Pseudomonas phage PPpW-3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5YUN9|V5YUN9_9CAUD Probable tape measure protein OS=Pseudomonas phage PPpW-3 OX=1279082 PE=3 SV=1
MM1 pKa = 7.85IYY3 pKa = 10.76AQFKK7 pKa = 9.11DD8 pKa = 3.59GKK10 pKa = 9.12IISVFPSPQDD20 pKa = 3.32PAHH23 pKa = 6.35WPGIEE28 pKa = 4.59EE29 pKa = 4.28IAADD33 pKa = 3.94DD34 pKa = 4.23PRR36 pKa = 11.84YY37 pKa = 9.42LAYY40 pKa = 10.75LEE42 pKa = 5.16TITEE46 pKa = 4.14PQVIASEE53 pKa = 4.44DD54 pKa = 3.25GGANVV59 pKa = 4.2

Molecular weight: 6.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5YSW3|V5YSW3_9CAUD Uncharacterized protein OS=Pseudomonas phage PPpW-3 OX=1279082 PE=4 SV=1
MM1 pKa = 7.2NVSSWKK7 pKa = 8.42PQPVTLYY14 pKa = 10.28DD15 pKa = 3.49GRR17 pKa = 11.84VVMSDD22 pKa = 3.01SADD25 pKa = 3.21WLHH28 pKa = 6.0EE29 pKa = 4.09CEE31 pKa = 4.36ARR33 pKa = 11.84SIIALPGTIARR44 pKa = 11.84RR45 pKa = 11.84ARR47 pKa = 11.84LRR49 pKa = 11.84GRR51 pKa = 11.84LDD53 pKa = 3.13EE54 pKa = 4.53SGRR57 pKa = 11.84ISGGILQKK65 pKa = 10.65RR66 pKa = 11.84GLAAVEE72 pKa = 3.81RR73 pKa = 11.84LEE75 pKa = 4.32RR76 pKa = 11.84TIKK79 pKa = 10.02QIWYY83 pKa = 9.87ARR85 pKa = 3.62

Molecular weight: 9.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

13588

51

759

205.9

22.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.938 ± 0.493

1.082 ± 0.145

5.924 ± 0.233

5.991 ± 0.306

2.87 ± 0.177

8.53 ± 0.311

1.766 ± 0.141

4.578 ± 0.194

4.96 ± 0.25

7.978 ± 0.236

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.701 ± 0.146

3.474 ± 0.198

4.151 ± 0.225

4.305 ± 0.249

6.697 ± 0.308

5.21 ± 0.222

5.829 ± 0.355

6.962 ± 0.284

1.626 ± 0.14

2.429 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski