Desulfovibrio sp. An276
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2772 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y4D062|A0A1Y4D062_9DELT 50S ribosomal protein L13 OS=Desulfovibrio sp. An276 OX=1965618 GN=rplM PE=3 SV=1
MM1 pKa = 7.68 EE2 pKa = 4.63 SVKK5 pKa = 10.9 LLGAEE10 pKa = 4.34 YY11 pKa = 10.49 NSEE14 pKa = 4.4 SYY16 pKa = 11.09 FMNSVTLFSKK26 pKa = 10.76 YY27 pKa = 9.79 PYY29 pKa = 9.69 EE30 pKa = 5.72 GEE32 pKa = 3.76 DD33 pKa = 3.68 GYY35 pKa = 11.2 RR36 pKa = 11.84 GTVIVGRR43 pKa = 11.84 DD44 pKa = 3.65 GSALFYY50 pKa = 11.07 DD51 pKa = 4.8 GNADD55 pKa = 3.7 EE56 pKa = 5.0 ASAWTSEE63 pKa = 3.99 FDD65 pKa = 3.73 PMVDD69 pKa = 2.74 NWEE72 pKa = 4.93 AIPVDD77 pKa = 3.54
Molecular weight: 8.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.795
IPC_protein 3.706
Toseland 3.516
ProMoST 3.783
Dawson 3.694
Bjellqvist 3.923
Wikipedia 3.617
Rodwell 3.541
Grimsley 3.439
Solomon 3.656
Lehninger 3.617
Nozaki 3.821
DTASelect 3.973
Thurlkill 3.579
EMBOSS 3.63
Sillero 3.821
Patrickios 0.693
IPC_peptide 3.656
IPC2_peptide 3.795
IPC2.peptide.svr19 3.804
Protein with the highest isoelectric point:
>tr|A0A1Y4DEE8|A0A1Y4DEE8_9DELT Nickel ABC transporter nickel/metallophore periplasmic binding protein OS=Desulfovibrio sp. An276 OX=1965618 GN=B5F76_02650 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.25 QPSKK9 pKa = 8.87 VRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.76 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.3 NGRR28 pKa = 11.84 AVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.15 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLSAA44 pKa = 3.86
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2772
0
2772
905338
23
4261
326.6
36.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.76 ± 0.056
1.806 ± 0.026
5.35 ± 0.041
6.406 ± 0.043
3.975 ± 0.032
7.585 ± 0.048
2.095 ± 0.023
5.23 ± 0.047
4.775 ± 0.051
10.554 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.782 ± 0.026
3.397 ± 0.038
4.66 ± 0.033
3.354 ± 0.028
6.265 ± 0.045
5.945 ± 0.034
5.188 ± 0.038
6.883 ± 0.039
1.203 ± 0.017
2.788 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here