Salmonella phage vB_SenS_PHB07
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2R3U9I0|A0A2R3U9I0_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenS_PHB07 OX=2136179 PE=4 SV=1
MM1 pKa = 7.94 KK2 pKa = 10.17 YY3 pKa = 10.04 QIYY6 pKa = 10.22 SAIDD10 pKa = 4.4 AIDD13 pKa = 4.28 DD14 pKa = 4.03 AQSLFMFDD22 pKa = 4.17 EE23 pKa = 4.4 NDD25 pKa = 3.42 NYY27 pKa = 11.13 TVVCDD32 pKa = 4.75 CYY34 pKa = 11.03 TDD36 pKa = 4.17 ADD38 pKa = 4.19 YY39 pKa = 11.65 LEE41 pKa = 4.02 QLARR45 pKa = 11.84 NGEE48 pKa = 4.14 GCNWSDD54 pKa = 4.45 NKK56 pKa = 11.0 NEE58 pKa = 4.14 TPRR61 pKa = 11.84 RR62 pKa = 11.84 QDD64 pKa = 2.95 MINPVLVKK72 pKa = 10.43 EE73 pKa = 4.48 FEE75 pKa = 4.25 VV76 pKa = 3.55
Molecular weight: 8.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.897
IPC_protein 3.846
Toseland 3.63
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.783
Rodwell 3.668
Grimsley 3.541
Solomon 3.821
Lehninger 3.783
Nozaki 3.961
DTASelect 4.19
Thurlkill 3.694
EMBOSS 3.795
Sillero 3.961
Patrickios 0.846
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.861
Protein with the highest isoelectric point:
>tr|A0A2R3U9F9|A0A2R3U9F9_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenS_PHB07 OX=2136179 PE=4 SV=1
MM1 pKa = 7.37 FCPIRR6 pKa = 11.84 FNGVDD11 pKa = 3.64 LLKK14 pKa = 10.3 EE15 pKa = 4.09 SRR17 pKa = 11.84 MCTTAIVNRR26 pKa = 11.84 LYY28 pKa = 10.91 LRR30 pKa = 11.84 SGGNIVKK37 pKa = 10.48 LEE39 pKa = 3.91 YY40 pKa = 10.08 RR41 pKa = 11.84 SGRR44 pKa = 11.84 IIRR47 pKa = 11.84 TDD49 pKa = 2.97 TGRR52 pKa = 11.84 WIEE55 pKa = 4.14 TNEE58 pKa = 4.1 LLWHH62 pKa = 5.66 NAKK65 pKa = 10.09 GRR67 pKa = 11.84 LLFKK71 pKa = 10.22 TFEE74 pKa = 5.07 DD75 pKa = 3.57 IRR77 pKa = 5.28
Molecular weight: 9.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.271
IPC2_protein 9.268
IPC_protein 9.589
Toseland 10.16
ProMoST 10.101
Dawson 10.321
Bjellqvist 10.014
Wikipedia 10.482
Rodwell 10.57
Grimsley 10.379
Solomon 10.394
Lehninger 10.365
Nozaki 10.204
DTASelect 9.999
Thurlkill 10.189
EMBOSS 10.555
Sillero 10.248
Patrickios 10.394
IPC_peptide 10.394
IPC2_peptide 9.063
IPC2.peptide.svr19 8.273
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
87
0
87
15683
41
1259
180.3
20.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.238 ± 0.455
1.428 ± 0.194
6.16 ± 0.216
7.046 ± 0.241
3.934 ± 0.198
7.32 ± 0.27
1.913 ± 0.22
6.421 ± 0.185
7.244 ± 0.388
6.944 ± 0.226
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.156 ± 0.185
4.942 ± 0.283
3.303 ± 0.201
4.1 ± 0.302
4.935 ± 0.221
5.605 ± 0.228
5.42 ± 0.208
6.893 ± 0.307
1.428 ± 0.094
3.571 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here