Escherichia phage CJ20

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Gaprivervirus; unclassified Gaprivervirus

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 307 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D7EMV2|A0A7D7EMV2_9CAUD Uncharacterized protein OS=Escherichia phage CJ20 OX=2744001 GN=CJ20_057 PE=4 SV=1
MM1 pKa = 7.92LIMMKK6 pKa = 9.66MYY8 pKa = 10.57KK9 pKa = 9.6HH10 pKa = 6.88DD11 pKa = 3.66FHH13 pKa = 9.27IGDD16 pKa = 3.28GWFGNVEE23 pKa = 3.93WDD25 pKa = 4.5VDD27 pKa = 4.04GSWYY31 pKa = 9.23TGTMYY36 pKa = 9.56VTSEE40 pKa = 3.99YY41 pKa = 11.05SEE43 pKa = 4.26GVSWAMEE50 pKa = 4.23LDD52 pKa = 3.77DD53 pKa = 4.94VFASSEE59 pKa = 3.92EE60 pKa = 3.66MLEE63 pKa = 4.05YY64 pKa = 10.74VEE66 pKa = 4.22QTIRR70 pKa = 11.84EE71 pKa = 4.25NNTT74 pKa = 2.83

Molecular weight: 8.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D7ISK5|A0A7D7ISK5_9CAUD Uncharacterized protein OS=Escherichia phage CJ20 OX=2744001 GN=CJ20_251 PE=4 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84PFFYY6 pKa = 10.94SLINAIQTCLNVSLTFVISCFLTSTGFSDD35 pKa = 3.72WVNSRR40 pKa = 11.84PSRR43 pKa = 11.84PASSRR48 pKa = 11.84ISWTVKK54 pKa = 9.94PITFPKK60 pKa = 10.52SFGDD64 pKa = 3.42TSPILLITLLIRR76 pKa = 11.84LTVTT80 pKa = 3.44

Molecular weight: 8.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

307

0

307

58002

74

1310

188.9

21.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.946 ± 0.153

1.274 ± 0.084

5.877 ± 0.111

6.564 ± 0.174

4.776 ± 0.137

5.974 ± 0.231

1.89 ± 0.081

7.108 ± 0.121

7.155 ± 0.214

7.801 ± 0.154

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.957 ± 0.102

5.543 ± 0.16

3.721 ± 0.1

3.691 ± 0.101

4.407 ± 0.096

6.362 ± 0.126

5.96 ± 0.205

6.596 ± 0.119

1.359 ± 0.058

4.04 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski