Potexvirus sp.
Average proteome isoelectric point is 7.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345GSD3|A0A345GSD3_9VIRU ORF1.5 OS=Potexvirus sp. OX=2283800 PE=4 SV=1
MM1 pKa = 7.17 ARR3 pKa = 11.84 VRR5 pKa = 11.84 EE6 pKa = 4.24 VMSALTDD13 pKa = 3.89 PSLKK17 pKa = 10.68 AVIQEE22 pKa = 4.0 EE23 pKa = 4.8 AYY25 pKa = 9.95 KK26 pKa = 10.47 SIKK29 pKa = 10.28 LSLEE33 pKa = 4.03 ATKK36 pKa = 9.11 HH37 pKa = 5.09 TNPFAQTNEE46 pKa = 4.19 SADD49 pKa = 3.46 VLEE52 pKa = 4.81 NLGIMTNPLAVLPHH66 pKa = 5.49 THH68 pKa = 6.84 AADD71 pKa = 3.48 KK72 pKa = 11.08 ALEE75 pKa = 3.91 NDD77 pKa = 3.44 MYY79 pKa = 11.47 DD80 pKa = 3.38 VVSNYY85 pKa = 8.95 FDD87 pKa = 3.64 RR88 pKa = 11.84 SEE90 pKa = 5.08 PITFYY95 pKa = 11.33 YY96 pKa = 8.74 MKK98 pKa = 9.69 PGKK101 pKa = 9.97 LGRR104 pKa = 11.84 FRR106 pKa = 11.84 RR107 pKa = 11.84 GPQQRR112 pKa = 11.84 NYY114 pKa = 10.38 FINTIFEE121 pKa = 4.4 PKK123 pKa = 10.23 DD124 pKa = 3.28 VVRR127 pKa = 11.84 YY128 pKa = 9.28 PEE130 pKa = 3.69 ATIYY134 pKa = 10.82 KK135 pKa = 9.44 SFHH138 pKa = 6.57 DD139 pKa = 4.2 QPCTTEE145 pKa = 3.8 KK146 pKa = 11.21 AFMGDD151 pKa = 3.31 TLHH154 pKa = 6.78 YY155 pKa = 9.37 WDD157 pKa = 5.84 PDD159 pKa = 3.27 HH160 pKa = 7.47 LAQLFMNSPKK170 pKa = 10.53 LNQLYY175 pKa = 8.4 ATMVLPAEE183 pKa = 4.76 AYY185 pKa = 9.9 YY186 pKa = 10.65 KK187 pKa = 9.98 IDD189 pKa = 3.14 SLYY192 pKa = 9.39 PQIYY196 pKa = 6.58 TLKK199 pKa = 9.18 YY200 pKa = 8.08 VKK202 pKa = 9.97 EE203 pKa = 4.05 HH204 pKa = 6.27 FMYY207 pKa = 10.85 LPGGHH212 pKa = 7.26 AGGAYY217 pKa = 9.28 CHH219 pKa = 6.69 HH220 pKa = 6.89 RR221 pKa = 11.84 RR222 pKa = 11.84 QLGWLRR228 pKa = 11.84 IGKK231 pKa = 9.73 ISRR234 pKa = 11.84 FGGRR238 pKa = 11.84 LTLTLQILEE247 pKa = 4.45 SKK249 pKa = 9.96 AAHH252 pKa = 6.28 HH253 pKa = 6.56 LFLITRR259 pKa = 11.84 GDD261 pKa = 3.79 LQTPLYY267 pKa = 9.67 RR268 pKa = 11.84 TFTQSTPYY276 pKa = 10.71 VLLPRR281 pKa = 11.84 IFLPARR287 pKa = 11.84 YY288 pKa = 9.09 NCRR291 pKa = 11.84 TPIPLEE297 pKa = 4.08 LASKK301 pKa = 9.99 MFMYY305 pKa = 10.25 CKK307 pKa = 10.41 SLGEE311 pKa = 4.05 VKK313 pKa = 9.98 MRR315 pKa = 11.84 DD316 pKa = 3.41 LYY318 pKa = 11.56 AKK320 pKa = 9.49 LRR322 pKa = 11.84 QMLPSKK328 pKa = 10.5 EE329 pKa = 3.91 LVKK332 pKa = 11.01 YY333 pKa = 10.63 RR334 pKa = 11.84 GDD336 pKa = 3.42 DD337 pKa = 3.39 LVFITNYY344 pKa = 9.9 FYY346 pKa = 11.06 LISRR350 pKa = 11.84 LDD352 pKa = 3.37 SMTCFDD358 pKa = 5.46 QILSGNLLQKK368 pKa = 10.63 ALRR371 pKa = 11.84 PVRR374 pKa = 11.84 AWFQEE379 pKa = 3.81 VAMKK383 pKa = 10.55 IFGRR387 pKa = 11.84 HH388 pKa = 5.35 EE389 pKa = 3.79 FTQLVEE395 pKa = 4.08 ALQWTPIDD403 pKa = 3.48 LTFEE407 pKa = 4.13 VTIDD411 pKa = 3.85 QPSDD415 pKa = 2.52 WFTNMLNSTFGPPLPNEE432 pKa = 4.51 LNPSPKK438 pKa = 9.54 TPPGFEE444 pKa = 4.32 TEE446 pKa = 4.53 DD447 pKa = 3.66 PTTEE451 pKa = 3.7 AANLFEE457 pKa = 4.64 AWDD460 pKa = 5.01 RR461 pKa = 11.84 INKK464 pKa = 8.45 IHH466 pKa = 6.96 GPPAPTTFPEE476 pKa = 4.39 EE477 pKa = 4.03 GTTTVMQEE485 pKa = 3.93 DD486 pKa = 5.08 TPSEE490 pKa = 4.15 THH492 pKa = 6.94 AEE494 pKa = 3.77 PAAPKK499 pKa = 10.28 EE500 pKa = 3.95 LDD502 pKa = 3.54 AEE504 pKa = 4.21 ATAAVQDD511 pKa = 4.34 DD512 pKa = 4.11 DD513 pKa = 4.4 EE514 pKa = 4.74 EE515 pKa = 4.83 RR516 pKa = 11.84 PKK518 pKa = 10.73 PSWASEE524 pKa = 3.95 VSEE527 pKa = 4.65 EE528 pKa = 4.98 IPVQVQPEE536 pKa = 4.37 GTPASLPIKK545 pKa = 10.03 VSKK548 pKa = 10.18 PYY550 pKa = 8.82 PTEE553 pKa = 3.68 VYY555 pKa = 10.47 NAVHH559 pKa = 5.86 QLVMDD564 pKa = 4.69 TLPMPDD570 pKa = 3.24 KK571 pKa = 11.1 LKK573 pKa = 10.73 GRR575 pKa = 11.84 RR576 pKa = 11.84 AGLFSRR582 pKa = 11.84 NSKK585 pKa = 9.27 VKK587 pKa = 9.09 EE588 pKa = 3.67 YY589 pKa = 10.69 SYY591 pKa = 11.84 GKK593 pKa = 9.1 IKK595 pKa = 10.44 HH596 pKa = 5.94 VSSPWRR602 pKa = 11.84 DD603 pKa = 3.37 SLDD606 pKa = 3.61 QIGKK610 pKa = 8.59 MLDD613 pKa = 3.55 LDD615 pKa = 4.29 LSSFDD620 pKa = 3.29 HH621 pKa = 7.0 CLVQFFEE628 pKa = 4.72 DD629 pKa = 3.35 QALIPPHH636 pKa = 7.05 ADD638 pKa = 3.11 NEE640 pKa = 4.56 SLIQKK645 pKa = 9.65 HH646 pKa = 5.44 SRR648 pKa = 11.84 ILTVSLGSALLTIQPRR664 pKa = 11.84 LSSSNAPPVSIPLEE678 pKa = 4.31 GCTVYY683 pKa = 11.27 VMEE686 pKa = 5.06 EE687 pKa = 3.96 TCQDD691 pKa = 3.82 DD692 pKa = 3.91 FLHH695 pKa = 6.88 GIKK698 pKa = 10.44 ALEE701 pKa = 4.16 SGRR704 pKa = 11.84 LSLTFRR710 pKa = 11.84 RR711 pKa = 11.84 SVHH714 pKa = 5.46 FEE716 pKa = 3.86 KK717 pKa = 10.87 EE718 pKa = 4.44 SFDD721 pKa = 4.96 HH722 pKa = 5.9 SLPWAAWIPILRR734 pKa = 11.84 SLGFRR739 pKa = 11.84 GNEE742 pKa = 3.77 RR743 pKa = 11.84 QINPGDD749 pKa = 3.4 QSLIMPISEE758 pKa = 4.6 INNGLPKK765 pKa = 10.43 ASCPTKK771 pKa = 10.23 EE772 pKa = 3.76 WTEE775 pKa = 3.83 LLSRR779 pKa = 11.84 LHH781 pKa = 6.43 RR782 pKa = 11.84 LPVPFHH788 pKa = 6.95 PSLEE792 pKa = 4.04 RR793 pKa = 11.84 AKK795 pKa = 11.12 AFGSDD800 pKa = 3.35 IKK802 pKa = 10.9 NCRR805 pKa = 11.84 IGKK808 pKa = 9.23 LLTHH812 pKa = 6.06 QPEE815 pKa = 3.95 VWRR818 pKa = 11.84 NAFGRR823 pKa = 11.84 RR824 pKa = 11.84 TEE826 pKa = 3.98 EE827 pKa = 3.79 QPRR830 pKa = 11.84 EE831 pKa = 3.79 LALSLIHH838 pKa = 6.69 GAGGSGKK845 pKa = 10.28 SYY847 pKa = 10.51 AIQDD851 pKa = 3.91 FLRR854 pKa = 11.84 AHH856 pKa = 7.17 PDD858 pKa = 2.93 INVTIILPTNEE869 pKa = 5.1 LRR871 pKa = 11.84 LDD873 pKa = 3.68 WVSKK877 pKa = 11.0 LPATPLANIKK887 pKa = 9.47 TYY889 pKa = 10.74 EE890 pKa = 4.06 KK891 pKa = 10.65 ALLSNVHH898 pKa = 5.86 EE899 pKa = 4.4 VVILDD904 pKa = 4.95 DD905 pKa = 4.04 YY906 pKa = 11.77 GKK908 pKa = 10.71 LPAGFVEE915 pKa = 4.8 ALVCVSPALKK925 pKa = 10.53 LIIATGDD932 pKa = 3.54 SQQSVHH938 pKa = 6.63 HH939 pKa = 6.35 EE940 pKa = 4.39 SNEE943 pKa = 3.58 NSSIYY948 pKa = 10.21 KK949 pKa = 10.14 LPPLIQIVQPLCRR962 pKa = 11.84 YY963 pKa = 8.65 YY964 pKa = 10.45 INATHH969 pKa = 7.61 RR970 pKa = 11.84 NRR972 pKa = 11.84 KK973 pKa = 8.28 QLANMLGVYY982 pKa = 9.67 SEE984 pKa = 4.3 NVGDD988 pKa = 4.53 FRR990 pKa = 11.84 ITHH993 pKa = 5.56 GTSPIQGVHH1002 pKa = 6.4 LLVPSIFKK1010 pKa = 8.96 KK1011 pKa = 9.05 TAFAEE1016 pKa = 4.32 MGHH1019 pKa = 6.05 KK1020 pKa = 10.34 VSTYY1024 pKa = 10.35 SGCQGITAEE1033 pKa = 4.4 RR1034 pKa = 11.84 VQILLDD1040 pKa = 3.8 QDD1042 pKa = 3.49 THH1044 pKa = 7.33 LCTKK1048 pKa = 10.05 QVLYY1052 pKa = 9.54 TALSRR1057 pKa = 11.84 AVHH1060 pKa = 6.58 SIHH1063 pKa = 7.39 FIDD1066 pKa = 4.7 TGTKK1070 pKa = 10.31 LNSFWEE1076 pKa = 4.34 KK1077 pKa = 11.06 LDD1079 pKa = 3.3 ATPYY1083 pKa = 10.82 LKK1085 pKa = 9.99 TFLRR1089 pKa = 11.84 TIRR1092 pKa = 11.84 EE1093 pKa = 4.15 EE1094 pKa = 3.85 KK1095 pKa = 9.79 LKK1097 pKa = 10.65 EE1098 pKa = 3.95 MEE1100 pKa = 4.24 VVEE1103 pKa = 4.33 PEE1105 pKa = 3.96 IAEE1108 pKa = 4.03 PAPPNTHH1115 pKa = 6.31 FPVEE1119 pKa = 3.89 NSKK1122 pKa = 10.84 NFFDD1126 pKa = 5.06 NVTEE1130 pKa = 4.01 NMGEE1134 pKa = 3.94 KK1135 pKa = 10.15 FEE1137 pKa = 5.22 RR1138 pKa = 11.84 EE1139 pKa = 3.59 IFKK1142 pKa = 10.84 ADD1144 pKa = 3.16 TGFSNCVQTEE1154 pKa = 4.09 DD1155 pKa = 4.46 QLVQMFAHH1163 pKa = 5.62 QQAKK1167 pKa = 10.83 DD1168 pKa = 3.52 EE1169 pKa = 4.39 TLFWATIEE1177 pKa = 4.0 ARR1179 pKa = 11.84 LTITNPKK1186 pKa = 9.84 VNFTEE1191 pKa = 5.3 FYY1193 pKa = 8.74 NKK1195 pKa = 10.19 KK1196 pKa = 10.23 KK1197 pKa = 10.08 IGEE1200 pKa = 4.18 ILFEE1204 pKa = 4.21 NYY1206 pKa = 8.35 KK1207 pKa = 10.52 RR1208 pKa = 11.84 AMQLPNDD1215 pKa = 3.66 PVAFSRR1221 pKa = 11.84 EE1222 pKa = 3.98 LWDD1225 pKa = 3.34 VCADD1229 pKa = 3.9 EE1230 pKa = 4.32 VQKK1233 pKa = 10.0 TYY1235 pKa = 10.96 LSKK1238 pKa = 10.22 PLHH1241 pKa = 6.02 MIKK1244 pKa = 10.33 NGQNRR1249 pKa = 11.84 QSPDD1253 pKa = 3.17 YY1254 pKa = 9.19 PKK1256 pKa = 11.21 NFITLFLKK1264 pKa = 9.87 SQWVQKK1270 pKa = 10.43 LEE1272 pKa = 3.94 KK1273 pKa = 10.56 LGLPKK1278 pKa = 10.15 IKK1280 pKa = 9.99 PGQTIASFHH1289 pKa = 6.58 QITVMLYY1296 pKa = 8.32 GTMARR1301 pKa = 11.84 YY1302 pKa = 9.06 LRR1304 pKa = 11.84 RR1305 pKa = 11.84 TRR1307 pKa = 11.84 EE1308 pKa = 3.7 TFQPANIFINCEE1320 pKa = 3.73 RR1321 pKa = 11.84 TPEE1324 pKa = 4.43 DD1325 pKa = 2.93 MSKK1328 pKa = 9.85 WVRR1331 pKa = 11.84 EE1332 pKa = 3.82 HH1333 pKa = 6.49 WNFTRR1338 pKa = 11.84 TSYY1341 pKa = 11.85 ANDD1344 pKa = 3.1 FTAFDD1349 pKa = 3.83 QSQDD1353 pKa = 3.0 GAMLQFEE1360 pKa = 4.35 IMKK1363 pKa = 10.45 ARR1365 pKa = 11.84 FFNIPEE1371 pKa = 4.1 EE1372 pKa = 4.28 VIEE1375 pKa = 4.63 GYY1377 pKa = 10.18 IDD1379 pKa = 3.38 IKK1381 pKa = 11.21 CNAEE1385 pKa = 3.93 VFTGVLSIMRR1395 pKa = 11.84 LTGEE1399 pKa = 4.65 GPTFDD1404 pKa = 6.01 ANTEE1408 pKa = 4.03 CNIAFTHH1415 pKa = 5.68 TKK1417 pKa = 9.9 YY1418 pKa = 10.9 NIPKK1422 pKa = 9.8 EE1423 pKa = 4.15 CAQLFAGDD1431 pKa = 4.73 DD1432 pKa = 3.71 SALDD1436 pKa = 3.56 FSPEE1440 pKa = 3.94 VKK1442 pKa = 10.17 PSFRR1446 pKa = 11.84 LIEE1449 pKa = 4.31 TEE1451 pKa = 4.32 LSLTAKK1457 pKa = 9.98 PVLKK1461 pKa = 9.58 MQIQGQWAEE1470 pKa = 4.05 FCGMLITPLGVVKK1483 pKa = 10.68 DD1484 pKa = 4.63 PIKK1487 pKa = 10.78 LWASWVRR1494 pKa = 11.84 ARR1496 pKa = 11.84 QTGKK1500 pKa = 10.42 LKK1502 pKa = 10.56 EE1503 pKa = 4.19 VADD1506 pKa = 4.15 SYY1508 pKa = 11.97 EE1509 pKa = 4.37 LDD1511 pKa = 3.34 LCLAYY1516 pKa = 8.48 QHH1518 pKa = 7.09 RR1519 pKa = 11.84 DD1520 pKa = 2.98 LLQEE1524 pKa = 4.13 VFDD1527 pKa = 4.11 EE1528 pKa = 4.68 RR1529 pKa = 11.84 QSEE1532 pKa = 4.22 AHH1534 pKa = 6.3 FNTVRR1539 pKa = 11.84 EE1540 pKa = 4.28 IILYY1544 pKa = 8.37 TKK1546 pKa = 10.15 GRR1548 pKa = 11.84 RR1549 pKa = 11.84 LATYY1553 pKa = 10.25 NGG1555 pKa = 3.56
Molecular weight: 177.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.054
IPC2_protein 6.008
IPC_protein 6.084
Toseland 6.465
ProMoST 6.478
Dawson 6.402
Bjellqvist 6.402
Wikipedia 6.415
Rodwell 6.389
Grimsley 6.62
Solomon 6.402
Lehninger 6.389
Nozaki 6.678
DTASelect 6.854
Thurlkill 6.883
EMBOSS 6.854
Sillero 6.781
Patrickios 4.24
IPC_peptide 6.415
IPC2_peptide 6.693
IPC2.peptide.svr19 6.688
Protein with the highest isoelectric point:
>tr|A0A345GSD4|A0A345GSD4_9VIRU ORF2 OS=Potexvirus sp. OX=2283800 PE=4 SV=1
MM1 pKa = 7.06 QRR3 pKa = 11.84 TATGSSWPTCWAFTLKK19 pKa = 9.16 MWEE22 pKa = 3.95 ISGSPTEE29 pKa = 4.1 LAQSKK34 pKa = 10.6 GCTFWSRR41 pKa = 11.84 PSSRR45 pKa = 11.84 KK46 pKa = 9.36 LRR48 pKa = 11.84 SLRR51 pKa = 11.84 WDD53 pKa = 3.81 TKK55 pKa = 10.56 CQLTQAVKK63 pKa = 10.38 ASRR66 pKa = 11.84 LSVCKK71 pKa = 10.43 FSLTKK76 pKa = 10.52 TPTCAPNRR84 pKa = 11.84 FSTLHH89 pKa = 5.8 SPEE92 pKa = 4.93 RR93 pKa = 11.84 STQSTLLTQGPNN105 pKa = 2.98
Molecular weight: 11.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.274
IPC2_protein 9.399
IPC_protein 9.531
Toseland 10.73
ProMoST 10.204
Dawson 10.774
Bjellqvist 10.394
Wikipedia 10.877
Rodwell 11.199
Grimsley 10.774
Solomon 10.862
Lehninger 10.862
Nozaki 10.745
DTASelect 10.365
Thurlkill 10.701
EMBOSS 11.111
Sillero 10.716
Patrickios 10.979
IPC_peptide 10.877
IPC2_peptide 9.677
IPC2.peptide.svr19 8.412
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2032
105
1555
508.0
57.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.644 ± 0.233
1.673 ± 0.521
4.528 ± 0.601
6.201 ± 1.722
4.872 ± 0.375
4.921 ± 0.581
3.002 ± 0.416
5.364 ± 0.705
5.561 ± 0.822
10.482 ± 0.844
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.165 ± 0.415
3.937 ± 0.426
6.841 ± 0.43
4.134 ± 0.74
5.364 ± 0.494
7.333 ± 2.334
7.333 ± 1.077
5.217 ± 0.965
1.427 ± 0.415
3.002 ± 0.728
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here