Garlic virus E
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8QXW5|Q8QXW5_9VIRU RNA-directed RNA polymerase OS=Garlic virus E OX=150285 PE=4 SV=1
MM1 pKa = 6.78 TTVQKK6 pKa = 11.0 LLDD9 pKa = 4.13 QISDD13 pKa = 3.75 PSARR17 pKa = 11.84 AAYY20 pKa = 10.18 SQDD23 pKa = 3.42 CLSAAKK29 pKa = 8.78 TAATKK34 pKa = 11.09 AMTIAPYY41 pKa = 10.78 AVTPTEE47 pKa = 3.96 ALTLEE52 pKa = 4.78 RR53 pKa = 11.84 IGITTSPFATTSHH66 pKa = 4.89 THH68 pKa = 5.93 AADD71 pKa = 4.1 KK72 pKa = 10.63 IIEE75 pKa = 4.28 NDD77 pKa = 3.51 CLDD80 pKa = 3.67 IIGRR84 pKa = 11.84 YY85 pKa = 8.46 LPKK88 pKa = 10.68 EE89 pKa = 4.03 PVTLIQLKK97 pKa = 9.75 RR98 pKa = 11.84 AKK100 pKa = 10.22 LHH102 pKa = 5.63 LLRR105 pKa = 11.84 RR106 pKa = 11.84 GPSGDD111 pKa = 2.8 IFKK114 pKa = 10.65 NYY116 pKa = 9.13 CHH118 pKa = 6.56 EE119 pKa = 4.62 PKK121 pKa = 10.48 DD122 pKa = 3.63 VLRR125 pKa = 11.84 FGLTNPHH132 pKa = 6.48 TCPQVDD138 pKa = 3.81 TACAVISDD146 pKa = 4.26 TLHH149 pKa = 6.45 FMSTHH154 pKa = 4.83 QVWTLFQNNPKK165 pKa = 10.35 LEE167 pKa = 4.08 RR168 pKa = 11.84 LYY170 pKa = 10.23 GTLVLPVEE178 pKa = 4.86 ALHH181 pKa = 6.49 KK182 pKa = 10.57 LPSLFPEE189 pKa = 4.6 IYY191 pKa = 9.95 KK192 pKa = 10.38 LEE194 pKa = 4.14 YY195 pKa = 9.81 YY196 pKa = 9.52 EE197 pKa = 4.72 RR198 pKa = 11.84 HH199 pKa = 5.31 FAYY202 pKa = 9.93 MPGGYY207 pKa = 10.15 GGGAYY212 pKa = 8.77 IHH214 pKa = 7.09 SYY216 pKa = 8.85 GTLKK220 pKa = 10.24 WLSTAQIGPGAYY232 pKa = 9.03 PGYY235 pKa = 9.94 RR236 pKa = 11.84 PPANGRR242 pKa = 11.84 RR243 pKa = 11.84 PLLDD247 pKa = 3.43 HH248 pKa = 7.0 LSIEE252 pKa = 4.43 KK253 pKa = 10.26 IEE255 pKa = 4.33 TKK257 pKa = 10.09 AAHH260 pKa = 6.81 HH261 pKa = 6.24 IVMIQRR267 pKa = 11.84 RR268 pKa = 11.84 RR269 pKa = 11.84 PEE271 pKa = 4.54 LPWPLPPIWVFHH283 pKa = 6.41 ASDD286 pKa = 4.04 YY287 pKa = 11.35 VKK289 pKa = 10.55 LPEE292 pKa = 3.88 IFYY295 pKa = 9.44 PAEE298 pKa = 4.09 ANVQKK303 pKa = 10.61 AYY305 pKa = 9.7 PHH307 pKa = 5.81 TFIKK311 pKa = 10.5 RR312 pKa = 11.84 LQLYY316 pKa = 8.31 CFSVKK321 pKa = 10.2 AVSLRR326 pKa = 11.84 DD327 pKa = 3.18 IFAKK331 pKa = 9.72 IRR333 pKa = 11.84 QVIPTEE339 pKa = 3.68 EE340 pKa = 4.04 LARR343 pKa = 11.84 FSTADD348 pKa = 4.67 LIRR351 pKa = 11.84 LANYY355 pKa = 10.44 LLFITGMQSASDD367 pKa = 3.9 YY368 pKa = 10.64 QSPLMEE374 pKa = 4.71 SLLGKK379 pKa = 10.15 LCLSIRR385 pKa = 11.84 ARR387 pKa = 11.84 VRR389 pKa = 11.84 EE390 pKa = 4.06 FFQNILGKK398 pKa = 10.11 SSYY401 pKa = 10.85 AALLTVTDD409 pKa = 3.44 VRR411 pKa = 11.84 PIHH414 pKa = 6.0 FTTKK418 pKa = 7.78 PQRR421 pKa = 11.84 RR422 pKa = 11.84 GICGEE427 pKa = 3.78 QWFDD431 pKa = 3.84 NPEE434 pKa = 3.8 EE435 pKa = 4.9 DD436 pKa = 5.0 EE437 pKa = 5.1 AGPPNNSLTGYY448 pKa = 6.96 PQPSPDD454 pKa = 4.98 DD455 pKa = 4.17 LDD457 pKa = 4.22 LLAEE461 pKa = 4.37 FEE463 pKa = 4.31 GHH465 pKa = 6.71 AMNPKK470 pKa = 8.83 TLSNNTRR477 pKa = 11.84 GRR479 pKa = 11.84 NDD481 pKa = 3.31 FPEE484 pKa = 4.99 CPVADD489 pKa = 3.91 TTAMNEE495 pKa = 3.56 EE496 pKa = 4.31 DD497 pKa = 3.61 TRR499 pKa = 11.84 RR500 pKa = 11.84 SHH502 pKa = 7.17 YY503 pKa = 10.21 EE504 pKa = 3.47 EE505 pKa = 4.7 HH506 pKa = 6.94 AKK508 pKa = 10.03 QATTTPNTEE517 pKa = 3.98 APKK520 pKa = 10.43 EE521 pKa = 3.95 ALTSDD526 pKa = 3.22 QEE528 pKa = 5.06 GYY530 pKa = 8.1 VTDD533 pKa = 3.77 WSDD536 pKa = 3.91 DD537 pKa = 3.68 EE538 pKa = 5.17 SSSSVKK544 pKa = 10.34 PPEE547 pKa = 4.34 SEE549 pKa = 4.11 GAPLEE554 pKa = 4.88 DD555 pKa = 5.88 DD556 pKa = 5.34 DD557 pKa = 6.9 LFDD560 pKa = 5.66 LPPLEE565 pKa = 5.53 DD566 pKa = 3.59 CADD569 pKa = 3.77 PVEE572 pKa = 5.61 ADD574 pKa = 3.55 AQEE577 pKa = 4.74 HH578 pKa = 6.39 PDD580 pKa = 3.8 EE581 pKa = 4.88 VSVDD585 pKa = 3.97 LPSPDD590 pKa = 3.61 SDD592 pKa = 3.59 HH593 pKa = 6.72 AHH595 pKa = 5.56 TGEE598 pKa = 4.22 RR599 pKa = 11.84 TLEE602 pKa = 4.07 TPEE605 pKa = 4.89 DD606 pKa = 4.22 SVTGEE611 pKa = 4.12 AQNCDD616 pKa = 3.13 GSRR619 pKa = 11.84 HH620 pKa = 5.18 IDD622 pKa = 3.44 EE623 pKa = 4.89 PEE625 pKa = 3.74 EE626 pKa = 3.92 RR627 pKa = 11.84 GQPSKK632 pKa = 9.41 QTCKK636 pKa = 10.82 VEE638 pKa = 4.0 LTPLDD643 pKa = 4.09 ADD645 pKa = 3.84 TVEE648 pKa = 4.06 LLKK651 pKa = 11.2 LHH653 pKa = 6.74 GFTNYY658 pKa = 9.23 RR659 pKa = 11.84 AQYY662 pKa = 9.98 DD663 pKa = 4.29 GEE665 pKa = 4.37 SQIQPIYY672 pKa = 10.1 RR673 pKa = 11.84 NPHH676 pKa = 4.15 AHH678 pKa = 7.48 FLRR681 pKa = 11.84 QYY683 pKa = 10.24 DD684 pKa = 3.83 AQTTTHH690 pKa = 5.3 QQQFVDD696 pKa = 3.71 MAHH699 pKa = 5.79 GLKK702 pKa = 10.05 RR703 pKa = 11.84 RR704 pKa = 11.84 CYY706 pKa = 10.04 GYY708 pKa = 10.91 KK709 pKa = 9.94 VDD711 pKa = 4.93 AKK713 pKa = 10.44 RR714 pKa = 11.84 ATAFMSDD721 pKa = 3.27 VKK723 pKa = 11.38 NNLTGLVLPKK733 pKa = 10.1 LDD735 pKa = 4.65 RR736 pKa = 11.84 DD737 pKa = 4.19 LLSSWVALAEE747 pKa = 4.14 NATRR751 pKa = 11.84 TVGLIVIHH759 pKa = 6.17 GAGGAGKK766 pKa = 10.07 SRR768 pKa = 11.84 ALQEE772 pKa = 3.92 LLRR775 pKa = 11.84 NRR777 pKa = 11.84 VVDD780 pKa = 4.01 ANEE783 