Streptomyces malaysiense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6612 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J4PYF8|A0A1J4PYF8_9ACTN Nitrite reductase OS=Streptomyces malaysiense OX=1428626 GN=VT52_023770 PE=4 SV=1
MM1 pKa = 8.04DD2 pKa = 5.77IDD4 pKa = 3.9TTQPWGVAIDD14 pKa = 3.85YY15 pKa = 10.38AGRR18 pKa = 11.84ATLTEE23 pKa = 4.51AGHH26 pKa = 4.96TVYY29 pKa = 11.17VNVSDD34 pKa = 4.08TSLSSVIAPDD44 pKa = 4.51SITGTYY50 pKa = 10.56SPVTVTAQFTEE61 pKa = 4.52PGDD64 pKa = 3.49NGAVLRR70 pKa = 11.84GTGRR74 pKa = 11.84VTVMPVGTAPVVPDD88 pKa = 3.14QTAVQRR94 pKa = 11.84AVADD98 pKa = 3.81ALADD102 pKa = 4.18FTANAAAYY110 pKa = 10.17ADD112 pKa = 4.97LCAAWTPAGSGPGTGAA128 pKa = 3.59

Molecular weight: 12.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J4QAU3|A0A1J4QAU3_9ACTN DNA-binding response regulator OS=Streptomyces malaysiense OX=1428626 GN=VT52_002305 PE=4 SV=1
MM1 pKa = 7.23SAHH4 pKa = 6.69RR5 pKa = 11.84VPRR8 pKa = 11.84PNRR11 pKa = 11.84ARR13 pKa = 11.84RR14 pKa = 11.84LRR16 pKa = 11.84TALGVSALGAALAVTGTVAAQAAQSGHH43 pKa = 6.3HH44 pKa = 6.3AAGHH48 pKa = 6.86PGTAATPKK56 pKa = 9.75PVPARR61 pKa = 11.84TAAPTPVPSVPGGTATPTPVPSVPGGTATPTPVPSVPGGTATPTPVPAHH110 pKa = 6.18SASPSPVPSTPATPHH125 pKa = 6.59HH126 pKa = 6.75GPAVPAPVPAQHH138 pKa = 6.71

Molecular weight: 13.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6612

0

6612

2174823

29

6642

328.9

35.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.782 ± 0.046

0.825 ± 0.008

5.899 ± 0.025

5.687 ± 0.036

2.716 ± 0.018

9.657 ± 0.029

2.366 ± 0.013

2.983 ± 0.023

2.026 ± 0.025

10.501 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.692 ± 0.012

1.649 ± 0.018

6.175 ± 0.03

2.616 ± 0.019

8.401 ± 0.036

4.857 ± 0.028

6.105 ± 0.034

8.471 ± 0.027

1.487 ± 0.013

2.105 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski