Amino acid dipepetide frequency for Betaproteobacteria bacterium MOLA814

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.608AlaAla: 13.608 ± 0.163	1.42AlaCys: 1.42 ± 0.045	5.729AlaAsp: 5.729 ± 0.084	4.337AlaGlu: 4.337 ± 0.072	3.98AlaPhe: 3.98 ± 0.066	9.054AlaGly: 9.054 ± 0.107	3.299AlaHis: 3.299 ± 0.061	5.683AlaIle: 5.683 ± 0.09	4.682AlaLys: 4.682 ± 0.09	13.62AlaLeu: 13.62 ± 0.144	4.085AlaMet: 4.085 ± 0.064	3.756AlaAsn: 3.756 ± 0.075	4.655AlaPro: 4.655 ± 0.085	6.374AlaGln: 6.374 ± 0.098	5.766AlaArg: 5.766 ± 0.096	7.361AlaSer: 7.361 ± 0.095	6.52AlaThr: 6.52 ± 0.1	8.947AlaVal: 8.947 ± 0.118	1.824AlaTrp: 1.824 ± 0.048	2.624AlaTyr: 2.624 ± 0.057	0.001AlaXaa: 0.001 ± 0.001
Cys	1.368CysAla: 1.368 ± 0.044	0.147CysCys: 0.147 ± 0.013	0.635CysAsp: 0.635 ± 0.027	0.529CysGlu: 0.529 ± 0.026	0.356CysPhe: 0.356 ± 0.021	1.024CysGly: 1.024 ± 0.037	0.286CysHis: 0.286 ± 0.02	0.507CysIle: 0.507 ± 0.027	0.323CysLys: 0.323 ± 0.019	0.945CysLeu: 0.945 ± 0.035	0.318CysMet: 0.318 ± 0.019	0.343CysAsn: 0.343 ± 0.021	0.53CysPro: 0.53 ± 0.024	0.364CysGln: 0.364 ± 0.022	0.456CysArg: 0.456 ± 0.022	0.597CysSer: 0.597 ± 0.027	0.65CysThr: 0.65 ± 0.03	0.967CysVal: 0.967 ± 0.033	0.137CysTrp: 0.137 ± 0.012	0.223CysTyr: 0.223 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	6.319AspAla: 6.319 ± 0.104	0.513AspCys: 0.513 ± 0.027	2.911AspAsp: 2.911 ± 0.07	2.847AspGlu: 2.847 ± 0.066	2.075AspPhe: 2.075 ± 0.05	4.075AspGly: 4.075 ± 0.072	1.197AspHis: 1.197 ± 0.036	3.015AspIle: 3.015 ± 0.057	2.296AspLys: 2.296 ± 0.055	5.33AspLeu: 5.33 ± 0.082	1.761AspMet: 1.761 ± 0.053	1.857AspAsn: 1.857 ± 0.047	2.246AspPro: 2.246 ± 0.052	1.937AspGln: 1.937 ± 0.046	2.346AspArg: 2.346 ± 0.057	2.622AspSer: 2.622 ± 0.056	3.043AspThr: 3.043 ± 0.072	4.669AspVal: 4.669 ± 0.084	0.95AspTrp: 0.95 ± 0.034	1.376AspTyr: 1.376 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	5.38GluAla: 5.38 ± 0.093	0.376GluCys: 0.376 ± 0.018	2.009GluAsp: 2.009 ± 0.049	1.918GluGlu: 1.918 ± 0.063	1.643GluPhe: 1.643 ± 0.047	2.951GluGly: 2.951 ± 0.062	1.345GluHis: 1.345 ± 0.039	2.346GluIle: 2.346 ± 0.062	1.698GluLys: 1.698 ± 0.052	5.339GluLeu: 5.339 ± 0.078	1.237GluMet: 1.237 ± 0.035	1.119GluAsn: 1.119 ± 0.035	2.16GluPro: 2.16 ± 0.049	2.565GluGln: 2.565 ± 0.058	3.406GluArg: 3.406 ± 0.083	2.477GluSer: 2.477 ± 0.059	2.473GluThr: 2.473 ± 0.05	3.711GluVal: 3.711 ± 0.086	0.738GluTrp: 0.738 ± 0.029	0.921GluTyr: 0.921 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	4.017PheAla: 4.017 ± 0.076	0.448PheCys: 0.448 ± 0.02	2.553PheAsp: 2.553 ± 0.054	2.389PheGlu: 2.389 ± 0.063	1.438PhePhe: 1.438 ± 0.044	3.282PheGly: 3.282 ± 0.059	0.729PheHis: 0.729 ± 0.03	1.758PheIle: 1.758 ± 0.051	1.645PheLys: 1.645 ± 0.043	2.855PheLeu: 2.855 ± 0.063	0.974PheMet: 0.974 ± 0.032	1.405PheAsn: 1.405 ± 0.04	1.39PhePro: 1.39 ± 0.044	1.07PheGln: 1.07 ± 0.036	1.361PheArg: 1.361 ± 0.039	2.314PheSer: 2.314 ± 0.055	2.039PheThr: 2.039 ± 0.055	3.006PheVal: 3.006 ± 0.07	0.518PheTrp: 0.518 ± 0.027	0.978PheTyr: 0.978 ± 0.038	0.001PheXaa: 0.001 ± 0.001
Gly	8.082GlyAla: 8.082 ± 0.131	0.964GlyCys: 0.964 ± 0.036	4.101GlyAsp: 4.101 ± 0.072	3.804GlyGlu: 3.804 ± 0.072	3.342GlyPhe: 3.342 ± 0.071	6.161GlyGly: 6.161 ± 0.096	2.082GlyHis: 2.082 ± 0.043	4.111GlyIle: 4.111 ± 0.079	3.292GlyLys: 3.292 ± 0.073	8.74GlyLeu: 8.74 ± 0.129	2.61GlyMet: 2.61 ± 0.062	2.332GlyAsn: 2.332 ± 0.054	2.572GlyPro: 2.572 ± 0.055	3.613GlyGln: 3.613 ± 0.065	3.914GlyArg: 3.914 ± 0.078	4.373GlySer: 4.373 ± 0.08	3.963GlyThr: 3.963 ± 0.07	7.073GlyVal: 7.073 ± 0.092	1.298GlyTrp: 1.298 ± 0.041	2.124GlyTyr: 2.124 ± 0.052	0.005GlyXaa: 0.005 ± 0.002
His	3.135HisAla: 3.135 ± 0.065	0.329HisCys: 0.329 ± 0.023	1.213HisAsp: 1.213 ± 0.044	0.997HisGlu: 0.997 ± 0.034	0.885HisPhe: 0.885 ± 0.032	2.021HisGly: 2.021 ± 0.051	0.763HisHis: 0.763 ± 0.034	1.56HisIle: 1.56 ± 0.044	0.828HisLys: 0.828 ± 0.029	2.377HisLeu: 2.377 ± 0.06	0.829HisMet: 0.829 ± 0.033	0.903HisAsn: 0.903 ± 0.029	1.356HisPro: 1.356 ± 0.038	0.966HisGln: 0.966 ± 0.03	1.178HisArg: 1.178 ± 0.036	1.418HisSer: 1.418 ± 0.045	1.795HisThr: 1.795 ± 0.05	2.117HisVal: 2.117 ± 0.055	0.44HisTrp: 0.44 ± 0.022	0.703HisTyr: 0.703 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.184IleAla: 6.184 ± 0.093	0.489IleCys: 0.489 ± 0.024	3.254IleAsp: 3.254 ± 0.049	3.142IleGlu: 3.142 ± 0.057	1.439IlePhe: 1.439 ± 0.044	4.195IleGly: 4.195 ± 0.089	1.02IleHis: 1.02 ± 0.037	1.862IleIle: 1.862 ± 0.054	2.129IleLys: 2.129 ± 0.054	3.355IleLeu: 3.355 ± 0.076	1.056IleMet: 1.056 ± 0.039	1.983IleAsn: 1.983 ± 0.046	2.067IlePro: 2.067 ± 0.056	1.658IleGln: 1.658 ± 0.046	2.375IleArg: 2.375 ± 0.054	2.852IleSer: 2.852 ± 0.064	2.963IleThr: 2.963 ± 0.068	3.524IleVal: 3.524 ± 0.063	0.56IleTrp: 0.56 ± 0.025	1.133IleTyr: 1.133 ± 0.039	0.002IleXaa: 0.002 ± 0.002
Lys	4.553LysAla: 4.553 ± 0.083	0.204LysCys: 0.204 ± 0.017	1.925LysAsp: 1.925 ± 0.051	1.537LysGlu: 1.537 ± 0.051	1.042LysPhe: 1.042 ± 0.043	2.77LysGly: 2.77 ± 0.07	0.955LysHis: 0.955 ± 0.034	1.611LysIle: 1.611 ± 0.048	1.619LysLys: 1.619 ± 0.064	3.971LysLeu: 3.971 ± 0.064	0.895LysMet: 0.895 ± 0.032	1.147LysAsn: 1.147 ± 0.039	2.315LysPro: 2.315 ± 0.066	1.689LysGln: 1.689 ± 0.048	2.759LysArg: 2.759 ± 0.055	2.24LysSer: 2.24 ± 0.051	2.314LysThr: 2.314 ± 0.055	2.702LysVal: 2.702 ± 0.064	0.479LysTrp: 0.479 ± 0.023	0.699LysTyr: 0.699 ± 0.032	0.0LysXaa: 0.0 ± 0.0
Leu	12.919LeuAla: 12.919 ± 0.132	1.205LeuCys: 1.205 ± 0.039	5.658LeuAsp: 5.658 ± 0.092	4.544LeuGlu: 4.544 ± 0.086	3.514LeuPhe: 3.514 ± 0.083	8.464LeuGly: 8.464 ± 0.113	2.513LeuHis: 2.513 ± 0.069	4.668LeuIle: 4.668 ± 0.086	3.944LeuLys: 3.944 ± 0.079	10.607LeuLeu: 10.607 ± 0.151	3.021LeuMet: 3.021 ± 0.064	3.618LeuAsn: 3.618 ± 0.073	5.28LeuPro: 5.28 ± 0.073	4.468LeuGln: 4.468 ± 0.077	6.073LeuArg: 6.073 ± 0.1	7.163LeuSer: 7.163 ± 0.109	5.567LeuThr: 5.567 ± 0.088	7.934LeuVal: 7.934 ± 0.101	1.355LeuTrp: 1.355 ± 0.046	1.974LeuTyr: 1.974 ± 0.054	0.001LeuXaa: 0.001 ± 0.001
Met	3.716MetAla: 3.716 ± 0.071	0.28MetCys: 0.28 ± 0.019	1.327MetAsp: 1.327 ± 0.041	0.945MetGlu: 0.945 ± 0.034	0.892MetPhe: 0.892 ± 0.035	2.539MetGly: 2.539 ± 0.057	0.751MetHis: 0.751 ± 0.032	0.989MetIle: 0.989 ± 0.034	1.014MetLys: 1.014 ± 0.033	3.229MetLeu: 3.229 ± 0.064	0.7MetMet: 0.7 ± 0.026	1.063MetAsn: 1.063 ± 0.04	1.658MetPro: 1.658 ± 0.047	1.344MetGln: 1.344 ± 0.039	1.876MetArg: 1.876 ± 0.048	2.049MetSer: 2.049 ± 0.054	1.983MetThr: 1.983 ± 0.046	2.278MetVal: 2.278 ± 0.064	0.366MetTrp: 0.366 ± 0.02	0.506MetTyr: 0.506 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	3.978AsnAla: 3.978 ± 0.071	0.376AsnCys: 0.376 ± 0.023	1.746AsnAsp: 1.746 ± 0.039	1.43AsnGlu: 1.43 ± 0.043	1.173AsnPhe: 1.173 ± 0.044	2.494AsnGly: 2.494 ± 0.053	0.735AsnHis: 0.735 ± 0.029	1.658AsnIle: 1.658 ± 0.048	1.331AsnLys: 1.331 ± 0.039	3.022AsnLeu: 3.022 ± 0.054	0.946AsnMet: 0.946 ± 0.031	1.399AsnAsn: 1.399 ± 0.045	1.972AsnPro: 1.972 ± 0.043	1.219AsnGln: 1.219 ± 0.037	1.61AsnArg: 1.61 ± 0.048	1.75AsnSer: 1.75 ± 0.043	2.138AsnThr: 2.138 ± 0.049	2.388AsnVal: 2.388 ± 0.062	0.591AsnTrp: 0.591 ± 0.03	0.849AsnTyr: 0.849 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	5.053ProAla: 5.053 ± 0.075	0.383ProCys: 0.383 ± 0.022	2.708ProAsp: 2.708 ± 0.06	2.553ProGlu: 2.553 ± 0.053	1.681ProPhe: 1.681 ± 0.046	3.392ProGly: 3.392 ± 0.064	1.241ProHis: 1.241 ± 0.039	2.175ProIle: 2.175 ± 0.049	1.815ProLys: 1.815 ± 0.042	4.467ProLeu: 4.467 ± 0.078	1.482ProMet: 1.482 ± 0.046	1.717ProAsn: 1.717 ± 0.043	1.919ProPro: 1.919 ± 0.052	1.986ProGln: 1.986 ± 0.05	1.943ProArg: 1.943 ± 0.054	3.194ProSer: 3.194 ± 0.058	3.062ProThr: 3.062 ± 0.065	3.881ProVal: 3.881 ± 0.074	0.677ProTrp: 0.677 ± 0.03	1.028ProTyr: 1.028 ± 0.037	0.001ProXaa: 0.001 ± 0.001
Gln	6.673GlnAla: 6.673 ± 0.112	0.436GlnCys: 0.436 ± 0.025	1.982GlnAsp: 1.982 ± 0.046	1.624GlnGlu: 1.624 ± 0.046	1.463GlnPhe: 1.463 ± 0.04	3.187GlnGly: 3.187 ± 0.065	1.385GlnHis: 1.385 ± 0.048	1.625GlnIle: 1.625 ± 0.038	0.979GlnLys: 0.979 ± 0.035	4.889GlnLeu: 4.889 ± 0.089	0.998GlnMet: 0.998 ± 0.029	0.874GlnAsn: 0.874 ± 0.031	2.17GlnPro: 2.17 ± 0.049	2.318GlnGln: 2.318 ± 0.07	3.32GlnArg: 3.32 ± 0.069	2.374GlnSer: 2.374 ± 0.047	2.638GlnThr: 2.638 ± 0.068	3.444GlnVal: 3.444 ± 0.057	0.865GlnTrp: 0.865 ± 0.051	0.821GlnTyr: 0.821 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	5.634ArgAla: 5.634 ± 0.076	0.546ArgCys: 0.546 ± 0.026	3.136ArgAsp: 3.136 ± 0.07	2.93ArgGlu: 2.93 ± 0.067	2.285ArgPhe: 2.285 ± 0.053	3.646ArgGly: 3.646 ± 0.073	1.474ArgHis: 1.474 ± 0.042	2.987ArgIle: 2.987 ± 0.054	1.768ArgLys: 1.768 ± 0.044	6.101ArgLeu: 6.101 ± 0.098	1.768ArgMet: 1.768 ± 0.05	1.734ArgAsn: 1.734 ± 0.05	2.166ArgPro: 2.166 ± 0.046	2.151ArgGln: 2.151 ± 0.056	2.921ArgArg: 2.921 ± 0.065	3.17ArgSer: 3.17 ± 0.074	2.681ArgThr: 2.681 ± 0.058	4.494ArgVal: 4.494 ± 0.064	0.921ArgTrp: 0.921 ± 0.036	1.508ArgTyr: 1.508 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	6.827SerAla: 6.827 ± 0.099	0.564SerCys: 0.564 ± 0.024	3.293SerAsp: 3.293 ± 0.061	2.658SerGlu: 2.658 ± 0.057	2.3SerPhe: 2.3 ± 0.059	5.384SerGly: 5.384 ± 0.087	1.619SerHis: 1.619 ± 0.042	2.714SerIle: 2.714 ± 0.067	2.236SerLys: 2.236 ± 0.057	6.269SerLeu: 6.269 ± 0.1	1.815SerMet: 1.815 ± 0.042	2.092SerAsn: 2.092 ± 0.05	2.943SerPro: 2.943 ± 0.06	2.493SerGln: 2.493 ± 0.058	3.111SerArg: 3.111 ± 0.062	3.754SerSer: 3.754 ± 0.08	3.444SerThr: 3.444 ± 0.073	4.681SerVal: 4.681 ± 0.075	0.871SerTrp: 0.871 ± 0.034	1.353SerTyr: 1.353 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	6.231ThrAla: 6.231 ± 0.088	0.564ThrCys: 0.564 ± 0.027	3.014ThrAsp: 3.014 ± 0.064	2.342ThrGlu: 2.342 ± 0.053	1.901ThrPhe: 1.901 ± 0.052	4.719ThrGly: 4.719 ± 0.084	1.674ThrHis: 1.674 ± 0.05	2.479ThrIle: 2.479 ± 0.05	1.796ThrLys: 1.796 ± 0.048	6.247ThrLeu: 6.247 ± 0.09	1.466ThrMet: 1.466 ± 0.045	1.865ThrAsn: 1.865 ± 0.045	3.581ThrPro: 3.581 ± 0.064	2.629ThrGln: 2.629 ± 0.065	2.904ThrArg: 2.904 ± 0.06	3.467ThrSer: 3.467 ± 0.067	3.574ThrThr: 3.574 ± 0.066	4.557ThrVal: 4.557 ± 0.076	0.814ThrTrp: 0.814 ± 0.03	1.249ThrTyr: 1.249 ± 0.037	0.001ThrXaa: 0.001 ± 0.001
Val	9.74ValAla: 9.74 ± 0.132	0.946ValCys: 0.946 ± 0.03	4.377ValAsp: 4.377 ± 0.07	3.706ValGlu: 3.706 ± 0.072	3.123ValPhe: 3.123 ± 0.063	6.232ValGly: 6.232 ± 0.094	1.829ValHis: 1.829 ± 0.048	3.853ValIle: 3.853 ± 0.077	2.779ValLys: 2.779 ± 0.061	8.484ValLeu: 8.484 ± 0.116	2.443ValMet: 2.443 ± 0.047	2.503ValAsn: 2.503 ± 0.056	3.534ValPro: 3.534 ± 0.071	3.341ValGln: 3.341 ± 0.063	4.229ValArg: 4.229 ± 0.066	5.108ValSer: 5.108 ± 0.078	4.198ValThr: 4.198 ± 0.076	7.642ValVal: 7.642 ± 0.121	1.195ValTrp: 1.195 ± 0.04	1.677ValTyr: 1.677 ± 0.043	0.002ValXaa: 0.002 ± 0.002
Trp	1.47TrpAla: 1.47 ± 0.044	0.225TrpCys: 0.225 ± 0.018	0.677TrpAsp: 0.677 ± 0.03	0.476TrpGlu: 0.476 ± 0.028	0.578TrpPhe: 0.578 ± 0.029	1.064TrpGly: 1.064 ± 0.066	0.446TrpHis: 0.446 ± 0.022	0.604TrpIle: 0.604 ± 0.027	0.357TrpLys: 0.357 ± 0.019	2.317TrpLeu: 2.317 ± 0.062	0.508TrpMet: 0.508 ± 0.025	0.356TrpAsn: 0.356 ± 0.018	0.751TrpPro: 0.751 ± 0.034	0.9TrpGln: 0.9 ± 0.033	1.213TrpArg: 1.213 ± 0.043	0.853TrpSer: 0.853 ± 0.033	0.691TrpThr: 0.691 ± 0.029	1.217TrpVal: 1.217 ± 0.041	0.302TrpTrp: 0.302 ± 0.02	0.269TrpTyr: 0.269 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.436TyrAla: 2.436 ± 0.055	0.292TyrCys: 0.292 ± 0.016	1.137TyrAsp: 1.137 ± 0.037	1.105TyrGlu: 1.105 ± 0.034	1.06TyrPhe: 1.06 ± 0.031	1.903TyrGly: 1.903 ± 0.046	0.469TyrHis: 0.469 ± 0.022	1.035TyrIle: 1.035 ± 0.036	0.895TyrLys: 0.895 ± 0.037	2.324TyrLeu: 2.324 ± 0.066	0.593TyrMet: 0.593 ± 0.027	0.736TyrAsn: 0.736 ± 0.032	1.091TyrPro: 1.091 ± 0.033	0.984TyrGln: 0.984 ± 0.037	1.256TyrArg: 1.256 ± 0.037	1.284TyrSer: 1.284 ± 0.039	1.333TyrThr: 1.333 ± 0.038	1.712TyrVal: 1.712 ± 0.046	0.372TyrTrp: 0.372 ± 0.02	0.525TyrTyr: 0.525 ± 0.025	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.002XaaAla: 0.002 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.003XaaLeu: 0.003 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.002	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.002XaaTyr: 0.002 ± 0.002	0.043XaaXaa: 0.043 ± 0.013

Statistics based on 2683 proteins (859643 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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