Ecklonia radiata-associated virus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2CHS8|A0A3G2CHS8_9CIRC Replication-associated protein OS=Ecklonia radiata-associated virus 1 OX=2480191 PE=3 SV=1
MM1 pKa = 7.26TRR3 pKa = 11.84RR4 pKa = 11.84NHH6 pKa = 6.25HH7 pKa = 6.23AVKK10 pKa = 10.26AWFLTISQVAEE21 pKa = 4.05GEE23 pKa = 4.24NNQKK27 pKa = 10.42LKK29 pKa = 11.26ALIEE33 pKa = 4.1EE34 pKa = 4.64KK35 pKa = 10.59ALSVHH40 pKa = 7.0DD41 pKa = 4.15EE42 pKa = 4.0VLEE45 pKa = 3.83YY46 pKa = 10.61VYY48 pKa = 10.95CNEE51 pKa = 3.53LHH53 pKa = 6.91ADD55 pKa = 3.62NGRR58 pKa = 11.84HH59 pKa = 3.15VHH61 pKa = 6.84AYY63 pKa = 8.43VKK65 pKa = 10.12LRR67 pKa = 11.84KK68 pKa = 9.51GVTHH72 pKa = 7.15ASAPGFFHH80 pKa = 7.03VFHH83 pKa = 6.54NTANCEE89 pKa = 4.04PARR92 pKa = 11.84SAQAVIKK99 pKa = 10.05YY100 pKa = 8.32CAKK103 pKa = 10.18EE104 pKa = 3.46GDD106 pKa = 4.51YY107 pKa = 9.74ITNIPQKK114 pKa = 7.52VTRR117 pKa = 11.84KK118 pKa = 9.76KK119 pKa = 8.74RR120 pKa = 11.84TKK122 pKa = 9.55VSYY125 pKa = 10.86DD126 pKa = 3.15VVLNKK131 pKa = 8.17TTEE134 pKa = 3.96EE135 pKa = 4.08AFSEE139 pKa = 4.98GIISFTQVTAYY150 pKa = 9.9QRR152 pKa = 11.84ARR154 pKa = 11.84RR155 pKa = 11.84LATLSQTYY163 pKa = 9.77NHH165 pKa = 7.1DD166 pKa = 3.56GVRR169 pKa = 11.84GLWFYY174 pKa = 11.4GPSGTGKK181 pKa = 10.02SRR183 pKa = 11.84SARR186 pKa = 11.84HH187 pKa = 5.41SSLIEE192 pKa = 3.68GKK194 pKa = 10.49RR195 pKa = 11.84FLKK198 pKa = 10.02PQNKK202 pKa = 7.99WWDD205 pKa = 3.84GYY207 pKa = 10.51AGEE210 pKa = 5.12PIVVLDD216 pKa = 5.41DD217 pKa = 4.79LDD219 pKa = 4.03TDD221 pKa = 4.14VLGHH225 pKa = 6.39HH226 pKa = 6.72LKK228 pKa = 9.89IWSDD232 pKa = 3.57RR233 pKa = 11.84YY234 pKa = 10.8ACTGEE239 pKa = 4.58TKK241 pKa = 10.77GSTCNLLHH249 pKa = 6.47KK250 pKa = 10.47QFIVTSNYY258 pKa = 10.31SIEE261 pKa = 4.22EE262 pKa = 4.21LFQKK266 pKa = 9.49STMNIEE272 pKa = 3.64PLKK275 pKa = 10.65RR276 pKa = 11.84RR277 pKa = 11.84FKK279 pKa = 10.42EE280 pKa = 4.24VYY282 pKa = 9.93FPEE285 pKa = 4.56STSNQQPNLYY295 pKa = 8.09QTSAFLSNQPXDD307 pKa = 3.44

Molecular weight: 34.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2CHS8|A0A3G2CHS8_9CIRC Replication-associated protein OS=Ecklonia radiata-associated virus 1 OX=2480191 PE=3 SV=1
MM1 pKa = 7.26TRR3 pKa = 11.84RR4 pKa = 11.84NHH6 pKa = 6.25HH7 pKa = 6.23AVKK10 pKa = 10.26AWFLTISQVAEE21 pKa = 4.05GEE23 pKa = 4.24NNQKK27 pKa = 10.42LKK29 pKa = 11.26ALIEE33 pKa = 4.1EE34 pKa = 4.64KK35 pKa = 10.59ALSVHH40 pKa = 7.0DD41 pKa = 4.15EE42 pKa = 4.0VLEE45 pKa = 3.83YY46 pKa = 10.61VYY48 pKa = 10.95CNEE51 pKa = 3.53LHH53 pKa = 6.91ADD55 pKa = 3.62NGRR58 pKa = 11.84HH59 pKa = 3.15VHH61 pKa = 6.84AYY63 pKa = 8.43VKK65 pKa = 10.12LRR67 pKa = 11.84KK68 pKa = 9.51GVTHH72 pKa = 7.15ASAPGFFHH80 pKa = 7.03VFHH83 pKa = 6.54NTANCEE89 pKa = 4.04PARR92 pKa = 11.84SAQAVIKK99 pKa = 10.05YY100 pKa = 8.32CAKK103 pKa = 10.18EE104 pKa = 3.46GDD106 pKa = 4.51YY107 pKa = 9.74ITNIPQKK114 pKa = 7.52VTRR117 pKa = 11.84KK118 pKa = 9.76KK119 pKa = 8.74RR120 pKa = 11.84TKK122 pKa = 9.55VSYY125 pKa = 10.86DD126 pKa = 3.15VVLNKK131 pKa = 8.17TTEE134 pKa = 3.96EE135 pKa = 4.08AFSEE139 pKa = 4.98GIISFTQVTAYY150 pKa = 9.9QRR152 pKa = 11.84ARR154 pKa = 11.84RR155 pKa = 11.84LATLSQTYY163 pKa = 9.77NHH165 pKa = 7.1DD166 pKa = 3.56GVRR169 pKa = 11.84GLWFYY174 pKa = 11.4GPSGTGKK181 pKa = 10.02SRR183 pKa = 11.84SARR186 pKa = 11.84HH187 pKa = 5.41SSLIEE192 pKa = 3.68GKK194 pKa = 10.49RR195 pKa = 11.84FLKK198 pKa = 10.02PQNKK202 pKa = 7.99WWDD205 pKa = 3.84GYY207 pKa = 10.51AGEE210 pKa = 5.12PIVVLDD216 pKa = 5.41DD217 pKa = 4.79LDD219 pKa = 4.03TDD221 pKa = 4.14VLGHH225 pKa = 6.39HH226 pKa = 6.72LKK228 pKa = 9.89IWSDD232 pKa = 3.57RR233 pKa = 11.84YY234 pKa = 10.8ACTGEE239 pKa = 4.58TKK241 pKa = 10.77GSTCNLLHH249 pKa = 6.47KK250 pKa = 10.47QFIVTSNYY258 pKa = 10.31SIEE261 pKa = 4.22EE262 pKa = 4.21LFQKK266 pKa = 9.49STMNIEE272 pKa = 3.64PLKK275 pKa = 10.65RR276 pKa = 11.84RR277 pKa = 11.84FKK279 pKa = 10.42EE280 pKa = 4.24VYY282 pKa = 9.93FPEE285 pKa = 4.56STSNQQPNLYY295 pKa = 8.09QTSAFLSNQPXDD307 pKa = 3.44

Molecular weight: 34.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

307

307

307

307.0

34.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.166 ± 0.0

1.629 ± 0.0

3.909 ± 0.0

6.515 ± 0.0

4.235 ± 0.0

5.537 ± 0.0

4.56 ± 0.0

4.235 ± 0.0

7.492 ± 0.0

7.492 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.651 ± 0.0

5.537 ± 0.0

3.257 ± 0.0

4.56 ± 0.0

5.537 ± 0.0

7.166 ± 0.0

7.166 ± 0.0

6.84 ± 0.0

1.629 ± 0.0

4.56 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski