Coleofasciculus chthonoplastes PCC 7420

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Coleofasciculaceae; Coleofasciculus; Coleofasciculus chthonoplastes

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8193 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B4VNS3|B4VNS3_9CYAN Uncharacterized protein OS=Coleofasciculus chthonoplastes PCC 7420 OX=118168 GN=MC7420_4582 PE=4 SV=1
MM1 pKa = 7.9KK2 pKa = 9.03ITAIATGFSLLSLLYY17 pKa = 9.24PLSAEE22 pKa = 4.58AIQFNQIYY30 pKa = 9.64VFSDD34 pKa = 3.39SSADD38 pKa = 3.4PGNVFNVTSAANSSGLPVPITPSPPYY64 pKa = 10.36FEE66 pKa = 5.79GRR68 pKa = 11.84FSNGYY73 pKa = 8.47NWIDD77 pKa = 3.23ILTSEE82 pKa = 5.02LGVDD86 pKa = 5.17LIPSTEE92 pKa = 4.08LTVGEE97 pKa = 4.86PIIPSIPPAVNFNFGGATATNSVNFAFGGAQTGLGNVFPEE137 pKa = 4.47LGVGVLTEE145 pKa = 3.99INGFINDD152 pKa = 4.03LNVAQTSANPDD163 pKa = 2.93ALYY166 pKa = 10.31IVWAGNNDD174 pKa = 3.53YY175 pKa = 11.39SKK177 pKa = 11.52GGLRR181 pKa = 11.84DD182 pKa = 3.31STEE185 pKa = 4.42PINNLFTGVTALADD199 pKa = 3.52AGARR203 pKa = 11.84NFFIPNLPDD212 pKa = 3.41LGKK215 pKa = 8.55TPRR218 pKa = 11.84AIIQGEE224 pKa = 4.15AAEE227 pKa = 4.28LTQVSAEE234 pKa = 4.19HH235 pKa = 6.14NAGLTTMFDD244 pKa = 4.38DD245 pKa = 6.04LRR247 pKa = 11.84LTYY250 pKa = 10.92DD251 pKa = 5.04DD252 pKa = 4.49INIIPFDD259 pKa = 3.97LASLFQEE266 pKa = 5.44VITQPDD272 pKa = 3.38EE273 pKa = 4.44FGFTNVTEE281 pKa = 4.26PCFTEE286 pKa = 5.74LGVCSNPDD294 pKa = 3.29EE295 pKa = 4.15YY296 pKa = 11.68LFWDD300 pKa = 5.48DD301 pKa = 4.16IHH303 pKa = 6.68PTAQGHH309 pKa = 5.87ALWGDD314 pKa = 3.42YY315 pKa = 10.59AYY317 pKa = 10.65AALQDD322 pKa = 3.88AGVEE326 pKa = 4.17PKK328 pKa = 10.29SVPEE332 pKa = 3.89PAFSIPLLLMGIGFLLQSTVRR353 pKa = 11.84YY354 pKa = 9.81RR355 pKa = 11.84NSLL358 pKa = 3.43

Molecular weight: 38.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B4VMV6|B4VMV6_9CYAN Protein translocase subunit SecD OS=Coleofasciculus chthonoplastes PCC 7420 OX=118168 GN=secD PE=3 SV=1
MM1 pKa = 7.44RR2 pKa = 11.84RR3 pKa = 11.84AGFVFPLSVRR13 pKa = 11.84RR14 pKa = 11.84AGFVFPLSVRR24 pKa = 11.84RR25 pKa = 11.84AGFVFHH31 pKa = 6.85LSVRR35 pKa = 11.84RR36 pKa = 11.84AGFVFHH42 pKa = 6.85LSVRR46 pKa = 11.84RR47 pKa = 11.84AGFVFPLSVRR57 pKa = 11.84RR58 pKa = 11.84AGFVFPLSVRR68 pKa = 11.84RR69 pKa = 11.84AGFVFPLSVTSS80 pKa = 3.76

Molecular weight: 9.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8193

0

8193

2324273

29

4083

283.7

31.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.797 ± 0.03

1.029 ± 0.01

5.241 ± 0.029

6.256 ± 0.037

3.821 ± 0.02

6.768 ± 0.048

1.906 ± 0.015

6.413 ± 0.024

4.338 ± 0.031

10.942 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.821 ± 0.013

4.276 ± 0.037

4.991 ± 0.028

5.853 ± 0.031

5.386 ± 0.028

6.506 ± 0.03

5.827 ± 0.03

6.371 ± 0.026

1.479 ± 0.013

2.966 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski