Vesicular stomatitis New Jersey virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Vesiculovirus; New Jersey vesiculovirus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7D459|I7D459_9RHAB Nucleocapsid protein OS=Vesicular stomatitis New Jersey virus OX=11280 PE=3 SV=1
MM1 pKa = 8.11DD2 pKa = 3.75SVEE5 pKa = 4.05RR6 pKa = 11.84LKK8 pKa = 10.32TYY10 pKa = 10.47LATYY14 pKa = 10.48DD15 pKa = 4.05NLDD18 pKa = 4.09SALQDD23 pKa = 3.63ANEE26 pKa = 4.16SEE28 pKa = 4.15ARR30 pKa = 11.84RR31 pKa = 11.84EE32 pKa = 3.91DD33 pKa = 4.82KK34 pKa = 10.98YY35 pKa = 11.52LQDD38 pKa = 5.98LFIEE42 pKa = 4.58DD43 pKa = 4.04QGDD46 pKa = 3.64KK47 pKa = 8.78PTPSYY52 pKa = 10.19YY53 pKa = 10.06QEE55 pKa = 4.54EE56 pKa = 4.47EE57 pKa = 4.43SSDD60 pKa = 3.45SDD62 pKa = 3.11TDD64 pKa = 3.66YY65 pKa = 11.37NAEE68 pKa = 4.28HH69 pKa = 6.68LTMLSPDD76 pKa = 3.2EE77 pKa = 6.02RR78 pKa = 11.84IDD80 pKa = 3.24KK81 pKa = 10.3WEE83 pKa = 3.96EE84 pKa = 3.59DD85 pKa = 3.67LPEE88 pKa = 4.77LEE90 pKa = 5.82KK91 pKa = 10.31IDD93 pKa = 5.26DD94 pKa = 5.31DD95 pKa = 4.61IPVTFSDD102 pKa = 3.17WTQPVMKK109 pKa = 9.88EE110 pKa = 3.77NGGEE114 pKa = 3.88KK115 pKa = 10.03SLSLFHH121 pKa = 6.83PVGLTKK127 pKa = 10.21IQTDD131 pKa = 3.1QWRR134 pKa = 11.84KK135 pKa = 7.6TIEE138 pKa = 4.09AVCEE142 pKa = 4.01SSKK145 pKa = 10.81YY146 pKa = 9.3WNLSEE151 pKa = 4.03CQIMNSDD158 pKa = 3.1NCLILKK164 pKa = 9.35GRR166 pKa = 11.84VMTTDD171 pKa = 3.56CSSSIKK177 pKa = 10.07SQNSIQSSEE186 pKa = 4.37SFSSSHH192 pKa = 6.36SPSPAPKK199 pKa = 8.46VTEE202 pKa = 4.27STSLWDD208 pKa = 3.65LRR210 pKa = 11.84STEE213 pKa = 3.84VQLISKK219 pKa = 9.9RR220 pKa = 11.84AGVKK224 pKa = 10.79DD225 pKa = 3.63MMVKK229 pKa = 9.47LTDD232 pKa = 3.82FFEE235 pKa = 6.39SEE237 pKa = 3.93DD238 pKa = 3.97EE239 pKa = 4.49YY240 pKa = 11.77YY241 pKa = 10.8SVCPEE246 pKa = 4.24GAPDD250 pKa = 3.98LMGAIIMGLKK260 pKa = 9.59HH261 pKa = 6.63KK262 pKa = 10.68KK263 pKa = 9.95LFNQARR269 pKa = 11.84VKK271 pKa = 10.82YY272 pKa = 9.91RR273 pKa = 11.84LL274 pKa = 3.35

Molecular weight: 31.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9DGV5|Q9DGV5_9RHAB Glycoprotein OS=Vesicular stomatitis New Jersey virus OX=11280 PE=3 SV=1
MM1 pKa = 7.72SSFKK5 pKa = 10.64KK6 pKa = 10.44ILGLSSKK13 pKa = 7.53SHH15 pKa = 6.15KK16 pKa = 9.86KK17 pKa = 9.73SKK19 pKa = 10.64KK20 pKa = 9.85LGLPPPYY27 pKa = 10.09DD28 pKa = 3.46EE29 pKa = 4.92SSPMEE34 pKa = 4.19IQPSAPLSNDD44 pKa = 2.95FFGMEE49 pKa = 5.58DD50 pKa = 3.18MDD52 pKa = 6.38LYY54 pKa = 11.49DD55 pKa = 5.09KK56 pKa = 11.34DD57 pKa = 3.49SLRR60 pKa = 11.84YY61 pKa = 8.84EE62 pKa = 4.36KK63 pKa = 10.58FRR65 pKa = 11.84FMLKK69 pKa = 7.95MTVRR73 pKa = 11.84SNKK76 pKa = 9.31PFRR79 pKa = 11.84SYY81 pKa = 11.86DD82 pKa = 3.46DD83 pKa = 3.16VTAAVSQWDD92 pKa = 3.41NSYY95 pKa = 10.93IGMVGKK101 pKa = 10.57RR102 pKa = 11.84PFYY105 pKa = 10.59KK106 pKa = 9.95IIALIGSSHH115 pKa = 6.42LQATPAVLADD125 pKa = 4.27LNQPEE130 pKa = 4.8YY131 pKa = 10.9YY132 pKa = 9.36ATLTGRR138 pKa = 11.84CFLPHH143 pKa = 7.26RR144 pKa = 11.84LGLIPPMFNVSEE156 pKa = 4.38TFRR159 pKa = 11.84KK160 pKa = 9.19PFNIGIYY167 pKa = 10.12KK168 pKa = 8.76GTLDD172 pKa = 3.54FTFTVSDD179 pKa = 3.91DD180 pKa = 3.65EE181 pKa = 5.1SNEE184 pKa = 4.04KK185 pKa = 10.0VPHH188 pKa = 4.81VWEE191 pKa = 4.14YY192 pKa = 10.96MNPKK196 pKa = 9.37YY197 pKa = 10.39QSQIQKK203 pKa = 10.19EE204 pKa = 4.28GLKK207 pKa = 10.63FGLILSKK214 pKa = 10.55KK215 pKa = 9.05ATGTWVLDD223 pKa = 3.31QLSPFKK229 pKa = 11.04

Molecular weight: 26.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3551

229

2109

710.2

81.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.252 ± 0.636

1.774 ± 0.284

6.308 ± 0.707

5.914 ± 0.508

4.252 ± 0.361

6.055 ± 0.522

2.534 ± 0.392

7.294 ± 0.744

7.125 ± 0.452

9.406 ± 0.659

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.844 ± 0.259

4.083 ± 0.483

5.21 ± 0.464

3.295 ± 0.251

4.9 ± 0.494

8.42 ± 0.641

5.604 ± 0.42

4.928 ± 0.462

1.971 ± 0.133

3.83 ± 0.293
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski