Sweet potato chlorotic fleck virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 7.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5L4G3|Q5L4G3_9VIRU 7 kDa protein OS=Sweet potato chlorotic fleck virus OX=263004 PE=3 SV=1
MM1 pKa = 7.35AAKK4 pKa = 10.0EE5 pKa = 4.1ADD7 pKa = 3.31TMEE10 pKa = 4.51KK11 pKa = 10.46VEE13 pKa = 4.14SSKK16 pKa = 11.15GKK18 pKa = 10.1EE19 pKa = 3.8KK20 pKa = 11.01AGGQTTTLEE29 pKa = 4.19GLNEE33 pKa = 3.89VKK35 pKa = 10.43AAQGEE40 pKa = 4.34ISDD43 pKa = 3.96QQLMKK48 pKa = 10.44HH49 pKa = 6.31DD50 pKa = 5.44ALIKK54 pKa = 9.69WYY56 pKa = 9.06MEE58 pKa = 3.83NYY60 pKa = 9.7RR61 pKa = 11.84PSDD64 pKa = 3.45VVNPLMQSGDD74 pKa = 3.37KK75 pKa = 10.77HH76 pKa = 5.13VTLSDD81 pKa = 3.53NLKK84 pKa = 10.07EE85 pKa = 4.1DD86 pKa = 3.15AANIYY91 pKa = 10.22SRR93 pKa = 11.84PNFNTLLKK101 pKa = 9.8WNRR104 pKa = 11.84EE105 pKa = 3.97PVSQSIATAEE115 pKa = 4.72DD116 pKa = 3.24IAQIEE121 pKa = 4.18AMLVGLGIPQEE132 pKa = 4.18RR133 pKa = 11.84VRR135 pKa = 11.84TAILDD140 pKa = 3.52IVLYY144 pKa = 9.83AAHH147 pKa = 6.83SSSSPQQLYY156 pKa = 10.59EE157 pKa = 3.93GDD159 pKa = 3.24IDD161 pKa = 5.02FRR163 pKa = 11.84DD164 pKa = 3.78ANFKK168 pKa = 10.13EE169 pKa = 4.69AISRR173 pKa = 11.84SSVAAVIKK181 pKa = 10.56DD182 pKa = 3.05KK183 pKa = 10.84STIRR187 pKa = 11.84KK188 pKa = 7.54VCRR191 pKa = 11.84LFAPVVWSYY200 pKa = 10.57MIINNEE206 pKa = 4.23PPSGWQAKK214 pKa = 8.58GFPEE218 pKa = 4.02NAKK221 pKa = 10.07FAAFDD226 pKa = 3.46TFDD229 pKa = 3.4FVTNHH234 pKa = 6.19AAIKK238 pKa = 8.77PLEE241 pKa = 4.88GIARR245 pKa = 11.84PPNSVEE251 pKa = 3.98YY252 pKa = 10.33IAAQTSKK259 pKa = 11.01RR260 pKa = 11.84ILIDD264 pKa = 3.11KK265 pKa = 10.12ARR267 pKa = 11.84RR268 pKa = 11.84NEE270 pKa = 3.8KK271 pKa = 10.17LSNYY275 pKa = 6.89EE276 pKa = 3.73ASVTGGQQACEE287 pKa = 3.93IKK289 pKa = 10.03TEE291 pKa = 4.13LKK293 pKa = 11.21GNGKK297 pKa = 9.85CKK299 pKa = 10.55

Molecular weight: 33.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5L4G2|Q5L4G2_9VIRU Capsid protein OS=Sweet potato chlorotic fleck virus OX=263004 PE=3 SV=1
MM1 pKa = 7.42GSASEE6 pKa = 4.07RR7 pKa = 11.84LPNFVATFLYY17 pKa = 10.64GRR19 pKa = 11.84GLLCDD24 pKa = 4.04WDD26 pKa = 4.18CCLLISSIVSNYY38 pKa = 8.6NKK40 pKa = 10.46GMRR43 pKa = 11.84TYY45 pKa = 11.23KK46 pKa = 10.44FFACGQSKK54 pKa = 10.38SAIKK58 pKa = 10.17RR59 pKa = 11.84RR60 pKa = 11.84ARR62 pKa = 11.84RR63 pKa = 11.84MQICPRR69 pKa = 11.84CAKK72 pKa = 10.04YY73 pKa = 10.58EE74 pKa = 4.09CGKK77 pKa = 10.21KK78 pKa = 10.23CEE80 pKa = 4.38PNTYY84 pKa = 10.21SQLQVKK90 pKa = 9.3EE91 pKa = 4.06LVTVGVNRR99 pKa = 11.84YY100 pKa = 4.18TTEE103 pKa = 4.27NITKK107 pKa = 10.17KK108 pKa = 8.31GTNVRR113 pKa = 11.84RR114 pKa = 11.84HH115 pKa = 6.1CKK117 pKa = 10.33NEE119 pKa = 3.72LEE121 pKa = 4.4FIKK124 pKa = 11.1YY125 pKa = 9.49EE126 pKa = 4.07SLKK129 pKa = 10.76VKK131 pKa = 10.5PKK133 pKa = 10.6

Molecular weight: 15.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2937

67

2090

489.5

55.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.958 ± 0.913

2.656 ± 0.807

5.175 ± 0.565

6.878 ± 1.056

5.856 ± 0.933

6.435 ± 0.362

1.736 ± 0.233

5.993 ± 0.675

7.116 ± 0.701

9.057 ± 0.575

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.805 ± 0.254

4.699 ± 0.569

3.541 ± 0.588

2.826 ± 0.353

5.958 ± 0.589

7.729 ± 0.479

5.312 ± 0.525

6.639 ± 0.817

0.851 ± 0.166

3.779 ± 0.35
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski