CysGln: 0.256 ± 0.108
1.087CysArg: 1.087 ± 0.264
0.704CysSer: 0.704 ± 0.287
0.384CysThr: 0.384 ± 0.16
0.448CysVal: 0.448 ± 0.166
0.192CysTrp: 0.192 ± 0.097
0.32CysTyr: 0.32 ± 0.138
0.0CysXaa: 0.0 ± 0.0
Asp
4.989AspAla: 4.989 ± 0.672
0.448AspCys: 0.448 ± 0.155
5.821AspAsp: 5.821 ± 0.529
6.269AspGlu: 6.269 ± 0.52
3.454AspPhe: 3.454 ± 0.418
4.35AspGly: 4.35 ± 0.531
1.343AspHis: 1.343 ± 0.246
4.989AspIle: 4.989 ± 0.539
2.751AspLys: 2.751 ± 0.393
4.989AspLeu: 4.989 ± 0.492
1.919AspMet: 1.919 ± 0.3
2.175AspAsn: 2.175 ± 0.34
2.303AspPro: 2.303 ± 0.395
1.535AspGln: 1.535 ± 0.336
3.646AspArg: 3.646 ± 0.392
3.838AspSer: 3.838 ± 0.379
3.454AspThr: 3.454 ± 0.45
5.501AspVal: 5.501 ± 0.568
1.599AspTrp: 1.599 ± 0.32
2.495AspTyr: 2.495 ± 0.391
0.0AspXaa: 0.0 ± 0.0
Glu
5.629GluAla: 5.629 ± 0.582
0.768GluCys: 0.768 ± 0.281
3.902GluAsp: 3.902 ± 0.478
6.972GluGlu: 6.972 ± 0.701
2.879GluPhe: 2.879 ± 0.425
4.158GluGly: 4.158 ± 0.525
1.215GluHis: 1.215 ± 0.296
4.67GluIle: 4.67 ± 0.536
6.077GluLys: 6.077 ± 0.599
8.444GluLeu: 8.444 ± 0.781
2.431GluMet: 2.431 ± 0.334
2.559GluAsn: 2.559 ± 0.328
2.879GluPro: 2.879 ± 0.417
2.815GluGln: 2.815 ± 0.405
4.989GluArg: 4.989 ± 0.539
3.774GluSer: 3.774 ± 0.482
4.286GluThr: 4.286 ± 0.449
5.181GluVal: 5.181 ± 0.689
1.215GluTrp: 1.215 ± 0.252
3.774GluTyr: 3.774 ± 0.408
0.0GluXaa: 0.0 ± 0.0
Phe
3.198PheAla: 3.198 ± 0.501
0.256PheCys: 0.256 ± 0.123
2.687PheAsp: 2.687 ± 0.388
2.431PheGlu: 2.431 ± 0.497
1.215PhePhe: 1.215 ± 0.313
2.559PheGly: 2.559 ± 0.391
0.64PheHis: 0.64 ± 0.221
2.239PheIle: 2.239 ± 0.382
2.367PheLys: 2.367 ± 0.289
2.431PheLeu: 2.431 ± 0.431
1.087PheMet: 1.087 ± 0.3
1.855PheAsn: 1.855 ± 0.381
1.151PhePro: 1.151 ± 0.305
0.704PheGln: 0.704 ± 0.174
2.239PheArg: 2.239 ± 0.351
1.855PheSer: 1.855 ± 0.404
2.303PheThr: 2.303 ± 0.322
2.751PheVal: 2.751 ± 0.369
0.256PheTrp: 0.256 ± 0.106
1.599PheTyr: 1.599 ± 0.313
0.0PheXaa: 0.0 ± 0.0
Gly
3.966GlyAla: 3.966 ± 0.621
0.576GlyCys: 0.576 ± 0.18
4.542GlyAsp: 4.542 ± 0.624
4.286GlyGlu: 4.286 ± 0.655
2.239GlyPhe: 2.239 ± 0.333
5.309GlyGly: 5.309 ± 0.683
1.151GlyHis: 1.151 ± 0.28
5.181GlyIle: 5.181 ± 0.584
6.077GlyLys: 6.077 ± 0.561
5.693GlyLeu: 5.693 ± 0.652
1.663GlyMet: 1.663 ± 0.366
2.623GlyAsn: 2.623 ± 0.38
1.791GlyPro: 1.791 ± 0.348
1.919GlyGln: 1.919 ± 0.367
4.67GlyArg: 4.67 ± 0.582
2.431GlySer: 2.431 ± 0.372
3.838GlyThr: 3.838 ± 0.434
4.798GlyVal: 4.798 ± 0.568
1.215GlyTrp: 1.215 ± 0.266
2.495GlyTyr: 2.495 ± 0.409
0.0GlyXaa: 0.0 ± 0.0
His
1.855HisAla: 1.855 ± 0.401
0.192HisCys: 0.192 ± 0.105
1.535HisAsp: 1.535 ± 0.327
1.727HisGlu: 1.727 ± 0.332
0.64HisPhe: 0.64 ± 0.177
1.279HisGly: 1.279 ± 0.279
0.384HisHis: 0.384 ± 0.168
1.023HisIle: 1.023 ± 0.236
1.087HisLys: 1.087 ± 0.226
1.151HisLeu: 1.151 ± 0.34
0.32HisMet: 0.32 ± 0.117
0.576HisAsn: 0.576 ± 0.189
0.96HisPro: 0.96 ± 0.228
0.64HisGln: 0.64 ± 0.169
0.96HisArg: 0.96 ± 0.334
0.64HisSer: 0.64 ± 0.176
0.768HisThr: 0.768 ± 0.242
1.343HisVal: 1.343 ± 0.269
0.192HisTrp: 0.192 ± 0.099
1.087HisTyr: 1.087 ± 0.254
0.0HisXaa: 0.0 ± 0.0
Ile
5.693IleAla: 5.693 ± 0.63
1.087IleCys: 1.087 ± 0.315
5.629IleAsp: 5.629 ± 0.552
4.222IleGlu: 4.222 ± 0.367
1.599IlePhe: 1.599 ± 0.321
4.094IleGly: 4.094 ± 0.48
1.023IleHis: 1.023 ± 0.213
3.582IleIle: 3.582 ± 0.419
4.286IleLys: 4.286 ± 0.554
4.158IleLeu: 4.158 ± 0.511
1.599IleMet: 1.599 ± 0.355
2.175IleAsn: 2.175 ± 0.368
2.751IlePro: 2.751 ± 0.431
1.599IleGln: 1.599 ± 0.304
4.798IleArg: 4.798 ± 0.419
2.879IleSer: 2.879 ± 0.537
3.262IleThr: 3.262 ± 0.503
4.35IleVal: 4.35 ± 0.641
0.576IleTrp: 0.576 ± 0.183
2.431IleTyr: 2.431 ± 0.423
0.0IleXaa: 0.0 ± 0.0
Lys
5.693LysAla: 5.693 ± 0.533
0.768LysCys: 0.768 ± 0.232
4.542LysAsp: 4.542 ± 0.439
4.222LysGlu: 4.222 ± 0.597
1.919LysPhe: 1.919 ± 0.447
4.35LysGly: 4.35 ± 0.632
1.599LysHis: 1.599 ± 0.334
4.67LysIle: 4.67 ± 0.454
5.437LysLys: 5.437 ± 0.771
6.461LysLeu: 6.461 ± 0.801
1.343LysMet: 1.343 ± 0.305
2.175LysAsn: 2.175 ± 0.449
2.431LysPro: 2.431 ± 0.311
2.495LysGln: 2.495 ± 0.48
5.885LysArg: 5.885 ± 0.586
3.902LysSer: 3.902 ± 0.447
3.838LysThr: 3.838 ± 0.476
4.67LysVal: 4.67 ± 0.578
1.407LysTrp: 1.407 ± 0.341
3.134LysTyr: 3.134 ± 0.459
0.0LysXaa: 0.0 ± 0.0
Leu
7.868LeuAla: 7.868 ± 0.648
0.768LeuCys: 0.768 ± 0.239
6.141LeuAsp: 6.141 ± 0.609
5.565LeuGlu: 5.565 ± 0.515
2.751LeuPhe: 2.751 ± 0.387
4.734LeuGly: 4.734 ± 0.442
1.855LeuHis: 1.855 ± 0.382
4.734LeuIle: 4.734 ± 0.533
4.542LeuLys: 4.542 ± 0.543
6.013LeuLeu: 6.013 ± 0.684
1.919LeuMet: 1.919 ± 0.326
2.495LeuAsn: 2.495 ± 0.51
3.454LeuPro: 3.454 ± 0.455
2.431LeuGln: 2.431 ± 0.38
6.525LeuArg: 6.525 ± 0.712
6.205LeuSer: 6.205 ± 0.58
5.565LeuThr: 5.565 ± 0.513
4.734LeuVal: 4.734 ± 0.519
0.896LeuTrp: 0.896 ± 0.311
3.326LeuTyr: 3.326 ± 0.412
0.0LeuXaa: 0.0 ± 0.0
Met
1.983MetAla: 1.983 ± 0.305
0.128MetCys: 0.128 ± 0.087
1.279MetAsp: 1.279 ± 0.241
1.663MetGlu: 1.663 ± 0.307
0.832MetPhe: 0.832 ± 0.188
1.215MetGly: 1.215 ± 0.269
0.448MetHis: 0.448 ± 0.148
1.215MetIle: 1.215 ± 0.259
1.535MetLys: 1.535 ± 0.319
2.239MetLeu: 2.239 ± 0.401
0.64MetMet: 0.64 ± 0.206
1.343MetAsn: 1.343 ± 0.264
1.215MetPro: 1.215 ± 0.288
0.896MetGln: 0.896 ± 0.218
2.303MetArg: 2.303 ± 0.344
2.111MetSer: 2.111 ± 0.312
1.535MetThr: 1.535 ± 0.268
1.215MetVal: 1.215 ± 0.27
0.256MetTrp: 0.256 ± 0.115
0.832MetTyr: 0.832 ± 0.194
0.0MetXaa: 0.0 ± 0.0
Asn
2.815AsnAla: 2.815 ± 0.385
0.448AsnCys: 0.448 ± 0.197
1.983AsnAsp: 1.983 ± 0.317
3.71AsnGlu: 3.71 ± 0.364
0.96AsnPhe: 0.96 ± 0.216
3.71AsnGly: 3.71 ± 0.454
0.768AsnHis: 0.768 ± 0.23
1.343AsnIle: 1.343 ± 0.294
2.047AsnLys: 2.047 ± 0.41
3.07AsnLeu: 3.07 ± 0.4
0.832AsnMet: 0.832 ± 0.257
1.663AsnAsn: 1.663 ± 0.324
2.047AsnPro: 2.047 ± 0.4
0.896AsnGln: 0.896 ± 0.263
2.879AsnArg: 2.879 ± 0.575
2.047AsnSer: 2.047 ± 0.36
1.791AsnThr: 1.791 ± 0.279
2.495AsnVal: 2.495 ± 0.382
0.64AsnTrp: 0.64 ± 0.22
1.151AsnTyr: 1.151 ± 0.218
0.0AsnXaa: 0.0 ± 0.0
Pro
2.687ProAla: 2.687 ± 0.474
0.192ProCys: 0.192 ± 0.099
2.879ProAsp: 2.879 ± 0.343
4.03ProGlu: 4.03 ± 0.456
1.023ProPhe: 1.023 ± 0.246
2.303ProGly: 2.303 ± 0.425
0.512ProHis: 0.512 ± 0.158
2.623ProIle: 2.623 ± 0.34
2.559ProLys: 2.559 ± 0.343
2.623ProLeu: 2.623 ± 0.487
1.215ProMet: 1.215 ± 0.279
1.983ProAsn: 1.983 ± 0.375
0.896ProPro: 0.896 ± 0.312
0.896ProGln: 0.896 ± 0.203
1.791ProArg: 1.791 ± 0.347
1.599ProSer: 1.599 ± 0.289
2.559ProThr: 2.559 ± 0.437
2.111ProVal: 2.111 ± 0.399
0.384ProTrp: 0.384 ± 0.166
1.535ProTyr: 1.535 ± 0.275
0.0ProXaa: 0.0 ± 0.0
Gln
2.815GlnAla: 2.815 ± 0.497
0.256GlnCys: 0.256 ± 0.15
1.407GlnAsp: 1.407 ± 0.371
2.175GlnGlu: 2.175 ± 0.356
1.087GlnPhe: 1.087 ± 0.29
1.279GlnGly: 1.279 ± 0.297
0.512GlnHis: 0.512 ± 0.173
2.047GlnIle: 2.047 ± 0.28
2.175GlnLys: 2.175 ± 0.339
2.942GlnLeu: 2.942 ± 0.355
0.96GlnMet: 0.96 ± 0.255
1.599GlnAsn: 1.599 ± 0.313
0.512GlnPro: 0.512 ± 0.167
0.896GlnGln: 0.896 ± 0.272
2.367GlnArg: 2.367 ± 0.393
1.279GlnSer: 1.279 ± 0.239
2.367GlnThr: 2.367 ± 0.478
1.599GlnVal: 1.599 ± 0.262
0.448GlnTrp: 0.448 ± 0.178
1.215GlnTyr: 1.215 ± 0.216
0.0GlnXaa: 0.0 ± 0.0
Arg
4.542ArgAla: 4.542 ± 0.589
0.576ArgCys: 0.576 ± 0.201
4.158ArgAsp: 4.158 ± 0.513
6.205ArgGlu: 6.205 ± 0.754
2.367ArgPhe: 2.367 ± 0.508
3.582ArgGly: 3.582 ± 0.409
1.215ArgHis: 1.215 ± 0.257
4.094ArgIle: 4.094 ± 0.482
6.717ArgLys: 6.717 ± 0.631
6.397ArgLeu: 6.397 ± 0.68
2.047ArgMet: 2.047 ± 0.361
3.966ArgAsn: 3.966 ± 0.594
2.175ArgPro: 2.175 ± 0.311
2.495ArgGln: 2.495 ± 0.344
4.03ArgArg: 4.03 ± 0.545
2.559ArgSer: 2.559 ± 0.422
2.751ArgThr: 2.751 ± 0.374
3.326ArgVal: 3.326 ± 0.615
1.087ArgTrp: 1.087 ± 0.247
2.239ArgTyr: 2.239 ± 0.355
0.0ArgXaa: 0.0 ± 0.0
Ser
4.03SerAla: 4.03 ± 0.48
0.512SerCys: 0.512 ± 0.174
2.751SerAsp: 2.751 ± 0.328
4.35SerGlu: 4.35 ± 0.501
2.367SerPhe: 2.367 ± 0.33
4.478SerGly: 4.478 ± 0.46
0.896SerHis: 0.896 ± 0.267
3.262SerIle: 3.262 ± 0.461
3.966SerLys: 3.966 ± 0.457
3.582SerLeu: 3.582 ± 0.413
1.279SerMet: 1.279 ± 0.279
1.471SerAsn: 1.471 ± 0.293
2.367SerPro: 2.367 ± 0.427
1.791SerGln: 1.791 ± 0.315
2.815SerArg: 2.815 ± 0.479
3.326SerSer: 3.326 ± 0.454
2.303SerThr: 2.303 ± 0.393
4.158SerVal: 4.158 ± 0.535
0.768SerTrp: 0.768 ± 0.213
2.495SerTyr: 2.495 ± 0.32
0.0SerXaa: 0.0 ± 0.0
Thr
4.734ThrAla: 4.734 ± 0.587
0.448ThrCys: 0.448 ± 0.146
4.03ThrAsp: 4.03 ± 0.417
4.286ThrGlu: 4.286 ± 0.601
2.495ThrPhe: 2.495 ± 0.335
4.989ThrGly: 4.989 ± 0.534
0.896ThrHis: 0.896 ± 0.217
3.07ThrIle: 3.07 ± 0.451
3.902ThrLys: 3.902 ± 0.575
3.582ThrLeu: 3.582 ± 0.456
1.151ThrMet: 1.151 ± 0.317
1.471ThrAsn: 1.471 ± 0.285
2.303ThrPro: 2.303 ± 0.328
1.919ThrGln: 1.919 ± 0.325
2.815ThrArg: 2.815 ± 0.401
2.687ThrSer: 2.687 ± 0.376
2.239ThrThr: 2.239 ± 0.363
3.902ThrVal: 3.902 ± 0.5
0.768ThrTrp: 0.768 ± 0.225
2.687ThrTyr: 2.687 ± 0.387
0.0ThrXaa: 0.0 ± 0.0
Val
6.013ValAla: 6.013 ± 0.547
0.96ValCys: 0.96 ± 0.324
4.222ValAsp: 4.222 ± 0.388
4.989ValGlu: 4.989 ± 0.608
2.623ValPhe: 2.623 ± 0.397
4.158ValGly: 4.158 ± 0.564
1.279ValHis: 1.279 ± 0.283
4.478ValIle: 4.478 ± 0.515
5.053ValLys: 5.053 ± 0.576
6.013ValLeu: 6.013 ± 0.578
1.407ValMet: 1.407 ± 0.299
2.175ValAsn: 2.175 ± 0.425
2.495ValPro: 2.495 ± 0.48
1.919ValGln: 1.919 ± 0.315
4.35ValArg: 4.35 ± 0.533
3.774ValSer: 3.774 ± 0.407
4.158ValThr: 4.158 ± 0.577
4.222ValVal: 4.222 ± 0.541
0.832ValTrp: 0.832 ± 0.188
2.623ValTyr: 2.623 ± 0.373
0.0ValXaa: 0.0 ± 0.0
Trp
0.96TrpAla: 0.96 ± 0.236
0.064TrpCys: 0.064 ± 0.056
0.96TrpAsp: 0.96 ± 0.267
1.279TrpGlu: 1.279 ± 0.309
0.512TrpPhe: 0.512 ± 0.164
0.64TrpGly: 0.64 ± 0.191
0.576TrpHis: 0.576 ± 0.198
0.832TrpIle: 0.832 ± 0.241
0.896TrpLys: 0.896 ± 0.245
1.343TrpLeu: 1.343 ± 0.287
0.32TrpMet: 0.32 ± 0.144
0.64TrpAsn: 0.64 ± 0.2
0.32TrpPro: 0.32 ± 0.141
0.832TrpGln: 0.832 ± 0.22
0.768TrpArg: 0.768 ± 0.215
0.704TrpSer: 0.704 ± 0.241
1.087TrpThr: 1.087 ± 0.234
1.343TrpVal: 1.343 ± 0.318
0.064TrpTrp: 0.064 ± 0.061
0.64TrpTyr: 0.64 ± 0.165
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.518TyrAla: 3.518 ± 0.394
0.256TyrCys: 0.256 ± 0.111
3.134TyrAsp: 3.134 ± 0.466
2.942TyrGlu: 2.942 ± 0.424
1.407TyrPhe: 1.407 ± 0.295
3.454TyrGly: 3.454 ± 0.461
0.576TyrHis: 0.576 ± 0.19
3.006TyrIle: 3.006 ± 0.474
2.815TyrLys: 2.815 ± 0.322
3.006TyrLeu: 3.006 ± 0.38
0.64TyrMet: 0.64 ± 0.194
0.576TyrAsn: 0.576 ± 0.191
1.407TyrPro: 1.407 ± 0.283
0.96TyrGln: 0.96 ± 0.201
3.198TyrArg: 3.198 ± 0.381
2.303TyrSer: 2.303 ± 0.405
2.111TyrThr: 2.111 ± 0.371
3.262TyrVal: 3.262 ± 0.456
0.64TyrTrp: 0.64 ± 0.207
1.727TyrTyr: 1.727 ± 0.325
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 78 proteins (15634 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski