Persimmon virus B

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A8JEL2|A0A0A8JEL2_9CLOS RNA-dependent RNA polymerase (Fragment) OS=Persimmon virus B OX=1493829 GN=RdRp PE=4 SV=1
MM1 pKa = 7.86DD2 pKa = 4.4SKK4 pKa = 11.64VIVTLNLNGSLSAIHH19 pKa = 6.74NSDD22 pKa = 3.48TNVNEE27 pKa = 3.7QSIIEE32 pKa = 4.19SLFIKK37 pKa = 9.99NWFRR41 pKa = 11.84ITDD44 pKa = 3.72DD45 pKa = 3.9LAVEE49 pKa = 4.18EE50 pKa = 4.47TVRR53 pKa = 11.84LLIEE57 pKa = 4.39SEE59 pKa = 4.08SGKK62 pKa = 10.68YY63 pKa = 9.73KK64 pKa = 10.27YY65 pKa = 10.38LSNKK69 pKa = 9.29GCVSEE74 pKa = 5.31LALDD78 pKa = 4.52LNVLLNDD85 pKa = 4.33FGISTGQKK93 pKa = 6.64TVRR96 pKa = 11.84TLVFGSDD103 pKa = 3.19QSNLLKK109 pKa = 11.07SMLISSHH116 pKa = 6.5YY117 pKa = 8.44YY118 pKa = 9.43LSVYY122 pKa = 10.22FLDD125 pKa = 3.74YY126 pKa = 11.29AVFSVVKK133 pKa = 9.76PWEE136 pKa = 4.3CVTNDD141 pKa = 3.06TYY143 pKa = 11.66HH144 pKa = 6.29FVVNNKK150 pKa = 9.1IDD152 pKa = 4.6LEE154 pKa = 4.49DD155 pKa = 4.12DD156 pKa = 3.64CAIIPGMRR164 pKa = 11.84WMCC167 pKa = 3.93

Molecular weight: 18.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A8JCX5|A0A0A8JCX5_9CLOS 19 kDa protein OS=Persimmon virus B OX=1493829 GN=p19 PE=4 SV=1
MM1 pKa = 7.47RR2 pKa = 11.84RR3 pKa = 11.84AGSMTMPVQTRR14 pKa = 11.84YY15 pKa = 7.48WQPGHH20 pKa = 7.18LFRR23 pKa = 11.84IFFGRR28 pKa = 11.84QDD30 pKa = 2.94IEE32 pKa = 4.81KK33 pKa = 9.83YY34 pKa = 9.78VGEE37 pKa = 5.03AISTYY42 pKa = 8.6YY43 pKa = 10.15NRR45 pKa = 11.84DD46 pKa = 2.99YY47 pKa = 8.32TTQVQYY53 pKa = 11.68VFGDD57 pKa = 3.47SFRR60 pKa = 11.84IITLSPYY67 pKa = 8.54VTSTNSISTAYY78 pKa = 10.54NEE80 pKa = 3.88YY81 pKa = 10.46NLLYY85 pKa = 10.16FYY87 pKa = 10.18VIRR90 pKa = 11.84GGYY93 pKa = 7.28SSKK96 pKa = 10.64VVGYY100 pKa = 9.47QPYY103 pKa = 9.87ILLRR107 pKa = 11.84GILEE111 pKa = 4.1RR112 pKa = 11.84DD113 pKa = 2.92ISTVIMTPLINLSKK127 pKa = 10.94DD128 pKa = 3.64RR129 pKa = 11.84LWANMKK135 pKa = 10.09FKK137 pKa = 11.02SSDD140 pKa = 3.25PRR142 pKa = 11.84FLTLKK147 pKa = 10.85AEE149 pKa = 4.21DD150 pKa = 3.39RR151 pKa = 11.84QFYY154 pKa = 11.04VNLSLVTGEE163 pKa = 4.29FTTSVAKK170 pKa = 9.78TRR172 pKa = 11.84EE173 pKa = 4.27AKK175 pKa = 10.42DD176 pKa = 3.62VFPDD180 pKa = 3.44IPVSEE185 pKa = 4.61SVSSGLPLKK194 pKa = 10.84YY195 pKa = 10.01ILPKK199 pKa = 10.75LFFHH203 pKa = 7.79GIRR206 pKa = 11.84VLNGNVATKK215 pKa = 10.49LYY217 pKa = 9.59VQYY220 pKa = 11.27NHH222 pKa = 5.98VLAVLSNILSSYY234 pKa = 10.15NHH236 pKa = 6.73LFMLNSSFTHH246 pKa = 5.07YY247 pKa = 10.9AITFFGWMAMFFDD260 pKa = 5.49NYY262 pKa = 10.93SQLNYY267 pKa = 10.51EE268 pKa = 4.55SGEE271 pKa = 3.82KK272 pKa = 9.25WEE274 pKa = 4.13EE275 pKa = 3.67VMRR278 pKa = 11.84FCTHH282 pKa = 7.38PLTSFFIKK290 pKa = 10.49FDD292 pKa = 4.17DD293 pKa = 4.52SDD295 pKa = 3.7LQRR298 pKa = 11.84IRR300 pKa = 11.84WITPDD305 pKa = 3.13RR306 pKa = 11.84FEE308 pKa = 5.94KK309 pKa = 10.13VLQYY313 pKa = 11.4VNYY316 pKa = 9.93VSYY319 pKa = 11.23GGLALSDD326 pKa = 3.91YY327 pKa = 11.12QEE329 pKa = 5.51LIWTPKK335 pKa = 10.46VYY337 pKa = 10.5DD338 pKa = 4.16KK339 pKa = 11.57EE340 pKa = 4.7SFCQLSAIFVSLVSTNKK357 pKa = 9.63RR358 pKa = 11.84VEE360 pKa = 4.53PILILGAIIAYY371 pKa = 9.16YY372 pKa = 9.82SIHH375 pKa = 5.09GTCRR379 pKa = 11.84YY380 pKa = 9.7RR381 pKa = 11.84SEE383 pKa = 3.58KK384 pKa = 9.59RR385 pKa = 11.84PRR387 pKa = 11.84YY388 pKa = 9.42FKK390 pKa = 10.43FYY392 pKa = 10.52SHH394 pKa = 6.88GEE396 pKa = 4.02EE397 pKa = 4.01IVCDD401 pKa = 4.88FKK403 pKa = 11.38FLEE406 pKa = 4.4IKK408 pKa = 10.32FDD410 pKa = 3.43MMQNFVKK417 pKa = 10.12AYY419 pKa = 7.9SVRR422 pKa = 11.84RR423 pKa = 11.84VYY425 pKa = 10.72LGSAGDD431 pKa = 3.66LCVDD435 pKa = 4.27FLRR438 pKa = 11.84SIGLEE443 pKa = 3.76LPLRR447 pKa = 11.84WKK449 pKa = 10.21RR450 pKa = 11.84VNWLPYY456 pKa = 9.67KK457 pKa = 10.09DD458 pKa = 3.72VRR460 pKa = 11.84YY461 pKa = 10.2VDD463 pKa = 3.55YY464 pKa = 10.75HH465 pKa = 6.67KK466 pKa = 10.47HH467 pKa = 3.83VTNKK471 pKa = 9.98NMLKK475 pKa = 10.24KK476 pKa = 10.54GIRR479 pKa = 11.84ASIGAYY485 pKa = 9.99GNVVSTNAFEE495 pKa = 4.0ARR497 pKa = 11.84KK498 pKa = 8.88WRR500 pKa = 11.84DD501 pKa = 2.7LWW503 pKa = 5.05

Molecular weight: 59.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

1

12

5961

69

3149

496.8

56.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.207 ± 0.523

2.399 ± 0.341

5.838 ± 0.343

5.955 ± 0.481

4.546 ± 0.455

5.871 ± 0.498

1.627 ± 0.182

5.788 ± 0.659

6.123 ± 0.243

8.757 ± 0.468

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.298 ± 0.216

4.462 ± 0.706

3.942 ± 0.693

2.214 ± 0.136

6.123 ± 0.479

8.489 ± 0.313

4.815 ± 0.431

8.824 ± 0.433

1.174 ± 0.185

4.546 ± 0.698
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski