Salmonella virus PsP3
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6K1H5|Q6K1H5_9CAUD Gp16 OS=Salmonella virus PsP3 OX=12407 PE=4 SV=1
MM1 pKa = 7.23 ATVDD5 pKa = 4.28 LSQLPVPDD13 pKa = 3.55 VVEE16 pKa = 4.06 EE17 pKa = 4.11 LDD19 pKa = 3.72 YY20 pKa = 10.57 EE21 pKa = 4.61 TILAEE26 pKa = 5.12 RR27 pKa = 11.84 IATLISLYY35 pKa = 10.45 PEE37 pKa = 4.58 DD38 pKa = 3.68 QQEE41 pKa = 4.43 AIARR45 pKa = 11.84 TLALEE50 pKa = 4.07 SEE52 pKa = 5.07 PIVKK56 pKa = 10.22 LLQEE60 pKa = 3.72 NAYY63 pKa = 10.54 RR64 pKa = 11.84 EE65 pKa = 4.54 VIWRR69 pKa = 11.84 QRR71 pKa = 11.84 VNEE74 pKa = 3.96 AARR77 pKa = 11.84 AVMLAYY83 pKa = 10.24 AIDD86 pKa = 4.06 SDD88 pKa = 4.99 LDD90 pKa = 4.02 NIGANFNVEE99 pKa = 4.12 RR100 pKa = 11.84 LVVTPADD107 pKa = 3.8 DD108 pKa = 3.53 TTIPHH113 pKa = 6.57 TPAEE117 pKa = 4.31 MEE119 pKa = 3.92 LDD121 pKa = 2.96 ADD123 pKa = 3.62 YY124 pKa = 11.22 RR125 pKa = 11.84 LRR127 pKa = 11.84 IQQAFEE133 pKa = 4.04 GLSVAGSVGSYY144 pKa = 9.74 QYY146 pKa = 10.55 HH147 pKa = 5.94 GRR149 pKa = 11.84 SADD152 pKa = 3.24 GRR154 pKa = 11.84 VGDD157 pKa = 4.33 ISVISPSPACVTISVLSRR175 pKa = 11.84 EE176 pKa = 4.35 NNGVASEE183 pKa = 4.1 EE184 pKa = 3.82 LLAIVRR190 pKa = 11.84 NALNAEE196 pKa = 4.28 DD197 pKa = 4.08 VRR199 pKa = 11.84 PVADD203 pKa = 3.2 RR204 pKa = 11.84 VTVQSAEE211 pKa = 3.77 IVNYY215 pKa = 9.8 QINATLYY222 pKa = 9.71 LYY224 pKa = 8.88 PGPEE228 pKa = 4.09 SEE230 pKa = 5.15 PIRR233 pKa = 11.84 AAAEE237 pKa = 4.21 AKK239 pKa = 10.05 LKK241 pKa = 10.73 AYY243 pKa = 9.88 ISAQHH248 pKa = 6.43 RR249 pKa = 11.84 LGRR252 pKa = 11.84 DD253 pKa = 2.91 IRR255 pKa = 11.84 KK256 pKa = 8.91 SAIYY260 pKa = 9.91 AALHH264 pKa = 5.3 VEE266 pKa = 4.81 GVQRR270 pKa = 11.84 VEE272 pKa = 3.97 LAAPVADD279 pKa = 2.95 IVLDD283 pKa = 3.5 NTQASFCTDD292 pKa = 3.12 YY293 pKa = 11.51 SLVIGGSDD301 pKa = 3.28 EE302 pKa = 3.99
Molecular weight: 32.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.444
IPC2_protein 4.444
IPC_protein 4.38
Toseland 4.215
ProMoST 4.457
Dawson 4.329
Bjellqvist 4.495
Wikipedia 4.202
Rodwell 4.215
Grimsley 4.126
Solomon 4.329
Lehninger 4.279
Nozaki 4.431
DTASelect 4.596
Thurlkill 4.228
EMBOSS 4.215
Sillero 4.495
Patrickios 3.63
IPC_peptide 4.329
IPC2_peptide 4.482
IPC2.peptide.svr19 4.42
Protein with the highest isoelectric point:
>tr|Q6K1I6|Q6K1I6_9CAUD Gp5 OS=Salmonella virus PsP3 OX=12407 PE=4 SV=1
MM1 pKa = 7.46 TSPAQRR7 pKa = 11.84 HH8 pKa = 4.75 MMRR11 pKa = 11.84 VSASQAAQRR20 pKa = 11.84 EE21 pKa = 4.41 QAPLRR26 pKa = 11.84 HH27 pKa = 5.15 ATAYY31 pKa = 10.23 EE32 pKa = 3.82 QMLVKK37 pKa = 10.54 LADD40 pKa = 3.75 DD41 pKa = 3.86 RR42 pKa = 11.84 RR43 pKa = 11.84 TLKK46 pKa = 10.64 NIRR49 pKa = 11.84 SNEE52 pKa = 3.34 RR53 pKa = 11.84 KK54 pKa = 9.19 AAKK57 pKa = 9.76 KK58 pKa = 10.31 RR59 pKa = 11.84 EE60 pKa = 4.19 LLPFYY65 pKa = 10.02 APWVAGVLADD75 pKa = 4.68 GRR77 pKa = 11.84 GAQDD81 pKa = 4.55 DD82 pKa = 3.64 IVMTVMLWRR91 pKa = 11.84 LDD93 pKa = 3.59 AGDD96 pKa = 3.31 IAGALEE102 pKa = 3.88 IAPYY106 pKa = 10.18 ALQYY110 pKa = 11.72 GLTTDD115 pKa = 3.39 HH116 pKa = 7.04 RR117 pKa = 11.84 RR118 pKa = 11.84 TTPYY122 pKa = 10.08 MLVEE126 pKa = 4.47 EE127 pKa = 4.52 VALAALRR134 pKa = 11.84 LRR136 pKa = 11.84 DD137 pKa = 3.6 AGEE140 pKa = 4.19 SVDD143 pKa = 5.46 LSWLQTTIDD152 pKa = 3.98 LTDD155 pKa = 3.98 GADD158 pKa = 3.46 VPDD161 pKa = 3.8 MVRR164 pKa = 11.84 ARR166 pKa = 11.84 LHH168 pKa = 6.13 KK169 pKa = 10.05 VTGLTLRR176 pKa = 11.84 DD177 pKa = 3.02 AGMNTEE183 pKa = 5.11 ALAQFQRR190 pKa = 11.84 AMQLDD195 pKa = 4.43 RR196 pKa = 11.84 NTGVRR201 pKa = 11.84 KK202 pKa = 9.72 EE203 pKa = 3.85 IEE205 pKa = 3.92 RR206 pKa = 11.84 LEE208 pKa = 4.2 RR209 pKa = 11.84 ALKK212 pKa = 10.02 PKK214 pKa = 10.83 AEE216 pKa = 4.15 APPRR220 pKa = 11.84 KK221 pKa = 5.87 TTKK224 pKa = 9.66 PRR226 pKa = 11.84 TRR228 pKa = 11.84 KK229 pKa = 9.09 PVARR233 pKa = 11.84 PAAKK237 pKa = 9.8 RR238 pKa = 11.84 GRR240 pKa = 11.84 PSKK243 pKa = 10.52 AVKK246 pKa = 8.67 TAGG249 pKa = 3.25
Molecular weight: 27.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.257
IPC2_protein 9.487
IPC_protein 9.926
Toseland 10.57
ProMoST 10.16
Dawson 10.657
Bjellqvist 10.306
Wikipedia 10.818
Rodwell 10.95
Grimsley 10.701
Solomon 10.745
Lehninger 10.716
Nozaki 10.526
DTASelect 10.306
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.57
Patrickios 10.657
IPC_peptide 10.745
IPC2_peptide 8.843
IPC2.peptide.svr19 8.797
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
42
0
42
9518
60
814
226.6
25.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.454 ± 0.536
1.009 ± 0.154
6.146 ± 0.28
5.936 ± 0.353
3.162 ± 0.276
6.987 ± 0.42
1.923 ± 0.195
5.358 ± 0.212
5.61 ± 0.309
9.246 ± 0.299
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.522 ± 0.152
4.097 ± 0.269
4.087 ± 0.238
3.929 ± 0.291
6.251 ± 0.414
6.104 ± 0.289
6.567 ± 0.384
6.503 ± 0.305
1.565 ± 0.164
2.543 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here