Boseongicola aestuarii
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4011 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A238J391|A0A238J391_9RHOB Uncharacterized protein OS=Boseongicola aestuarii OX=1470561 GN=BOA8489_02554 PE=4 SV=1
MM1 pKa = 8.29 RR2 pKa = 11.84 YY3 pKa = 9.25 PILAAGLLAVTPASAQDD20 pKa = 3.81 LPVLTVYY27 pKa = 10.19 TYY29 pKa = 11.52 DD30 pKa = 4.19 SFVADD35 pKa = 4.6 WGPGPAIEE43 pKa = 4.32 TAFEE47 pKa = 4.16 ATCEE51 pKa = 4.08 CDD53 pKa = 3.53 LQFIAAGDD61 pKa = 3.85 GAALLGRR68 pKa = 11.84 VRR70 pKa = 11.84 LEE72 pKa = 4.16 GARR75 pKa = 11.84 SDD77 pKa = 3.53 ADD79 pKa = 3.13 IVLGLDD85 pKa = 3.5 TNLLAAANDD94 pKa = 3.76 TGLFAPHH101 pKa = 6.59 GLEE104 pKa = 4.42 TPALDD109 pKa = 5.8 LPVDD113 pKa = 3.4 WSDD116 pKa = 3.68 ATFLPYY122 pKa = 10.58 DD123 pKa = 3.11 WGYY126 pKa = 10.28 FAFVHH131 pKa = 5.63 NAGMEE136 pKa = 4.35 NIPTDD141 pKa = 3.9 FRR143 pKa = 11.84 GLGASDD149 pKa = 3.56 TTIVIQDD156 pKa = 3.99 PRR158 pKa = 11.84 SSTPGLGLLMWVKK171 pKa = 10.34 AAYY174 pKa = 10.0 GDD176 pKa = 3.85 EE177 pKa = 4.8 APGIWEE183 pKa = 3.99 ALADD187 pKa = 3.76 NVVTVTPGWSEE198 pKa = 4.06 AYY200 pKa = 10.34 GLFLEE205 pKa = 5.7 GEE207 pKa = 4.19 ADD209 pKa = 3.43 MVLSYY214 pKa = 7.66 TTSPAYY220 pKa = 10.24 HH221 pKa = 7.25 LIAEE225 pKa = 4.41 EE226 pKa = 4.25 DD227 pKa = 3.55 TGKK230 pKa = 8.28 TAAAFAEE237 pKa = 4.19 GHH239 pKa = 5.25 YY240 pKa = 10.42 LQVEE244 pKa = 4.63 VAGKK248 pKa = 9.47 LASSDD253 pKa = 3.72 QPEE256 pKa = 4.5 LATAFLDD263 pKa = 5.2 FMLTDD268 pKa = 4.59 AFQSVIPTTNWMYY281 pKa = 9.93 PAVMPEE287 pKa = 3.73 GGLPEE292 pKa = 4.47 GFEE295 pKa = 4.3 TLIQPDD301 pKa = 3.66 QALLLSPVAAAEE313 pKa = 4.2 ARR315 pKa = 11.84 DD316 pKa = 3.63 EE317 pKa = 5.92 ALDD320 pKa = 3.51 EE321 pKa = 4.2 WLTALSRR328 pKa = 3.8
Molecular weight: 34.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.745
IPC_protein 3.745
Toseland 3.528
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.439
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.062
Thurlkill 3.579
EMBOSS 3.668
Sillero 3.872
Patrickios 1.189
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.769
Protein with the highest isoelectric point:
>tr|A0A238J4E7|A0A238J4E7_9RHOB Tyrosine recombinase XerC OS=Boseongicola aestuarii OX=1470561 GN=xerC_3 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.81 IINARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.18 SLTAA44 pKa = 4.14
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4011
0
4011
1184372
29
2802
295.3
32.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.795 ± 0.045
0.886 ± 0.013
6.093 ± 0.04
5.961 ± 0.036
3.995 ± 0.031
8.49 ± 0.036
1.999 ± 0.02
5.566 ± 0.031
3.364 ± 0.032
9.842 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.76 ± 0.02
2.79 ± 0.02
4.865 ± 0.025
2.939 ± 0.019
6.418 ± 0.037
5.532 ± 0.029
5.595 ± 0.027
7.469 ± 0.032
1.418 ± 0.018
2.222 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here