Serinicoccus chungangensis
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3100 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0W8IHA6|A0A0W8IHA6_9MICO Uncharacterized protein OS=Serinicoccus chungangensis OX=767452 GN=AVL62_06395 PE=4 SV=1
MM1 pKa = 7.1 QNVPRR6 pKa = 11.84 RR7 pKa = 11.84 AVAAASTAALAVTGLVALSPAVQAAGPGLVVNEE40 pKa = 4.28 VYY42 pKa = 10.83 GGGGNSGAVYY52 pKa = 8.72 THH54 pKa = 7.26 DD55 pKa = 3.54 FVEE58 pKa = 4.92 LVNSTDD64 pKa = 3.87 APVDD68 pKa = 3.73 LDD70 pKa = 4.18 GYY72 pKa = 10.06 AVSYY76 pKa = 10.23 YY77 pKa = 10.61 SSSGNLGNACPLSGTVEE94 pKa = 4.1 PGASYY99 pKa = 10.32 LIQQNPGSGGTTPLPDD115 pKa = 4.48 PDD117 pKa = 4.19 IEE119 pKa = 5.47 CGASMSGSNGIVEE132 pKa = 4.71 LSLDD136 pKa = 3.9 GEE138 pKa = 4.76 VVDD141 pKa = 4.71 LVGYY145 pKa = 10.28 GSATRR150 pKa = 11.84 FEE152 pKa = 4.82 GSGAAPGLSNTTSASRR168 pKa = 11.84 SDD170 pKa = 5.22 AVDD173 pKa = 3.38 TDD175 pKa = 4.61 DD176 pKa = 4.57 NAADD180 pKa = 4.07 FTRR183 pKa = 11.84 GEE185 pKa = 4.14 PTPTGSGGGTDD196 pKa = 4.74 PEE198 pKa = 4.74 PEE200 pKa = 4.68 PDD202 pKa = 4.21 PEE204 pKa = 4.77 PVDD207 pKa = 3.44 TTIAQIQGTGATSPLEE223 pKa = 4.09 GQPVNTSGVVTAAYY237 pKa = 7.02 PTGGFNGVYY246 pKa = 9.96 LQTAGSGGAAKK257 pKa = 10.6 APGDD261 pKa = 3.65 ASDD264 pKa = 4.91 GVFLYY269 pKa = 10.88 SSWAADD275 pKa = 3.29 NLEE278 pKa = 4.33 IGDD281 pKa = 4.26 CVEE284 pKa = 4.18 VEE286 pKa = 4.1 DD287 pKa = 5.57 GEE289 pKa = 4.61 VVEE292 pKa = 4.65 YY293 pKa = 11.24 NGLTEE298 pKa = 3.84 ISGGFVTVVDD308 pKa = 3.95 GCEE311 pKa = 3.78 AVVPTVLSSLPATDD325 pKa = 3.86 AEE327 pKa = 4.46 KK328 pKa = 10.56 EE329 pKa = 4.38 VYY331 pKa = 10.09 EE332 pKa = 4.37 GMLVQPEE339 pKa = 4.26 GTYY342 pKa = 10.34 TITNNYY348 pKa = 8.78 QLNQYY353 pKa = 8.5 GQLGLAVGDD362 pKa = 3.85 EE363 pKa = 4.48 PLYY366 pKa = 10.59 QATDD370 pKa = 3.41 QVLPGPEE377 pKa = 3.7 AEE379 pKa = 5.09 AYY381 pKa = 9.34 QAANQEE387 pKa = 4.37 KK388 pKa = 10.68 YY389 pKa = 8.76 ITLDD393 pKa = 4.52 DD394 pKa = 4.57 GSSWDD399 pKa = 3.6 YY400 pKa = 11.26 VRR402 pKa = 11.84 NSTAQDD408 pKa = 3.27 SPLPYY413 pKa = 10.42 LSAQEE418 pKa = 3.88 PHH420 pKa = 6.01 RR421 pKa = 11.84 TGSQVTFEE429 pKa = 4.27 QPVVLDD435 pKa = 5.5 YY436 pKa = 11.09 RR437 pKa = 11.84 FQWNYY442 pKa = 9.26 QPVGQVVGSDD452 pKa = 3.81 SPVDD456 pKa = 4.3 PISSEE461 pKa = 3.74 NDD463 pKa = 2.98 RR464 pKa = 11.84 EE465 pKa = 4.18 YY466 pKa = 11.18 AAPEE470 pKa = 3.77 VGGNIQIGAFNVLNYY485 pKa = 10.2 FSDD488 pKa = 3.97 LGQDD492 pKa = 3.83 EE493 pKa = 4.64 EE494 pKa = 5.12 DD495 pKa = 3.9 CDD497 pKa = 4.26 FFADD501 pKa = 4.04 RR502 pKa = 11.84 FGNPVATDD510 pKa = 3.8 FCEE513 pKa = 4.62 VRR515 pKa = 11.84 GAWSEE520 pKa = 3.95 QAFADD525 pKa = 3.89 QQAKK529 pKa = 10.07 LVTAINGTDD538 pKa = 3.57 AEE540 pKa = 4.52 VLALMEE546 pKa = 4.69 IEE548 pKa = 4.24 NSAALSYY555 pKa = 10.96 VDD557 pKa = 4.25 HH558 pKa = 7.11 PRR560 pKa = 11.84 DD561 pKa = 3.19 KK562 pKa = 11.32 ALADD566 pKa = 3.79 LVAALNAEE574 pKa = 4.25 AGEE577 pKa = 4.17 QRR579 pKa = 11.84 WAYY582 pKa = 10.15 APSPTVTPPNEE593 pKa = 4.14 DD594 pKa = 3.45 VIRR597 pKa = 11.84 TAFIYY602 pKa = 10.84 DD603 pKa = 3.65 PDD605 pKa = 3.82 QVQLLGPSMIQLDD618 pKa = 3.89 DD619 pKa = 3.69 AFANARR625 pKa = 11.84 YY626 pKa = 8.83 PLAQKK631 pKa = 10.36 FKK633 pKa = 10.33 ATGTGKK639 pKa = 9.81 PFVAVANHH647 pKa = 6.25 FKK649 pKa = 11.01 SKK651 pKa = 10.83 GSGEE655 pKa = 4.27 DD656 pKa = 3.57 DD657 pKa = 3.4 GTGQGNANPSRR668 pKa = 11.84 IAQAEE673 pKa = 4.06 ALTAWSEE680 pKa = 4.21 EE681 pKa = 3.98 MFEE684 pKa = 4.69 DD685 pKa = 4.41 EE686 pKa = 6.5 AIFLMGDD693 pKa = 3.48 FNAYY697 pKa = 9.82 SRR699 pKa = 11.84 EE700 pKa = 3.97 DD701 pKa = 3.45 PVRR704 pKa = 11.84 IIEE707 pKa = 4.39 DD708 pKa = 3.03 AGYY711 pKa = 8.96 TNLARR716 pKa = 11.84 ADD718 pKa = 4.22 EE719 pKa = 4.91 PDD721 pKa = 3.07 SMTYY725 pKa = 10.04 QFSGRR730 pKa = 11.84 LGSLDD735 pKa = 3.49 HH736 pKa = 6.92 VFANEE741 pKa = 3.45 KK742 pKa = 10.04 AQRR745 pKa = 11.84 LVTGAGVWDD754 pKa = 3.79 INADD758 pKa = 3.44 EE759 pKa = 5.23 SIAFQYY765 pKa = 10.49 SRR767 pKa = 11.84 RR768 pKa = 11.84 NYY770 pKa = 10.23 NVVDD774 pKa = 4.23 FHH776 pKa = 9.45 SDD778 pKa = 3.36 DD779 pKa = 3.58 QFASSDD785 pKa = 3.68 HH786 pKa = 7.02 DD787 pKa = 3.92 PVLVGLDD794 pKa = 3.06 TGNRR798 pKa = 11.84 GKK800 pKa = 10.93 GKK802 pKa = 10.06 NN803 pKa = 3.34
Molecular weight: 84.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.834
IPC_protein 3.859
Toseland 3.643
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.681
Grimsley 3.541
Solomon 3.834
Lehninger 3.795
Nozaki 3.935
DTASelect 4.164
Thurlkill 3.681
EMBOSS 3.757
Sillero 3.973
Patrickios 1.545
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.858
Protein with the highest isoelectric point:
>tr|A0A0W8I4D2|A0A0W8I4D2_9MICO Uncharacterized protein OS=Serinicoccus chungangensis OX=767452 GN=AVL62_14825 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.97 KK16 pKa = 9.33 HH17 pKa = 4.25 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 SILNARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.28 GRR40 pKa = 11.84 SSISAA45 pKa = 3.4
Molecular weight: 5.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3100
0
3100
1026549
37
1907
331.1
35.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.987 ± 0.061
0.655 ± 0.012
6.416 ± 0.038
6.192 ± 0.043
2.481 ± 0.027
9.712 ± 0.041
2.288 ± 0.026
2.871 ± 0.031
1.32 ± 0.028
10.63 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.84 ± 0.017
1.355 ± 0.02
5.88 ± 0.032
3.104 ± 0.023
8.053 ± 0.059
4.965 ± 0.031
6.069 ± 0.033
9.744 ± 0.047
1.627 ± 0.019
1.811 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here