Amino acid dipepetide frequency for Bacillus phage Juan

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.435AlaAla: 0.435 ± 0.225
1.015AlaCys: 1.015 ± 0.355
2.609AlaAsp: 2.609 ± 0.77
3.914AlaGlu: 3.914 ± 1.082
2.609AlaPhe: 2.609 ± 0.723
4.204AlaGly: 4.204 ± 1.005
0.29AlaHis: 0.29 ± 0.209
2.174AlaIle: 2.174 ± 0.509
4.204AlaLys: 4.204 ± 0.756
2.754AlaLeu: 2.754 ± 0.534
1.739AlaMet: 1.739 ± 0.543
2.029AlaAsn: 2.029 ± 0.517
1.449AlaPro: 1.449 ± 0.568
2.464AlaGln: 2.464 ± 0.549
1.449AlaArg: 1.449 ± 0.427
1.884AlaSer: 1.884 ± 0.532
3.334AlaThr: 3.334 ± 0.596
2.754AlaVal: 2.754 ± 0.836
0.725AlaTrp: 0.725 ± 0.335
2.174AlaTyr: 2.174 ± 0.526
0.0AlaXaa: 0.0 ± 0.0
Cys
0.435CysAla: 0.435 ± 0.287
0.145CysCys: 0.145 ± 0.14
1.594CysAsp: 1.594 ± 0.625
1.16CysGlu: 1.16 ± 0.373
0.58CysPhe: 0.58 ± 0.266
0.725CysGly: 0.725 ± 0.464
0.145CysHis: 0.145 ± 0.114
0.435CysIle: 0.435 ± 0.225
1.305CysLys: 1.305 ± 0.53
0.29CysLeu: 0.29 ± 0.199
0.435CysMet: 0.435 ± 0.269
0.435CysAsn: 0.435 ± 0.249
0.725CysPro: 0.725 ± 0.362
0.0CysGln: 0.0 ± 0.0
0.58CysArg: 0.58 ± 0.299
0.29CysSer: 0.29 ± 0.189
0.29CysThr: 0.29 ± 0.28
0.58CysVal: 0.58 ± 0.333
0.29CysTrp: 0.29 ± 0.187
1.015CysTyr: 1.015 ± 0.329
0.0CysXaa: 0.0 ± 0.0
Asp
2.609AspAla: 2.609 ± 0.531
1.305AspCys: 1.305 ± 0.542
3.624AspAsp: 3.624 ± 0.729
5.508AspGlu: 5.508 ± 0.723
4.348AspPhe: 4.348 ± 0.625
5.508AspGly: 5.508 ± 1.422
1.16AspHis: 1.16 ± 0.507
4.928AspIle: 4.928 ± 0.951
5.508AspLys: 5.508 ± 0.512
3.334AspLeu: 3.334 ± 0.781
2.174AspMet: 2.174 ± 0.594
4.783AspAsn: 4.783 ± 0.69
2.899AspPro: 2.899 ± 0.708
2.319AspGln: 2.319 ± 0.562
1.884AspArg: 1.884 ± 0.498
2.174AspSer: 2.174 ± 0.586
3.044AspThr: 3.044 ± 0.83
5.363AspVal: 5.363 ± 0.838
0.435AspTrp: 0.435 ± 0.211
2.609AspTyr: 2.609 ± 0.5
0.0AspXaa: 0.0 ± 0.0
Glu
3.334GluAla: 3.334 ± 0.942
0.87GluCys: 0.87 ± 0.408
4.204GluAsp: 4.204 ± 0.868
6.378GluGlu: 6.378 ± 1.613
4.348GluPhe: 4.348 ± 0.942
4.493GluGly: 4.493 ± 0.686
0.87GluHis: 0.87 ± 0.265
5.798GluIle: 5.798 ± 1.085
6.378GluLys: 6.378 ± 1.598
9.132GluLeu: 9.132 ± 1.266
2.754GluMet: 2.754 ± 0.823
6.523GluAsn: 6.523 ± 1.153
1.015GluPro: 1.015 ± 0.324
3.044GluGln: 3.044 ± 0.74
3.334GluArg: 3.334 ± 0.92
4.493GluSer: 4.493 ± 0.767
4.638GluThr: 4.638 ± 1.19
4.928GluVal: 4.928 ± 0.769
1.16GluTrp: 1.16 ± 0.325
3.769GluTyr: 3.769 ± 0.802
0.0GluXaa: 0.0 ± 0.0
Phe
2.029PheAla: 2.029 ± 0.506
0.435PheCys: 0.435 ± 0.267
4.059PheAsp: 4.059 ± 0.806
3.769PheGlu: 3.769 ± 0.716
1.739PhePhe: 1.739 ± 0.398
1.884PheGly: 1.884 ± 0.712
1.015PheHis: 1.015 ± 0.366
4.928PheIle: 4.928 ± 0.852
4.928PheLys: 4.928 ± 1.034
2.609PheLeu: 2.609 ± 0.687
1.739PheMet: 1.739 ± 0.446
2.319PheAsn: 2.319 ± 0.619
0.87PhePro: 0.87 ± 0.392
0.725PheGln: 0.725 ± 0.293
0.725PheArg: 0.725 ± 0.23
3.769PheSer: 3.769 ± 0.816
2.754PheThr: 2.754 ± 0.672
2.464PheVal: 2.464 ± 0.663
0.435PheTrp: 0.435 ± 0.212
2.319PheTyr: 2.319 ± 0.59
0.0PheXaa: 0.0 ± 0.0
Gly
3.334GlyAla: 3.334 ± 0.729
0.29GlyCys: 0.29 ± 0.213
2.899GlyAsp: 2.899 ± 0.848
5.653GlyGlu: 5.653 ± 0.817
3.769GlyPhe: 3.769 ± 0.573
3.769GlyGly: 3.769 ± 0.963
0.87GlyHis: 0.87 ± 0.408
4.783GlyIle: 4.783 ± 0.823
6.668GlyLys: 6.668 ± 1.466
4.928GlyLeu: 4.928 ± 0.56
2.319GlyMet: 2.319 ± 0.498
4.783GlyAsn: 4.783 ± 1.054
0.435GlyPro: 0.435 ± 0.184
1.594GlyGln: 1.594 ± 0.397
2.174GlyArg: 2.174 ± 0.55
4.493GlySer: 4.493 ± 0.813
5.943GlyThr: 5.943 ± 1.543
3.769GlyVal: 3.769 ± 0.687
0.29GlyTrp: 0.29 ± 0.186
3.044GlyTyr: 3.044 ± 0.686
0.0GlyXaa: 0.0 ± 0.0
His
0.725HisAla: 0.725 ± 0.303
0.145HisCys: 0.145 ± 0.143
0.725HisAsp: 0.725 ± 0.533
0.58HisGlu: 0.58 ± 0.341
1.015HisPhe: 1.015 ± 0.359
0.58HisGly: 0.58 ± 0.381
0.725HisHis: 0.725 ± 0.341
1.449HisIle: 1.449 ± 0.375
1.449HisLys: 1.449 ± 0.383
1.739HisLeu: 1.739 ± 0.544
0.29HisMet: 0.29 ± 0.233
0.58HisAsn: 0.58 ± 0.21
0.145HisPro: 0.145 ± 0.129
0.435HisGln: 0.435 ± 0.254
0.435HisArg: 0.435 ± 0.286
0.58HisSer: 0.58 ± 0.313
1.594HisThr: 1.594 ± 0.392
0.87HisVal: 0.87 ± 0.417
0.145HisTrp: 0.145 ± 0.164
1.16HisTyr: 1.16 ± 0.388
0.0HisXaa: 0.0 ± 0.0
Ile
2.899IleAla: 2.899 ± 0.563
0.435IleCys: 0.435 ± 0.26
6.378IleAsp: 6.378 ± 0.604
6.958IleGlu: 6.958 ± 1.227
1.739IlePhe: 1.739 ± 0.451
3.914IleGly: 3.914 ± 0.682
0.87IleHis: 0.87 ± 0.381
5.073IleIle: 5.073 ± 1.173
6.668IleLys: 6.668 ± 1.159
2.174IleLeu: 2.174 ± 0.677
2.609IleMet: 2.609 ± 0.657
5.218IleAsn: 5.218 ± 0.715
1.739IlePro: 1.739 ± 0.392
2.754IleGln: 2.754 ± 0.574
4.493IleArg: 4.493 ± 0.933
3.334IleSer: 3.334 ± 0.747
4.059IleThr: 4.059 ± 0.781
3.479IleVal: 3.479 ± 0.667
0.725IleTrp: 0.725 ± 0.285
2.464IleTyr: 2.464 ± 0.576
0.0IleXaa: 0.0 ± 0.0
