Methylacidiphilum fumariolicum (strain SolV)

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Methylacidiphilae; Methylacidiphilales; Methylacidiphilaceae; Methylacidiphilum; Methylacidiphilum fumariolicum

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2208 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I0JWC0|I0JWC0_METFB Uncharacterized protein OS=Methylacidiphilum fumariolicum (strain SolV) OX=1156937 GN=MFUM_130003 PE=4 SV=1
MM1 pKa = 7.79GSPRR5 pKa = 11.84WEE7 pKa = 4.19STDD10 pKa = 3.22EE11 pKa = 4.04LVEE14 pKa = 4.21VEE16 pKa = 6.55AMLQLDD22 pKa = 4.12NFGPCPGYY30 pKa = 10.67HH31 pKa = 6.75CLSTADD37 pKa = 4.83CSSQQPSLAEE47 pKa = 3.73GWIADD52 pKa = 3.8TT53 pKa = 5.16

Molecular weight: 5.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I0JW96|I0JW96_METFB Pseudouridine synthase OS=Methylacidiphilum fumariolicum (strain SolV) OX=1156937 GN=rluA PE=3 SV=1
MM1 pKa = 7.61HH2 pKa = 7.39SLNLSRR8 pKa = 11.84KK9 pKa = 7.97MVSLQKK15 pKa = 10.36AARR18 pKa = 11.84VPEE21 pKa = 4.59GFCPNAAVMGTRR33 pKa = 11.84GQAFAASPHH42 pKa = 6.27GGRR45 pKa = 11.84TPALRR50 pKa = 11.84SCAVAPEE57 pKa = 4.35LFRR60 pKa = 11.84AEE62 pKa = 5.43AEE64 pKa = 4.41ANWRR68 pKa = 11.84TIAYY72 pKa = 9.63ARR74 pKa = 11.84VSSPDD79 pKa = 2.95

Molecular weight: 8.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2208

0

2208

659916

31

1834

298.9

33.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.887 ± 0.049

1.228 ± 0.017

4.212 ± 0.033

7.085 ± 0.051

5.283 ± 0.047

6.802 ± 0.042

2.062 ± 0.025

7.488 ± 0.045

6.963 ± 0.047

11.028 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.06 ± 0.023

3.713 ± 0.036

4.925 ± 0.036

3.611 ± 0.031

4.872 ± 0.044

6.837 ± 0.042

4.36 ± 0.032

5.885 ± 0.044

1.494 ± 0.024

3.206 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski