Drechslerella stenobrocha 248
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5595 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W7HK99|W7HK99_9PEZI Uncharacterized protein OS=Drechslerella stenobrocha 248 OX=1043628 GN=DRE_06819 PE=4 SV=1
MM1 pKa = 7.53 HH2 pKa = 7.25 PTKK5 pKa = 9.96 THH7 pKa = 7.1 LLPPLAALLLLRR19 pKa = 11.84 LAAAYY24 pKa = 8.64 EE25 pKa = 4.44 VGWGTWDD32 pKa = 3.73 EE33 pKa = 4.1 LTQINYY39 pKa = 9.8 NPVPATTDD47 pKa = 3.65 FANPPCHH54 pKa = 6.36 QIPFPASDD62 pKa = 3.21 VMEE65 pKa = 4.68 IYY67 pKa = 10.75 LRR69 pKa = 11.84 ASYY72 pKa = 10.69 ALEE75 pKa = 4.49 DD76 pKa = 4.63 APPPPYY82 pKa = 9.71 IAMYY86 pKa = 10.64 GSTDD90 pKa = 3.52 PAAQGCVDD98 pKa = 4.16 ASLLTLYY105 pKa = 10.97 AFDD108 pKa = 3.99 PTALDD113 pKa = 3.46 KK114 pKa = 11.0 MQGAWIVSPGPRR126 pKa = 11.84 YY127 pKa = 8.9 WRR129 pKa = 11.84 EE130 pKa = 3.84 LYY132 pKa = 9.83 PGQVQDD138 pKa = 4.7 PLAKK142 pKa = 10.36 ALLSSFPPFQIGDD155 pKa = 3.31 ILMKK159 pKa = 10.35 IEE161 pKa = 4.87 APPQAGAGYY170 pKa = 11.0 AWMKK174 pKa = 9.6 SQNKK178 pKa = 8.51 VFVEE182 pKa = 4.29 DD183 pKa = 3.44 WDD185 pKa = 4.08 FVPPEE190 pKa = 4.0 NSFGAEE196 pKa = 3.74 DD197 pKa = 4.25 DD198 pKa = 4.24 EE199 pKa = 4.85 EE200 pKa = 5.19 EE201 pKa = 4.22 EE202 pKa = 4.48 TGASAQQAGSGGSGGPQVNGQEE224 pKa = 4.18 DD225 pKa = 4.83 LGTGGVPNVLGSPGTGGLPNANGLGIGPGEE255 pKa = 4.21 MVLSPGIGGQLGGLGVGGIGGGALAGMGGGVADD288 pKa = 4.25 AALLSGSQPNSAEE301 pKa = 4.15 VFTGADD307 pKa = 3.06 MAGLQEE313 pKa = 4.69 ALNSIHH319 pKa = 7.57 IDD321 pKa = 3.45 EE322 pKa = 5.84 LEE324 pKa = 4.31 GQPEE328 pKa = 4.25 LEE330 pKa = 4.52 DD331 pKa = 3.42
Molecular weight: 34.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.884
IPC_protein 3.859
Toseland 3.668
ProMoST 3.973
Dawson 3.834
Bjellqvist 4.012
Wikipedia 3.745
Rodwell 3.694
Grimsley 3.579
Solomon 3.834
Lehninger 3.783
Nozaki 3.948
DTASelect 4.139
Thurlkill 3.706
EMBOSS 3.757
Sillero 3.973
Patrickios 1.163
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.887
Protein with the highest isoelectric point:
>tr|W7I9Y4|W7I9Y4_9PEZI Uncharacterized protein OS=Drechslerella stenobrocha 248 OX=1043628 GN=DRE_00232 PE=4 SV=1
MM1 pKa = 7.94 PSQKK5 pKa = 10.46 SFRR8 pKa = 11.84 TKK10 pKa = 10.66 VKK12 pKa = 9.75 LAKK15 pKa = 9.97 AKK17 pKa = 9.94 KK18 pKa = 8.03 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.89 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.15 WRR45 pKa = 11.84 KK46 pKa = 7.38 TRR48 pKa = 11.84 IGII51 pKa = 4.0
Molecular weight: 6.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.391
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.384
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.106
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.993
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5595
0
5595
2891906
49
4954
516.9
57.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.77 ± 0.031
1.137 ± 0.012
5.824 ± 0.024
6.296 ± 0.033
3.551 ± 0.019
6.953 ± 0.031
2.262 ± 0.016
4.885 ± 0.021
5.075 ± 0.031
8.643 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.107 ± 0.012
3.69 ± 0.017
6.291 ± 0.042
3.942 ± 0.025
6.144 ± 0.029
8.198 ± 0.038
6.074 ± 0.028
6.144 ± 0.026
1.281 ± 0.012
2.734 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here