Candidatus Dependentiae bacterium (ex Spumella elongata CCAP 955/1)

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Candidatus Dependentiae; unclassified Candidatus Dependentiae

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1075 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345ZAF0|A0A345ZAF0_9BACT AmmeMemoRadiSam system protein B OS=Candidatus Dependentiae bacterium (ex Spumella elongata CCAP 955/1) OX=2066483 GN=amrB PE=3 SV=1
MM1 pKa = 5.91QTKK4 pKa = 10.84LILNLIACVSFVDD17 pKa = 5.64TIFTSQNAEE26 pKa = 3.7IEE28 pKa = 4.27LAEE31 pKa = 4.22SFQTHH36 pKa = 5.71GLVARR41 pKa = 11.84VSSKK45 pKa = 11.17KK46 pKa = 10.41LFQDD50 pKa = 3.09VDD52 pKa = 4.16SQCEE56 pKa = 3.81DD57 pKa = 3.44DD58 pKa = 5.26LEE60 pKa = 5.24NIFISRR66 pKa = 11.84EE67 pKa = 3.65AVKK70 pKa = 10.12IYY72 pKa = 10.23FYY74 pKa = 11.06LQDD77 pKa = 3.79LDD79 pKa = 4.39EE80 pKa = 4.84VEE82 pKa = 4.73NCHH85 pKa = 6.42SCTSEE90 pKa = 3.98SLDD93 pKa = 4.31VIEE96 pKa = 5.9DD97 pKa = 3.78YY98 pKa = 11.44CLVDD102 pKa = 3.26TDD104 pKa = 4.94EE105 pKa = 4.39ITMNASDD112 pKa = 4.19HH113 pKa = 6.06GQDD116 pKa = 3.56FFNQKK121 pKa = 10.16VITGDD126 pKa = 3.68DD127 pKa = 3.78MYY129 pKa = 11.22TSLGLSYY136 pKa = 10.95FDD138 pKa = 4.15GEE140 pKa = 4.67KK141 pKa = 10.43EE142 pKa = 4.1VEE144 pKa = 4.04

Molecular weight: 16.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345ZCR8|A0A345ZCR8_9BACT Elongation factor Ts OS=Candidatus Dependentiae bacterium (ex Spumella elongata CCAP 955/1) OX=2066483 GN=tsf PE=3 SV=1
MM1 pKa = 7.41SVTFFKK7 pKa = 10.76KK8 pKa = 10.49SRR10 pKa = 11.84VKK12 pKa = 10.55RR13 pKa = 11.84NRR15 pKa = 11.84KK16 pKa = 8.43HH17 pKa = 5.92GFRR20 pKa = 11.84KK21 pKa = 10.02RR22 pKa = 11.84MSTLSGRR29 pKa = 11.84TIINRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.64GRR40 pKa = 11.84KK41 pKa = 8.55RR42 pKa = 11.84LAVRR46 pKa = 11.84AA47 pKa = 4.0

Molecular weight: 5.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1075

0

1075

327330

38

2248

304.5

34.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.466 ± 0.097

1.393 ± 0.034

5.536 ± 0.059

5.52 ± 0.085

4.756 ± 0.072

5.171 ± 0.089

2.432 ± 0.043

8.057 ± 0.075

7.148 ± 0.091

9.778 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.635 ± 0.038

5.358 ± 0.068

3.185 ± 0.041

5.296 ± 0.066

3.144 ± 0.054

6.845 ± 0.068

5.685 ± 0.099

6.064 ± 0.072

0.795 ± 0.025

3.736 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski