Dendrobium catenatum
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 29029 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I0W6M1|A0A2I0W6M1_9ASPA Polyol transporter 5 OS=Dendrobium catenatum OX=906689 GN=PLT5 PE=3 SV=1
MM1 pKa = 8.04 VVMSVLVVVGPDD13 pKa = 4.18 GIVSDD18 pKa = 4.79 DD19 pKa = 3.99 SVVHH23 pKa = 6.6 DD24 pKa = 4.71 LAVNLDD30 pKa = 4.07 DD31 pKa = 6.53 DD32 pKa = 5.33 LPAPSNSPISHH43 pKa = 5.96 IVVSPILPYY52 pKa = 9.9 MGGISPILDD61 pKa = 3.54 SEE63 pKa = 4.57 LVVYY67 pKa = 10.68 SGMDD71 pKa = 3.29 GVVSPNVAPSIEE83 pKa = 4.25 VVSDD87 pKa = 3.24 INCCVNLTDD96 pKa = 4.08 SPSYY100 pKa = 11.41 ADD102 pKa = 4.82 GADD105 pKa = 3.57 LDD107 pKa = 4.34 VGKK110 pKa = 10.16 SAYY113 pKa = 9.8 LVNSGKK119 pKa = 9.92 EE120 pKa = 3.48 ALAIIRR126 pKa = 11.84 EE127 pKa = 4.24 ASVKK131 pKa = 9.99 VPQVDD136 pKa = 3.94 VPILL140 pKa = 3.55
Molecular weight: 14.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.643
IPC_protein 3.643
Toseland 3.414
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.643
Rodwell 3.465
Grimsley 3.325
Solomon 3.643
Lehninger 3.605
Nozaki 3.783
DTASelect 4.075
Thurlkill 3.49
EMBOSS 3.656
Sillero 3.77
Patrickios 0.846
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.713
Protein with the highest isoelectric point:
>tr|A0A2I0X704|A0A2I0X704_9ASPA Disease resistance protein RGA4 OS=Dendrobium catenatum OX=906689 GN=RGA4 PE=3 SV=1
MM1 pKa = 7.56 LLKK4 pKa = 9.78 TVAKK8 pKa = 9.0 MILRR12 pKa = 11.84 ILTLRR17 pKa = 11.84 SLRR20 pKa = 11.84 RR21 pKa = 11.84 PNWSSLTFNVKK32 pKa = 9.66 KK33 pKa = 10.56 RR34 pKa = 11.84 LIRR37 pKa = 11.84 SRR39 pKa = 11.84 RR40 pKa = 11.84 LPLSSWRR47 pKa = 11.84 KK48 pKa = 7.05 GTIILVIFMPWIARR62 pKa = 11.84 SVLLMVFIKK71 pKa = 9.78 LTVVMVLLLRR81 pKa = 11.84 MVMRR85 pKa = 11.84 LLPLLLIFFNITLIKK100 pKa = 10.33 ISVLPPLMIILLSSVKK116 pKa = 10.56
Molecular weight: 13.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.524
IPC2_protein 11.301
IPC_protein 12.881
Toseland 13.056
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.778
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.056
DTASelect 13.042
Thurlkill 13.056
EMBOSS 13.554
Sillero 13.056
Patrickios 12.501
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.195
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
29029
0
29029
9774483
49
5084
336.7
37.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.944 ± 0.013
1.908 ± 0.008
5.133 ± 0.011
6.262 ± 0.015
4.434 ± 0.01
6.351 ± 0.014
2.461 ± 0.007
5.646 ± 0.011
5.837 ± 0.013
10.015 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.416 ± 0.006
4.342 ± 0.013
4.984 ± 0.016
3.506 ± 0.01
5.653 ± 0.012
9.133 ± 0.019
4.691 ± 0.012
6.224 ± 0.012
1.331 ± 0.006
2.727 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here