Massilia sp. KIM

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Massilia; unclassified Massilia

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4908 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S9AK23|A0A1S9AK23_9BURK Mannose-1-phosphate guanylyltransferase OS=Massilia sp. KIM OX=1955422 GN=B0920_10740 PE=3 SV=1
MM1 pKa = 7.37LCAAVVSALGALAPAQAEE19 pKa = 4.62VITFDD24 pKa = 3.23SVTNIVSGTLKK35 pKa = 10.79PGDD38 pKa = 3.44TAYY41 pKa = 9.54NTGDD45 pKa = 3.4AFTQGGYY52 pKa = 7.12QFRR55 pKa = 11.84ANNSATADD63 pKa = 3.61PSDD66 pKa = 3.95YY67 pKa = 11.01GVVGALVDD75 pKa = 3.81SDD77 pKa = 4.46NSFACLVTGCPNGTGSTYY95 pKa = 10.75YY96 pKa = 10.8AGLNDD101 pKa = 3.96GSLTVTGASPLGFTVSGLKK120 pKa = 10.04YY121 pKa = 10.75AFVAPLDD128 pKa = 3.81GLADD132 pKa = 4.3FSYY135 pKa = 11.1GRR137 pKa = 11.84LLLTGITSTGASFSTGGDD155 pKa = 3.4FAGQGANNRR164 pKa = 11.84FDD166 pKa = 3.98FADD169 pKa = 3.95FLFDD173 pKa = 4.47ASFATTAFTSLTISACLFDD192 pKa = 4.93VSGDD196 pKa = 3.91CIDD199 pKa = 3.47VHH201 pKa = 6.5EE202 pKa = 4.47LTRR205 pKa = 11.84NQAQFAIDD213 pKa = 4.21DD214 pKa = 4.2LVLNAAAAVPEE225 pKa = 4.69PGTIALLLMGMAGLGAASRR244 pKa = 11.84RR245 pKa = 11.84RR246 pKa = 11.84VLL248 pKa = 3.73

Molecular weight: 25.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S9A709|A0A1S9A709_9BURK Uncharacterized protein OS=Massilia sp. KIM OX=1955422 GN=B0920_24860 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.57RR14 pKa = 11.84THH16 pKa = 5.79GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84QVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.72RR41 pKa = 11.84LAVVV45 pKa = 3.61

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4908

0

4908

1668293

29

2788

339.9

36.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.071 ± 0.063

0.828 ± 0.011

5.261 ± 0.027

5.657 ± 0.033

3.503 ± 0.021

8.537 ± 0.039

2.153 ± 0.019

4.325 ± 0.029

3.263 ± 0.039

10.729 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.017

2.782 ± 0.026

5.262 ± 0.029

3.747 ± 0.023

7.177 ± 0.041

5.308 ± 0.029

4.819 ± 0.03

7.364 ± 0.027

1.347 ± 0.013

2.467 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski