Itaporanga virus
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1DW07|A0A4V1DW07_9VIRU Replicase OS=Itaporanga virus OX=629735 PE=4 SV=2
MM1 pKa = 7.11 MRR3 pKa = 11.84 FFHH6 pKa = 7.32 PEE8 pKa = 3.38 MPEE11 pKa = 3.64 YY12 pKa = 11.01 SFDD15 pKa = 3.6 YY16 pKa = 10.1 SKK18 pKa = 11.42 EE19 pKa = 3.86 GGRR22 pKa = 11.84 VNVLYY27 pKa = 10.86 VNKK30 pKa = 10.13 FEE32 pKa = 4.22 WTEE35 pKa = 4.22 VPASYY40 pKa = 11.02 HH41 pKa = 5.66 MGSLIPLKK49 pKa = 10.31 FVSLSRR55 pKa = 11.84 KK56 pKa = 9.76 KK57 pKa = 10.74 FMNLKK62 pKa = 9.85 EE63 pKa = 4.2 FYY65 pKa = 10.78 DD66 pKa = 4.5 RR67 pKa = 11.84 DD68 pKa = 4.11 TLPLLWGDD76 pKa = 3.99 CKK78 pKa = 10.76 GSQVMGPSLNCFDD91 pKa = 5.47 EE92 pKa = 4.68 MIKK95 pKa = 9.62 MLASIPLNDD104 pKa = 4.03 FLAPSRR110 pKa = 11.84 GHH112 pKa = 5.29 TAHH115 pKa = 7.17 GISWPTGSPDD125 pKa = 3.33 LNYY128 pKa = 9.97 IVYY131 pKa = 10.02 SSKK134 pKa = 10.96 DD135 pKa = 3.51 INPEE139 pKa = 3.83 SYY141 pKa = 10.69 YY142 pKa = 10.74 SRR144 pKa = 11.84 CHH146 pKa = 5.0 VASMLMNGAKK156 pKa = 10.31 GNPSLDD162 pKa = 3.83 LACVALHH169 pKa = 5.93 KK170 pKa = 10.32 KK171 pKa = 8.69 IRR173 pKa = 11.84 KK174 pKa = 8.42 AAADD178 pKa = 3.42 MGVSPEE184 pKa = 4.18 LFSGRR189 pKa = 11.84 NLVKK193 pKa = 10.56 DD194 pKa = 3.78 VACVQVISVLRR205 pKa = 11.84 GAEE208 pKa = 3.43 MDD210 pKa = 3.19 QRR212 pKa = 11.84 VFGKK216 pKa = 10.64 GDD218 pKa = 3.65 NLVDD222 pKa = 3.56 WLVEE226 pKa = 3.67 CAARR230 pKa = 11.84 IRR232 pKa = 11.84 TSDD235 pKa = 3.64 PGCLGDD241 pKa = 4.86 EE242 pKa = 4.1 IGFGPRR248 pKa = 11.84 CRR250 pKa = 11.84 AIDD253 pKa = 3.98 DD254 pKa = 4.3 LDD256 pKa = 3.9 RR257 pKa = 11.84 CLEE260 pKa = 4.33 GNDD263 pKa = 4.2 SDD265 pKa = 5.76 FSDD268 pKa = 5.13 DD269 pKa = 4.51 SSDD272 pKa = 4.1 DD273 pKa = 3.42 EE274 pKa = 4.46 AAIAASNLSSVEE286 pKa = 3.85
Molecular weight: 31.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.947
IPC2_protein 5.016
IPC_protein 4.978
Toseland 4.889
ProMoST 5.054
Dawson 4.978
Bjellqvist 5.13
Wikipedia 4.889
Rodwell 4.876
Grimsley 4.8
Solomon 4.978
Lehninger 4.94
Nozaki 5.105
DTASelect 5.308
Thurlkill 4.902
EMBOSS 4.927
Sillero 5.156
Patrickios 3.872
IPC_peptide 4.978
IPC2_peptide 5.156
IPC2.peptide.svr19 5.08
Protein with the highest isoelectric point:
>tr|A0A4P8D7T3|A0A4P8D7T3_9VIRU Glycoprotein OS=Itaporanga virus OX=629735 PE=4 SV=1
MM1 pKa = 7.65 SDD3 pKa = 3.5 YY4 pKa = 11.5 ARR6 pKa = 11.84 IALDD10 pKa = 4.11 FYY12 pKa = 11.25 EE13 pKa = 4.79 NNPPTTNDD21 pKa = 2.65 IAEE24 pKa = 4.28 FVSLFAYY31 pKa = 9.81 QGYY34 pKa = 9.1 DD35 pKa = 2.58 ARR37 pKa = 11.84 TVVRR41 pKa = 11.84 LVTEE45 pKa = 4.32 RR46 pKa = 11.84 GGGNWQEE53 pKa = 3.92 DD54 pKa = 4.1 VKK56 pKa = 11.21 RR57 pKa = 11.84 LIVLAATRR65 pKa = 11.84 GNRR68 pKa = 11.84 VNKK71 pKa = 9.29 MKK73 pKa = 11.02 AKK75 pKa = 10.07 MSDD78 pKa = 3.05 KK79 pKa = 11.15 GKK81 pKa = 11.3 GEE83 pKa = 3.96 LTKK86 pKa = 10.83 LVSTYY91 pKa = 10.65 KK92 pKa = 10.61 LKK94 pKa = 10.77 EE95 pKa = 4.07 GNPGRR100 pKa = 11.84 EE101 pKa = 4.3 DD102 pKa = 3.24 LTLSRR107 pKa = 11.84 VACAFATWTCTCITSIAEE125 pKa = 4.02 YY126 pKa = 10.71 LPVTGSTMDD135 pKa = 3.51 SYY137 pKa = 11.8 SPNYY141 pKa = 8.48 PRR143 pKa = 11.84 TMMHH147 pKa = 6.89 PAFGGLIDD155 pKa = 3.99 LTLPEE160 pKa = 5.24 AEE162 pKa = 4.39 TLVAAHH168 pKa = 6.56 SLYY171 pKa = 10.94 LNRR174 pKa = 11.84 FAQVINPGLRR184 pKa = 11.84 GKK186 pKa = 10.57 SKK188 pKa = 11.36 DD189 pKa = 3.51 EE190 pKa = 3.86 IQATFQQPLNAAANSSFITSEE211 pKa = 3.95 NKK213 pKa = 9.94 RR214 pKa = 11.84 KK215 pKa = 9.52 FLTKK219 pKa = 10.42 FMLIDD224 pKa = 3.88 SNLKK228 pKa = 7.46 VTKK231 pKa = 10.36 SVADD235 pKa = 3.35 AAKK238 pKa = 10.18 AYY240 pKa = 9.06 KK241 pKa = 10.33 ALNN244 pKa = 3.6
Molecular weight: 27.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.665
IPC2_protein 8.58
IPC_protein 8.507
Toseland 9.121
ProMoST 8.975
Dawson 9.443
Bjellqvist 9.238
Wikipedia 9.604
Rodwell 9.648
Grimsley 9.502
Solomon 9.516
Lehninger 9.472
Nozaki 9.311
DTASelect 9.165
Thurlkill 9.282
EMBOSS 9.575
Sillero 9.428
Patrickios 4.698
IPC_peptide 9.502
IPC2_peptide 7.863
IPC2.peptide.svr19 7.7
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3890
244
2094
972.5
108.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.578 ± 0.987
2.802 ± 0.876
5.604 ± 0.564
6.607 ± 0.807
4.576 ± 0.121
6.375 ± 0.442
2.262 ± 0.274
6.298 ± 0.603
6.35 ± 0.42
8.612 ± 0.249
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.828 ± 0.649
3.702 ± 0.491
4.293 ± 0.363
2.931 ± 0.444
5.193 ± 0.392
9.486 ± 1.009
5.681 ± 0.584
6.735 ± 0.273
1.183 ± 0.089
2.905 ± 0.295
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here