Aquimarina algiphila
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6064 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A554VIL8|A0A554VIL8_9FLAO Uncharacterized protein OS=Aquimarina algiphila OX=2047982 GN=FOF46_15305 PE=4 SV=1
MM1 pKa = 7.3 NKK3 pKa = 8.26 KK4 pKa = 8.54 TYY6 pKa = 10.25 LIVVILLTISKK17 pKa = 10.41 GLFAQTMTLTSNAPSNTICRR37 pKa = 11.84 GDD39 pKa = 3.85 IIQVDD44 pKa = 4.21 VNITPPNGASGYY56 pKa = 9.43 NWTNVTTGQFIKK68 pKa = 10.62 SVNGNAGTFHH78 pKa = 7.52 LFTGVDD84 pKa = 3.27 RR85 pKa = 11.84 LLTNSTIRR93 pKa = 11.84 VEE95 pKa = 4.4 AFGGTGGNLSQTIDD109 pKa = 3.85 IIVQQPANPGTEE121 pKa = 4.24 GNLLLCNKK129 pKa = 9.35 TGDD132 pKa = 3.39 IDD134 pKa = 6.3 LFTLLQGNPDD144 pKa = 4.2 RR145 pKa = 11.84 GGTWDD150 pKa = 3.99 PPLANGDD157 pKa = 3.59 RR158 pKa = 11.84 GTFTIGTDD166 pKa = 3.06 TPGIYY171 pKa = 9.35 RR172 pKa = 11.84 YY173 pKa = 8.8 MISATAPCSDD183 pKa = 3.44 SEE185 pKa = 4.41 SRR187 pKa = 11.84 VVVKK191 pKa = 10.55 EE192 pKa = 4.01 CFNDD196 pKa = 4.2 DD197 pKa = 3.63 FDD199 pKa = 6.03 NDD201 pKa = 3.81 GVNNKK206 pKa = 9.96 DD207 pKa = 5.79 DD208 pKa = 5.77 LDD210 pKa = 5.14 DD211 pKa = 5.77 DD212 pKa = 4.37 NDD214 pKa = 4.97 GILDD218 pKa = 3.76 SVEE221 pKa = 4.59 NSACTSATLTEE232 pKa = 4.34 SSPVVDD238 pKa = 3.69 IDD240 pKa = 3.88 FGTGNTPTTDD250 pKa = 3.64 PNIQGHH256 pKa = 4.65 QHH258 pKa = 5.51 NPNWPDD264 pKa = 3.18 DD265 pKa = 3.69 GFYY268 pKa = 11.22 NVANSLYY275 pKa = 9.25 MFNSANFDD283 pKa = 3.26 VWFITTDD290 pKa = 3.6 TNPVPHH296 pKa = 7.05 SDD298 pKa = 3.72 GNGDD302 pKa = 3.4 VNGRR306 pKa = 11.84 FLAVNIASNFGNKK319 pKa = 7.89 VLYY322 pKa = 9.05 EE323 pKa = 4.18 LKK325 pKa = 10.41 DD326 pKa = 3.4 IPISAGIQYY335 pKa = 10.08 NFRR338 pKa = 11.84 IDD340 pKa = 3.68 MVGLCDD346 pKa = 4.59 RR347 pKa = 11.84 GCADD351 pKa = 4.29 VPALDD356 pKa = 5.7 LEE358 pKa = 5.61 IIDD361 pKa = 3.78 QTTGNTILSTTSAALGVANDD381 pKa = 4.76 DD382 pKa = 2.82 IWRR385 pKa = 11.84 TLLDD389 pKa = 5.53 DD390 pKa = 3.37 ITVPTNTLLTLRR402 pKa = 11.84 IRR404 pKa = 11.84 NQQLQGSNGNDD415 pKa = 2.72 IGIDD419 pKa = 3.59 NIRR422 pKa = 11.84 FAPLEE427 pKa = 4.35 CDD429 pKa = 3.14 SDD431 pKa = 4.25 RR432 pKa = 11.84 DD433 pKa = 3.8 EE434 pKa = 4.83 VPNFLDD440 pKa = 4.93 LDD442 pKa = 4.27 CDD444 pKa = 3.47 NDD446 pKa = 4.78 GIFDD450 pKa = 4.34 IIEE453 pKa = 4.33 AGRR456 pKa = 11.84 SDD458 pKa = 5.5 LDD460 pKa = 3.92 TNGDD464 pKa = 3.96 GIVDD468 pKa = 5.01 GIRR471 pKa = 11.84 DD472 pKa = 3.69 TDD474 pKa = 3.48 GDD476 pKa = 4.2 GIADD480 pKa = 3.42 VGVDD484 pKa = 3.66 VNGVPIAASGGLTPISSDD502 pKa = 3.16 GDD504 pKa = 3.84 GLPDD508 pKa = 4.19 YY509 pKa = 11.35 LDD511 pKa = 3.91 IDD513 pKa = 4.42 ADD515 pKa = 3.73 NDD517 pKa = 4.65 GIQDD521 pKa = 4.06 NIEE524 pKa = 4.07 GQATLTYY531 pKa = 9.9 IAPSGNDD538 pKa = 3.33 LNKK541 pKa = 10.79 NGVDD545 pKa = 3.58 DD546 pKa = 4.99 AYY548 pKa = 10.32 EE549 pKa = 4.42 GVGSITPVNTDD560 pKa = 2.75 TDD562 pKa = 4.08 TLPDD566 pKa = 3.97 YY567 pKa = 11.49 LDD569 pKa = 4.86 LNSDD573 pKa = 4.17 DD574 pKa = 5.57 DD575 pKa = 4.32 CLNDD579 pKa = 3.6 TVEE582 pKa = 5.45 AYY584 pKa = 10.25 DD585 pKa = 4.56 LNLDD589 pKa = 4.49 GISDD593 pKa = 3.75 ITAIGTDD600 pKa = 3.46 TDD602 pKa = 3.7 NDD604 pKa = 3.76 GMDD607 pKa = 3.24 NAFDD611 pKa = 4.26 IVILDD616 pKa = 4.0 ALTAITNPTDD626 pKa = 3.49 NGEE629 pKa = 4.45 LPTDD633 pKa = 4.18 LPNNHH638 pKa = 6.85 NIGGDD643 pKa = 3.06 VDD645 pKa = 4.2 FRR647 pKa = 11.84 EE648 pKa = 4.24 EE649 pKa = 3.91 FKK651 pKa = 11.16 KK652 pKa = 10.84 VDD654 pKa = 3.6 EE655 pKa = 4.78 KK656 pKa = 10.79 KK657 pKa = 8.85 TAGGCASGIPTINLFDD673 pKa = 4.2 SLVDD677 pKa = 3.48 AAIPGGTWSGPSVLTGGEE695 pKa = 4.06 SGTFNATTNVAGTYY709 pKa = 9.14 TYY711 pKa = 9.84 TLPTIGLCPSRR722 pKa = 11.84 KK723 pKa = 9.92 GEE725 pKa = 4.1 VKK727 pKa = 10.56 VSIISTSDD735 pKa = 2.81 PGTNGTLNTCVLVTTPINLFDD756 pKa = 4.25 SLGGTPDD763 pKa = 3.51 TGGSWTDD770 pKa = 3.27 PNGNPFGTTDD780 pKa = 3.3 QGVLNPSATGTLQGTYY796 pKa = 9.83 VYY798 pKa = 10.31 TIGTTGCSSSANVEE812 pKa = 4.03 VTINPGANAGLDD824 pKa = 3.53 NKK826 pKa = 10.25 VSFCSTQSSINLFDD840 pKa = 4.14 SLEE843 pKa = 4.27 GTPDD847 pKa = 3.33 TGGSWIDD854 pKa = 3.42 PNGNPFGTTDD864 pKa = 3.74 QGVLDD869 pKa = 4.55 PSLTTTLSGDD879 pKa = 3.57 YY880 pKa = 9.66 IYY882 pKa = 10.53 TVSATTCPIQDD893 pKa = 3.62 TAIVTVTIEE902 pKa = 3.79 IAPNPGTNSSLEE914 pKa = 3.82 ICTNASAINLFDD926 pKa = 4.06 SLRR929 pKa = 11.84 GAPTTGGTWTGPGGATFGTNDD950 pKa = 3.4 KK951 pKa = 11.69 GEE953 pKa = 4.42 FNPASDD959 pKa = 3.61 AAGTYY964 pKa = 9.19 TYY966 pKa = 7.58 TTGTIACNNTATITVTINPAANAGEE991 pKa = 4.11 DD992 pKa = 3.61 NKK994 pKa = 10.37 VSFCSTDD1001 pKa = 2.7 TTMNLFDD1008 pKa = 4.75 SLGGSPNIGGSWTDD1022 pKa = 3.26 PNGNPFGTTDD1032 pKa = 3.45 QGIIDD1037 pKa = 4.45 PSATTTLSGDD1047 pKa = 3.32 YY1048 pKa = 9.54 TYY1050 pKa = 10.8 TVSTGKK1056 pKa = 8.55 CTIPDD1061 pKa = 3.29 IAKK1064 pKa = 7.59 ITVVIEE1070 pKa = 4.03 TTPSISFDD1078 pKa = 3.12 KK1079 pKa = 8.29 TTCSDD1084 pKa = 3.34 DD1085 pKa = 3.72 RR1086 pKa = 11.84 NSYY1089 pKa = 10.4 SVSFTSNEE1097 pKa = 3.83 TWSITIDD1104 pKa = 3.63 PPNAGIIDD1112 pKa = 3.92 VTNSMITGIASDD1124 pKa = 3.74 TNIIITATNPANTTCEE1140 pKa = 3.87 DD1141 pKa = 3.96 TLNIIAPDD1149 pKa = 4.03 CTCPDD1154 pKa = 2.81 ITEE1157 pKa = 4.25 PTNPIHH1163 pKa = 7.07 QRR1165 pKa = 11.84 ICAGDD1170 pKa = 3.73 TNPVLSVTVLTDD1182 pKa = 3.2 QTANWYY1188 pKa = 7.21 TQDD1191 pKa = 3.12 GTLLVANTTTYY1202 pKa = 10.7 TPIEE1206 pKa = 4.25 TAIGTYY1212 pKa = 8.94 TYY1214 pKa = 10.94 SVEE1217 pKa = 4.95 AFDD1220 pKa = 5.58 NNEE1223 pKa = 3.91 KK1224 pKa = 10.66 CPSDD1228 pKa = 4.58 RR1229 pKa = 11.84 IPVFFDD1235 pKa = 2.65 IVTAPEE1241 pKa = 3.8 ITLSSDD1247 pKa = 3.25 SAICVSEE1254 pKa = 4.92 DD1255 pKa = 3.7 GVPLPTNDD1263 pKa = 3.96 FPFIDD1268 pKa = 3.89 TFLNTTDD1275 pKa = 4.5 FTFIWSFEE1283 pKa = 4.02 GTVIPGQNSSGIEE1296 pKa = 3.83 TLLPGTYY1303 pKa = 8.62 TVQYY1307 pKa = 8.01 TDD1309 pKa = 3.73 KK1310 pKa = 11.33 VSMCSDD1316 pKa = 3.62 TSSVTINAIEE1326 pKa = 4.67 GPQRR1330 pKa = 11.84 LDD1332 pKa = 3.16 LSLSLGTFADD1342 pKa = 3.87 HH1343 pKa = 7.04 NDD1345 pKa = 3.43 IIATVAGNSEE1355 pKa = 4.53 YY1356 pKa = 10.75 EE1357 pKa = 3.9 FSLDD1361 pKa = 3.2 NGSFQTEE1368 pKa = 3.95 NIFRR1372 pKa = 11.84 NVALGLHH1379 pKa = 5.99 EE1380 pKa = 4.24 VTVRR1384 pKa = 11.84 DD1385 pKa = 3.84 IYY1387 pKa = 11.61 GCGNLSKK1394 pKa = 10.3 EE1395 pKa = 4.08 IFVMGFPNFFTPNNDD1410 pKa = 4.37 GINDD1414 pKa = 3.78 TWNVIADD1421 pKa = 3.8 EE1422 pKa = 4.8 NLPEE1426 pKa = 4.0 MIIYY1430 pKa = 10.32 IFDD1433 pKa = 3.74 RR1434 pKa = 11.84 YY1435 pKa = 10.59 GKK1437 pKa = 10.18 LLSQLNPNGPGWDD1450 pKa = 3.74 GIYY1453 pKa = 10.46 NGRR1456 pKa = 11.84 TLPSTDD1462 pKa = 2.89 YY1463 pKa = 10.76 WFAAQFKK1470 pKa = 9.87 DD1471 pKa = 3.51 GSEE1474 pKa = 4.42 TYY1476 pKa = 10.07 RR1477 pKa = 11.84 SHH1479 pKa = 6.77 FTLKK1483 pKa = 10.44 RR1484 pKa = 3.41
Molecular weight: 157.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.685
IPC2_protein 3.605
IPC_protein 3.681
Toseland 3.427
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.834
Wikipedia 3.643
Rodwell 3.49
Grimsley 3.338
Solomon 3.681
Lehninger 3.643
Nozaki 3.795
DTASelect 4.101
Thurlkill 3.49
EMBOSS 3.656
Sillero 3.808
Patrickios 0.985
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.721
Protein with the highest isoelectric point:
>tr|A0A554VF87|A0A554VF87_9FLAO Uncharacterized protein OS=Aquimarina algiphila OX=2047982 GN=FOF46_21475 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 10.15 RR10 pKa = 11.84 KK11 pKa = 9.6 RR12 pKa = 11.84 KK13 pKa = 8.29 NKK15 pKa = 9.34 HH16 pKa = 4.03 GFRR19 pKa = 11.84 EE20 pKa = 4.2 RR21 pKa = 11.84 MASANGRR28 pKa = 11.84 KK29 pKa = 9.04 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.09 GRR39 pKa = 11.84 KK40 pKa = 7.97 KK41 pKa = 10.66 LSVSSEE47 pKa = 3.9 LRR49 pKa = 11.84 HH50 pKa = 5.43 KK51 pKa = 10.29 HH52 pKa = 5.04
Molecular weight: 6.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.051
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6064
0
6064
2018512
22
4042
332.9
37.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.706 ± 0.032
0.742 ± 0.011
5.737 ± 0.027
6.589 ± 0.031
5.153 ± 0.027
6.308 ± 0.034
1.78 ± 0.015
8.466 ± 0.028
7.899 ± 0.044
8.982 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.014 ± 0.016
6.332 ± 0.032
3.351 ± 0.021
3.423 ± 0.018
3.508 ± 0.021
6.691 ± 0.024
6.161 ± 0.043
5.855 ± 0.022
1.089 ± 0.012
4.214 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here