Amino acid dipepetide frequency for Acetitomaculum ruminis DSM 5522

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.892AlaAla: 4.892 ± 0.096	0.942AlaCys: 0.942 ± 0.035	3.986AlaAsp: 3.986 ± 0.078	3.57AlaGlu: 3.57 ± 0.074	3.008AlaPhe: 3.008 ± 0.085	4.754AlaGly: 4.754 ± 0.089	0.932AlaHis: 0.932 ± 0.029	5.324AlaIle: 5.324 ± 0.089	5.548AlaLys: 5.548 ± 0.098	6.306AlaLeu: 6.306 ± 0.081	1.966AlaMet: 1.966 ± 0.05	2.96AlaAsn: 2.96 ± 0.064	1.488AlaPro: 1.488 ± 0.052	1.656AlaGln: 1.656 ± 0.051	2.259AlaArg: 2.259 ± 0.056	3.941AlaSer: 3.941 ± 0.075	3.322AlaThr: 3.322 ± 0.088	4.857AlaVal: 4.857 ± 0.089	0.411AlaTrp: 0.411 ± 0.024	2.569AlaTyr: 2.569 ± 0.069	0.0AlaXaa: 0.0 ± 0.0
Cys	0.76CysAla: 0.76 ± 0.029	0.186CysCys: 0.186 ± 0.014	0.867CysAsp: 0.867 ± 0.03	0.889CysGlu: 0.889 ± 0.036	0.609CysPhe: 0.609 ± 0.027	1.274CysGly: 1.274 ± 0.046	0.288CysHis: 0.288 ± 0.019	1.188CysIle: 1.188 ± 0.036	0.997CysLys: 0.997 ± 0.039	1.076CysLeu: 1.076 ± 0.036	0.365CysMet: 0.365 ± 0.019	0.633CysAsn: 0.633 ± 0.029	0.485CysPro: 0.485 ± 0.025	0.426CysGln: 0.426 ± 0.021	0.441CysArg: 0.441 ± 0.024	0.865CysSer: 0.865 ± 0.03	0.663CysThr: 0.663 ± 0.029	0.897CysVal: 0.897 ± 0.038	0.073CysTrp: 0.073 ± 0.01	0.537CysTyr: 0.537 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	3.928AspAla: 3.928 ± 0.081	0.796AspCys: 0.796 ± 0.028	3.624AspAsp: 3.624 ± 0.081	5.72AspGlu: 5.72 ± 0.093	3.391AspPhe: 3.391 ± 0.075	3.988AspGly: 3.988 ± 0.079	0.668AspHis: 0.668 ± 0.039	5.937AspIle: 5.937 ± 0.086	5.122AspLys: 5.122 ± 0.073	4.823AspLeu: 4.823 ± 0.08	1.802AspMet: 1.802 ± 0.045	3.314AspAsn: 3.314 ± 0.064	1.398AspPro: 1.398 ± 0.041	0.847AspGln: 0.847 ± 0.033	1.957AspArg: 1.957 ± 0.052	3.756AspSer: 3.756 ± 0.073	3.067AspThr: 3.067 ± 0.059	3.836AspVal: 3.836 ± 0.074	0.426AspTrp: 0.426 ± 0.024	3.286AspTyr: 3.286 ± 0.073	0.0AspXaa: 0.0 ± 0.0
Glu	4.865GluAla: 4.865 ± 0.075	0.795GluCys: 0.795 ± 0.03	4.63GluAsp: 4.63 ± 0.074	7.136GluGlu: 7.136 ± 0.108	3.108GluPhe: 3.108 ± 0.064	4.206GluGly: 4.206 ± 0.072	1.057GluHis: 1.057 ± 0.035	6.569GluIle: 6.569 ± 0.096	8.278GluLys: 8.278 ± 0.116	6.36GluLeu: 6.36 ± 0.09	2.114GluMet: 2.114 ± 0.054	5.542GluAsn: 5.542 ± 0.093	1.446GluPro: 1.446 ± 0.047	1.677GluGln: 1.677 ± 0.047	2.704GluArg: 2.704 ± 0.063	3.923GluSer: 3.923 ± 0.073	3.339GluThr: 3.339 ± 0.068	4.519GluVal: 4.519 ± 0.076	0.457GluTrp: 0.457 ± 0.024	3.481GluTyr: 3.481 ± 0.07	0.0GluXaa: 0.0 ± 0.0
Phe	2.605PheAla: 2.605 ± 0.054	0.65PheCys: 0.65 ± 0.025	3.04PheAsp: 3.04 ± 0.057	3.248PheGlu: 3.248 ± 0.056	2.058PhePhe: 2.058 ± 0.055	2.814PheGly: 2.814 ± 0.066	0.591PheHis: 0.591 ± 0.024	3.506PheIle: 3.506 ± 0.06	3.362PheLys: 3.362 ± 0.051	3.934PheLeu: 3.934 ± 0.089	1.164PheMet: 1.164 ± 0.037	2.266PheAsn: 2.266 ± 0.054	1.22PhePro: 1.22 ± 0.038	0.902PheGln: 0.902 ± 0.034	1.353PheArg: 1.353 ± 0.046	3.351PheSer: 3.351 ± 0.057	2.355PheThr: 2.355 ± 0.055	2.853PheVal: 2.853 ± 0.054	0.369PheTrp: 0.369 ± 0.024	1.948PheTyr: 1.948 ± 0.049	0.0PheXaa: 0.0 ± 0.0
Gly	4.099GlyAla: 4.099 ± 0.081	1.134GlyCys: 1.134 ± 0.043	3.595GlyAsp: 3.595 ± 0.061	4.391GlyGlu: 4.391 ± 0.085	3.051GlyPhe: 3.051 ± 0.059	3.959GlyGly: 3.959 ± 0.081	1.055GlyHis: 1.055 ± 0.032	6.254GlyIle: 6.254 ± 0.1	5.96GlyLys: 5.96 ± 0.091	5.211GlyLeu: 5.211 ± 0.088	1.948GlyMet: 1.948 ± 0.049	3.437GlyAsn: 3.437 ± 0.069	1.041GlyPro: 1.041 ± 0.035	1.647GlyGln: 1.647 ± 0.05	2.272GlyArg: 2.272 ± 0.054	3.842GlySer: 3.842 ± 0.073	3.234GlyThr: 3.234 ± 0.068	4.288GlyVal: 4.288 ± 0.079	0.533GlyTrp: 0.533 ± 0.025	3.117GlyTyr: 3.117 ± 0.059	0.0GlyXaa: 0.0 ± 0.0
His	0.775HisAla: 0.775 ± 0.03	0.23HisCys: 0.23 ± 0.017	0.892HisAsp: 0.892 ± 0.04	0.963HisGlu: 0.963 ± 0.037	0.723HisPhe: 0.723 ± 0.032	0.942HisGly: 0.942 ± 0.033	0.349HisHis: 0.349 ± 0.033	1.282HisIle: 1.282 ± 0.038	0.939HisLys: 0.939 ± 0.033	1.104HisLeu: 1.104 ± 0.039	0.401HisMet: 0.401 ± 0.022	0.706HisAsn: 0.706 ± 0.026	0.646HisPro: 0.646 ± 0.033	0.349HisGln: 0.349 ± 0.018	0.49HisArg: 0.49 ± 0.021	0.833HisSer: 0.833 ± 0.03	0.724HisThr: 0.724 ± 0.031	0.87HisVal: 0.87 ± 0.029	0.099HisTrp: 0.099 ± 0.011	0.633HisTyr: 0.633 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	5.547IleAla: 5.547 ± 0.1	1.292IleCys: 1.292 ± 0.04	5.241IleAsp: 5.241 ± 0.091	6.109IleGlu: 6.109 ± 0.085	3.672IlePhe: 3.672 ± 0.075	5.126IleGly: 5.126 ± 0.088	1.178IleHis: 1.178 ± 0.038	7.346IleIle: 7.346 ± 0.123	7.206IleLys: 7.206 ± 0.095	7.204IleLeu: 7.204 ± 0.122	2.095IleMet: 2.095 ± 0.051	4.832IleAsn: 4.832 ± 0.087	3.04IlePro: 3.04 ± 0.067	1.778IleGln: 1.778 ± 0.044	3.072IleArg: 3.072 ± 0.067	6.407IleSer: 6.407 ± 0.103	4.784IleThr: 4.784 ± 0.095	5.39IleVal: 5.39 ± 0.083	0.542IleTrp: 0.542 ± 0.023	3.478IleTyr: 3.478 ± 0.071	0.0IleXaa: 0.0 ± 0.0
Lys	6.076LysAla: 6.076 ± 0.1	0.883LysCys: 0.883 ± 0.032	5.828LysAsp: 5.828 ± 0.085	9.146LysGlu: 9.146 ± 0.135	2.539LysPhe: 2.539 ± 0.061	5.009LysGly: 5.009 ± 0.076	1.024LysHis: 1.024 ± 0.033	7.143LysIle: 7.143 ± 0.09	9.308LysLys: 9.308 ± 0.139	6.623LysLeu: 6.623 ± 0.097	2.487LysMet: 2.487 ± 0.052	6.107LysAsn: 6.107 ± 0.09	1.999LysPro: 1.999 ± 0.053	2.086LysGln: 2.086 ± 0.05	3.09LysArg: 3.09 ± 0.063	4.889LysSer: 4.889 ± 0.093	4.528LysThr: 4.528 ± 0.086	5.584LysVal: 5.584 ± 0.091	0.616LysTrp: 0.616 ± 0.029	3.894LysTyr: 3.894 ± 0.071	0.0LysXaa: 0.0 ± 0.0
Leu	5.525LeuAla: 5.525 ± 0.101	1.259LeuCys: 1.259 ± 0.039	5.171LeuAsp: 5.171 ± 0.085	6.482LeuGlu: 6.482 ± 0.098	3.543LeuPhe: 3.543 ± 0.071	5.271LeuGly: 5.271 ± 0.09	1.08LeuHis: 1.08 ± 0.039	6.687LeuIle: 6.687 ± 0.111	8.031LeuLys: 8.031 ± 0.106	7.568LeuLeu: 7.568 ± 0.138	2.362LeuMet: 2.362 ± 0.057	4.863LeuAsn: 4.863 ± 0.089	2.697LeuPro: 2.697 ± 0.058	1.954LeuGln: 1.954 ± 0.046	2.873LeuArg: 2.873 ± 0.068	6.856LeuSer: 6.856 ± 0.09	4.554LeuThr: 4.554 ± 0.089	5.144LeuVal: 5.144 ± 0.079	0.522LeuTrp: 0.522 ± 0.024	3.259LeuTyr: 3.259 ± 0.071	0.0LeuXaa: 0.0 ± 0.0
Met	2.184MetAla: 2.184 ± 0.056	0.322MetCys: 0.322 ± 0.018	1.766MetAsp: 1.766 ± 0.044	2.239MetGlu: 2.239 ± 0.058	1.038MetPhe: 1.038 ± 0.039	1.793MetGly: 1.793 ± 0.048	0.378MetHis: 0.378 ± 0.022	2.077MetIle: 2.077 ± 0.048	2.383MetLys: 2.383 ± 0.051	2.302MetLeu: 2.302 ± 0.058	0.758MetMet: 0.758 ± 0.031	1.471MetAsn: 1.471 ± 0.046	0.975MetPro: 0.975 ± 0.035	0.682MetGln: 0.682 ± 0.029	0.909MetArg: 0.909 ± 0.038	1.859MetSer: 1.859 ± 0.05	1.555MetThr: 1.555 ± 0.051	1.784MetVal: 1.784 ± 0.044	0.16MetTrp: 0.16 ± 0.013	0.874MetTyr: 0.874 ± 0.031	0.0MetXaa: 0.0 ± 0.0
Asn	3.509AsnAla: 3.509 ± 0.07	0.811AsnCys: 0.811 ± 0.037	3.163AsnAsp: 3.163 ± 0.074	4.168AsnGlu: 4.168 ± 0.071	2.157AsnPhe: 2.157 ± 0.054	3.723AsnGly: 3.723 ± 0.083	0.768AsnHis: 0.768 ± 0.031	5.399AsnIle: 5.399 ± 0.088	4.62AsnLys: 4.62 ± 0.087	4.716AsnLeu: 4.716 ± 0.082	1.565AsnMet: 1.565 ± 0.04	3.554AsnAsn: 3.554 ± 0.092	2.106AsnPro: 2.106 ± 0.048	1.43AsnGln: 1.43 ± 0.041	1.822AsnArg: 1.822 ± 0.046	3.592AsnSer: 3.592 ± 0.084	3.058AsnThr: 3.058 ± 0.068	3.639AsnVal: 3.639 ± 0.078	0.397AsnTrp: 0.397 ± 0.022	2.604AsnTyr: 2.604 ± 0.068	0.0AsnXaa: 0.0 ± 0.0
Pro	1.692ProAla: 1.692 ± 0.047	0.355ProCys: 0.355 ± 0.02	2.01ProAsp: 2.01 ± 0.059	2.414ProGlu: 2.414 ± 0.062	1.348ProPhe: 1.348 ± 0.04	1.728ProGly: 1.728 ± 0.048	0.46ProHis: 0.46 ± 0.025	2.02ProIle: 2.02 ± 0.048	2.078ProLys: 2.078 ± 0.049	2.315ProLeu: 2.315 ± 0.055	0.656ProMet: 0.656 ± 0.029	1.192ProAsn: 1.192 ± 0.038	0.566ProPro: 0.566 ± 0.033	0.812ProGln: 0.812 ± 0.032	0.795ProArg: 0.795 ± 0.033	1.647ProSer: 1.647 ± 0.042	1.318ProThr: 1.318 ± 0.046	2.477ProVal: 2.477 ± 0.063	0.221ProTrp: 0.221 ± 0.017	1.149ProTyr: 1.149 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	1.748GlnAla: 1.748 ± 0.049	0.257GlnCys: 0.257 ± 0.02	1.205GlnAsp: 1.205 ± 0.038	1.659GlnGlu: 1.659 ± 0.046	0.921GlnPhe: 0.921 ± 0.034	1.491GlnGly: 1.491 ± 0.04	0.328GlnHis: 0.328 ± 0.023	2.194GlnIle: 2.194 ± 0.055	2.344GlnLys: 2.344 ± 0.05	2.068GlnLeu: 2.068 ± 0.052	0.784GlnMet: 0.784 ± 0.028	1.421GlnAsn: 1.421 ± 0.048	0.565GlnPro: 0.565 ± 0.023	0.617GlnGln: 0.617 ± 0.03	0.979GlnArg: 0.979 ± 0.04	1.345GlnSer: 1.345 ± 0.04	1.242GlnThr: 1.242 ± 0.04	1.509GlnVal: 1.509 ± 0.037	0.187GlnTrp: 0.187 ± 0.013	1.022GlnTyr: 1.022 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	1.981ArgAla: 1.981 ± 0.049	0.474ArgCys: 0.474 ± 0.027	1.901ArgAsp: 1.901 ± 0.054	2.58ArgGlu: 2.58 ± 0.056	1.581ArgPhe: 1.581 ± 0.048	1.962ArgGly: 1.962 ± 0.054	0.546ArgHis: 0.546 ± 0.028	3.028ArgIle: 3.028 ± 0.058	3.142ArgLys: 3.142 ± 0.073	3.129ArgLeu: 3.129 ± 0.074	1.032ArgMet: 1.032 ± 0.035	1.915ArgAsn: 1.915 ± 0.05	0.9ArgPro: 0.9 ± 0.036	1.172ArgGln: 1.172 ± 0.04	1.379ArgArg: 1.379 ± 0.045	1.682ArgSer: 1.682 ± 0.042	1.637ArgThr: 1.637 ± 0.043	2.195ArgVal: 2.195 ± 0.049	0.23ArgTrp: 0.23 ± 0.016	1.473ArgTyr: 1.473 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	3.892SerAla: 3.892 ± 0.076	0.799SerCys: 0.799 ± 0.034	4.083SerAsp: 4.083 ± 0.083	4.122SerGlu: 4.122 ± 0.079	3.152SerPhe: 3.152 ± 0.065	4.812SerGly: 4.812 ± 0.094	0.911SerHis: 0.911 ± 0.035	5.309SerIle: 5.309 ± 0.079	5.515SerLys: 5.515 ± 0.091	5.924SerLeu: 5.924 ± 0.092	1.76SerMet: 1.76 ± 0.047	3.446SerAsn: 3.446 ± 0.067	1.618SerPro: 1.618 ± 0.041	1.905SerGln: 1.905 ± 0.048	2.148SerArg: 2.148 ± 0.045	4.219SerSer: 4.219 ± 0.095	3.12SerThr: 3.12 ± 0.068	4.319SerVal: 4.319 ± 0.082	0.471SerTrp: 0.471 ± 0.02	2.926SerTyr: 2.926 ± 0.063	0.0SerXaa: 0.0 ± 0.0
Thr	3.66ThrAla: 3.66 ± 0.09	0.599ThrCys: 0.599 ± 0.028	3.266ThrAsp: 3.266 ± 0.07	2.836ThrGlu: 2.836 ± 0.066	2.262ThrPhe: 2.262 ± 0.049	4.046ThrGly: 4.046 ± 0.073	0.726ThrHis: 0.726 ± 0.031	4.485ThrIle: 4.485 ± 0.079	4.205ThrLys: 4.205 ± 0.079	4.632ThrLeu: 4.632 ± 0.08	1.237ThrMet: 1.237 ± 0.035	2.694ThrAsn: 2.694 ± 0.062	1.735ThrPro: 1.735 ± 0.052	1.318ThrGln: 1.318 ± 0.041	1.585ThrArg: 1.585 ± 0.042	3.356ThrSer: 3.356 ± 0.085	2.857ThrThr: 2.857 ± 0.081	4.049ThrVal: 4.049 ± 0.094	0.379ThrTrp: 0.379 ± 0.018	2.21ThrTyr: 2.21 ± 0.071	0.0ThrXaa: 0.0 ± 0.0
Val	4.122ValAla: 4.122 ± 0.088	1.066ValCys: 1.066 ± 0.039	3.964ValAsp: 3.964 ± 0.066	4.692ValGlu: 4.692 ± 0.079	3.008ValPhe: 3.008 ± 0.065	3.848ValGly: 3.848 ± 0.085	0.831ValHis: 0.831 ± 0.029	5.552ValIle: 5.552 ± 0.081	5.627ValLys: 5.627 ± 0.092	6.049ValLeu: 6.049 ± 0.086	1.74ValMet: 1.74 ± 0.044	3.483ValAsn: 3.483 ± 0.071	1.864ValPro: 1.864 ± 0.046	1.236ValGln: 1.236 ± 0.034	2.162ValArg: 2.162 ± 0.054	4.854ValSer: 4.854 ± 0.087	4.135ValThr: 4.135 ± 0.085	4.516ValVal: 4.516 ± 0.092	0.412ValTrp: 0.412 ± 0.024	2.615ValTyr: 2.615 ± 0.059	0.0ValXaa: 0.0 ± 0.0
Trp	0.383TrpAla: 0.383 ± 0.021	0.098TrpCys: 0.098 ± 0.011	0.502TrpAsp: 0.502 ± 0.023	0.499TrpGlu: 0.499 ± 0.023	0.317TrpPhe: 0.317 ± 0.02	0.499TrpGly: 0.499 ± 0.023	0.144TrpHis: 0.144 ± 0.014	0.574TrpIle: 0.574 ± 0.025	0.61TrpLys: 0.61 ± 0.03	0.616TrpLeu: 0.616 ± 0.027	0.224TrpMet: 0.224 ± 0.015	0.414TrpAsn: 0.414 ± 0.026	0.154TrpPro: 0.154 ± 0.015	0.23TrpGln: 0.23 ± 0.014	0.207TrpArg: 0.207 ± 0.016	0.369TrpSer: 0.369 ± 0.022	0.335TrpThr: 0.335 ± 0.021	0.323TrpVal: 0.323 ± 0.018	0.08TrpTrp: 0.08 ± 0.009	0.295TrpTyr: 0.295 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.45TyrAla: 2.45 ± 0.057	0.574TyrCys: 0.574 ± 0.028	2.951TyrAsp: 2.951 ± 0.071	3.345TyrGlu: 3.345 ± 0.071	2.103TyrPhe: 2.103 ± 0.057	2.822TyrGly: 2.822 ± 0.058	0.635TyrHis: 0.635 ± 0.027	3.461TyrIle: 3.461 ± 0.068	3.585TyrLys: 3.585 ± 0.069	3.744TyrLeu: 3.744 ± 0.073	1.049TyrMet: 1.049 ± 0.036	2.549TyrAsn: 2.549 ± 0.056	1.307TyrPro: 1.307 ± 0.04	1.162TyrGln: 1.162 ± 0.04	1.442TyrArg: 1.442 ± 0.045	2.861TyrSer: 2.861 ± 0.064	2.311TyrThr: 2.311 ± 0.062	2.729TyrVal: 2.729 ± 0.059	0.29TyrTrp: 0.29 ± 0.02	2.396TyrTyr: 2.396 ± 0.088	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2632 proteins (888260 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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