pKa = 3.77 VNIVVPTINLAADD796 pKa = 3.71 WKK798 pKa = 10.58 KK799 pKa = 10.77 KK800 pKa = 10.55 LPALDD805 pKa = 3.76 PRR807 pKa = 11.84 RR808 pKa = 11.84 VMTFEE813 pKa = 4.09 KK814 pKa = 10.45 ACEE817 pKa = 3.99 RR818 pKa = 11.84 EE819 pKa = 4.42 GKK821 pKa = 10.11 SVVIMDD827 pKa = 5.34 DD828 pKa = 3.58 YY829 pKa = 11.94 GKK831 pKa = 10.9 LPAGYY836 pKa = 9.92 VDD838 pKa = 5.04 AYY840 pKa = 11.11 LAIKK844 pKa = 10.64 SNVEE848 pKa = 4.21 LIVLTGDD855 pKa = 3.3 QRR857 pKa = 11.84 QSVYY861 pKa = 11.02 HH862 pKa = 5.98 NDD864 pKa = 3.26 KK865 pKa = 10.49 RR866 pKa = 11.84 DD867 pKa = 3.5 SQIALLSPNTEE878 pKa = 3.8 HH879 pKa = 7.44 FKK881 pKa = 10.58 RR882 pKa = 11.84 YY883 pKa = 9.09 CDD885 pKa = 3.63 YY886 pKa = 11.06 YY887 pKa = 11.31 VNATHH892 pKa = 6.72 RR893 pKa = 11.84 QPRR896 pKa = 11.84 RR897 pKa = 11.84 LANPIRR903 pKa = 11.84 VHH905 pKa = 6.64 AEE907 pKa = 3.84 RR908 pKa = 11.84 EE909 pKa = 3.82 VGGAVKK915 pKa = 10.02 HH916 pKa = 5.55 ATLIPTNAMTLVPAFRR932 pKa = 11.84 SQSLLTDD939 pKa = 4.09 LGRR942 pKa = 11.84 QAMTYY947 pKa = 10.18 AGCQGLTLPHH957 pKa = 6.01 LTIVLDD963 pKa = 4.36 KK964 pKa = 10.04 DD965 pKa = 3.68 TPLCSDD971 pKa = 3.5 EE972 pKa = 4.38 VLYY975 pKa = 9.62 TALSRR980 pKa = 11.84 ASEE983 pKa = 4.34 TITFVNTHH991 pKa = 5.86 SDD993 pKa = 3.18 NKK995 pKa = 10.56 EE996 pKa = 3.68 FLAKK1000 pKa = 10.27 LDD1002 pKa = 3.46 ATPYY1006 pKa = 10.68 LKK1008 pKa = 9.78 TLISGVRR1015 pKa = 11.84 EE1016 pKa = 4.11 DD1017 pKa = 3.91 EE1018 pKa = 4.22 QSGGDD1023 pKa = 3.79 YY1024 pKa = 10.45 QPPEE1028 pKa = 3.88 PQVRR1032 pKa = 11.84 DD1033 pKa = 3.95 GPTKK1037 pKa = 9.4 THH1039 pKa = 6.14 MPVANNNIQLEE1050 pKa = 4.44 EE1051 pKa = 4.88 KK1052 pKa = 10.35 IEE1054 pKa = 4.04 TMEE1057 pKa = 5.18 DD1058 pKa = 3.12 KK1059 pKa = 10.11 DD1060 pKa = 4.63 TRR1062 pKa = 11.84 EE1063 pKa = 3.87 LWSGEE1068 pKa = 3.84 EE1069 pKa = 4.18 KK1070 pKa = 10.4 TNLMQTQDD1078 pKa = 3.55 PVVQLFPHH1086 pKa = 5.42 QQSKK1090 pKa = 11.5 DD1091 pKa = 3.3 EE1092 pKa = 4.42 ALFKK1096 pKa = 10.16 ITIKK1100 pKa = 10.57 EE1101 pKa = 4.23 RR1102 pKa = 11.84 IRR1104 pKa = 11.84 TADD1107 pKa = 2.85 VGTNKK1112 pKa = 10.11 KK1113 pKa = 9.88 ALCEE1117 pKa = 3.9 TLSAGDD1123 pKa = 4.57 LLFEE1127 pKa = 5.24 AYY1129 pKa = 10.06 AKK1131 pKa = 10.37 FMNVPQEE1138 pKa = 4.0 TQPFDD1143 pKa = 3.35 KK1144 pKa = 10.7 RR1145 pKa = 11.84 LWAHH1149 pKa = 6.7 CKK1151 pKa = 9.57 QLALRR1156 pKa = 11.84 TYY1158 pKa = 10.57 LSKK1161 pKa = 9.75 PTTNLQQGAQRR1172 pKa = 11.84 QDD1174 pKa = 2.93 PDD1176 pKa = 3.87 FPDD1179 pKa = 3.11 NAIALFNKK1187 pKa = 8.34 SQWVKK1192 pKa = 10.35 KK1193 pKa = 10.25 LEE1195 pKa = 4.06 KK1196 pKa = 10.47 VGCKK1200 pKa = 9.95 FKK1202 pKa = 11.02 AGQTISAFKK1211 pKa = 10.55 QSTVLLTTTMALYY1224 pKa = 10.13 LRR1226 pKa = 11.84 KK1227 pKa = 9.51 KK1228 pKa = 10.63 RR1229 pKa = 11.84 EE1230 pKa = 3.53 GHH1232 pKa = 5.43 QPDD1235 pKa = 3.93 NVFVMCEE1242 pKa = 3.82 KK1243 pKa = 10.29 TPEE1246 pKa = 4.06 QFDD1249 pKa = 4.07 SFVLTKK1255 pKa = 9.99 WDD1257 pKa = 4.4 FSRR1260 pKa = 11.84 PNYY1263 pKa = 9.07 TSDD1266 pKa = 3.01 YY1267 pKa = 8.03 TQYY1270 pKa = 11.28 DD1271 pKa = 3.62 QSQDD1275 pKa = 3.09 AAFLNFEE1282 pKa = 4.32 LRR1284 pKa = 11.84 KK1285 pKa = 9.67 ARR1287 pKa = 11.84 HH1288 pKa = 5.43 FGIPNEE1294 pKa = 4.21 VVDD1297 pKa = 6.08 FYY1299 pKa = 12.1 AFIKK1303 pKa = 8.86 THH1305 pKa = 6.42 AKK1307 pKa = 9.19 TFLGNLAIMRR1317 pKa = 11.84 LSGEE1321 pKa = 4.31 GPTFDD1326 pKa = 6.1 ANTEE1330 pKa = 4.05 CNIAYY1335 pKa = 8.27 DD1336 pKa = 3.69 ALRR1339 pKa = 11.84 FQVDD1343 pKa = 3.65 STVNACYY1350 pKa = 10.3 AGDD1353 pKa = 3.77 DD1354 pKa = 3.85 LVRR1357 pKa = 11.84 DD1358 pKa = 4.11 KK1359 pKa = 11.41 ACEE1362 pKa = 3.73 EE1363 pKa = 4.09 RR1364 pKa = 11.84 PGWKK1368 pKa = 9.61 YY1369 pKa = 11.18 AEE1371 pKa = 4.31 PLFSLKK1377 pKa = 10.56 AKK1379 pKa = 10.13 PLVTNKK1385 pKa = 9.9 PDD1387 pKa = 3.19 FCGWRR1392 pKa = 11.84 LTKK1395 pKa = 10.67 FGIVKK1400 pKa = 10.5 SPVQLYY1406 pKa = 10.28 QSLQLALRR1414 pKa = 11.84 LGKK1417 pKa = 10.19 VEE1419 pKa = 4.03 EE1420 pKa = 4.42 VKK1422 pKa = 10.49 RR1423 pKa = 11.84 SYY1425 pKa = 11.23 AIDD1428 pKa = 3.38 YY1429 pKa = 9.93 LFAYY1433 pKa = 10.43 RR1434 pKa = 11.84 LGDD1437 pKa = 3.26 QLYY1440 pKa = 10.87 DD1441 pKa = 3.48 VFDD1444 pKa = 4.15 EE1445 pKa = 4.8 NEE1447 pKa = 4.12 MEE1449 pKa = 4.04 KK1450 pKa = 10.33 HH1451 pKa = 5.01 QLVTRR1456 pKa = 11.84 TLIKK1460 pKa = 10.4 KK1461 pKa = 9.38 GMRR1464 pKa = 11.84 PPSSGDD1470 pKa = 3.29 HH1471 pKa = 6.27 LPVFHH1476 pKa = 6.29 VTSDD1480 pKa = 3.09 RR1481 pKa = 11.84 LIRR1484 pKa = 11.84 DD1485 pKa = 4.04 PKK1487 pKa = 10.22 AKK1489 pKa = 10.21 HH1490 pKa = 5.61 ISTYY1494 pKa = 10.26 EE1495 pKa = 3.88 YY1496 pKa = 10.7 DD1497 pKa = 3.9 STSLPFNIVEE1507 pKa = 4.47 DD1508 pKa = 3.72 HH1509 pKa = 6.64 FAYY1512 pKa = 10.62 NPNRR1516 pKa = 11.84 ADD1518 pKa = 4.09 RR1519 pKa = 11.84 DD1520 pKa = 3.97 DD1521 pKa = 3.77 MNMARR1526 pKa = 11.84 DD1527 pKa = 4.03 SQSHH1531 pKa = 4.56 EE1532 pKa = 3.98 TSNASNFPPARR1543 pKa = 11.84 EE1544 pKa = 4.08 PLLSDD1549 pKa = 4.9 LFPQLSSLL1557 pKa = 3.93
Molecular weight: 175.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.694
IPC2_protein 5.626
IPC_protein 5.69
Toseland 6.084
ProMoST 6.033
Dawson 5.931
Bjellqvist 5.957
Wikipedia 5.931
Rodwell 5.919
Grimsley 6.237
Solomon 5.931
Lehninger 5.919
Nozaki 6.198
DTASelect 6.389
Thurlkill 6.389
EMBOSS 6.351
Sillero 6.287
Patrickios 4.126
IPC_peptide 5.944
IPC2_peptide 6.287
IPC2.peptide.svr19 6.238
Protein with the highest isoelectric point:
>tr|Q8QXV9|Q8QXV9_9VIRU Coat protein OS=Garlic virus E OX=150285 PE=4 SV=1
MM1 pKa = 7.51 HH2 pKa = 7.32 PHH4 pKa = 7.33 DD5 pKa = 5.95 FNLLCCLHH13 pKa = 6.73 FSPNHH18 pKa = 5.86 LPKK21 pKa = 10.63 DD22 pKa = 3.61 LQILIFSRR30 pKa = 11.84 VTTEE34 pKa = 3.62 CKK36 pKa = 9.88 LRR38 pKa = 11.84 RR39 pKa = 11.84 LSEE42 pKa = 4.03 NKK44 pKa = 9.53 PFRR47 pKa = 11.84 GRR49 pKa = 11.84 SKK51 pKa = 9.6 FAMRR55 pKa = 11.84 RR56 pKa = 11.84 RR57 pKa = 11.84 AKK59 pKa = 10.21 RR60 pKa = 11.84 YY61 pKa = 8.54 NRR63 pKa = 11.84 CFDD66 pKa = 3.9 CGAVLIDD73 pKa = 3.56 DD74 pKa = 4.57 HH75 pKa = 5.77 QCKK78 pKa = 10.21 VLTSRR83 pKa = 11.84 AQSDD87 pKa = 4.15 VLTVIRR93 pKa = 11.84 EE94 pKa = 4.5 GPAKK98 pKa = 10.35 LYY100 pKa = 10.92 AEE102 pKa = 3.9 RR103 pKa = 11.84 TYY105 pKa = 11.12 RR106 pKa = 11.84 PNSDD110 pKa = 3.17 AARR113 pKa = 11.84 LIEE116 pKa = 6.11 DD117 pKa = 5.06 DD118 pKa = 3.12 ILYY121 pKa = 10.29 IKK123 pKa = 8.26 TLKK126 pKa = 10.55 LL127 pKa = 3.3
Molecular weight: 14.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.198
IPC2_protein 8.902
IPC_protein 8.916
Toseland 9.589
ProMoST 9.37
Dawson 9.853
Bjellqvist 9.648
Wikipedia 9.999
Rodwell 10.131
Grimsley 9.897
Solomon 9.911
Lehninger 9.882
Nozaki 9.867
DTASelect 9.56
Thurlkill 9.721
EMBOSS 10.028
Sillero 9.853
Patrickios 7.585
IPC_peptide 9.911
IPC2_peptide 8.726
IPC2.peptide.svr19 7.8
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
3226
103
1557
403.3
45.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.812 ± 0.434
1.984 ± 0.237
6.2 ± 0.452
5.177 ± 0.63
4.123 ± 0.29
5.053 ± 0.515
2.976 ± 0.305
4.061 ± 0.405
4.681 ± 0.676
9.454 ± 0.806
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.108 ± 0.477
4.96 ± 0.654
6.851 ± 0.322
4.805 ± 0.343
6.107 ± 0.411
7.502 ± 0.949
7.316 ± 0.307
5.084 ± 0.155
0.682 ± 0.12
3.069 ± 0.446
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here