Lacipirellula parvula
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6194 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5K7XFQ9|A0A5K7XFQ9_9BACT Uncharacterized protein OS=Lacipirellula parvula OX=2650471 GN=PLANPX_2685 PE=4 SV=1
MM1 pKa = 7.55 PNIRR5 pKa = 11.84 EE6 pKa = 4.11 TWQDD10 pKa = 3.34 PDD12 pKa = 4.6 EE13 pKa = 5.08 DD14 pKa = 4.4 WDD16 pKa = 5.38 DD17 pKa = 3.94 EE18 pKa = 5.32 DD19 pKa = 6.98 DD20 pKa = 5.24 DD21 pKa = 5.7 FEE23 pKa = 4.74 EE24 pKa = 6.01 HH25 pKa = 7.83 DD26 pKa = 4.63 EE27 pKa = 4.62 DD28 pKa = 4.61 DD29 pKa = 4.0 QEE31 pKa = 4.62 EE32 pKa = 4.41 PTVTCPYY39 pKa = 9.98 CRR41 pKa = 11.84 AEE43 pKa = 4.01 VWEE46 pKa = 4.71 EE47 pKa = 3.32 ASQCPKK53 pKa = 10.2 CGEE56 pKa = 4.15 YY57 pKa = 10.75 LSLEE61 pKa = 4.59 DD62 pKa = 4.81 GRR64 pKa = 11.84 ARR66 pKa = 11.84 HH67 pKa = 5.15 QWQPRR72 pKa = 11.84 WIVLTAALLLGLILLGYY89 pKa = 10.15 LSSLGGG95 pKa = 3.38
Molecular weight: 11.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.753
IPC2_protein 3.846
IPC_protein 3.795
Toseland 3.605
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.516
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.075
Thurlkill 3.656
EMBOSS 3.706
Sillero 3.923
Patrickios 1.863
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|A0A5K7XFT5|A0A5K7XFT5_9BACT Uncharacterized protein OS=Lacipirellula parvula OX=2650471 GN=PLANPX_4519 PE=4 SV=1
MM1 pKa = 7.08 QRR3 pKa = 11.84 AQGGRR8 pKa = 11.84 VAFWQHH14 pKa = 5.26 RR15 pKa = 11.84 RR16 pKa = 11.84 QLGPFSGLFSPGWRR30 pKa = 11.84 RR31 pKa = 11.84 GAAGQATLSPMSGRR45 pKa = 11.84 SAAGMRR51 pKa = 11.84 VALFAGAFTRR61 pKa = 11.84 IQQ63 pKa = 3.07
Molecular weight: 6.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.502
IPC2_protein 11.125
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.398
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.149
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.179
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6194
0
6194
1979840
29
8054
319.6
34.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.84 ± 0.044
1.055 ± 0.014
5.64 ± 0.029
5.928 ± 0.035
3.643 ± 0.019
8.258 ± 0.048
2.032 ± 0.016
4.647 ± 0.022
3.362 ± 0.028
9.692 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.124 ± 0.015
3.257 ± 0.03
5.34 ± 0.03
3.744 ± 0.021
6.629 ± 0.037
6.066 ± 0.031
5.539 ± 0.035
7.204 ± 0.024
1.552 ± 0.015
2.448 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here