Amino acid dipepetide frequency for Vibrio phage Ares1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.165AlaAla: 7.165 ± 0.749
0.841AlaCys: 0.841 ± 0.193
3.723AlaAsp: 3.723 ± 0.348
5.804AlaGlu: 5.804 ± 0.617
3.002AlaPhe: 3.002 ± 0.373
4.643AlaGly: 4.643 ± 0.445
1.361AlaHis: 1.361 ± 0.236
5.684AlaIle: 5.684 ± 0.611
6.044AlaLys: 6.044 ± 0.541
8.006AlaLeu: 8.006 ± 0.651
2.522AlaMet: 2.522 ± 0.257
3.442AlaAsn: 3.442 ± 0.341
3.483AlaPro: 3.483 ± 0.316
3.723AlaGln: 3.723 ± 0.503
4.563AlaArg: 4.563 ± 0.421
4.723AlaSer: 4.723 ± 0.468
5.004AlaThr: 5.004 ± 0.499
5.884AlaVal: 5.884 ± 0.667
0.56AlaTrp: 0.56 ± 0.151
2.442AlaTyr: 2.442 ± 0.293
0.0AlaXaa: 0.0 ± 0.0
Cys
1.041CysAla: 1.041 ± 0.178
0.28CysCys: 0.28 ± 0.111
0.48CysAsp: 0.48 ± 0.147
0.841CysGlu: 0.841 ± 0.163
0.52CysPhe: 0.52 ± 0.14
1.081CysGly: 1.081 ± 0.207
0.32CysHis: 0.32 ± 0.106
0.68CysIle: 0.68 ± 0.197
0.761CysLys: 0.761 ± 0.182
1.441CysLeu: 1.441 ± 0.235
0.24CysMet: 0.24 ± 0.09
0.6CysAsn: 0.6 ± 0.134
0.48CysPro: 0.48 ± 0.149
0.4CysGln: 0.4 ± 0.14
0.721CysArg: 0.721 ± 0.157
0.64CysSer: 0.64 ± 0.16
1.121CysThr: 1.121 ± 0.246
0.721CysVal: 0.721 ± 0.171
0.12CysTrp: 0.12 ± 0.084
0.28CysTyr: 0.28 ± 0.094
0.0CysXaa: 0.0 ± 0.0
Asp
4.763AspAla: 4.763 ± 0.464
0.721AspCys: 0.721 ± 0.221
2.722AspAsp: 2.722 ± 0.345
4.003AspGlu: 4.003 ± 0.323
2.602AspPhe: 2.602 ± 0.312
3.082AspGly: 3.082 ± 0.346
1.681AspHis: 1.681 ± 0.264
3.322AspIle: 3.322 ± 0.386
4.203AspLys: 4.203 ± 0.563
7.005AspLeu: 7.005 ± 0.499
1.241AspMet: 1.241 ± 0.22
2.322AspAsn: 2.322 ± 0.292
3.963AspPro: 3.963 ± 0.408
2.682AspGln: 2.682 ± 0.38
3.442AspArg: 3.442 ± 0.371
2.842AspSer: 2.842 ± 0.326
4.563AspThr: 4.563 ± 0.404
3.763AspVal: 3.763 ± 0.362
0.56AspTrp: 0.56 ± 0.137
2.322AspTyr: 2.322 ± 0.255
0.0AspXaa: 0.0 ± 0.0
Glu
6.485GluAla: 6.485 ± 0.527
1.001GluCys: 1.001 ± 0.192
4.523GluAsp: 4.523 ± 0.424
3.883GluGlu: 3.883 ± 0.437
2.402GluPhe: 2.402 ± 0.325
4.563GluGly: 4.563 ± 0.492
1.481GluHis: 1.481 ± 0.21
4.603GluIle: 4.603 ± 0.369
3.643GluLys: 3.643 ± 0.431
7.245GluLeu: 7.245 ± 0.642
1.681GluMet: 1.681 ± 0.24
2.522GluAsn: 2.522 ± 0.297
2.001GluPro: 2.001 ± 0.278
3.523GluGln: 3.523 ± 0.48
3.442GluArg: 3.442 ± 0.354
3.483GluSer: 3.483 ± 0.481
4.323GluThr: 4.323 ± 0.399
4.763GluVal: 4.763 ± 0.369
0.52GluTrp: 0.52 ± 0.151
2.322GluTyr: 2.322 ± 0.316
0.0GluXaa: 0.0 ± 0.0
Phe
2.322PheAla: 2.322 ± 0.295
0.721PheCys: 0.721 ± 0.162
3.483PheAsp: 3.483 ± 0.492
2.122PheGlu: 2.122 ± 0.283
1.001PhePhe: 1.001 ± 0.176
2.081PheGly: 2.081 ± 0.289
0.721PheHis: 0.721 ± 0.177
2.842PheIle: 2.842 ± 0.328
2.882PheLys: 2.882 ± 0.334
2.842PheLeu: 2.842 ± 0.307
1.001PheMet: 1.001 ± 0.189
2.362PheAsn: 2.362 ± 0.341
1.001PhePro: 1.001 ± 0.203
0.961PheGln: 0.961 ± 0.193
1.321PheArg: 1.321 ± 0.212
2.402PheSer: 2.402 ± 0.268
2.081PheThr: 2.081 ± 0.236
2.322PheVal: 2.322 ± 0.271
0.04PheTrp: 0.04 ± 0.042
1.721PheTyr: 1.721 ± 0.253
0.0PheXaa: 0.0 ± 0.0
Gly
4.964GlyAla: 4.964 ± 0.462
0.841GlyCys: 0.841 ± 0.183
4.964GlyAsp: 4.964 ± 0.482
4.363GlyGlu: 4.363 ± 0.494
2.762GlyPhe: 2.762 ± 0.303
4.563GlyGly: 4.563 ± 0.562
1.121GlyHis: 1.121 ± 0.233
3.963GlyIle: 3.963 ± 0.375
3.242GlyLys: 3.242 ± 0.359
5.764GlyLeu: 5.764 ± 0.477
1.121GlyMet: 1.121 ± 0.261
3.362GlyAsn: 3.362 ± 0.39
0.0GlyPro: 0.0 ± 0.0
1.801GlyGln: 1.801 ± 0.248
2.842GlyArg: 2.842 ± 0.273
4.203GlySer: 4.203 ± 0.421
3.483GlyThr: 3.483 ± 0.405
4.964GlyVal: 4.964 ± 0.495
0.721GlyTrp: 0.721 ± 0.157
2.922GlyTyr: 2.922 ± 0.368
0.0GlyXaa: 0.0 ± 0.0
His
1.841HisAla: 1.841 ± 0.256
0.32HisCys: 0.32 ± 0.13
1.041HisAsp: 1.041 ± 0.207
1.481HisGlu: 1.481 ± 0.272
0.68HisPhe: 0.68 ± 0.162
1.481HisGly: 1.481 ± 0.243
0.44HisHis: 0.44 ± 0.137
0.841HisIle: 0.841 ± 0.167
1.521HisLys: 1.521 ± 0.223
2.122HisLeu: 2.122 ± 0.3
0.28HisMet: 0.28 ± 0.102
0.881HisAsn: 0.881 ± 0.206
0.921HisPro: 0.921 ± 0.188
0.36HisGln: 0.36 ± 0.107
1.241HisArg: 1.241 ± 0.221
1.161HisSer: 1.161 ± 0.244
1.361HisThr: 1.361 ± 0.194
1.601HisVal: 1.601 ± 0.267
0.12HisTrp: 0.12 ± 0.062
0.881HisTyr: 0.881 ± 0.185
0.0HisXaa: 0.0 ± 0.0
Ile
5.204IleAla: 5.204 ± 0.401
0.721IleCys: 0.721 ± 0.169
5.044IleAsp: 5.044 ± 0.464
5.284IleGlu: 5.284 ± 0.456
1.481IlePhe: 1.481 ± 0.272
3.162IleGly: 3.162 ± 0.389
0.921IleHis: 0.921 ± 0.205
3.162IleIle: 3.162 ± 0.416
5.444IleLys: 5.444 ± 0.437
4.443IleLeu: 4.443 ± 0.432
1.241IleMet: 1.241 ± 0.285
3.683IleAsn: 3.683 ± 0.38
3.643IlePro: 3.643 ± 0.391
2.362IleGln: 2.362 ± 0.257
3.563IleArg: 3.563 ± 0.361
3.803IleSer: 3.803 ± 0.435
4.083IleThr: 4.083 ± 0.44
3.843IleVal: 3.843 ± 0.368
0.36IleTrp: 0.36 ± 0.124
1.801IleTyr: 1.801 ± 0.329
0.0IleXaa: 0.0 ± 0.0
Lys
6.164LysAla: 6.164 ± 0.589
0.44LysCys: 0.44 ± 0.146
4.163LysAsp: 4.163 ± 0.498
4.803LysGlu: 4.803 ± 0.534
1.801LysPhe: 1.801 ± 0.264
3.483LysGly: 3.483 ± 0.344
1.681LysHis: 1.681 ± 0.302
3.362LysIle: 3.362 ± 0.385
5.124LysLys: 5.124 ± 0.592
6.365LysLeu: 6.365 ± 0.56
1.801LysMet: 1.801 ± 0.309
3.322LysAsn: 3.322 ± 0.331
4.523LysPro: 4.523 ± 0.445
3.082LysGln: 3.082 ± 0.361
3.683LysArg: 3.683 ± 0.419
3.883LysSer: 3.883 ± 0.448
4.243LysThr: 4.243 ± 0.436
3.002LysVal: 3.002 ± 0.284
0.6LysTrp: 0.6 ± 0.193
1.761LysTyr: 1.761 ± 0.269
0.0LysXaa: 0.0 ± 0.0
Leu
7.726LeuAla: 7.726 ± 0.659
1.441LeuCys: 1.441 ± 0.222
5.924LeuAsp: 5.924 ± 0.453
6.044LeuGlu: 6.044 ± 0.437
3.242LeuPhe: 3.242 ± 0.371
5.284LeuGly: 5.284 ± 0.438
2.202LeuHis: 2.202 ± 0.368
7.285LeuIle: 7.285 ± 0.613
5.404LeuLys: 5.404 ± 0.438
6.925LeuLeu: 6.925 ± 0.731
2.202LeuMet: 2.202 ± 0.302
5.764LeuAsn: 5.764 ± 0.453
3.643LeuPro: 3.643 ± 0.389
4.283LeuGln: 4.283 ± 0.442
4.123LeuArg: 4.123 ± 0.385
6.405LeuSer: 6.405 ± 0.434
5.364LeuThr: 5.364 ± 0.449
4.683LeuVal: 4.683 ± 0.422
0.56LeuTrp: 0.56 ± 0.168
2.882LeuTyr: 2.882 ± 0.324
0.0LeuXaa: 0.0 ± 0.0
Met
2.482MetAla: 2.482 ± 0.268
0.4MetCys: 0.4 ± 0.146
0.961MetAsp: 0.961 ± 0.173
0.841MetGlu: 0.841 ± 0.216
0.921MetPhe: 0.921 ± 0.19
1.321MetGly: 1.321 ± 0.212
0.6MetHis: 0.6 ± 0.156
1.321MetIle: 1.321 ± 0.216
1.641MetLys: 1.641 ± 0.259
1.841MetLeu: 1.841 ± 0.272
0.4MetMet: 0.4 ± 0.14
1.281MetAsn: 1.281 ± 0.222
0.68MetPro: 0.68 ± 0.188
1.161MetGln: 1.161 ± 0.245
1.361MetArg: 1.361 ± 0.237
1.281MetSer: 1.281 ± 0.259
1.441MetThr: 1.441 ± 0.256
1.321MetVal: 1.321 ± 0.207
0.32MetTrp: 0.32 ± 0.113
0.68MetTyr: 0.68 ± 0.163
0.0MetXaa: 0.0 ± 0.0
Asn
4.843AsnAla: 4.843 ± 0.517
0.721AsnCys: 0.721 ± 0.155
3.042AsnAsp: 3.042 ± 0.342
3.122AsnGlu: 3.122 ± 0.394
2.402AsnPhe: 2.402 ± 0.39
3.723AsnGly: 3.723 ± 0.462
0.841AsnHis: 0.841 ± 0.163
3.162AsnIle: 3.162 ± 0.373
3.523AsnLys: 3.523 ± 0.417
4.403AsnLeu: 4.403 ± 0.497
1.081AsnMet: 1.081 ± 0.208
2.362AsnAsn: 2.362 ± 0.291
2.882AsnPro: 2.882 ± 0.398
1.561AsnGln: 1.561 ± 0.254
2.882AsnArg: 2.882 ± 0.335
2.602AsnSer: 2.602 ± 0.308
3.282AsnThr: 3.282 ± 0.396
2.882AsnVal: 2.882 ± 0.45
0.36AsnTrp: 0.36 ± 0.11
1.801AsnTyr: 1.801 ± 0.27
0.0AsnXaa: 0.0 ± 0.0
Pro
1.761ProAla: 1.761 ± 0.234
0.56ProCys: 0.56 ± 0.15
2.882ProAsp: 2.882 ± 0.326
3.242ProGlu: 3.242 ± 0.27
1.321ProPhe: 1.321 ± 0.247
2.282ProGly: 2.282 ± 0.308
1.041ProHis: 1.041 ± 0.249
2.081ProIle: 2.081 ± 0.263
3.402ProLys: 3.402 ± 0.381
3.242ProLeu: 3.242 ± 0.352
0.6ProMet: 0.6 ± 0.143
2.041ProAsn: 2.041 ± 0.295
1.561ProPro: 1.561 ± 0.223
1.401ProGln: 1.401 ± 0.221
2.162ProArg: 2.162 ± 0.342
2.362ProSer: 2.362 ± 0.304
3.282ProThr: 3.282 ± 0.436
3.282ProVal: 3.282 ± 0.407
0.52ProTrp: 0.52 ± 0.137
1.121ProTyr: 1.121 ± 0.26
0.0ProXaa: 0.0 ± 0.0
Gln
3.803GlnAla: 3.803 ± 0.46
0.44GlnCys: 0.44 ± 0.129
2.001GlnAsp: 2.001 ± 0.293
2.322GlnGlu: 2.322 ± 0.301
1.401GlnPhe: 1.401 ± 0.196
2.842GlnGly: 2.842 ± 0.372
0.48GlnHis: 0.48 ± 0.135
2.882GlnIle: 2.882 ± 0.318
2.081GlnLys: 2.081 ± 0.349
4.723GlnLeu: 4.723 ± 0.483
0.761GlnMet: 0.761 ± 0.166
1.521GlnAsn: 1.521 ± 0.24
1.201GlnPro: 1.201 ± 0.233
1.721GlnGln: 1.721 ± 0.255
2.081GlnArg: 2.081 ± 0.446
2.242GlnSer: 2.242 ± 0.365
2.242GlnThr: 2.242 ± 0.316
2.482GlnVal: 2.482 ± 0.371
0.48GlnTrp: 0.48 ± 0.12
1.161GlnTyr: 1.161 ± 0.26
0.0GlnXaa: 0.0 ± 0.0
Arg
4.443ArgAla: 4.443 ± 0.47
0.36ArgCys: 0.36 ± 0.111
2.562ArgAsp: 2.562 ± 0.288
4.603ArgGlu: 4.603 ± 0.587
2.402ArgPhe: 2.402 ± 0.337
3.082ArgGly: 3.082 ± 0.324
0.921ArgHis: 0.921 ± 0.206
3.162ArgIle: 3.162 ± 0.35
3.362ArgLys: 3.362 ± 0.495
4.363ArgLeu: 4.363 ± 0.373
0.761ArgMet: 0.761 ± 0.169
2.722ArgAsn: 2.722 ± 0.312
1.641ArgPro: 1.641 ± 0.24
1.961ArgGln: 1.961 ± 0.373
3.322ArgArg: 3.322 ± 0.442
2.922ArgSer: 2.922 ± 0.381
2.962ArgThr: 2.962 ± 0.292
4.283ArgVal: 4.283 ± 0.378
0.36ArgTrp: 0.36 ± 0.11
1.841ArgTyr: 1.841 ± 0.24
0.0ArgXaa: 0.0 ± 0.0
Ser
4.483SerAla: 4.483 ± 0.577
0.6SerCys: 0.6 ± 0.175
3.563SerAsp: 3.563 ± 0.434
3.803SerGlu: 3.803 ± 0.507
2.122SerPhe: 2.122 ± 0.379
4.603SerGly: 4.603 ± 0.51
1.321SerHis: 1.321 ± 0.202
4.323SerIle: 4.323 ± 0.411
4.083SerLys: 4.083 ± 0.393
5.724SerLeu: 5.724 ± 0.443
1.361SerMet: 1.361 ± 0.181
3.523SerAsn: 3.523 ± 0.374
2.202SerPro: 2.202 ± 0.329
2.001SerGln: 2.001 ± 0.253
2.522SerArg: 2.522 ± 0.288
3.442SerSer: 3.442 ± 0.507
3.643SerThr: 3.643 ± 0.406
3.843SerVal: 3.843 ± 0.337
0.24SerTrp: 0.24 ± 0.091
2.081SerTyr: 2.081 ± 0.306
0.0SerXaa: 0.0 ± 0.0
Thr
4.924ThrAla: 4.924 ± 0.389
0.64ThrCys: 0.64 ± 0.132
