Amino acid dipepetide frequency for Brevundimonas sp. AAP58

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.877AlaAla: 19.877 ± 0.255	1.106AlaCys: 1.106 ± 0.041	7.891AlaAsp: 7.891 ± 0.098	9.067AlaGlu: 9.067 ± 0.121	4.922AlaPhe: 4.922 ± 0.085	11.612AlaGly: 11.612 ± 0.129	2.156AlaHis: 2.156 ± 0.058	5.74AlaIle: 5.74 ± 0.083	3.201AlaLys: 3.201 ± 0.079	14.387AlaLeu: 14.387 ± 0.161	3.505AlaMet: 3.505 ± 0.069	2.842AlaAsn: 2.842 ± 0.071	6.78AlaPro: 6.78 ± 0.122	4.283AlaGln: 4.283 ± 0.082	10.838AlaArg: 10.838 ± 0.15	6.688AlaSer: 6.688 ± 0.102	6.456AlaThr: 6.456 ± 0.089	10.21AlaVal: 10.21 ± 0.122	1.985AlaTrp: 1.985 ± 0.052	2.613AlaTyr: 2.613 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	0.952CysAla: 0.952 ± 0.031	0.07CysCys: 0.07 ± 0.008	0.457CysAsp: 0.457 ± 0.023	0.377CysGlu: 0.377 ± 0.022	0.229CysPhe: 0.229 ± 0.016	0.789CysGly: 0.789 ± 0.034	0.149CysHis: 0.149 ± 0.014	0.268CysIle: 0.268 ± 0.02	0.124CysLys: 0.124 ± 0.012	0.64CysLeu: 0.64 ± 0.03	0.116CysMet: 0.116 ± 0.011	0.146CysAsn: 0.146 ± 0.014	0.408CysPro: 0.408 ± 0.024	0.217CysGln: 0.217 ± 0.015	0.507CysArg: 0.507 ± 0.024	0.357CysSer: 0.357 ± 0.021	0.327CysThr: 0.327 ± 0.023	0.598CysVal: 0.598 ± 0.026	0.098CysTrp: 0.098 ± 0.012	0.132CysTyr: 0.132 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	8.086AspAla: 8.086 ± 0.094	0.389AspCys: 0.389 ± 0.024	3.513AspAsp: 3.513 ± 0.066	3.315AspGlu: 3.315 ± 0.075	2.122AspPhe: 2.122 ± 0.057	5.964AspGly: 5.964 ± 0.1	1.339AspHis: 1.339 ± 0.038	2.686AspIle: 2.686 ± 0.063	1.248AspLys: 1.248 ± 0.049	6.34AspLeu: 6.34 ± 0.1	1.209AspMet: 1.209 ± 0.037	1.096AspAsn: 1.096 ± 0.038	4.233AspPro: 4.233 ± 0.083	2.071AspGln: 2.071 ± 0.063	5.263AspArg: 5.263 ± 0.083	1.989AspSer: 1.989 ± 0.059	2.585AspThr: 2.585 ± 0.067	4.221AspVal: 4.221 ± 0.074	1.141AspTrp: 1.141 ± 0.038	1.493AspTyr: 1.493 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	9.787GluAla: 9.787 ± 0.137	0.255GluCys: 0.255 ± 0.02	3.162GluAsp: 3.162 ± 0.067	2.958GluGlu: 2.958 ± 0.072	1.568GluPhe: 1.568 ± 0.046	5.14GluGly: 5.14 ± 0.076	1.043GluHis: 1.043 ± 0.034	2.979GluIle: 2.979 ± 0.06	1.554GluLys: 1.554 ± 0.054	4.866GluLeu: 4.866 ± 0.083	1.356GluMet: 1.356 ± 0.038	1.215GluAsn: 1.215 ± 0.039	3.205GluPro: 3.205 ± 0.067	1.991GluGln: 1.991 ± 0.051	5.556GluArg: 5.556 ± 0.097	2.132GluSer: 2.132 ± 0.049	3.949GluThr: 3.949 ± 0.074	4.312GluVal: 4.312 ± 0.086	0.744GluTrp: 0.744 ± 0.027	0.872GluTyr: 0.872 ± 0.032	0.0GluXaa: 0.0 ± 0.0
