Mycolicibacterium diernhoferi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia;

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5529 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q4HAQ7|A0A1Q4HAQ7_9MYCO GTPase HflX OS=Mycolicibacterium diernhoferi OX=1801 GN=hflX PE=3 SV=1
MM1 pKa = 7.26VLTGGLTGCSSAAAAAFQVGDD22 pKa = 3.87CLKK25 pKa = 11.01VVGTVDD31 pKa = 3.89RR32 pKa = 11.84PDD34 pKa = 3.34AVKK37 pKa = 10.12AACGSPDD44 pKa = 3.18STFKK48 pKa = 11.1VVATVADD55 pKa = 4.01SGEE58 pKa = 4.35CPSDD62 pKa = 3.2VDD64 pKa = 4.82SYY66 pKa = 11.52YY67 pKa = 10.15ATHH70 pKa = 6.57NAFSDD75 pKa = 3.62TSTTVCMDD83 pKa = 3.39VDD85 pKa = 3.87WVLGQCMSVDD95 pKa = 3.84PDD97 pKa = 3.35NGRR100 pKa = 11.84DD101 pKa = 3.69PVRR104 pKa = 11.84VDD106 pKa = 4.25CRR108 pKa = 11.84DD109 pKa = 3.36DD110 pKa = 3.72AQPNRR115 pKa = 11.84QRR117 pKa = 11.84ATQILQGITDD127 pKa = 4.18ADD129 pKa = 3.66QCRR132 pKa = 11.84SGMGYY137 pKa = 9.71PYY139 pKa = 10.15DD140 pKa = 3.57EE141 pKa = 5.3RR142 pKa = 11.84GFTVCVDD149 pKa = 3.48GVDD152 pKa = 3.24

Molecular weight: 15.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q4HMH7|A0A1Q4HMH7_9MYCO Uncharacterized protein OS=Mycolicibacterium diernhoferi OX=1801 GN=BRW64_03245 PE=3 SV=1
MM1 pKa = 7.18AHH3 pKa = 6.45SKK5 pKa = 10.34KK6 pKa = 10.34SHH8 pKa = 4.51TRR10 pKa = 11.84RR11 pKa = 11.84TGHH14 pKa = 5.64VPGPAGAHH22 pKa = 4.86VPNRR26 pKa = 11.84PLHH29 pKa = 5.46SVEE32 pKa = 4.17VLPAPGHH39 pKa = 6.36DD40 pKa = 2.94ASVWGRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84VLLLNSTYY56 pKa = 10.82EE57 pKa = 4.11PLTALPMRR65 pKa = 11.84RR66 pKa = 11.84AVIMLMCGKK75 pKa = 10.39ADD77 pKa = 3.62VVHH80 pKa = 7.69DD81 pKa = 4.67DD82 pKa = 3.42PSGPVIHH89 pKa = 6.63SATRR93 pKa = 11.84SIVVPSVIRR102 pKa = 11.84LRR104 pKa = 11.84TFVRR108 pKa = 11.84VPYY111 pKa = 9.95RR112 pKa = 11.84ARR114 pKa = 11.84IPMTRR119 pKa = 11.84AALMHH124 pKa = 6.99RR125 pKa = 11.84DD126 pKa = 3.56RR127 pKa = 11.84FRR129 pKa = 11.84CAYY132 pKa = 9.59CGQKK136 pKa = 10.53ADD138 pKa = 4.07TVDD141 pKa = 3.41HH142 pKa = 5.53VVPRR146 pKa = 11.84SRR148 pKa = 11.84GGEE151 pKa = 3.98HH152 pKa = 5.99SWEE155 pKa = 4.09NCVAACSACNHH166 pKa = 6.13RR167 pKa = 11.84KK168 pKa = 9.61ADD170 pKa = 4.0RR171 pKa = 11.84LLAEE175 pKa = 5.2LGWSLRR181 pKa = 11.84SVPMPPKK188 pKa = 8.32GQHH191 pKa = 4.48WRR193 pKa = 11.84LLSSVKK199 pKa = 10.64DD200 pKa = 4.05LDD202 pKa = 4.38PSWVRR207 pKa = 11.84YY208 pKa = 9.53LGEE211 pKa = 4.08GAAA214 pKa = 4.63

Molecular weight: 23.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5529

0

5529

1805390

31

3663

326.5

34.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.109 ± 0.043

0.78 ± 0.009

6.305 ± 0.022

5.334 ± 0.035

3.097 ± 0.017

8.992 ± 0.031

2.144 ± 0.015

4.372 ± 0.02

2.152 ± 0.021

9.87 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.026 ± 0.015

2.229 ± 0.018

5.815 ± 0.032

2.949 ± 0.016

7.01 ± 0.03

5.318 ± 0.023

6.169 ± 0.028

8.713 ± 0.03

1.463 ± 0.014

2.153 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski