Streptococcus satellite phage Javan543

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZQ38|A0A4D5ZQ38_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan543 OX=2558737 GN=JavanS543_0009 PE=4 SV=1
MM1 pKa = 7.79AEE3 pKa = 4.07KK4 pKa = 10.11TEE6 pKa = 4.08QTKK9 pKa = 8.2TVQLTVEE16 pKa = 4.19EE17 pKa = 4.45LQNLGCRR24 pKa = 11.84LSNILKK30 pKa = 8.24TIKK33 pKa = 9.45MDD35 pKa = 3.49QVAQAGVSLAKK46 pKa = 10.47EE47 pKa = 3.58RR48 pKa = 11.84DD49 pKa = 3.71LFTFTHH55 pKa = 7.19LATNYY60 pKa = 9.86LSSSYY65 pKa = 10.73EE66 pKa = 3.78VFEE69 pKa = 4.5TIIAEE74 pKa = 4.32LDD76 pKa = 4.33DD77 pKa = 4.17IASQLLEE84 pKa = 4.78CDD86 pKa = 3.5DD87 pKa = 4.89AEE89 pKa = 4.1EE90 pKa = 5.38LEE92 pKa = 4.18AFKK95 pKa = 10.99NGRR98 pKa = 3.53

Molecular weight: 11.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZV77|A0A4D5ZV77_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan543 OX=2558737 GN=JavanS543_0004 PE=4 SV=1
MM1 pKa = 7.88KK2 pKa = 9.33ITEE5 pKa = 4.22VKK7 pKa = 9.98KK8 pKa = 10.55KK9 pKa = 10.47NGTIVYY15 pKa = 8.89RR16 pKa = 11.84ASVYY20 pKa = 10.85LGVDD24 pKa = 2.66KK25 pKa = 11.05VTGKK29 pKa = 10.21KK30 pKa = 10.24VKK32 pKa = 9.81TSITGRR38 pKa = 11.84TRR40 pKa = 11.84KK41 pKa = 8.72EE42 pKa = 4.15VKK44 pKa = 10.33SKK46 pKa = 10.67AQQAQFDD53 pKa = 4.49FKK55 pKa = 11.64SNGSTVKK62 pKa = 9.6KK63 pKa = 8.03TVQVKK68 pKa = 8.4TYY70 pKa = 10.65KK71 pKa = 10.39EE72 pKa = 4.03LAALWLEE79 pKa = 4.23SHH81 pKa = 6.88KK82 pKa = 10.66LTVRR86 pKa = 11.84PQTFAVTRR94 pKa = 11.84RR95 pKa = 11.84QVNHH99 pKa = 6.25HH100 pKa = 7.38LIPEE104 pKa = 4.71FGSMKK109 pKa = 9.3IDD111 pKa = 4.12KK112 pKa = 8.35ITPSSIQEE120 pKa = 4.24FINNLAYY127 pKa = 9.9KK128 pKa = 9.92IVNFKK133 pKa = 10.17EE134 pKa = 3.78INSINRR140 pKa = 11.84RR141 pKa = 11.84ILQHH145 pKa = 6.43GVTLQLLASNPGRR158 pKa = 11.84DD159 pKa = 3.25VILPKK164 pKa = 10.02KK165 pKa = 8.7QKK167 pKa = 9.97KK168 pKa = 9.27GRR170 pKa = 11.84EE171 pKa = 3.9AVKK174 pKa = 10.5FIDD177 pKa = 4.6PDD179 pKa = 3.88DD180 pKa = 4.39LKK182 pKa = 11.13MFIDD186 pKa = 4.04YY187 pKa = 10.93AEE189 pKa = 4.33KK190 pKa = 10.79LSTKK194 pKa = 9.78SYY196 pKa = 7.56KK197 pKa = 9.42HH198 pKa = 5.68YY199 pKa = 10.78YY200 pKa = 9.1RR201 pKa = 11.84FVVFKK206 pKa = 10.82LLLATGCRR214 pKa = 11.84IGEE217 pKa = 4.1LAALEE222 pKa = 4.18WSDD225 pKa = 4.34IDD227 pKa = 5.1LKK229 pKa = 11.17EE230 pKa = 3.89KK231 pKa = 10.26TITISKK237 pKa = 9.01TYY239 pKa = 8.95SQEE242 pKa = 2.82IRR244 pKa = 11.84MIGEE248 pKa = 4.42TKK250 pKa = 9.99TKK252 pKa = 10.62AGTRR256 pKa = 11.84KK257 pKa = 9.66ISIDD261 pKa = 3.45SKK263 pKa = 8.84TALMLKK269 pKa = 9.9QYY271 pKa = 8.82QNRR274 pKa = 11.84QRR276 pKa = 11.84LMFLEE281 pKa = 4.79VGAQAPSVVFATTVRR296 pKa = 11.84EE297 pKa = 3.82YY298 pKa = 11.13LPRR301 pKa = 11.84HH302 pKa = 5.71SLQQIIDD309 pKa = 3.93KK310 pKa = 10.56RR311 pKa = 11.84CEE313 pKa = 3.8TLGIPRR319 pKa = 11.84FTCHH323 pKa = 6.9AFRR326 pKa = 11.84HH327 pKa = 4.86THH329 pKa = 7.04ASLLLNAGISYY340 pKa = 10.68KK341 pKa = 10.23EE342 pKa = 3.61LQYY345 pKa = 11.52RR346 pKa = 11.84LGHH349 pKa = 5.98SNISMTLDD357 pKa = 3.55VYY359 pKa = 11.5SHH361 pKa = 7.06LSKK364 pKa = 11.04DD365 pKa = 3.51KK366 pKa = 10.41EE367 pKa = 4.25KK368 pKa = 10.93EE369 pKa = 3.85AVSYY373 pKa = 9.5YY374 pKa = 10.62EE375 pKa = 4.12KK376 pKa = 10.7AINSLL381 pKa = 3.57

Molecular weight: 43.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18

0

18

2970

39

546

165.0

19.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.633 ± 0.667

0.741 ± 0.169

5.657 ± 0.449

8.215 ± 0.712

4.242 ± 0.309

4.949 ± 0.506

1.38 ± 0.314

7.407 ± 0.561

10.471 ± 0.955

9.899 ± 0.55

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.29 ± 0.287

4.882 ± 0.454

2.862 ± 0.454

4.512 ± 0.417

4.983 ± 0.389

5.354 ± 0.388

5.589 ± 0.5

4.68 ± 0.412

0.943 ± 0.118

4.31 ± 0.393
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski