alpha proteobacterium IMCC14465

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Alphaproteobacteria incertae sedis; PS1 clade

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1890 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9E2Z6|J9E2Z6_9PROT ATP-dependent Clp protease proteolytic subunit OS=alpha proteobacterium IMCC14465 OX=1220535 GN=clpP PE=3 SV=1
MM1 pKa = 7.73EE2 pKa = 4.81NTHH5 pKa = 7.01DD6 pKa = 3.6TDD8 pKa = 3.58DD9 pKa = 3.76WGEE12 pKa = 3.92EE13 pKa = 4.08MDD15 pKa = 4.71SSEE18 pKa = 5.6HH19 pKa = 6.01GNTYY23 pKa = 11.09NLFLGIIKK31 pKa = 9.51WGSIAVTAILGFLLFFVYY49 pKa = 9.82FF50 pKa = 4.03

Molecular weight: 5.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9DV44|J9DV44_9PROT Uncharacterized protein OS=alpha proteobacterium IMCC14465 OX=1220535 GN=IMCC14465_06980 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.47NGRR28 pKa = 11.84LVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1890

0

1890

589740

37

1590

312.0

34.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.671 ± 0.053

0.937 ± 0.018

6.301 ± 0.048

6.356 ± 0.057

4.097 ± 0.039

7.629 ± 0.048

2.08 ± 0.03

6.395 ± 0.045

4.822 ± 0.043

9.821 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.831 ± 0.026

3.952 ± 0.036

4.425 ± 0.039

3.563 ± 0.028

5.21 ± 0.043

6.162 ± 0.044

5.484 ± 0.046

6.586 ± 0.043

1.084 ± 0.022

2.594 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski