Sutterella wadsworthensis 2_1_59BFAA
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2327 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K1KID3|K1KID3_9BURK Uncharacterized protein OS=Sutterella wadsworthensis 2_1_59BFAA OX=742823 GN=HMPREF9465_00864 PE=4 SV=1
MM1 pKa = 7.54 TISEE5 pKa = 5.04 LIEE8 pKa = 3.83 LLQEE12 pKa = 3.96 IMEE15 pKa = 4.38 KK16 pKa = 10.7 EE17 pKa = 3.72 GDD19 pKa = 3.41 IEE21 pKa = 4.61 VAYY24 pKa = 9.17 TYY26 pKa = 11.39 NDD28 pKa = 2.73 GGYY31 pKa = 9.69 PMMGEE36 pKa = 4.59 EE37 pKa = 4.17 YY38 pKa = 10.64 AGGVEE43 pKa = 4.94 VRR45 pKa = 11.84 LTPYY49 pKa = 10.56 GKK51 pKa = 10.27 AVVIWW56 pKa = 3.62
Molecular weight: 6.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.878
IPC2_protein 4.101
IPC_protein 3.884
Toseland 3.757
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.706
Rodwell 3.745
Grimsley 3.681
Solomon 3.795
Lehninger 3.745
Nozaki 3.973
DTASelect 3.999
Thurlkill 3.783
EMBOSS 3.719
Sillero 3.999
Patrickios 0.604
IPC_peptide 3.808
IPC2_peptide 3.986
IPC2.peptide.svr19 3.911
Protein with the highest isoelectric point:
>tr|K1JFF1|K1JFF1_9BURK Uncharacterized protein (Fragment) OS=Sutterella wadsworthensis 2_1_59BFAA OX=742823 GN=HMPREF9465_02131 PE=4 SV=1
MM1 pKa = 8.02 RR2 pKa = 11.84 DD3 pKa = 3.18 TRR5 pKa = 11.84 KK6 pKa = 9.85 IEE8 pKa = 4.05 ADD10 pKa = 3.48 VNNPRR15 pKa = 11.84 LRR17 pKa = 11.84 RR18 pKa = 11.84 MFPRR22 pKa = 11.84 GNSMAALLKK31 pKa = 10.87 LLLFTLLMHH40 pKa = 6.46 TACTSLRR47 pKa = 11.84 FTATLDD53 pKa = 4.25 AIHH56 pKa = 7.0 AGASSFEE63 pKa = 4.19 VGLMLASISFGPMFLAIAAGRR84 pKa = 11.84 WLDD87 pKa = 3.46 RR88 pKa = 11.84 SGPRR92 pKa = 11.84 APMLASRR99 pKa = 11.84 LSVMAAAGCAILLPASDD116 pKa = 5.16 LGIAPLFGACVLTGLSFMLTNVVIQRR142 pKa = 11.84 LTGDD146 pKa = 3.08 VTTPKK151 pKa = 10.15 NRR153 pKa = 11.84 RR154 pKa = 11.84 FAFTALSLTTASSNLAAPVVAGYY177 pKa = 10.38 VIEE180 pKa = 4.33 RR181 pKa = 11.84 LSYY184 pKa = 7.78 TAVYY188 pKa = 8.96 VWSAVAPLILSVLLFVPAFGIVMKK212 pKa = 10.58 ARR214 pKa = 11.84 PRR216 pKa = 11.84 AVRR219 pKa = 11.84 AEE221 pKa = 3.85 AGRR224 pKa = 11.84 TKK226 pKa = 10.85 GSAADD231 pKa = 3.85 FLRR234 pKa = 11.84 DD235 pKa = 3.3 GPMRR239 pKa = 11.84 AVLVASVIISIAWEE253 pKa = 4.06 VGNLLIPIYY262 pKa = 10.42 AAGVGLTPSEE272 pKa = 4.25 IGWVLGSFAAATFIVRR288 pKa = 11.84 FVTPFLLKK296 pKa = 10.6 VVLEE300 pKa = 3.65 WHH302 pKa = 6.59 MIVLSLLLATAAFAVMPLVTTQGAMMGAAFVLGLGLGASLPNMMSLVYY350 pKa = 10.39 LFSPPEE356 pKa = 4.38 RR357 pKa = 11.84 IGEE360 pKa = 4.38 AIGLRR365 pKa = 11.84 IMALNLGKK373 pKa = 10.37 SVFPVLAGLLGNWIGAGSTIWCLAAFAASGCGYY406 pKa = 10.53 AGAAARR412 pKa = 11.84 TVLSRR417 pKa = 11.84 MKK419 pKa = 10.51 ALRR422 pKa = 11.84 DD423 pKa = 3.66 AKK425 pKa = 10.56 IDD427 pKa = 3.6 GG428 pKa = 3.95
Molecular weight: 45.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.575
IPC_protein 10.379
Toseland 10.511
ProMoST 10.248
Dawson 10.643
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 10.804
Grimsley 10.701
Solomon 10.745
Lehninger 10.701
Nozaki 10.526
DTASelect 10.365
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.584
Patrickios 10.482
IPC_peptide 10.745
IPC2_peptide 9.56
IPC2.peptide.svr19 8.545
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2327
0
2327
737528
29
1908
316.9
34.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.211 ± 0.069
1.32 ± 0.022
5.463 ± 0.039
6.541 ± 0.052
3.86 ± 0.041
7.976 ± 0.053
1.932 ± 0.022
5.003 ± 0.035
4.435 ± 0.053
9.661 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.779 ± 0.022
2.99 ± 0.036
4.581 ± 0.037
2.795 ± 0.026
6.848 ± 0.072
5.584 ± 0.039
5.595 ± 0.038
7.787 ± 0.045
1.3 ± 0.02
2.339 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here