Chloebia gouldiae (Gouldian finch) (Erythrura gouldiae)
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 18274 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3L8Q6N5|A0A3L8Q6N5_CHLGU Uncharacterized protein OS=Chloebia gouldiae OX=44316 GN=DV515_00018918 PE=4 SV=1
MM1 pKa = 7.32 FLQHH5 pKa = 6.99 PSRR8 pKa = 11.84 IRR10 pKa = 11.84 HH11 pKa = 4.53 STSPEE16 pKa = 3.72 GFSCWKK22 pKa = 10.66 AVTTCPAAASSSWGAGTEE40 pKa = 4.13 SSSWGPRR47 pKa = 11.84 CAAAAGDD54 pKa = 4.33 SDD56 pKa = 4.91 PSISDD61 pKa = 3.61 PAFSASSAPGAQSPDD76 pKa = 3.12 DD77 pKa = 3.98 SAPGNSVPGDD87 pKa = 3.85 SAPGHH92 pKa = 6.27 AALDD96 pKa = 3.86 NSASAVSYY104 pKa = 9.32 PAFSASSAPGAEE116 pKa = 4.37 SPDD119 pKa = 3.72 DD120 pKa = 3.95 SAASLPDD127 pKa = 3.28 NSAPDD132 pKa = 3.28 NSVPNNSVPGNSASSALSYY151 pKa = 10.83 LAFSASSAPGALSPDD166 pKa = 3.23 APVFSASSAHH176 pKa = 6.41 GNSAPDD182 pKa = 4.11 DD183 pKa = 4.2 SAASPPPDD191 pKa = 3.53 SASPSPDD198 pKa = 3.51 LILFLTLQLFLLPLLLMLHH217 pKa = 6.27 LQMLLLLLLQLFLLPLLL234 pKa = 4.46
Molecular weight: 23.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.897
IPC_protein 3.897
Toseland 3.668
ProMoST 4.101
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.91
Rodwell 3.732
Grimsley 3.579
Solomon 3.91
Lehninger 3.872
Nozaki 4.05
DTASelect 4.355
Thurlkill 3.745
EMBOSS 3.91
Sillero 4.037
Patrickios 1.952
IPC_peptide 3.91
IPC2_peptide 4.012
IPC2.peptide.svr19 3.924
Protein with the highest isoelectric point:
>tr|A0A3L8R0J0|A0A3L8R0J0_CHLGU Uncharacterized protein (Fragment) OS=Chloebia gouldiae OX=44316 GN=DV515_00017241 PE=3 SV=1
MM1 pKa = 7.68 GGPGAGGLGLGLGLRR16 pKa = 11.84 FGSVLALGAQVWLWGLRR33 pKa = 11.84 FGSVLGLGLRR43 pKa = 11.84 FGSVLGLGLRR53 pKa = 11.84 FGSVLALGAQVWLRR67 pKa = 11.84 FGAGAQVWLRR77 pKa = 11.84 FGSGGSGLAPFWGWGLRR94 pKa = 11.84 FGSILGLGLRR104 pKa = 11.84 FGSVLRR110 pKa = 11.84 LGLRR114 pKa = 11.84 FGSVLGLGLRR124 pKa = 11.84 FGAGGSGLAPFWGWGLRR141 pKa = 11.84 FGSVLGLGAQQ151 pKa = 3.43
Molecular weight: 15.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.544
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.281
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.236
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18273
1
18274
8488823
49
7429
464.5
51.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.199 ± 0.022
2.322 ± 0.017
4.742 ± 0.012
7.084 ± 0.027
3.617 ± 0.013
6.76 ± 0.028
2.546 ± 0.008
4.356 ± 0.016
5.77 ± 0.025
9.787 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.181 ± 0.007
3.603 ± 0.013
6.164 ± 0.028
4.731 ± 0.017
5.694 ± 0.019
8.335 ± 0.025
5.119 ± 0.015
6.087 ± 0.017
1.318 ± 0.008
2.58 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here