Lys
4.348LysAla: 4.348 ± 1.053
1.16LysCys: 1.16 ± 0.449
6.813LysAsp: 6.813 ± 1.327
9.277LysGlu: 9.277 ± 1.807
3.769LysPhe: 3.769 ± 0.707
7.247LysGly: 7.247 ± 1.119
1.449LysHis: 1.449 ± 0.446
5.508LysIle: 5.508 ± 0.749
10.146LysLys: 10.146 ± 1.239
6.813LysLeu: 6.813 ± 0.989
3.044LysMet: 3.044 ± 0.819
4.493LysAsn: 4.493 ± 0.785
1.015LysPro: 1.015 ± 0.394
3.189LysGln: 3.189 ± 0.667
5.218LysArg: 5.218 ± 1.011
3.479LysSer: 3.479 ± 0.673
5.943LysThr: 5.943 ± 0.93
7.102LysVal: 7.102 ± 0.904
0.87LysTrp: 0.87 ± 0.331
3.914LysTyr: 3.914 ± 0.778
0.0LysXaa: 0.0 ± 0.0
Leu
3.044LeuAla: 3.044 ± 0.658
0.435LeuCys: 0.435 ± 0.219
5.218LeuAsp: 5.218 ± 0.787
5.508LeuGlu: 5.508 ± 1.071
2.319LeuPhe: 2.319 ± 0.711
4.348LeuGly: 4.348 ± 0.903
1.884LeuHis: 1.884 ± 0.508
4.059LeuIle: 4.059 ± 0.866
6.378LeuLys: 6.378 ± 1.124
3.914LeuLeu: 3.914 ± 0.701
2.609LeuMet: 2.609 ± 0.674
4.348LeuAsn: 4.348 ± 0.795
1.739LeuPro: 1.739 ± 0.358
3.044LeuGln: 3.044 ± 0.76
3.189LeuArg: 3.189 ± 0.612
3.624LeuSer: 3.624 ± 0.677
4.638LeuThr: 4.638 ± 0.682
3.334LeuVal: 3.334 ± 0.63
0.87LeuTrp: 0.87 ± 0.371
3.334LeuTyr: 3.334 ± 0.61
0.0LeuXaa: 0.0 ± 0.0
Met
0.725MetAla: 0.725 ± 0.268
0.725MetCys: 0.725 ± 0.306
1.884MetAsp: 1.884 ± 0.508
2.029MetGlu: 2.029 ± 0.465
2.029MetPhe: 2.029 ± 0.643
2.319MetGly: 2.319 ± 0.587
0.145MetHis: 0.145 ± 0.143
1.449MetIle: 1.449 ± 0.586
2.609MetLys: 2.609 ± 0.549
2.899MetLeu: 2.899 ± 0.754
1.594MetMet: 1.594 ± 0.584
3.914MetAsn: 3.914 ± 0.515
0.87MetPro: 0.87 ± 0.252
0.725MetGln: 0.725 ± 0.235
1.739MetArg: 1.739 ± 0.576
2.174MetSer: 2.174 ± 0.521
1.305MetThr: 1.305 ± 0.388
1.015MetVal: 1.015 ± 0.294
1.449MetTrp: 1.449 ± 0.471
1.739MetTyr: 1.739 ± 0.527
0.0MetXaa: 0.0 ± 0.0
Asn
2.319AsnAla: 2.319 ± 0.633
1.015AsnCys: 1.015 ± 0.473
4.783AsnAsp: 4.783 ± 0.745
5.508AsnGlu: 5.508 ± 1.098
3.189AsnPhe: 3.189 ± 0.593
4.928AsnGly: 4.928 ± 0.931
0.58AsnHis: 0.58 ± 0.389
5.073AsnIle: 5.073 ± 0.782
6.378AsnLys: 6.378 ± 1.406
5.073AsnLeu: 5.073 ± 0.842
1.305AsnMet: 1.305 ± 0.557
6.233AsnAsn: 6.233 ± 1.143
2.464AsnPro: 2.464 ± 0.711
2.464AsnGln: 2.464 ± 0.562
2.754AsnArg: 2.754 ± 0.791
4.493AsnSer: 4.493 ± 0.708
3.769AsnThr: 3.769 ± 1.043
4.493AsnVal: 4.493 ± 0.653
1.015AsnTrp: 1.015 ± 0.312
3.334AsnTyr: 3.334 ± 0.984
0.0AsnXaa: 0.0 ± 0.0
Pro
1.015ProAla: 1.015 ± 0.387
0.29ProCys: 0.29 ± 0.192
1.884ProAsp: 1.884 ± 0.55
1.449ProGlu: 1.449 ± 0.456
1.015ProPhe: 1.015 ± 0.528
0.58ProGly: 0.58 ± 0.24
0.145ProHis: 0.145 ± 0.162
1.594ProIle: 1.594 ± 0.487
2.464ProLys: 2.464 ± 0.636
1.305ProLeu: 1.305 ± 0.403
1.305ProMet: 1.305 ± 0.559
1.594ProAsn: 1.594 ± 0.624
1.015ProPro: 1.015 ± 0.374
0.58ProGln: 0.58 ± 0.259
0.725ProArg: 0.725 ± 0.396
2.029ProSer: 2.029 ± 0.538
1.884ProThr: 1.884 ± 0.555
1.739ProVal: 1.739 ± 0.423
0.29ProTrp: 0.29 ± 0.204
1.449ProTyr: 1.449 ± 0.649
0.0ProXaa: 0.0 ± 0.0
Gln
1.015GlnAla: 1.015 ± 0.375
0.145GlnCys: 0.145 ± 0.114
1.449GlnAsp: 1.449 ± 0.559
1.739GlnGlu: 1.739 ± 0.596
1.305GlnPhe: 1.305 ± 0.478
2.319GlnGly: 2.319 ± 0.555
0.145GlnHis: 0.145 ± 0.114
2.029GlnIle: 2.029 ± 0.491
2.899GlnLys: 2.899 ± 0.64
4.348GlnLeu: 4.348 ± 0.707
0.58GlnMet: 0.58 ± 0.249
2.899GlnAsn: 2.899 ± 0.61
1.015GlnPro: 1.015 ± 0.399
0.87GlnGln: 0.87 ± 0.319
1.16GlnArg: 1.16 ± 0.361
2.029GlnSer: 2.029 ± 0.678
2.464GlnThr: 2.464 ± 0.534
2.029GlnVal: 2.029 ± 0.598
0.29GlnTrp: 0.29 ± 0.202
2.609GlnTyr: 2.609 ± 0.666
0.0GlnXaa: 0.0 ± 0.0
Arg
3.044ArgAla: 3.044 ± 0.492
0.435ArgCys: 0.435 ± 0.213
2.029ArgAsp: 2.029 ± 0.544
3.189ArgGlu: 3.189 ± 0.704
2.319ArgPhe: 2.319 ± 0.596
2.174ArgGly: 2.174 ± 0.614
0.29ArgHis: 0.29 ± 0.161
2.464ArgIle: 2.464 ± 0.693
4.928ArgLys: 4.928 ± 1.092
2.319ArgLeu: 2.319 ± 0.669
1.16ArgMet: 1.16 ± 0.503
2.754ArgAsn: 2.754 ± 0.73
0.725ArgPro: 0.725 ± 0.311
1.449ArgGln: 1.449 ± 0.476
2.174ArgArg: 2.174 ± 0.605
2.174ArgSer: 2.174 ± 0.706
2.029ArgThr: 2.029 ± 0.579
2.029ArgVal: 2.029 ± 0.642
0.29ArgTrp: 0.29 ± 0.165
2.319ArgTyr: 2.319 ± 0.809
0.0ArgXaa: 0.0 ± 0.0
Ser
2.899SerAla: 2.899 ± 0.503
0.725SerCys: 0.725 ± 0.299
3.624SerAsp: 3.624 ± 0.696
3.624SerGlu: 3.624 ± 0.842
2.319SerPhe: 2.319 ± 0.609
2.899SerGly: 2.899 ± 0.573
1.305SerHis: 1.305 ± 0.388
3.334SerIle: 3.334 ± 0.794
4.204SerLys: 4.204 ± 0.927
3.334SerLeu: 3.334 ± 0.685
2.029SerMet: 2.029 ± 0.559
4.783SerAsn: 4.783 ± 0.828
0.725SerPro: 0.725 ± 0.23
2.174SerGln: 2.174 ± 0.549
1.884SerArg: 1.884 ± 0.512
2.319SerSer: 2.319 ± 0.739
3.479SerThr: 3.479 ± 0.769
3.479SerVal: 3.479 ± 0.725
0.145SerTrp: 0.145 ± 0.164
3.189SerTyr: 3.189 ± 0.52
0.0SerXaa: 0.0 ± 0.0
Thr
3.334ThrAla: 3.334 ± 0.931
0.58ThrCys: 0.58 ± 0.289
3.044ThrAsp: 3.044 ± 0.746
4.204ThrGlu: 4.204 ± 0.647
2.464ThrPhe: 2.464 ± 0.457
5.218ThrGly: 5.218 ± 1.333
1.16ThrHis: 1.16 ± 0.422
5.218ThrIle: 5.218 ± 0.731
5.943ThrLys: 5.943 ± 0.862
5.073ThrLeu: 5.073 ± 1.059
1.739ThrMet: 1.739 ± 0.384
4.204ThrAsn: 4.204 ± 0.805
1.739ThrPro: 1.739 ± 0.544
1.449ThrGln: 1.449 ± 0.378
2.464ThrArg: 2.464 ± 0.549
4.059ThrSer: 4.059 ± 0.799
5.218ThrThr: 5.218 ± 1.762
4.493ThrVal: 4.493 ± 0.742
0.29ThrTrp: 0.29 ± 0.195
1.594ThrTyr: 1.594 ± 0.518
0.0ThrXaa: 0.0 ± 0.0
Val
2.899ValAla: 2.899 ± 0.793
0.145ValCys: 0.145 ± 0.159
5.073ValAsp: 5.073 ± 1.05
5.653ValGlu: 5.653 ± 1.066
1.594ValPhe: 1.594 ± 0.419
5.073ValGly: 5.073 ± 0.66
1.16ValHis: 1.16 ± 0.324
3.914ValIle: 3.914 ± 0.837
6.378ValLys: 6.378 ± 0.866
2.754ValLeu: 2.754 ± 0.525
1.594ValMet: 1.594 ± 0.403
4.059ValAsn: 4.059 ± 0.938
2.464ValPro: 2.464 ± 0.749
2.174ValGln: 2.174 ± 0.519
1.884ValArg: 1.884 ± 0.452
3.189ValSer: 3.189 ± 0.795
4.348ValThr: 4.348 ± 0.878
4.204ValVal: 4.204 ± 1.008
1.015ValTrp: 1.015 ± 0.424
2.754ValTyr: 2.754 ± 0.621
0.0ValXaa: 0.0 ± 0.0
Trp
0.29TrpAla: 0.29 ± 0.186
0.145TrpCys: 0.145 ± 0.129
0.435TrpAsp: 0.435 ± 0.226
0.725TrpGlu: 0.725 ± 0.319
1.305TrpPhe: 1.305 ± 0.414
0.58TrpGly: 0.58 ± 0.28
0.58TrpHis: 0.58 ± 0.211
0.435TrpIle: 0.435 ± 0.253
1.16TrpLys: 1.16 ± 0.412
0.725TrpLeu: 0.725 ± 0.356
0.58TrpMet: 0.58 ± 0.247
1.594TrpAsn: 1.594 ± 0.399
0.0TrpPro: 0.0 ± 0.0
0.725TrpGln: 0.725 ± 0.261
0.58TrpArg: 0.58 ± 0.252
0.29TrpSer: 0.29 ± 0.205
0.145TrpThr: 0.145 ± 0.14
0.725TrpVal: 0.725 ± 0.296
0.29TrpTrp: 0.29 ± 0.312
0.58TrpTyr: 0.58 ± 0.283
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.479TyrAla: 3.479 ± 0.552
1.015TyrCys: 1.015 ± 0.402
3.189TyrAsp: 3.189 ± 0.676
4.638TyrGlu: 4.638 ± 0.857
1.739TyrPhe: 1.739 ± 0.439
3.044TyrGly: 3.044 ± 0.624
0.58TyrHis: 0.58 ± 0.268
3.334TyrIle: 3.334 ± 0.722
4.204TyrLys: 4.204 ± 0.758
2.319TyrLeu: 2.319 ± 0.566
1.594TyrMet: 1.594 ± 0.612
3.624TyrAsn: 3.624 ± 0.554
1.305TyrPro: 1.305 ± 0.328
1.16TyrGln: 1.16 ± 0.329
1.594TyrArg: 1.594 ± 0.362
1.594TyrSer: 1.594 ± 0.497
2.609TyrThr: 2.609 ± 0.484
3.479TyrVal: 3.479 ± 0.543
0.87TyrTrp: 0.87 ± 0.474
2.029TyrTyr: 2.029 ± 0.516
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 34 proteins (6900 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski