Streptococcus phage Javan14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6ARI9|A0A4D6ARI9_9CAUD Uncharacterized protein OS=Streptococcus phage Javan14 OX=2548001 GN=Javan14_0022 PE=4 SV=1
MM1 pKa = 7.77EE2 pKa = 4.53LTITQCLILLPFILFLLYY20 pKa = 10.67LVLTTDD26 pKa = 3.11SSFEE30 pKa = 3.76IDD32 pKa = 2.86IEE34 pKa = 4.42EE35 pKa = 4.2PVEE38 pKa = 4.06EE39 pKa = 4.59VEE41 pKa = 4.41LNPYY45 pKa = 9.0YY46 pKa = 10.85GAVIQNQGKK55 pKa = 9.65HH56 pKa = 4.42YY57 pKa = 10.94

Molecular weight: 6.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AR51|A0A4D6AR51_9CAUD Uncharacterized protein OS=Streptococcus phage Javan14 OX=2548001 GN=Javan14_0057 PE=4 SV=1
MM1 pKa = 7.12GAEE4 pKa = 4.02LKK6 pKa = 11.0GVDD9 pKa = 3.6EE10 pKa = 5.06LLANMEE16 pKa = 4.58KK17 pKa = 10.66KK18 pKa = 10.61LGSAKK23 pKa = 10.24VNRR26 pKa = 11.84VVNKK30 pKa = 9.76ALKK33 pKa = 10.19EE34 pKa = 3.82IGKK37 pKa = 9.21EE38 pKa = 3.88LEE40 pKa = 4.08PSFKK44 pKa = 10.77GAISVYY50 pKa = 8.67RR51 pKa = 11.84KK52 pKa = 10.1SGATVEE58 pKa = 4.41SAVVSGIKK66 pKa = 9.83RR67 pKa = 11.84EE68 pKa = 3.92EE69 pKa = 4.73GIPKK73 pKa = 10.07VKK75 pKa = 10.54LGFQAPRR82 pKa = 11.84WNIVHH87 pKa = 6.43LQEE90 pKa = 6.01LEE92 pKa = 4.27YY93 pKa = 10.74GWKK96 pKa = 9.75HH97 pKa = 4.58NRR99 pKa = 11.84RR100 pKa = 11.84GVGVIRR106 pKa = 11.84RR107 pKa = 11.84YY108 pKa = 10.68SDD110 pKa = 2.97ILEE113 pKa = 4.49TIYY116 pKa = 10.64PRR118 pKa = 11.84GIRR121 pKa = 11.84DD122 pKa = 3.43KK123 pKa = 11.4LKK125 pKa = 11.06GGFDD129 pKa = 3.73GG130 pKa = 5.39

Molecular weight: 14.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

12690

38

1361

195.2

22.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.336 ± 0.6

0.52 ± 0.085

6.588 ± 0.29

7.549 ± 0.493

4.271 ± 0.322

5.981 ± 0.518

1.261 ± 0.152

7.266 ± 0.348

9.212 ± 0.495

8.258 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.38 ± 0.193

5.95 ± 0.256

2.797 ± 0.159

3.578 ± 0.225

4.027 ± 0.265

6.47 ± 0.295

6.099 ± 0.378

6.06 ± 0.237

1.095 ± 0.081

4.303 ± 0.321
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski