Pseudoplusia includens SNPV IE
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 141 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4ZUP2|A0A0B4ZUP2_9ABAC Uncharacterized protein OS=Pseudoplusia includens SNPV IE OX=1592335 GN=ORF-111 PE=4 SV=1
MM1 pKa = 7.08 FVSTEE6 pKa = 3.92 EE7 pKa = 3.97 VSRR10 pKa = 11.84 GGLANIINMFAYY22 pKa = 9.65 KK23 pKa = 10.41 FIDD26 pKa = 3.26 SDD28 pKa = 4.23 YY29 pKa = 9.9 EE30 pKa = 4.39 NNVCTICEE38 pKa = 4.22 SGFEE42 pKa = 4.0 QYY44 pKa = 10.43 EE45 pKa = 4.23 PNVWTFRR52 pKa = 11.84 VVEE55 pKa = 4.21 KK56 pKa = 11.11 NKK58 pKa = 10.35 LNSIIYY64 pKa = 9.98 CNICAEE70 pKa = 4.7 EE71 pKa = 4.92 IDD73 pKa = 5.99 DD74 pKa = 5.7 DD75 pKa = 6.54 DD76 pKa = 4.7 EE77 pKa = 6.33 DD78 pKa = 4.3 CFKK81 pKa = 10.79 IALPASIEE89 pKa = 3.99 DD90 pKa = 3.34 QNRR93 pKa = 11.84 FEE95 pKa = 4.43 EE96 pKa = 4.37 LLDD99 pKa = 3.8 RR100 pKa = 11.84 LIEE103 pKa = 4.5 CGGVWLILNLVEE115 pKa = 5.7 YY116 pKa = 10.31 DD117 pKa = 3.22 NCVNGNSIDD126 pKa = 3.81 MEE128 pKa = 4.32 TEE130 pKa = 3.55 AFLKK134 pKa = 10.4 VLRR137 pKa = 11.84 FTSRR141 pKa = 11.84 DD142 pKa = 3.03 LDD144 pKa = 3.55 RR145 pKa = 11.84 PPNDD149 pKa = 3.3 KK150 pKa = 10.42 PGPINIIDD158 pKa = 4.12 KK159 pKa = 10.49 FCVSHH164 pKa = 6.57 SLRR167 pKa = 11.84 EE168 pKa = 3.96 NYY170 pKa = 9.69 INANILVDD178 pKa = 3.76 KK179 pKa = 10.76 LLNCTVDD186 pKa = 3.65 MLPKK190 pKa = 10.38 KK191 pKa = 10.55 LNNCSRR197 pKa = 11.84 EE198 pKa = 4.2 KK199 pKa = 10.52 FSHH202 pKa = 6.31 ALSTFKK208 pKa = 10.82 PNLLEE213 pKa = 5.62 DD214 pKa = 3.76 VLFSNTLVIPPEE226 pKa = 3.75 NLYY229 pKa = 9.38 TVMPTEE235 pKa = 4.93 LNTLKK240 pKa = 10.55 NQIYY244 pKa = 9.52 EE245 pKa = 4.12 VHH247 pKa = 5.99 QLNDD251 pKa = 4.05 DD252 pKa = 3.74 EE253 pKa = 7.02 DD254 pKa = 6.33 DD255 pKa = 4.68 NDD257 pKa = 4.0 DD258 pKa = 3.74 QEE260 pKa = 5.89 KK261 pKa = 10.87 DD262 pKa = 3.48 FSSRR266 pKa = 11.84 KK267 pKa = 8.21 VSEE270 pKa = 3.88 YY271 pKa = 10.98 LIDD274 pKa = 3.87 FLNDD278 pKa = 3.22 FVSNYY283 pKa = 10.43 NINPPNQHH291 pKa = 7.08 AIMPYY296 pKa = 9.68 CNSAAEE302 pKa = 3.93 AMYY305 pKa = 11.02 DD306 pKa = 3.34 FDD308 pKa = 5.67 LYY310 pKa = 11.53 VYY312 pKa = 10.78 APYY315 pKa = 11.04 DD316 pKa = 3.36 LL317 pKa = 5.52
Molecular weight: 36.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.896
IPC2_protein 4.151
IPC_protein 4.139
Toseland 3.948
ProMoST 4.266
Dawson 4.113
Bjellqvist 4.253
Wikipedia 3.999
Rodwell 3.973
Grimsley 3.859
Solomon 4.101
Lehninger 4.062
Nozaki 4.215
DTASelect 4.418
Thurlkill 3.973
EMBOSS 4.024
Sillero 4.253
Patrickios 1.952
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.133
Protein with the highest isoelectric point:
>tr|A0A0B4ZUP8|A0A0B4ZUP8_9ABAC Alkaline exonuclease OS=Pseudoplusia includens SNPV IE OX=1592335 GN=ORF-121 PE=4 SV=1
MM1 pKa = 7.29 VYY3 pKa = 10.2 RR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 SSGRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 SSGGQYY18 pKa = 9.83 RR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 SSGSRR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 SSGGRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 SSGGRR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 SSGGQYY48 pKa = 9.84 RR49 pKa = 11.84 RR50 pKa = 11.84 RR51 pKa = 11.84 SGSRR55 pKa = 11.84 RR56 pKa = 11.84 RR57 pKa = 11.84 SSGGRR62 pKa = 11.84 RR63 pKa = 11.84 RR64 pKa = 11.84 RR65 pKa = 11.84 PGRR68 pKa = 11.84 PRR70 pKa = 11.84 GRR72 pKa = 11.84 RR73 pKa = 11.84 SSSSGSRR80 pKa = 11.84 NPYY83 pKa = 7.93 GYY85 pKa = 10.07 SRR87 pKa = 11.84 RR88 pKa = 11.84 RR89 pKa = 11.84 YY90 pKa = 8.97
Molecular weight: 10.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.399
IPC2_protein 11.242
IPC_protein 12.471
Toseland 12.618
ProMoST 13.115
Dawson 12.618
Bjellqvist 12.618
Wikipedia 13.1
Rodwell 12.12
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.618
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 11.828
IPC_peptide 13.115
IPC2_peptide 12.106
IPC2.peptide.svr19 9.119
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
141
0
141
41865
49
1209
296.9
34.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.615 ± 0.157
2.069 ± 0.126
6.628 ± 0.173
5.587 ± 0.14
4.878 ± 0.146
3.363 ± 0.145
2.186 ± 0.103
7.835 ± 0.136
6.908 ± 0.25
8.969 ± 0.189
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.654 ± 0.081
7.949 ± 0.197
3.449 ± 0.148
3.669 ± 0.155
5.054 ± 0.166
7.123 ± 0.217
5.453 ± 0.128
6.012 ± 0.148
0.731 ± 0.053
4.844 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here