Phe	4.564PheAla: 4.564 ± 0.066	0.325PheCys: 0.325 ± 0.02	2.764PheAsp: 2.764 ± 0.059	2.297PheGlu: 2.297 ± 0.059	1.234PhePhe: 1.234 ± 0.04	3.565PheGly: 3.565 ± 0.071	0.625PheHis: 0.625 ± 0.029	1.454PheIle: 1.454 ± 0.039	0.785PheLys: 0.785 ± 0.029	3.028PheLeu: 3.028 ± 0.069	0.749PheMet: 0.749 ± 0.028	0.974PheAsn: 0.974 ± 0.037	1.441PhePro: 1.441 ± 0.042	1.058PheGln: 1.058 ± 0.038	2.307PheArg: 2.307 ± 0.057	1.826PheSer: 1.826 ± 0.049	1.965PheThr: 1.965 ± 0.055	2.824PheVal: 2.824 ± 0.06	0.538PheTrp: 0.538 ± 0.027	0.837PheTyr: 0.837 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	10.693GlyAla: 10.693 ± 0.144	0.776GlyCys: 0.776 ± 0.034	5.005GlyAsp: 5.005 ± 0.093	5.398GlyGlu: 5.398 ± 0.097	3.717GlyPhe: 3.717 ± 0.061	8.58GlyGly: 8.58 ± 0.157	1.629GlyHis: 1.629 ± 0.047	3.307GlyIle: 3.307 ± 0.07	2.379GlyLys: 2.379 ± 0.066	9.64GlyLeu: 9.64 ± 0.115	2.148GlyMet: 2.148 ± 0.065	1.678GlyAsn: 1.678 ± 0.06	4.491GlyPro: 4.491 ± 0.064	3.237GlyGln: 3.237 ± 0.07	7.358GlyArg: 7.358 ± 0.104	4.609GlySer: 4.609 ± 0.08	3.915GlyThr: 3.915 ± 0.076	7.795GlyVal: 7.795 ± 0.112	1.761GlyTrp: 1.761 ± 0.051	2.182GlyTyr: 2.182 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	2.293HisAla: 2.293 ± 0.048	0.156HisCys: 0.156 ± 0.014	1.133HisAsp: 1.133 ± 0.046	1.022HisGlu: 1.022 ± 0.032	0.608HisPhe: 0.608 ± 0.029	1.787HisGly: 1.787 ± 0.052	0.454HisHis: 0.454 ± 0.027	0.687HisIle: 0.687 ± 0.03	0.329HisLys: 0.329 ± 0.022	1.697HisLeu: 1.697 ± 0.046	0.409HisMet: 0.409 ± 0.023	0.329HisAsn: 0.329 ± 0.02	1.186HisPro: 1.186 ± 0.042	0.495HisGln: 0.495 ± 0.026	1.315HisArg: 1.315 ± 0.051	0.709HisSer: 0.709 ± 0.033	0.717HisThr: 0.717 ± 0.029	1.295HisVal: 1.295 ± 0.042	0.271HisTrp: 0.271 ± 0.017	0.443HisTyr: 0.443 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.667IleAla: 6.667 ± 0.095	0.364IleCys: 0.364 ± 0.02	3.39IleAsp: 3.39 ± 0.068	3.475IleGlu: 3.475 ± 0.058	1.317IlePhe: 1.317 ± 0.047	4.532IleGly: 4.532 ± 0.081	0.837IleHis: 0.837 ± 0.033	1.624IleIle: 1.624 ± 0.047	1.042IleLys: 1.042 ± 0.04	3.954IleLeu: 3.954 ± 0.076	0.807IleMet: 0.807 ± 0.034	1.116IleAsn: 1.116 ± 0.039	1.935IlePro: 1.935 ± 0.049	1.338IleGln: 1.338 ± 0.039	3.084IleArg: 3.084 ± 0.061	2.2IleSer: 2.2 ± 0.06	2.485IleThr: 2.485 ± 0.056	3.909IleVal: 3.909 ± 0.068	0.517IleTrp: 0.517 ± 0.023	0.799IleTyr: 0.799 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	3.917LysAla: 3.917 ± 0.083	0.088LysCys: 0.088 ± 0.011	1.353LysAsp: 1.353 ± 0.044	1.083LysGlu: 1.083 ± 0.047	0.635LysPhe: 0.635 ± 0.028	2.21LysGly: 2.21 ± 0.056	0.412LysHis: 0.412 ± 0.024	1.186LysIle: 1.186 ± 0.044	0.956LysLys: 0.956 ± 0.044	2.305LysLeu: 2.305 ± 0.066	0.463LysMet: 0.463 ± 0.025	0.54LysAsn: 0.54 ± 0.027	1.635LysPro: 1.635 ± 0.046	0.637LysGln: 0.637 ± 0.027	1.942LysArg: 1.942 ± 0.057	1.32LysSer: 1.32 ± 0.044	1.595LysThr: 1.595 ± 0.044	1.889LysVal: 1.889 ± 0.048	0.238LysTrp: 0.238 ± 0.018	0.439LysTyr: 0.439 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	13.851LeuAla: 13.851 ± 0.151	0.675LeuCys: 0.675 ± 0.029	6.348LeuAsp: 6.348 ± 0.092	5.406LeuGlu: 5.406 ± 0.084	3.512LeuPhe: 3.512 ± 0.079	8.347LeuGly: 8.347 ± 0.124	1.539LeuHis: 1.539 ± 0.047	5.284LeuIle: 5.284 ± 0.093	3.439LeuLys: 3.439 ± 0.068	8.39LeuLeu: 8.39 ± 0.128	2.362LeuMet: 2.362 ± 0.064	2.805LeuAsn: 2.805 ± 0.061	5.03LeuPro: 5.03 ± 0.087	2.441LeuGln: 2.441 ± 0.062	6.572LeuArg: 6.572 ± 0.1	6.214LeuSer: 6.214 ± 0.074	6.452LeuThr: 6.452 ± 0.088	6.84LeuVal: 6.84 ± 0.092	1.271LeuTrp: 1.271 ± 0.047	1.896LeuTyr: 1.896 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	3.284MetAla: 3.284 ± 0.062	0.105MetCys: 0.105 ± 0.012	1.133MetAsp: 1.133 ± 0.041	1.044MetGlu: 1.044 ± 0.035	0.633MetPhe: 0.633 ± 0.029	1.896MetGly: 1.896 ± 0.047	0.366MetHis: 0.366 ± 0.024	1.221MetIle: 1.221 ± 0.038	0.834MetLys: 0.834 ± 0.031	2.043MetLeu: 2.043 ± 0.053	0.565MetMet: 0.565 ± 0.03	0.634MetAsn: 0.634 ± 0.027	1.291MetPro: 1.291 ± 0.038	0.645MetGln: 0.645 ± 0.028	1.74MetArg: 1.74 ± 0.038	1.469MetSer: 1.469 ± 0.039	1.95MetThr: 1.95 ± 0.049	1.461MetVal: 1.461 ± 0.042	0.205MetTrp: 0.205 ± 0.017	0.2MetTyr: 0.2 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.051AsnAla: 3.051 ± 0.066	0.172AsnCys: 0.172 ± 0.015	1.3AsnAsp: 1.3 ± 0.046	1.004AsnGlu: 1.004 ± 0.033	0.748AsnPhe: 0.748 ± 0.036	2.275AsnGly: 2.275 ± 0.06	0.415AsnHis: 0.415 ± 0.024	1.11AsnIle: 1.11 ± 0.042	0.424AsnLys: 0.424 ± 0.025	2.421AsnLeu: 2.421 ± 0.057	0.455AsnMet: 0.455 ± 0.022	0.547AsnAsn: 0.547 ± 0.03	1.76AsnPro: 1.76 ± 0.042	0.68AsnGln: 0.68 ± 0.032	1.774AsnArg: 1.774 ± 0.046	0.991AsnSer: 0.991 ± 0.043	1.137AsnThr: 1.137 ± 0.043	1.809AsnVal: 1.809 ± 0.05	0.329AsnTrp: 0.329 ± 0.019	0.561AsnTyr: 0.561 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	7.092ProAla: 7.092 ± 0.112	0.298ProCys: 0.298 ± 0.02	4.042ProAsp: 4.042 ± 0.081	4.065ProGlu: 4.065 ± 0.073	2.055ProPhe: 2.055 ± 0.051	5.155ProGly: 5.155 ± 0.076	0.898ProHis: 0.898 ± 0.032	2.352ProIle: 2.352 ± 0.049	1.194ProLys: 1.194 ± 0.042	4.877ProLeu: 4.877 ± 0.075	1.2ProMet: 1.2 ± 0.037	1.355ProAsn: 1.355 ± 0.036	3.09ProPro: 3.09 ± 0.08	1.649ProGln: 1.649 ± 0.042	3.464ProArg: 3.464 ± 0.069	2.916ProSer: 2.916 ± 0.063	3.042ProThr: 3.042 ± 0.062	4.644ProVal: 4.644 ± 0.082	0.806ProTrp: 0.806 ± 0.033	1.095ProTyr: 1.095 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	4.83GlnAla: 4.83 ± 0.083	0.178GlnCys: 0.178 ± 0.014	1.448GlnAsp: 1.448 ± 0.043	1.309GlnGlu: 1.309 ± 0.044	0.975GlnPhe: 0.975 ± 0.033	2.734GlnGly: 2.734 ± 0.055	0.462GlnHis: 0.462 ± 0.023	1.571GlnIle: 1.571 ± 0.042	0.759GlnLys: 0.759 ± 0.03	2.59GlnLeu: 2.59 ± 0.058	0.786GlnMet: 0.786 ± 0.028	0.727GlnAsn: 0.727 ± 0.028	1.896GlnPro: 1.896 ± 0.048	0.949GlnGln: 0.949 ± 0.043	2.378GlnArg: 2.378 ± 0.052	1.741GlnSer: 1.741 ± 0.045	2.146GlnThr: 2.146 ± 0.06	2.241GlnVal: 2.241 ± 0.053	0.377GlnTrp: 0.377 ± 0.02	0.518GlnTyr: 0.518 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	9.649ArgAla: 9.649 ± 0.126	0.444ArgCys: 0.444 ± 0.025	4.577ArgAsp: 4.577 ± 0.077	4.247ArgGlu: 4.247 ± 0.081	3.076ArgPhe: 3.076 ± 0.065	5.424ArgGly: 5.424 ± 0.088	1.391ArgHis: 1.391 ± 0.043	4.045ArgIle: 4.045 ± 0.076	1.798ArgLys: 1.798 ± 0.048	9.143ArgLeu: 9.143 ± 0.117	1.964ArgMet: 1.964 ± 0.051	1.648ArgAsn: 1.648 ± 0.046	4.731ArgPro: 4.731 ± 0.089	2.521ArgGln: 2.521 ± 0.056	6.723ArgArg: 6.723 ± 0.122	3.568ArgSer: 3.568 ± 0.063	3.923ArgThr: 3.923 ± 0.065	5.409ArgVal: 5.409 ± 0.077	1.301ArgTrp: 1.301 ± 0.039	1.757ArgTyr: 1.757 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	6.49SerAla: 6.49 ± 0.092	0.292SerCys: 0.292 ± 0.019	2.974SerAsp: 2.974 ± 0.069	2.725SerGlu: 2.725 ± 0.059	1.768SerPhe: 1.768 ± 0.048	5.244SerGly: 5.244 ± 0.09	0.819SerHis: 0.819 ± 0.031	2.285SerIle: 2.285 ± 0.05	1.089SerLys: 1.089 ± 0.04	5.037SerLeu: 5.037 ± 0.085	1.036SerMet: 1.036 ± 0.032	1.266SerAsn: 1.266 ± 0.043	3.008SerPro: 3.008 ± 0.061	1.526SerGln: 1.526 ± 0.037	3.744SerArg: 3.744 ± 0.064	2.498SerSer: 2.498 ± 0.073	2.681SerThr: 2.681 ± 0.058	3.717SerVal: 3.717 ± 0.069	0.755SerTrp: 0.755 ± 0.025	1.049SerTyr: 1.049 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	7.175ThrAla: 7.175 ± 0.107	0.353ThrCys: 0.353 ± 0.023	3.162ThrAsp: 3.162 ± 0.064	2.768ThrGlu: 2.768 ± 0.055	1.931ThrPhe: 1.931 ± 0.057	5.549ThrGly: 5.549 ± 0.085	0.903ThrHis: 0.903 ± 0.036	2.323ThrIle: 2.323 ± 0.052	1.065ThrLys: 1.065 ± 0.039	6.065ThrLeu: 6.065 ± 0.091	0.969ThrMet: 0.969 ± 0.034	1.242ThrAsn: 1.242 ± 0.042	4.041ThrPro: 4.041 ± 0.075	1.508ThrGln: 1.508 ± 0.043	3.746ThrArg: 3.746 ± 0.066	2.495ThrSer: 2.495 ± 0.074	3.087ThrThr: 3.087 ± 0.069	4.727ThrVal: 4.727 ± 0.08	0.791ThrTrp: 0.791 ± 0.036	1.201ThrTyr: 1.201 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	9.774ValAla: 9.774 ± 0.121	0.63ValCys: 0.63 ± 0.028	4.465ValAsp: 4.465 ± 0.076	5.251ValGlu: 5.251 ± 0.08	2.798ValPhe: 2.798 ± 0.067	6.213ValGly: 6.213 ± 0.098	1.286ValHis: 1.286 ± 0.05	3.794ValIle: 3.794 ± 0.069	1.789ValLys: 1.789 ± 0.061	7.564ValLeu: 7.564 ± 0.111	1.833ValMet: 1.833 ± 0.048	1.881ValAsn: 1.881 ± 0.054	3.388ValPro: 3.388 ± 0.068	2.317ValGln: 2.317 ± 0.055	5.823ValArg: 5.823 ± 0.083	4.31ValSer: 4.31 ± 0.069	4.786ValThr: 4.786 ± 0.078	6.589ValVal: 6.589 ± 0.098	1.188ValTrp: 1.188 ± 0.036	1.414ValTyr: 1.414 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	1.58TrpAla: 1.58 ± 0.048	0.109TrpCys: 0.109 ± 0.014	0.758TrpAsp: 0.758 ± 0.03	0.604TrpGlu: 0.604 ± 0.026	0.565TrpPhe: 0.565 ± 0.028	1.073TrpGly: 1.073 ± 0.038	0.241TrpHis: 0.241 ± 0.017	0.762TrpIle: 0.762 ± 0.031	0.437TrpLys: 0.437 ± 0.024	1.758TrpLeu: 1.758 ± 0.046	0.411TrpMet: 0.411 ± 0.022	0.425TrpAsn: 0.425 ± 0.024	0.772TrpPro: 0.772 ± 0.033	0.441TrpGln: 0.441 ± 0.028	1.446TrpArg: 1.446 ± 0.047	0.983TrpSer: 0.983 ± 0.036	1.083TrpThr: 1.083 ± 0.036	0.935TrpVal: 0.935 ± 0.03	0.296TrpTrp: 0.296 ± 0.017	0.247TrpTyr: 0.247 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.546TyrAla: 2.546 ± 0.05	0.177TyrCys: 0.177 ± 0.016	1.426TyrAsp: 1.426 ± 0.049	1.23TyrGlu: 1.23 ± 0.039	0.746TyrPhe: 0.746 ± 0.027	2.209TyrGly: 2.209 ± 0.053	0.371TyrHis: 0.371 ± 0.022	0.714TyrIle: 0.714 ± 0.028	0.396TyrLys: 0.396 ± 0.022	1.932TyrLeu: 1.932 ± 0.045	0.375TyrMet: 0.375 ± 0.021	0.498TyrAsn: 0.498 ± 0.025	0.968TyrPro: 0.968 ± 0.035	0.614TyrGln: 0.614 ± 0.027	1.758TyrArg: 1.758 ± 0.052	0.987TyrSer: 0.987 ± 0.035	0.82TyrThr: 0.82 ± 0.032	1.668TyrVal: 1.668 ± 0.044	0.313TyrTrp: 0.313 ± 0.019	0.432TyrTyr: 0.432 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2824 proteins (853354 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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