Po-Circo-like virus 51
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G8E3Y6|G8E3Y6_9CIRC Uncharacterized protein OS=Po-Circo-like virus 51 OX=1105386 PE=4 SV=1
MM1 pKa = 7.45 YY2 pKa = 10.43 NRR4 pKa = 11.84 NYY6 pKa = 9.6 YY7 pKa = 8.97 SRR9 pKa = 11.84 RR10 pKa = 11.84 GRR12 pKa = 11.84 TIEE15 pKa = 3.92 RR16 pKa = 11.84 KK17 pKa = 7.87 KK18 pKa = 9.45 TEE20 pKa = 3.95 VSPVIRR26 pKa = 11.84 PEE28 pKa = 4.02 LVNVYY33 pKa = 10.68 YY34 pKa = 10.88 NYY36 pKa = 10.99 LKK38 pKa = 10.97 DD39 pKa = 3.46 NGLIPEE45 pKa = 4.79 PPAPIEE51 pKa = 4.58 DD52 pKa = 3.65 GSYY55 pKa = 8.33 VTYY58 pKa = 10.55 HH59 pKa = 6.7 IDD61 pKa = 3.18 QVYY64 pKa = 10.58 SFPTSITSLNSALGSLLDD82 pKa = 3.84 TFEE85 pKa = 4.42 VPVDD89 pKa = 3.24 ITEE92 pKa = 3.92 VDD94 pKa = 4.12 SIRR97 pKa = 11.84 LEE99 pKa = 4.16 YY100 pKa = 10.37 TLTPSSSSSSYY111 pKa = 10.81 RR112 pKa = 11.84 PFNMFSSSADD122 pKa = 3.18 DD123 pKa = 3.23 SGYY126 pKa = 11.28 ARR128 pKa = 11.84 YY129 pKa = 10.09 DD130 pKa = 3.44 SLPTGTLSPLKK141 pKa = 9.34 YY142 pKa = 8.92 TLFFYY147 pKa = 9.37 RR148 pKa = 11.84 TSASTFVVSIYY159 pKa = 11.07 DD160 pKa = 3.71 MKK162 pKa = 11.43 NEE164 pKa = 4.27 FYY166 pKa = 10.15 MVPTPAPLSYY176 pKa = 10.65 VSKK179 pKa = 10.85 FDD181 pKa = 3.82 DD182 pKa = 3.64 TGSTMVVKK190 pKa = 10.72 SITVSTYY197 pKa = 11.05 ANEE200 pKa = 3.91
Molecular weight: 22.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.703
IPC2_protein 4.94
IPC_protein 4.813
Toseland 4.66
ProMoST 4.825
Dawson 4.762
Bjellqvist 4.952
Wikipedia 4.647
Rodwell 4.647
Grimsley 4.571
Solomon 4.762
Lehninger 4.711
Nozaki 4.863
DTASelect 5.054
Thurlkill 4.66
EMBOSS 4.66
Sillero 4.927
Patrickios 1.265
IPC_peptide 4.762
IPC2_peptide 4.914
IPC2.peptide.svr19 4.882
Protein with the highest isoelectric point:
>tr|G8E3Y6|G8E3Y6_9CIRC Uncharacterized protein OS=Po-Circo-like virus 51 OX=1105386 PE=4 SV=1
MM1 pKa = 7.16 YY2 pKa = 10.25 GRR4 pKa = 11.84 RR5 pKa = 11.84 SYY7 pKa = 10.88 RR8 pKa = 11.84 PRR10 pKa = 11.84 RR11 pKa = 11.84 SYY13 pKa = 11.25 ARR15 pKa = 11.84 AVKK18 pKa = 9.16 PVKK21 pKa = 10.51 YY22 pKa = 10.73 SNEE25 pKa = 3.87 TFNAAFAYY33 pKa = 9.81 VWASTPTTSMVTMIPAVEE51 pKa = 4.01 SLGMRR56 pKa = 11.84 KK57 pKa = 9.24 VKK59 pKa = 10.68 NFTLSITQSPTLDD72 pKa = 3.11 SSSTVKK78 pKa = 10.45 AASSFMYY85 pKa = 10.7 ALVYY89 pKa = 10.82 LPDD92 pKa = 3.84 GVTANTLSIGNSTAASLYY110 pKa = 10.21 EE111 pKa = 4.03 PNQNVIASGVCSSNNGQFRR130 pKa = 11.84 LSSRR134 pKa = 11.84 LARR137 pKa = 11.84 NLNSGDD143 pKa = 3.46 RR144 pKa = 11.84 VVLLLRR150 pKa = 11.84 PTASQGTAGDD160 pKa = 3.77 LTNISVVLNYY170 pKa = 10.6 SITYY174 pKa = 9.4
Molecular weight: 18.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.596
IPC2_protein 9.882
IPC_protein 10.438
Toseland 9.94
ProMoST 9.911
Dawson 10.262
Bjellqvist 10.058
Wikipedia 10.526
Rodwell 10.394
Grimsley 10.394
Solomon 10.306
Lehninger 10.248
Nozaki 9.94
DTASelect 10.043
Thurlkill 10.072
EMBOSS 10.379
Sillero 10.175
Patrickios 5.041
IPC_peptide 10.292
IPC2_peptide 9.033
IPC2.peptide.svr19 8.614
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
694
174
320
231.3
26.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.764 ± 1.303
0.865 ± 0.404
5.62 ± 1.132
5.476 ± 1.446
3.458 ± 0.37
4.179 ± 0.335
1.873 ± 1.046
6.052 ± 1.189
4.179 ± 0.641
7.205 ± 0.477
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.873 ± 0.524
5.62 ± 0.608
5.331 ± 0.781
2.594 ± 0.89
6.052 ± 0.39
11.671 ± 1.795
7.349 ± 1.305
6.34 ± 1.269
1.153 ± 0.586
7.349 ± 0.785
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here