Amborella trichopoda

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Amborellales; Amborellaceae; Amborella

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27371 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5D6G4|U5D6G4_AMBTC Uncharacterized protein OS=Amborella trichopoda OX=13333 GN=AMTR_s00047p00191390 PE=4 SV=1
MM1 pKa = 7.68PCVFLAWTYY10 pKa = 11.39GRR12 pKa = 11.84GSNLSLGNGCKK23 pKa = 10.57DD24 pKa = 3.65MFWDD28 pKa = 5.42DD29 pKa = 3.04IMFVVNVLGAGNGCKK44 pKa = 10.25DD45 pKa = 4.39LFWDD49 pKa = 5.17DD50 pKa = 2.92IMFVVNVLGAGNGCRR65 pKa = 11.84DD66 pKa = 4.11MFWDD70 pKa = 5.28DD71 pKa = 3.22IMFVVNVLGAGNGCKK86 pKa = 10.52DD87 pKa = 3.9MFWDD91 pKa = 6.1DD92 pKa = 3.43ILFVANVLGAGNGCKK107 pKa = 10.52DD108 pKa = 3.92MFWSDD113 pKa = 2.92ILFVANN119 pKa = 4.21

Molecular weight: 13.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W1NI78|W1NI78_AMBTC Serine/threonine-protein phosphatase OS=Amborella trichopoda OX=13333 GN=AMTR_s00009p00134520 PE=3 SV=1
MM1 pKa = 7.26KK2 pKa = 10.39KK3 pKa = 9.73KK4 pKa = 10.31RR5 pKa = 11.84KK6 pKa = 8.54RR7 pKa = 11.84KK8 pKa = 9.06KK9 pKa = 9.19RR10 pKa = 11.84KK11 pKa = 7.77EE12 pKa = 3.81RR13 pKa = 11.84KK14 pKa = 8.91KK15 pKa = 10.6KK16 pKa = 9.26KK17 pKa = 9.8KK18 pKa = 9.6KK19 pKa = 9.42RR20 pKa = 11.84KK21 pKa = 8.09KK22 pKa = 9.86KK23 pKa = 9.09KK24 pKa = 8.95RR25 pKa = 11.84KK26 pKa = 9.09KK27 pKa = 8.97KK28 pKa = 9.96RR29 pKa = 11.84EE30 pKa = 3.96RR31 pKa = 11.84KK32 pKa = 9.49RR33 pKa = 11.84KK34 pKa = 6.8TSKK37 pKa = 10.14KK38 pKa = 9.32RR39 pKa = 11.84RR40 pKa = 11.84KK41 pKa = 9.31KK42 pKa = 9.63KK43 pKa = 10.13KK44 pKa = 9.34KK45 pKa = 9.47RR46 pKa = 11.84KK47 pKa = 9.27KK48 pKa = 9.68KK49 pKa = 10.17KK50 pKa = 9.92KK51 pKa = 9.0NNKK54 pKa = 7.58KK55 pKa = 10.09RR56 pKa = 11.84KK57 pKa = 8.09RR58 pKa = 11.84KK59 pKa = 8.61KK60 pKa = 8.52KK61 pKa = 9.56KK62 pKa = 8.34RR63 pKa = 11.84RR64 pKa = 11.84KK65 pKa = 8.87RR66 pKa = 11.84RR67 pKa = 11.84KK68 pKa = 8.01KK69 pKa = 9.32RR70 pKa = 11.84KK71 pKa = 8.59RR72 pKa = 11.84RR73 pKa = 11.84KK74 pKa = 8.33KK75 pKa = 9.22KK76 pKa = 9.51KK77 pKa = 8.54RR78 pKa = 11.84RR79 pKa = 11.84KK80 pKa = 8.05KK81 pKa = 9.37RR82 pKa = 11.84RR83 pKa = 11.84RR84 pKa = 11.84KK85 pKa = 8.61RR86 pKa = 11.84RR87 pKa = 11.84RR88 pKa = 11.84RR89 pKa = 11.84RR90 pKa = 11.84KK91 pKa = 9.02RR92 pKa = 11.84GKK94 pKa = 9.64KK95 pKa = 8.93RR96 pKa = 11.84RR97 pKa = 11.84KK98 pKa = 9.38KK99 pKa = 9.97KK100 pKa = 9.65RR101 pKa = 11.84KK102 pKa = 8.08KK103 pKa = 10.04KK104 pKa = 9.85KK105 pKa = 9.26RR106 pKa = 11.84KK107 pKa = 9.05KK108 pKa = 8.92KK109 pKa = 9.67KK110 pKa = 9.21KK111 pKa = 8.79RR112 pKa = 11.84KK113 pKa = 9.51KK114 pKa = 8.34RR115 pKa = 11.84TKK117 pKa = 9.79KK118 pKa = 9.27RR119 pKa = 11.84KK120 pKa = 8.87KK121 pKa = 9.55RR122 pKa = 11.84KK123 pKa = 9.43KK124 pKa = 9.75KK125 pKa = 10.28

Molecular weight: 16.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27366

5

27371

8596660

29

4990

314.1

35.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.804 ± 0.013

1.908 ± 0.008

5.026 ± 0.011

6.681 ± 0.019

4.108 ± 0.011

6.925 ± 0.018

2.501 ± 0.008

5.154 ± 0.011

5.78 ± 0.016

9.916 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.592 ± 0.007

4.118 ± 0.011

5.199 ± 0.017

3.558 ± 0.012

5.662 ± 0.013

8.852 ± 0.018

4.756 ± 0.009

6.466 ± 0.012

1.339 ± 0.006

2.654 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski