Deinbollia mosaic virus
Average proteome isoelectric point is 8.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A142C6K7|A0A142C6K7_9GEMI Capsid protein OS=Deinbollia mosaic virus OX=1812308 GN=AV1 PE=3 SV=1
MM1 pKa = 7.99 WDD3 pKa = 3.38 PLVNEE8 pKa = 5.24 FPDD11 pKa = 3.72 SVHH14 pKa = 6.81 GFRR17 pKa = 11.84 CMLAIKK23 pKa = 9.31 YY24 pKa = 8.48 LQAIEE29 pKa = 4.11 EE30 pKa = 4.56 TYY32 pKa = 10.77 EE33 pKa = 4.16 PNTLGHH39 pKa = 6.99 DD40 pKa = 5.01 LIRR43 pKa = 11.84 DD44 pKa = 4.58 LISVIRR50 pKa = 11.84 ARR52 pKa = 11.84 DD53 pKa = 3.49 YY54 pKa = 11.1 VEE56 pKa = 3.35 ATRR59 pKa = 11.84 RR60 pKa = 11.84 YY61 pKa = 8.32 NHH63 pKa = 5.68 FHH65 pKa = 6.31 SRR67 pKa = 11.84 LEE69 pKa = 4.38 GASKK73 pKa = 9.9 TEE75 pKa = 3.89 LRR77 pKa = 11.84 QPICQPCCCPYY88 pKa = 10.45 CPRR91 pKa = 11.84 HH92 pKa = 5.76 KK93 pKa = 9.6 QTSSMDD99 pKa = 3.66 LQAHH103 pKa = 4.65 VQKK106 pKa = 10.96 AQDD109 pKa = 3.55 VQNVQKK115 pKa = 10.64 PP116 pKa = 3.36
Molecular weight: 13.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.462
IPC2_protein 6.427
IPC_protein 6.605
Toseland 6.605
ProMoST 7.146
Dawson 7.117
Bjellqvist 6.985
Wikipedia 7.073
Rodwell 7.102
Grimsley 6.693
Solomon 7.146
Lehninger 7.161
Nozaki 7.351
DTASelect 7.41
Thurlkill 7.439
EMBOSS 7.468
Sillero 7.541
Patrickios 3.757
IPC_peptide 7.161
IPC2_peptide 7.249
IPC2.peptide.svr19 7.066
Protein with the highest isoelectric point:
>tr|A0A142C6K8|A0A142C6K8_9GEMI AC4 OS=Deinbollia mosaic virus OX=1812308 GN=AC4 PE=3 SV=1
MM1 pKa = 7.7 SKK3 pKa = 10.44 RR4 pKa = 11.84 PGDD7 pKa = 3.89 IIISTPASKK16 pKa = 10.34 VRR18 pKa = 11.84 RR19 pKa = 11.84 KK20 pKa = 10.58 LNFDD24 pKa = 3.17 SPYY27 pKa = 9.22 ASRR30 pKa = 11.84 VAVPIVQGTNRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 AWTYY47 pKa = 9.4 RR48 pKa = 11.84 PMYY51 pKa = 9.33 RR52 pKa = 11.84 KK53 pKa = 9.49 PKK55 pKa = 7.48 MYY57 pKa = 10.84 RR58 pKa = 11.84 MFRR61 pKa = 11.84 SLDD64 pKa = 3.4 VPRR67 pKa = 11.84 GCEE70 pKa = 4.36 GPCKK74 pKa = 9.32 VQSYY78 pKa = 6.99 EE79 pKa = 3.67 QRR81 pKa = 11.84 DD82 pKa = 4.05 DD83 pKa = 3.72 VKK85 pKa = 9.5 HH86 pKa = 5.48 TGIVRR91 pKa = 11.84 CVSDD95 pKa = 3.36 VTRR98 pKa = 11.84 GPGITHH104 pKa = 6.76 RR105 pKa = 11.84 VGKK108 pKa = 9.5 RR109 pKa = 11.84 FCVKK113 pKa = 10.3 SIYY116 pKa = 10.16 ILGKK120 pKa = 9.79 VWMDD124 pKa = 4.63 DD125 pKa = 3.63 NIKK128 pKa = 10.13 KK129 pKa = 9.97 QNHH132 pKa = 4.98 TNQVMFFLVRR142 pKa = 11.84 DD143 pKa = 3.69 RR144 pKa = 11.84 RR145 pKa = 11.84 PYY147 pKa = 8.77 GTSPQDD153 pKa = 3.2 FGQVFNMFDD162 pKa = 3.99 NEE164 pKa = 4.11 PSTATVKK171 pKa = 10.81 NDD173 pKa = 2.84 LRR175 pKa = 11.84 DD176 pKa = 3.47 RR177 pKa = 11.84 YY178 pKa = 9.28 QVLRR182 pKa = 11.84 KK183 pKa = 8.77 FHH185 pKa = 5.93 ATVVGGPSGMKK196 pKa = 9.33 EE197 pKa = 3.56 QSLVKK202 pKa = 10.4 RR203 pKa = 11.84 FFRR206 pKa = 11.84 VNSHH210 pKa = 4.87 VVYY213 pKa = 10.59 NHH215 pKa = 5.69 QEE217 pKa = 3.45 AAKK220 pKa = 10.4 YY221 pKa = 9.25 EE222 pKa = 4.13 NHH224 pKa = 6.46 TEE226 pKa = 3.99 NALLLYY232 pKa = 7.29 MACTHH237 pKa = 7.07 ASNPVYY243 pKa = 9.86 ATLKK247 pKa = 9.47 IRR249 pKa = 11.84 IYY251 pKa = 10.65 FYY253 pKa = 11.26 DD254 pKa = 3.34 AVTNN258 pKa = 4.16
Molecular weight: 29.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.278
IPC2_protein 9.575
IPC_protein 9.867
Toseland 10.131
ProMoST 9.882
Dawson 10.35
Bjellqvist 10.058
Wikipedia 10.54
Rodwell 10.687
Grimsley 10.438
Solomon 10.379
Lehninger 10.335
Nozaki 10.16
DTASelect 10.043
Thurlkill 10.204
EMBOSS 10.54
Sillero 10.277
Patrickios 10.189
IPC_peptide 10.379
IPC2_peptide 8.946
IPC2.peptide.svr19 8.543
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1633
79
364
204.1
23.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.021 ± 0.589
2.082 ± 0.408
4.96 ± 0.237
4.409 ± 0.558
4.348 ± 0.317
4.838 ± 0.341
3.307 ± 0.464
6.43 ± 0.542
5.818 ± 0.539
6.981 ± 0.479
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.837 ± 0.419
6.369 ± 0.547
5.695 ± 0.441
5.45 ± 0.831
7.287 ± 0.694
8.634 ± 0.742
5.94 ± 0.49
5.205 ± 0.938
1.408 ± 0.189
3.98 ± 0.486
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here