Trinickia dinghuensis
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6498 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3D8JQC6|A0A3D8JQC6_9BURK Protein translocase subunit SecD OS=Trinickia dinghuensis OX=2291023 GN=secD PE=3 SV=1
MM1 pKa = 7.5 KK2 pKa = 10.23 LAASLSKK9 pKa = 10.8 LFAAMRR15 pKa = 11.84 ASEE18 pKa = 4.31 TSGRR22 pKa = 11.84 TQYY25 pKa = 11.54 AAVPAPLIVALEE37 pKa = 3.82 PRR39 pKa = 11.84 IVYY42 pKa = 9.28 DD43 pKa = 4.48 ASAASIGMAAAVAAQHH59 pKa = 5.67 HH60 pKa = 6.32 HH61 pKa = 6.65 ASAEE65 pKa = 4.03 AHH67 pKa = 6.68 DD68 pKa = 4.79 AATPAAVSNSAASAGRR84 pKa = 11.84 SAAFSVSSSDD94 pKa = 3.16 AAASQRR100 pKa = 11.84 AVHH103 pKa = 6.91 GYY105 pKa = 7.26 MTAQSSTGIGAGDD118 pKa = 3.99 TSTMRR123 pKa = 11.84 AVHH126 pKa = 7.03 GYY128 pKa = 8.23 TDD130 pKa = 4.25 LAVTSADD137 pKa = 3.46 KK138 pKa = 10.97 QVVFVNSNVTDD149 pKa = 3.85 YY150 pKa = 11.02 QALIAGVPQGTQVVILDD167 pKa = 3.74 STKK170 pKa = 11.11 DD171 pKa = 3.47 GLSQMEE177 pKa = 4.68 QYY179 pKa = 10.23 LQQHH183 pKa = 6.71 PGVSAIHH190 pKa = 5.92 LVSHH194 pKa = 6.63 GADD197 pKa = 3.1 GDD199 pKa = 4.1 FEE201 pKa = 5.64 IGSTWINEE209 pKa = 4.2 ADD211 pKa = 3.64 LSTYY215 pKa = 10.18 SAQLAQIGAAMKK227 pKa = 10.27 PGGDD231 pKa = 3.67 FLIYY235 pKa = 10.61 GCDD238 pKa = 3.26 VAEE241 pKa = 4.39 NADD244 pKa = 3.55 GKK246 pKa = 11.22 ALVQQIASITGLNVAASTDD265 pKa = 3.21 ITGAASLGGDD275 pKa = 3.06 WTLEE279 pKa = 3.76 YY280 pKa = 10.96 DD281 pKa = 3.84 VGNVHH286 pKa = 6.46 TNVIFSAAAEE296 pKa = 4.0 KK297 pKa = 10.81 SYY299 pKa = 11.07 DD300 pKa = 3.59 YY301 pKa = 10.92 TLALIDD307 pKa = 4.22 EE308 pKa = 5.24 NYY310 pKa = 10.17 DD311 pKa = 3.32 SHH313 pKa = 9.13 VGFDD317 pKa = 3.36 TGGTGVSSTTLDD329 pKa = 3.13 GLTYY333 pKa = 10.56 TGDD336 pKa = 3.44 QPVEE340 pKa = 4.01 YY341 pKa = 9.74 QVVSGSLPNSATGGEE356 pKa = 4.32 LVVNTQGTAMSTMTVSRR373 pKa = 11.84 ADD375 pKa = 3.49 GSLMAVQSFDD385 pKa = 3.38 IDD387 pKa = 3.19 IFLSNDD393 pKa = 3.01 VTVQAIGSSGQVLGSIDD410 pKa = 5.01 LNLNDD415 pKa = 4.16 GASTNTYY422 pKa = 10.56 DD423 pKa = 4.17 AGHH426 pKa = 6.45 SSGTSLFHH434 pKa = 7.62 VNLTSLAAFSQVKK447 pKa = 9.87 SIKK450 pKa = 10.42 FIEE453 pKa = 4.16 NSGNGTYY460 pKa = 10.89 LSPTLDD466 pKa = 3.1 NFTYY470 pKa = 10.83 LDD472 pKa = 3.77 PTSPPTLTASGGTASFTAADD492 pKa = 4.0 NAASTPVVVDD502 pKa = 3.65 PGITLTDD509 pKa = 3.91 GNSATATSATVTITGNFQAGEE530 pKa = 4.02 DD531 pKa = 3.44 QLAFINNNSTTYY543 pKa = 11.26 GNIANGSYY551 pKa = 10.34 NPSSGVLTLNGTASITQWQAALSAVTYY578 pKa = 9.57 TDD580 pKa = 3.19 TAVTPNTAMRR590 pKa = 11.84 TVSFEE595 pKa = 3.98 LTSDD599 pKa = 3.51 GFTPSNTVTRR609 pKa = 11.84 TVTVADD615 pKa = 3.82 TDD617 pKa = 3.55 QTPIVHH623 pKa = 5.5 TTGGTTSYY631 pKa = 11.76 ADD633 pKa = 3.29 GTSAVTIDD641 pKa = 3.55 SGVTVTDD648 pKa = 4.79 LDD650 pKa = 3.75 NTTQASGTVSISAGFQSGDD669 pKa = 3.5 TLSFTANSSTMGNIAIQSYY688 pKa = 10.58 NSATGVLSLTSASALATKK706 pKa = 9.74 AQWAAALSAVKK717 pKa = 10.47 FSSTSTTYY725 pKa = 11.2 GNRR728 pKa = 11.84 TISFTTNDD736 pKa = 3.44 GTQTSAAATDD746 pKa = 3.82 TVDD749 pKa = 4.15 VVNPLQLTTDD759 pKa = 3.34 SGSAAFVAGDD769 pKa = 3.84 NVTSTPVAVDD779 pKa = 3.17 SGLTLTDD786 pKa = 3.42 AATGTLSSVTVAITGNFDD804 pKa = 3.34 SSHH807 pKa = 6.98 DD808 pKa = 3.58 QLQFTNTGSITGSYY822 pKa = 10.6 SSATGVLTLTGASATLTQWQSALQSVTFEE851 pKa = 4.21 NTAVTPSNVPRR862 pKa = 11.84 TVTFTAVDD870 pKa = 3.67 SLTNTSTATRR880 pKa = 11.84 TVTVTDD886 pKa = 3.55 TDD888 pKa = 3.38 QTPIVTTTGGTTNYY902 pKa = 10.22 VGGTSGVTIDD912 pKa = 4.26 SGVGG916 pKa = 3.08
Molecular weight: 92.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.935
IPC_protein 3.986
Toseland 3.757
ProMoST 4.164
Dawson 3.999
Bjellqvist 4.139
Wikipedia 3.948
Rodwell 3.808
Grimsley 3.656
Solomon 3.986
Lehninger 3.948
Nozaki 4.101
DTASelect 4.406
Thurlkill 3.808
EMBOSS 3.961
Sillero 4.113
Patrickios 1.265
IPC_peptide 3.986
IPC2_peptide 4.088
IPC2.peptide.svr19 3.97
Protein with the highest isoelectric point:
>tr|A0A3D8K1U8|A0A3D8K1U8_9BURK Acetolactate synthase large subunit OS=Trinickia dinghuensis OX=2291023 GN=DWV00_11390 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.0 RR14 pKa = 11.84 THH16 pKa = 5.76 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 8.74 TAGGRR28 pKa = 11.84 KK29 pKa = 9.04 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.65 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LAII44 pKa = 4.0
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6498
0
6498
2122929
24
3980
326.7
35.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.839 ± 0.042
0.959 ± 0.011
5.435 ± 0.022
5.274 ± 0.034
3.674 ± 0.019
8.213 ± 0.034
2.33 ± 0.015
4.688 ± 0.022
3.027 ± 0.024
10.075 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.346 ± 0.015
2.736 ± 0.019
5.064 ± 0.024
3.48 ± 0.022
7.039 ± 0.038
5.9 ± 0.033
5.447 ± 0.032
7.691 ± 0.024
1.341 ± 0.013
2.443 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here