Fusarium graminearum deltaflexivirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Deltaflexiviridae; Deltaflexivirus; Fusarium deltaflexivirus 1

Average proteome isoelectric point is 7.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1JH97|A0A1B1JH97_9VIRU Uncharacterized protein OS=Fusarium graminearum deltaflexivirus 1 OX=1872710 PE=4 SV=1
MM1 pKa = 7.71SEE3 pKa = 4.62FIGDD7 pKa = 3.65TSHH10 pKa = 7.55LKK12 pKa = 10.27RR13 pKa = 11.84DD14 pKa = 3.87TVPFSAVFTFEE25 pKa = 4.2GVSGSGAVKK34 pKa = 9.3LTDD37 pKa = 3.39NLSISRR43 pKa = 11.84IIAGRR48 pKa = 11.84AYY50 pKa = 10.84VSLDD54 pKa = 3.33PSSLTIEE61 pKa = 5.14LISPPSPDD69 pKa = 3.13KK70 pKa = 10.9AAHH73 pKa = 5.63LTAAVIPSTVSSPPKK88 pKa = 9.17LTEE91 pKa = 5.05HH92 pKa = 7.05ILTVPGSVLIRR103 pKa = 11.84SSTYY107 pKa = 9.95DD108 pKa = 3.05TTLPSSIQLAPGVAHH123 pKa = 6.08QLVPPPLIGDD133 pKa = 4.13PPQLVFAYY141 pKa = 9.38HH142 pKa = 6.58IAGASSDD149 pKa = 3.89TKK151 pKa = 10.63GHH153 pKa = 5.37VRR155 pKa = 11.84LSGTITLGGVGFIQTWW171 pKa = 2.94

Molecular weight: 17.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1JH96|A0A1B1JH96_9VIRU Uncharacterized protein OS=Fusarium graminearum deltaflexivirus 1 OX=1872710 PE=4 SV=1
MM1 pKa = 7.76LDD3 pKa = 3.45SPLVLTLVINFFISCFLWASRR24 pKa = 11.84PEE26 pKa = 4.22WNFGLAARR34 pKa = 11.84FFFAFPFWSVLLIFFTCWLIGSIRR58 pKa = 11.84DD59 pKa = 3.27CWLFRR64 pKa = 11.84LVRR67 pKa = 11.84TSYY70 pKa = 8.96THH72 pKa = 7.05SKK74 pKa = 8.72VQQLPTYY81 pKa = 7.37TAPAPARR88 pKa = 11.84VRR90 pKa = 11.84ADD92 pKa = 4.77PIPSAVSGDD101 pKa = 3.48IASAAPVTPRR111 pKa = 11.84SATRR115 pKa = 11.84FQFF118 pKa = 3.58

Molecular weight: 13.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2590

104

2042

518.0

57.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.228 ± 0.423

1.236 ± 0.356

5.212 ± 0.689

2.432 ± 0.485

5.174 ± 1.262

5.792 ± 0.787

3.552 ± 0.359

3.243 ± 0.937

2.78 ± 0.437

10.618 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.467 ± 0.244

2.587 ± 0.463

9.228 ± 0.634

3.127 ± 0.364

6.1 ± 0.783

7.529 ± 1.272

7.645 ± 0.677

7.452 ± 0.187

2.471 ± 0.692

3.127 ± 0.457
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski