Wuhan flea virus

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; unclassified ssRNA positive-strand viruses

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0QKU2|A0A0P0QKU2_9VIRU Putative capsid protein OS=Wuhan flea virus OX=1746071 GN=VP2 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 10.62SNLCFIILTLLTTNILGKK20 pKa = 10.45DD21 pKa = 3.27RR22 pKa = 11.84DD23 pKa = 3.95CGKK26 pKa = 10.46FSEE29 pKa = 4.16NWRR32 pKa = 11.84ISACASGLKK41 pKa = 9.19QANLISDD48 pKa = 4.19ATYY51 pKa = 11.09VEE53 pKa = 4.49YY54 pKa = 10.81LLKK57 pKa = 10.8AIIATNGPNDD67 pKa = 3.95FYY69 pKa = 11.45GAGMTTGLDD78 pKa = 3.41TQLVIGKK85 pKa = 8.43MSTPVLSSGLSFNYY99 pKa = 9.68HH100 pKa = 6.63PSSMYY105 pKa = 10.09YY106 pKa = 9.74EE107 pKa = 4.01RR108 pKa = 11.84HH109 pKa = 5.62LVDD112 pKa = 5.09LGVKK116 pKa = 10.1FDD118 pKa = 4.06RR119 pKa = 11.84PVALFTIDD127 pKa = 3.22TMGSFPEE134 pKa = 4.15WTDD137 pKa = 3.09TMFMKK142 pKa = 9.87FVPTGSEE149 pKa = 3.19TRR151 pKa = 11.84YY152 pKa = 9.57AAFRR156 pKa = 11.84AGDD159 pKa = 3.85GSPVNIHH166 pKa = 5.49GQEE169 pKa = 3.74HH170 pKa = 6.48CADD173 pKa = 3.4FTKK176 pKa = 10.28WVDD179 pKa = 3.34SKK181 pKa = 11.55LPEE184 pKa = 4.53KK185 pKa = 10.66KK186 pKa = 9.89QGLAIHH192 pKa = 6.56MSQTLWCPVLKK203 pKa = 10.59DD204 pKa = 3.89GIMEE208 pKa = 4.1IVVAKK213 pKa = 10.58LNEE216 pKa = 3.96EE217 pKa = 3.96HH218 pKa = 7.28RR219 pKa = 11.84IAPNGEE225 pKa = 4.08SEE227 pKa = 4.33KK228 pKa = 10.89VLIVEE233 pKa = 4.24WAIWGSGAYY242 pKa = 10.15GDD244 pKa = 4.03VPEE247 pKa = 5.27EE248 pKa = 4.09YY249 pKa = 10.69DD250 pKa = 3.65SVFYY254 pKa = 11.12

Molecular weight: 28.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0QKM5|A0A0P0QKM5_9VIRU Uncharacterized protein OS=Wuhan flea virus OX=1746071 GN=VP3 PE=4 SV=1
MM1 pKa = 7.99RR2 pKa = 11.84ICVCLILLIVSLTHH16 pKa = 7.36ASTSQQQARR25 pKa = 11.84SQEE28 pKa = 4.17LKK30 pKa = 10.87SEE32 pKa = 4.27LEE34 pKa = 4.32KK35 pKa = 10.73AWKK38 pKa = 10.13LDD40 pKa = 3.71FEE42 pKa = 4.8GLSLDD47 pKa = 3.47PTAWSSTLAGKK58 pKa = 10.01LSKK61 pKa = 10.73VAVGVGYY68 pKa = 11.34AMTLPMSLAHH78 pKa = 5.97VVGFGLFLFGLVRR91 pKa = 11.84LTWGIVGTSCLALYY105 pKa = 8.93GWKK108 pKa = 9.0YY109 pKa = 10.07HH110 pKa = 6.06KK111 pKa = 10.36RR112 pKa = 11.84SFLVAGVGLTYY123 pKa = 11.27ALAFGLSTLYY133 pKa = 10.99

Molecular weight: 14.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2939

133

911

489.8

55.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.397 ± 0.619

1.395 ± 0.229

4.389 ± 0.416

6.635 ± 0.363

3.505 ± 0.253

8.098 ± 0.319

2.28 ± 0.213

6.703 ± 0.588

5.444 ± 0.735

9.969 ± 0.693

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.062 ± 0.216

3.607 ± 0.231

3.981 ± 0.332

2.79 ± 0.168

6.261 ± 0.736

5.648 ± 0.441

6.805 ± 0.476

6.975 ± 0.434

2.382 ± 0.389

3.675 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski