Penguinpox virus
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 240 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A068ELR0|A0A068ELR0_9POXV DNA helicase OS=Penguinpox virus OX=648998 GN=pepv_196 PE=4 SV=1
MM1 pKa = 7.38 QYY3 pKa = 9.66 TRR5 pKa = 11.84 IIMIYY10 pKa = 9.77 IITSLLFIKK19 pKa = 9.3 VTTGITEE26 pKa = 4.24 PVKK29 pKa = 10.44 NPQDD33 pKa = 3.17 ILHH36 pKa = 6.3 IMEE39 pKa = 4.68 HH40 pKa = 6.13 NKK42 pKa = 9.63 TGVTACSLYY51 pKa = 11.04 CFDD54 pKa = 4.41 QSKK57 pKa = 10.58 GLDD60 pKa = 3.39 QPKK63 pKa = 8.87 TFILPGKK70 pKa = 9.66 YY71 pKa = 9.99 SNNSIKK77 pKa = 10.84 LEE79 pKa = 3.9 IAIDD83 pKa = 3.65 IYY85 pKa = 11.5 KK86 pKa = 10.12 KK87 pKa = 10.36 DD88 pKa = 3.62 SKK90 pKa = 10.72 PDD92 pKa = 3.47 YY93 pKa = 10.52 SHH95 pKa = 7.76 PCQAFQFCVSGNFSGKK111 pKa = 10.17 RR112 pKa = 11.84 FDD114 pKa = 3.75 HH115 pKa = 6.1 YY116 pKa = 11.25 LYY118 pKa = 10.52 GYY120 pKa = 7.99 TITGFIDD127 pKa = 3.19 IASSYY132 pKa = 9.19 YY133 pKa = 10.61 SGMSISTITLMPLQEE148 pKa = 4.61 GSLNPDD154 pKa = 3.25 SEE156 pKa = 5.15 DD157 pKa = 4.43 EE158 pKa = 4.33 EE159 pKa = 6.24 DD160 pKa = 4.12 CTTPPISTITQSQRR174 pKa = 11.84 IPEE177 pKa = 4.04 PVIKK181 pKa = 10.04 EE182 pKa = 3.72 GCRR185 pKa = 11.84 PVVLQRR191 pKa = 11.84 YY192 pKa = 9.17 AEE194 pKa = 4.38 SDD196 pKa = 3.75 DD197 pKa = 4.11 PTCIMYY203 pKa = 9.41 WDD205 pKa = 4.17 HH206 pKa = 6.67 TWDD209 pKa = 3.55 NYY211 pKa = 10.85 CDD213 pKa = 3.29 VGFFNSQQRR222 pKa = 11.84 DD223 pKa = 3.31 HH224 pKa = 7.54 DD225 pKa = 4.28 PLVFPLKK232 pKa = 10.32 SYY234 pKa = 10.87 LGIRR238 pKa = 11.84 GAFQDD243 pKa = 3.92 FQSYY247 pKa = 8.5 YY248 pKa = 10.68 CKK250 pKa = 10.68 SLDD253 pKa = 3.71 LNQSYY258 pKa = 9.43 SVCISIGEE266 pKa = 4.15 TPIAVTYY273 pKa = 10.26 HH274 pKa = 6.11 SYY276 pKa = 11.73 EE277 pKa = 4.28 NITVNEE283 pKa = 4.11 LLTRR287 pKa = 11.84 IMTLYY292 pKa = 10.96 GEE294 pKa = 4.58 EE295 pKa = 4.21 NVHH298 pKa = 6.3 KK299 pKa = 10.57 LPYY302 pKa = 10.14 RR303 pKa = 11.84 NITIMAHH310 pKa = 5.56 AQIQSLPLINGTCDD324 pKa = 3.3 TDD326 pKa = 3.88 GSNDD330 pKa = 3.89 DD331 pKa = 4.61 SDD333 pKa = 5.77 SGDD336 pKa = 5.18 DD337 pKa = 5.89 DD338 pKa = 6.88 DD339 pKa = 7.74 DD340 pKa = 7.12 DD341 pKa = 6.72 DD342 pKa = 6.75 DD343 pKa = 6.4 YY344 pKa = 12.06 YY345 pKa = 11.56 DD346 pKa = 4.8 EE347 pKa = 4.85 YY348 pKa = 11.75 DD349 pKa = 4.31 LYY351 pKa = 10.77 IEE353 pKa = 4.29 STPSIVTVKK362 pKa = 10.2 PKK364 pKa = 9.65 KK365 pKa = 9.08 TVTDD369 pKa = 4.08 EE370 pKa = 4.06 YY371 pKa = 11.72 NSIFNSFDD379 pKa = 3.22 NFDD382 pKa = 3.66 LEE384 pKa = 4.61 KK385 pKa = 10.78 RR386 pKa = 3.75
Molecular weight: 44.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.209
IPC2_protein 4.355
IPC_protein 4.355
Toseland 4.164
ProMoST 4.507
Dawson 4.342
Bjellqvist 4.495
Wikipedia 4.266
Rodwell 4.19
Grimsley 4.075
Solomon 4.342
Lehninger 4.291
Nozaki 4.444
DTASelect 4.698
Thurlkill 4.202
EMBOSS 4.279
Sillero 4.482
Patrickios 0.693
IPC_peptide 4.342
IPC2_peptide 4.469
IPC2.peptide.svr19 4.351
Protein with the highest isoelectric point:
>tr|A0A068EHK9|A0A068EHK9_9POXV Telomere-binding protein I1 OS=Penguinpox virus OX=648998 GN=pepv_093 PE=3 SV=1
MM1 pKa = 7.54 EE2 pKa = 5.37 IARR5 pKa = 11.84 EE6 pKa = 3.92 TLITIGLTILVVVLVITGFSLVLRR30 pKa = 11.84 LIPGVYY36 pKa = 9.71 SAASRR41 pKa = 11.84 SSFTAGKK48 pKa = 9.17 VLRR51 pKa = 11.84 FMEE54 pKa = 4.3 IFSTVMFIPGIIILYY69 pKa = 9.17 AAYY72 pKa = 9.46 IRR74 pKa = 11.84 KK75 pKa = 7.84 TKK77 pKa = 9.57 MKK79 pKa = 10.73 NNN81 pKa = 3.69
Molecular weight: 8.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.285
IPC2_protein 9.911
IPC_protein 10.511
Toseland 10.526
ProMoST 10.204
Dawson 10.672
Bjellqvist 10.365
Wikipedia 10.862
Rodwell 11.038
Grimsley 10.745
Solomon 10.745
Lehninger 10.716
Nozaki 10.482
DTASelect 10.365
Thurlkill 10.54
EMBOSS 10.921
Sillero 10.584
Patrickios 10.833
IPC_peptide 10.745
IPC2_peptide 9.121
IPC2.peptide.svr19 8.517
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
240
0
240
80106
34
1922
333.8
38.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.423 ± 0.104
2.177 ± 0.087
6.33 ± 0.114
5.68 ± 0.09
4.197 ± 0.109
3.902 ± 0.107
2.016 ± 0.043
10.281 ± 0.175
8.359 ± 0.151
9.2 ± 0.194
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.552 ± 0.06
7.473 ± 0.16
3.081 ± 0.106
1.909 ± 0.059
3.967 ± 0.1
7.758 ± 0.201
5.634 ± 0.116
5.661 ± 0.092
0.643 ± 0.043
5.756 ± 0.089
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here