3.843ThrAsp: 3.843 ± 0.365
4.163ThrGlu: 4.163 ± 0.412
2.602ThrPhe: 2.602 ± 0.322
5.124ThrGly: 5.124 ± 0.444
1.201ThrHis: 1.201 ± 0.203
4.283ThrIle: 4.283 ± 0.428
3.723ThrLys: 3.723 ± 0.467
6.244ThrLeu: 6.244 ± 0.587
1.241ThrMet: 1.241 ± 0.187
3.122ThrAsn: 3.122 ± 0.375
2.602ThrPro: 2.602 ± 0.315
2.602ThrGln: 2.602 ± 0.319
3.042ThrArg: 3.042 ± 0.297
2.962ThrSer: 2.962 ± 0.29
4.803ThrThr: 4.803 ± 0.585
4.683ThrVal: 4.683 ± 0.447
0.56ThrTrp: 0.56 ± 0.127
1.721ThrTyr: 1.721 ± 0.286
0.0ThrXaa: 0.0 ± 0.0
Val
4.683ValAla: 4.683 ± 0.426
1.081ValCys: 1.081 ± 0.212
3.883ValAsp: 3.883 ± 0.366
5.284ValGlu: 5.284 ± 0.547
2.442ValPhe: 2.442 ± 0.279
3.202ValGly: 3.202 ± 0.354
1.121ValHis: 1.121 ± 0.185
3.963ValIle: 3.963 ± 0.32
4.884ValLys: 4.884 ± 0.429
4.603ValLeu: 4.603 ± 0.447
1.641ValMet: 1.641 ± 0.274
3.923ValAsn: 3.923 ± 0.393
2.242ValPro: 2.242 ± 0.317
1.401ValGln: 1.401 ± 0.222
3.883ValArg: 3.883 ± 0.327
5.244ValSer: 5.244 ± 0.383
4.363ValThr: 4.363 ± 0.514
4.243ValVal: 4.243 ± 0.464
0.36ValTrp: 0.36 ± 0.116
2.001ValTyr: 2.001 ± 0.3
0.0ValXaa: 0.0 ± 0.0
Trp
0.56TrpAla: 0.56 ± 0.145
0.08TrpCys: 0.08 ± 0.058
0.44TrpAsp: 0.44 ± 0.138
0.48TrpGlu: 0.48 ± 0.142
0.08TrpPhe: 0.08 ± 0.054
0.52TrpGly: 0.52 ± 0.17
0.2TrpHis: 0.2 ± 0.108
0.761TrpIle: 0.761 ± 0.167
0.36TrpLys: 0.36 ± 0.134
1.121TrpLeu: 1.121 ± 0.219
0.28TrpMet: 0.28 ± 0.088
0.36TrpAsn: 0.36 ± 0.122
0.0TrpPro: 0.0 ± 0.0
0.44TrpGln: 0.44 ± 0.114
0.24TrpArg: 0.24 ± 0.087
0.52TrpSer: 0.52 ± 0.143
0.32TrpThr: 0.32 ± 0.108
0.48TrpVal: 0.48 ± 0.146
0.2TrpTrp: 0.2 ± 0.083
0.32TrpTyr: 0.32 ± 0.115
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.962TyrAla: 2.962 ± 0.392
0.56TyrCys: 0.56 ± 0.168
2.562TyrAsp: 2.562 ± 0.312
1.921TyrGlu: 1.921 ± 0.25
1.041TyrPhe: 1.041 ± 0.168
1.841TyrGly: 1.841 ± 0.31
0.921TyrHis: 0.921 ± 0.192
1.561TyrIle: 1.561 ± 0.198
2.001TyrLys: 2.001 ± 0.33
2.962TyrLeu: 2.962 ± 0.336
0.761TyrMet: 0.761 ± 0.164
2.362TyrAsn: 2.362 ± 0.27
1.481TyrPro: 1.481 ± 0.263
1.441TyrGln: 1.441 ± 0.242
1.601TyrArg: 1.601 ± 0.25
2.362TyrSer: 2.362 ± 0.361
2.122TyrThr: 2.122 ± 0.307
1.401TyrVal: 1.401 ± 0.236
0.2TyrTrp: 0.2 ± 0.087
1.081TyrTyr: 1.081 ± 0.217
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 119 proteins (24983 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski