Methylacidiphilum fumariolicum (strain SolV)
Average proteome isoelectric point is 7.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2208 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I0JWC0|I0JWC0_METFB Uncharacterized protein OS=Methylacidiphilum fumariolicum (strain SolV) OX=1156937 GN=MFUM_130003 PE=4 SV=1
MM1 pKa = 7.79 GSPRR5 pKa = 11.84 WEE7 pKa = 4.19 STDD10 pKa = 3.22 EE11 pKa = 4.04 LVEE14 pKa = 4.21 VEE16 pKa = 6.55 AMLQLDD22 pKa = 4.12 NFGPCPGYY30 pKa = 10.67 HH31 pKa = 6.75 CLSTADD37 pKa = 4.83 CSSQQPSLAEE47 pKa = 3.73 GWIADD52 pKa = 3.8 TT53 pKa = 5.16
Molecular weight: 5.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.88
IPC2_protein 3.808
IPC_protein 3.617
Toseland 3.465
ProMoST 3.821
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.579
Rodwell 3.49
Grimsley 3.401
Solomon 3.567
Lehninger 3.528
Nozaki 3.783
DTASelect 3.897
Thurlkill 3.554
EMBOSS 3.592
Sillero 3.757
Patrickios 0.172
IPC_peptide 3.567
IPC2_peptide 3.719
IPC2.peptide.svr19 3.701
Protein with the highest isoelectric point:
>tr|I0JW96|I0JW96_METFB Pseudouridine synthase OS=Methylacidiphilum fumariolicum (strain SolV) OX=1156937 GN=rluA PE=3 SV=1
MM1 pKa = 7.61 HH2 pKa = 7.39 SLNLSRR8 pKa = 11.84 KK9 pKa = 7.97 MVSLQKK15 pKa = 10.36 AARR18 pKa = 11.84 VPEE21 pKa = 4.59 GFCPNAAVMGTRR33 pKa = 11.84 GQAFAASPHH42 pKa = 6.27 GGRR45 pKa = 11.84 TPALRR50 pKa = 11.84 SCAVAPEE57 pKa = 4.35 LFRR60 pKa = 11.84 AEE62 pKa = 5.43 AEE64 pKa = 4.41 ANWRR68 pKa = 11.84 TIAYY72 pKa = 9.63 ARR74 pKa = 11.84 VSSPDD79 pKa = 2.95
Molecular weight: 8.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.355
IPC_protein 10.072
Toseland 10.409
ProMoST 10.116
Dawson 10.526
Bjellqvist 10.277
Wikipedia 10.73
Rodwell 10.643
Grimsley 10.57
Solomon 10.657
Lehninger 10.643
Nozaki 10.467
DTASelect 10.248
Thurlkill 10.423
EMBOSS 10.818
Sillero 10.482
Patrickios 10.54
IPC_peptide 10.672
IPC2_peptide 9.648
IPC2.peptide.svr19 8.386
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2208
0
2208
659916
31
1834
298.9
33.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.887 ± 0.049
1.228 ± 0.017
4.212 ± 0.033
7.085 ± 0.051
5.283 ± 0.047
6.802 ± 0.042
2.062 ± 0.025
7.488 ± 0.045
6.963 ± 0.047
11.028 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.06 ± 0.023
3.713 ± 0.036
4.925 ± 0.036
3.611 ± 0.031
4.872 ± 0.044
6.837 ± 0.042
4.36 ± 0.032
5.885 ± 0.044
1.494 ± 0.024
3.206 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here