Amino acid dipepetide frequency for Novosphingobium umbonatum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.162AlaAla: 18.162 ± 0.174	1.187AlaCys: 1.187 ± 0.033	6.905AlaAsp: 6.905 ± 0.089	7.221AlaGlu: 7.221 ± 0.093	4.132AlaPhe: 4.132 ± 0.066	11.595AlaGly: 11.595 ± 0.113	2.638AlaHis: 2.638 ± 0.053	6.159AlaIle: 6.159 ± 0.073	5.045AlaLys: 5.045 ± 0.086	14.94AlaLeu: 14.94 ± 0.152	4.37AlaMet: 4.37 ± 0.071	3.484AlaAsn: 3.484 ± 0.054	6.743AlaPro: 6.743 ± 0.114	6.321AlaGln: 6.321 ± 0.09	8.808AlaArg: 8.808 ± 0.102	7.331AlaSer: 7.331 ± 0.083	6.232AlaThr: 6.232 ± 0.081	8.206AlaVal: 8.206 ± 0.085	1.712AlaTrp: 1.712 ± 0.044	3.024AlaTyr: 3.024 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	1.073CysAla: 1.073 ± 0.028	0.095CysCys: 0.095 ± 0.009	0.502CysAsp: 0.502 ± 0.021	0.399CysGlu: 0.399 ± 0.02	0.304CysPhe: 0.304 ± 0.018	0.913CysGly: 0.913 ± 0.027	0.252CysHis: 0.252 ± 0.016	0.363CysIle: 0.363 ± 0.018	0.201CysLys: 0.201 ± 0.012	0.769CysLeu: 0.769 ± 0.026	0.169CysMet: 0.169 ± 0.012	0.239CysAsn: 0.239 ± 0.015	0.5CysPro: 0.5 ± 0.02	0.261CysGln: 0.261 ± 0.014	0.524CysArg: 0.524 ± 0.019	0.51CysSer: 0.51 ± 0.02	0.404CysThr: 0.404 ± 0.02	0.588CysVal: 0.588 ± 0.022	0.133CysTrp: 0.133 ± 0.011	0.193CysTyr: 0.193 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	6.866AspAla: 6.866 ± 0.078	0.487AspCys: 0.487 ± 0.02	2.64AspAsp: 2.64 ± 0.057	2.934AspGlu: 2.934 ± 0.052	2.11AspPhe: 2.11 ± 0.039	5.316AspGly: 5.316 ± 0.07	1.294AspHis: 1.294 ± 0.034	2.75AspIle: 2.75 ± 0.043	1.952AspLys: 1.952 ± 0.038	5.646AspLeu: 5.646 ± 0.074	1.408AspMet: 1.408 ± 0.036	1.414AspAsn: 1.414 ± 0.033	3.212AspPro: 3.212 ± 0.05	1.812AspGln: 1.812 ± 0.039	3.693AspArg: 3.693 ± 0.061	2.306AspSer: 2.306 ± 0.053	2.323AspThr: 2.323 ± 0.046	3.767AspVal: 3.767 ± 0.056	1.145AspTrp: 1.145 ± 0.031	1.728AspTyr: 1.728 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	7.874GluAla: 7.874 ± 0.106	0.327GluCys: 0.327 ± 0.016	2.683GluAsp: 2.683 ± 0.054	2.897GluGlu: 2.897 ± 0.064	1.507GluPhe: 1.507 ± 0.035	4.403GluGly: 4.403 ± 0.07	1.12GluHis: 1.12 ± 0.031	2.557GluIle: 2.557 ± 0.049	2.111GluLys: 2.111 ± 0.034	4.633GluLeu: 4.633 ± 0.058	1.414GluMet: 1.414 ± 0.032	1.345GluAsn: 1.345 ± 0.037	2.163GluPro: 2.163 ± 0.043	2.265GluGln: 2.265 ± 0.051	3.993GluArg: 3.993 ± 0.064	2.157GluSer: 2.157 ± 0.046	2.64GluThr: 2.64 ± 0.052	3.621GluVal: 3.621 ± 0.067	0.781GluTrp: 0.781 ± 0.024	0.919GluTyr: 0.919 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	4.784PheAla: 4.784 ± 0.067	0.342PheCys: 0.342 ± 0.016	2.468PheAsp: 2.468 ± 0.046	1.733PheGlu: 1.733 ± 0.036	1.238PhePhe: 1.238 ± 0.035	3.545PheGly: 3.545 ± 0.056	0.756PheHis: 0.756 ± 0.02	1.495PheIle: 1.495 ± 0.038	0.952PheLys: 0.952 ± 0.029	2.975PheLeu: 2.975 ± 0.061	0.773PheMet: 0.773 ± 0.026	1.181PheAsn: 1.181 ± 0.039	1.53PhePro: 1.53 ± 0.038	0.929PheGln: 0.929 ± 0.027	1.986PheArg: 1.986 ± 0.04	2.085PheSer: 2.085 ± 0.047	2.085PheThr: 2.085 ± 0.044	2.435PheVal: 2.435 ± 0.041	0.521PheTrp: 0.521 ± 0.024	0.998PheTyr: 0.998 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	10.153GlyAla: 10.153 ± 0.093	0.866GlyCys: 0.866 ± 0.023	4.57GlyAsp: 4.57 ± 0.07	4.695GlyGlu: 4.695 ± 0.058	3.66GlyPhe: 3.66 ± 0.062	7.947GlyGly: 7.947 ± 0.115	2.006GlyHis: 2.006 ± 0.049	4.126GlyIle: 4.126 ± 0.06	3.643GlyLys: 3.643 ± 0.066	9.415GlyLeu: 9.415 ± 0.109	2.509GlyMet: 2.509 ± 0.043	2.446GlyAsn: 2.446 ± 0.06	3.447GlyPro: 3.447 ± 0.059	3.672GlyGln: 3.672 ± 0.057	5.726GlyArg: 5.726 ± 0.076	4.697GlySer: 4.697 ± 0.083	4.54GlyThr: 4.54 ± 0.077	6.518GlyVal: 6.518 ± 0.077	1.692GlyTrp: 1.692 ± 0.042	2.368GlyTyr: 2.368 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.575HisAla: 2.575 ± 0.053	0.23HisCys: 0.23 ± 0.013	1.197HisAsp: 1.197 ± 0.032	0.967HisGlu: 0.967 ± 0.031	0.94HisPhe: 0.94 ± 0.029	2.145HisGly: 2.145 ± 0.043	0.612HisHis: 0.612 ± 0.022	1.031HisIle: 1.031 ± 0.026	0.53HisLys: 0.53 ± 0.023	2.065HisLeu: 2.065 ± 0.042	0.496HisMet: 0.496 ± 0.019	0.54HisAsn: 0.54 ± 0.02	1.318HisPro: 1.318 ± 0.033	0.652HisGln: 0.652 ± 0.022	1.416HisArg: 1.416 ± 0.037	0.972HisSer: 0.972 ± 0.029	0.776HisThr: 0.776 ± 0.026	1.483HisVal: 1.483 ± 0.036	0.419HisTrp: 0.419 ± 0.019	0.703HisTyr: 0.703 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.988IleAla: 6.988 ± 0.08	0.489IleCys: 0.489 ± 0.018	3.224IleAsp: 3.224 ± 0.053	2.947IleGlu: 2.947 ± 0.051	1.438IlePhe: 1.438 ± 0.038	4.492IleGly: 4.492 ± 0.061	0.87IleHis: 0.87 ± 0.029	2.112IleIle: 2.112 ± 0.048	1.433IleLys: 1.433 ± 0.038	3.641IleLeu: 3.641 ± 0.066	0.948IleMet: 0.948 ± 0.032	1.529IleAsn: 1.529 ± 0.039	2.083IlePro: 2.083 ± 0.044	1.147IleGln: 1.147 ± 0.034	2.893IleArg: 2.893 ± 0.049	2.733IleSer: 2.733 ± 0.052	3.019IleThr: 3.019 ± 0.055	3.258IleVal: 3.258 ± 0.052	0.627IleTrp: 0.627 ± 0.02	1.063IleTyr: 1.063 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	5.032LysAla: 5.032 ± 0.083	0.139LysCys: 0.139 ± 0.012	1.716LysAsp: 1.716 ± 0.043	1.388LysGlu: 1.388 ± 0.039	0.972LysPhe: 0.972 ± 0.029	3.321LysGly: 3.321 ± 0.063	0.561LysHis: 0.561 ± 0.024	1.572LysIle: 1.572 ± 0.039	1.18LysLys: 1.18 ± 0.038	3.611LysLeu: 3.611 ± 0.061	0.823LysMet: 0.823 ± 0.026	0.828LysAsn: 0.828 ± 0.027	2.148LysPro: 2.148 ± 0.044	1.126LysGln: 1.126 ± 0.033	2.176LysArg: 2.176 ± 0.045	1.766LysSer: 1.766 ± 0.039	1.741LysThr: 1.741 ± 0.044	2.558LysVal: 2.558 ± 0.055	0.395LysTrp: 0.395 ± 0.019	0.577LysTyr: 0.577 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	15.259LeuAla: 15.259 ± 0.138	0.907LeuCys: 0.907 ± 0.03	5.734LeuAsp: 5.734 ± 0.089	4.766LeuGlu: 4.766 ± 0.061	3.323LeuPhe: 3.323 ± 0.061	9.092LeuGly: 9.092 ± 0.094	2.033LeuHis: 2.033 ± 0.041	4.296LeuIle: 4.296 ± 0.07	3.069LeuLys: 3.069 ± 0.051	9.343LeuLeu: 9.343 ± 0.111	2.311LeuMet: 2.311 ± 0.041	2.636LeuAsn: 2.636 ± 0.053	5.908LeuPro: 5.908 ± 0.067	2.856LeuGln: 2.856 ± 0.049	7.089LeuArg: 7.089 ± 0.092	6.362LeuSer: 6.362 ± 0.065	5.598LeuThr: 5.598 ± 0.066	7.373LeuVal: 7.373 ± 0.095	1.293LeuTrp: 1.293 ± 0.036	2.062LeuTyr: 2.062 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	3.997MetAla: 3.997 ± 0.062	0.152MetCys: 0.152 ± 0.012	1.254MetAsp: 1.254 ± 0.038	1.154MetGlu: 1.154 ± 0.028	0.618MetPhe: 0.618 ± 0.024	2.347MetGly: 2.347 ± 0.05	0.442MetHis: 0.442 ± 0.019	1.145MetIle: 1.145 ± 0.034	0.917MetLys: 0.917 ± 0.026	2.723MetLeu: 2.723 ± 0.051	0.733MetMet: 0.733 ± 0.026	0.722MetAsn: 0.722 ± 0.024	1.541MetPro: 1.541 ± 0.036	0.91MetGln: 0.91 ± 0.03	1.796MetArg: 1.796 ± 0.041	1.49MetSer: 1.49 ± 0.035	1.603MetThr: 1.603 ± 0.037	1.987MetVal: 1.987 ± 0.047	0.228MetTrp: 0.228 ± 0.013	0.268MetTyr: 0.268 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	3.554AsnAla: 3.554 ± 0.059	0.253AsnCys: 0.253 ± 0.015	1.339AsnAsp: 1.339 ± 0.037	1.078AsnGlu: 1.078 ± 0.032	1.089AsnPhe: 1.089 ± 0.031	2.676AsnGly: 2.676 ± 0.068	0.571AsnHis: 0.571 ± 0.022	1.385AsnIle: 1.385 ± 0.038	0.774AsnLys: 0.774 ± 0.029	2.91AsnLeu: 2.91 ± 0.062	0.603AsnMet: 0.603 ± 0.022	0.832AsnAsn: 0.832 ± 0.035	1.968AsnPro: 1.968 ± 0.044	0.903AsnGln: 0.903 ± 0.033	1.81AsnArg: 1.81 ± 0.037	1.495AsnSer: 1.495 ± 0.04	1.427AsnThr: 1.427 ± 0.04	1.939AsnVal: 1.939 ± 0.046	0.497AsnTrp: 0.497 ± 0.02	0.845AsnTyr: 0.845 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	7.276ProAla: 7.276 ± 0.086	0.385ProCys: 0.385 ± 0.019	3.615ProAsp: 3.615 ± 0.057	3.379ProGlu: 3.379 ± 0.052	1.92ProPhe: 1.92 ± 0.045	3.294ProGly: 3.294 ± 0.053	1.108ProHis: 1.108 ± 0.036	2.192ProIle: 2.192 ± 0.041	1.807ProLys: 1.807 ± 0.045	5.167ProLeu: 5.167 ± 0.066	1.385ProMet: 1.385 ± 0.034	1.318ProAsn: 1.318 ± 0.034	2.443ProPro: 2.443 ± 0.063	2.166ProGln: 2.166 ± 0.044	2.829ProArg: 2.829 ± 0.057	2.862ProSer: 2.862 ± 0.048	2.509ProThr: 2.509 ± 0.044	4.406ProVal: 4.406 ± 0.074	0.769ProTrp: 0.769 ± 0.027	1.186ProTyr: 1.186 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	5.289GlnAla: 5.289 ± 0.086	0.251GlnCys: 0.251 ± 0.015	1.796GlnAsp: 1.796 ± 0.038	1.515GlnGlu: 1.515 ± 0.037	1.255GlnPhe: 1.255 ± 0.033	3.252GlnGly: 3.252 ± 0.046	0.768GlnHis: 0.768 ± 0.023	1.986GlnIle: 1.986 ± 0.045	1.133GlnLys: 1.133 ± 0.033	3.33GlnLeu: 3.33 ± 0.055	1.039GlnMet: 1.039 ± 0.031	1.024GlnAsn: 1.024 ± 0.035	2.043GlnPro: 2.043 ± 0.045	1.441GlnGln: 1.441 ± 0.039	2.537GlnArg: 2.537 ± 0.048	2.144GlnSer: 2.144 ± 0.043	1.91GlnThr: 1.91 ± 0.046	2.823GlnVal: 2.823 ± 0.059	0.669GlnTrp: 0.669 ± 0.024	0.701GlnTyr: 0.701 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	7.768ArgAla: 7.768 ± 0.09	0.475ArgCys: 0.475 ± 0.02	3.539ArgAsp: 3.539 ± 0.049	3.693ArgGlu: 3.693 ± 0.069	2.759ArgPhe: 2.759 ± 0.056	4.631ArgGly: 4.631 ± 0.068	1.711ArgHis: 1.711 ± 0.035	3.577ArgIle: 3.577 ± 0.054	2.288ArgLys: 2.288 ± 0.047	7.646ArgLeu: 7.646 ± 0.1	1.856ArgMet: 1.856 ± 0.041	1.852ArgAsn: 1.852 ± 0.046	3.176ArgPro: 3.176 ± 0.055	2.693ArgGln: 2.693 ± 0.057	4.707ArgArg: 4.707 ± 0.076	3.294ArgSer: 3.294 ± 0.057	2.496ArgThr: 2.496 ± 0.046	4.367ArgVal: 4.367 ± 0.064	1.159ArgTrp: 1.159 ± 0.035	1.864ArgTyr: 1.864 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	7.146SerAla: 7.146 ± 0.093	0.431SerCys: 0.431 ± 0.019	2.934SerAsp: 2.934 ± 0.052	2.498SerGlu: 2.498 ± 0.058	2.263SerPhe: 2.263 ± 0.049	5.651SerGly: 5.651 ± 0.084	1.098SerHis: 1.098 ± 0.027	2.564SerIle: 2.564 ± 0.047	1.494SerLys: 1.494 ± 0.036	5.796SerLeu: 5.796 ± 0.075	1.272SerMet: 1.272 ± 0.036	1.53SerAsn: 1.53 ± 0.045	2.881SerPro: 2.881 ± 0.05	1.835SerGln: 1.835 ± 0.045	3.199SerArg: 3.199 ± 0.052	3.21SerSer: 3.21 ± 0.081	2.984SerThr: 2.984 ± 0.065	3.839SerVal: 3.839 ± 0.056	0.802SerTrp: 0.802 ± 0.025	1.486SerTyr: 1.486 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	6.148ThrAla: 6.148 ± 0.082	0.455ThrCys: 0.455 ± 0.021	2.372ThrAsp: 2.372 ± 0.046	2.009ThrGlu: 2.009 ± 0.043	1.703ThrPhe: 1.703 ± 0.039	5.241ThrGly: 5.241 ± 0.08	1.002ThrHis: 1.002 ± 0.029	2.717ThrIle: 2.717 ± 0.046	1.5ThrLys: 1.5 ± 0.04	5.52ThrLeu: 5.52 ± 0.07	1.224ThrMet: 1.224 ± 0.029	1.5ThrAsn: 1.5 ± 0.046	3.288ThrPro: 3.288 ± 0.055	1.902ThrGln: 1.902 ± 0.043	3.107ThrArg: 3.107 ± 0.054	3.061ThrSer: 3.061 ± 0.066	2.913ThrThr: 2.913 ± 0.071	3.664ThrVal: 3.664 ± 0.059	0.643ThrTrp: 0.643 ± 0.023	1.463ThrTyr: 1.463 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	9.683ValAla: 9.683 ± 0.089	0.541ValCys: 0.541 ± 0.023	4.088ValAsp: 4.088 ± 0.063	4.474ValGlu: 4.474 ± 0.073	2.145ValPhe: 2.145 ± 0.04	5.408ValGly: 5.408 ± 0.079	1.233ValHis: 1.233 ± 0.035	3.339ValIle: 3.339 ± 0.055	2.416ValLys: 2.416 ± 0.047	7.119ValLeu: 7.119 ± 0.076	1.87ValMet: 1.87 ± 0.035	2.203ValAsn: 2.203 ± 0.054	3.763ValPro: 3.763 ± 0.062	2.361ValGln: 2.361 ± 0.039	4.024ValArg: 4.024 ± 0.067	4.237ValSer: 4.237 ± 0.06	4.2ValThr: 4.2 ± 0.066	5.59ValVal: 5.59 ± 0.079	0.972ValTrp: 0.972 ± 0.03	1.326ValTyr: 1.326 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.611TrpAla: 1.611 ± 0.042	0.138TrpCys: 0.138 ± 0.012	0.697TrpAsp: 0.697 ± 0.026	0.57TrpGlu: 0.57 ± 0.022	0.571TrpPhe: 0.571 ± 0.023	1.106TrpGly: 1.106 ± 0.034	0.399TrpHis: 0.399 ± 0.019	0.622TrpIle: 0.622 ± 0.025	0.527TrpLys: 0.527 ± 0.021	1.893TrpLeu: 1.893 ± 0.049	0.373TrpMet: 0.373 ± 0.018	0.463TrpAsn: 0.463 ± 0.02	0.775TrpPro: 0.775 ± 0.029	0.781TrpGln: 0.781 ± 0.026	1.384TrpArg: 1.384 ± 0.033	0.88TrpSer: 0.88 ± 0.03	0.742TrpThr: 0.742 ± 0.028	0.952TrpVal: 0.952 ± 0.029	0.29TrpTrp: 0.29 ± 0.017	0.341TrpTyr: 0.341 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.98TyrAla: 2.98 ± 0.055	0.24TyrCys: 0.24 ± 0.014	1.53TyrAsp: 1.53 ± 0.035	1.094TyrGlu: 1.094 ± 0.031	0.862TyrPhe: 0.862 ± 0.029	2.321TyrGly: 2.321 ± 0.04	0.592TyrHis: 0.592 ± 0.019	0.948TyrIle: 0.948 ± 0.026	0.661TyrLys: 0.661 ± 0.026	2.167TyrLeu: 2.167 ± 0.044	0.449TyrMet: 0.449 ± 0.021	0.862TyrAsn: 0.862 ± 0.031	1.087TyrPro: 1.087 ± 0.03	0.87TyrGln: 0.87 ± 0.028	1.854TyrArg: 1.854 ± 0.036	1.321TyrSer: 1.321 ± 0.037	1.291TyrThr: 1.291 ± 0.035	1.619TyrVal: 1.619 ± 0.036	0.369TyrTrp: 0.369 ± 0.017	0.716TyrTyr: 0.716 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3564 proteins (1221789 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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