Mycoplasma phage phiMFV1

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6GYZ6|Q6GYZ6_9VIRU ABC transmembrane type-1 domain-containing protein (Fragment) OS=Mycoplasma phage phiMFV1 OX=280702 PE=4 SV=1
MM1 pKa = 7.7LNNKK5 pKa = 9.43CDD7 pKa = 3.68FCHH10 pKa = 7.42KK11 pKa = 10.17RR12 pKa = 11.84FQPKK16 pKa = 9.45KK17 pKa = 10.15EE18 pKa = 3.8RR19 pKa = 11.84YY20 pKa = 7.88FWGNLVVCSEE30 pKa = 4.28EE31 pKa = 4.36CLEE34 pKa = 4.41QVSCYY39 pKa = 10.62ASEE42 pKa = 4.19IDD44 pKa = 4.8LNCEE48 pKa = 4.0GEE50 pKa = 4.19NNEE53 pKa = 4.24

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5QGJ7|Q5QGJ7_9VIRU HtpE OS=Mycoplasma phage phiMFV1 OX=280702 GN=htpE PE=4 SV=1
MM1 pKa = 7.75LIYY4 pKa = 10.73SNPKK8 pKa = 9.77PKK10 pKa = 8.35TTLQLSPKK18 pKa = 9.94SFVINDD24 pKa = 3.72SKK26 pKa = 11.57KK27 pKa = 10.37QMNPFLRR34 pKa = 11.84GFLSFLHH41 pKa = 5.85VALDD45 pKa = 3.58IGASFLGSFVNTFAPGLGLVIEE67 pKa = 4.84YY68 pKa = 10.61GLSTALDD75 pKa = 3.93LGFNAAINYY84 pKa = 9.23GNINWQDD91 pKa = 3.48FGISAGINAIPFVTRR106 pKa = 11.84GIRR109 pKa = 11.84YY110 pKa = 8.16AANTHH115 pKa = 5.19KK116 pKa = 9.74TNKK119 pKa = 9.34FFQLAKK125 pKa = 9.87NLKK128 pKa = 10.14KK129 pKa = 10.57EE130 pKa = 4.16GQEE133 pKa = 3.9EE134 pKa = 4.24LSEE137 pKa = 4.77KK138 pKa = 8.1ITKK141 pKa = 9.63IAANYY146 pKa = 10.12SEE148 pKa = 5.48LGVDD152 pKa = 3.03KK153 pKa = 11.23AKK155 pKa = 10.97FIDD158 pKa = 3.84QKK160 pKa = 11.72SLLKK164 pKa = 10.66KK165 pKa = 9.73EE166 pKa = 4.58LKK168 pKa = 10.59LFNNDD173 pKa = 2.37SFLRR177 pKa = 11.84TLSDD181 pKa = 3.27NKK183 pKa = 10.54NIYY186 pKa = 10.25YY187 pKa = 10.28FLNKK191 pKa = 9.47KK192 pKa = 7.82VQNIQKK198 pKa = 10.15AFTHH202 pKa = 5.61LRR204 pKa = 11.84SMVSLLTSPSYY215 pKa = 10.23LAKK218 pKa = 10.51KK219 pKa = 10.44AVDD222 pKa = 3.59TVFKK226 pKa = 10.77HH227 pKa = 5.72PKK229 pKa = 10.11AYY231 pKa = 10.6LNKK234 pKa = 9.92QLGKK238 pKa = 8.76FTKK241 pKa = 10.25LLKK244 pKa = 9.61NTFKK248 pKa = 10.91SKK250 pKa = 10.24PIKK253 pKa = 10.37KK254 pKa = 9.27VVKK257 pKa = 10.05KK258 pKa = 10.0ASNYY262 pKa = 8.55IWIDD266 pKa = 3.06HH267 pKa = 5.81SSWLEE272 pKa = 3.48RR273 pKa = 11.84MSINKK278 pKa = 8.7VNNPWDD284 pKa = 3.31IKK286 pKa = 10.93AINAVLYY293 pKa = 8.99FKK295 pKa = 11.16KK296 pKa = 10.48NATKK300 pKa = 10.49SKK302 pKa = 10.22KK303 pKa = 9.23NPKK306 pKa = 9.45GKK308 pKa = 10.41EE309 pKa = 3.76PVRR312 pKa = 11.84LWNKK316 pKa = 10.63SIDD319 pKa = 3.81EE320 pKa = 4.7LIKK323 pKa = 10.48LVCAKK328 pKa = 10.74SPGRR332 pKa = 11.84YY333 pKa = 8.67YY334 pKa = 11.05LNNIAYY340 pKa = 8.23GHH342 pKa = 5.62IVGRR346 pKa = 11.84FIRR349 pKa = 11.84KK350 pKa = 9.02FEE352 pKa = 3.97MFQAVPIHH360 pKa = 6.62PIFSNLIGTSLFTFRR375 pKa = 11.84TINSLIMNFKK385 pKa = 7.92RR386 pKa = 11.84QKK388 pKa = 9.49WVWLEE393 pKa = 3.95DD394 pKa = 3.29KK395 pKa = 11.04NIILNEE401 pKa = 3.68IVKK404 pKa = 10.0GVKK407 pKa = 10.1EE408 pKa = 4.02NVFKK412 pKa = 10.75GWRR415 pKa = 11.84APGFQFISSMARR427 pKa = 11.84SWATGNVGYY436 pKa = 7.45QTKK439 pKa = 10.43AALRR443 pKa = 11.84WANKK447 pKa = 9.58KK448 pKa = 10.56AFTSKK453 pKa = 7.84TQKK456 pKa = 8.36TNYY459 pKa = 8.17WVNHH463 pKa = 4.26WVKK466 pKa = 10.94NKK468 pKa = 10.01YY469 pKa = 9.78KK470 pKa = 10.57KK471 pKa = 10.53

Molecular weight: 54.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17

0

17

4901

53

615

288.3

33.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.264 ± 0.344

0.673 ± 0.174

4.713 ± 0.351

7.631 ± 0.558

6.06 ± 0.37

3.632 ± 0.243

1.041 ± 0.178

8.835 ± 0.443

11.589 ± 0.598

10.161 ± 0.496

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.673 ± 0.108

9.529 ± 0.537

2.571 ± 0.27

3.836 ± 0.236

2.346 ± 0.238

6.305 ± 0.476

4.652 ± 0.28

4.55 ± 0.312

1.51 ± 0.187

4.428 ± 0.288
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski