Streptococcus satellite phage Javan759
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZW71|A0A4D5ZW71_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan759 OX=2558854 GN=JavanS759_0020 PE=4 SV=1
MM1 pKa = 7.68 ILDD4 pKa = 4.62 LGKK7 pKa = 8.39 MTQAEE12 pKa = 4.45 FDD14 pKa = 3.72 EE15 pKa = 4.66 VMADD19 pKa = 3.21 IKK21 pKa = 11.13 ARR23 pKa = 11.84 NPNLLQLITDD33 pKa = 4.52 FLDD36 pKa = 3.62 RR37 pKa = 11.84 KK38 pKa = 8.24 VTPEE42 pKa = 4.05 EE43 pKa = 3.86 VDD45 pKa = 3.54 DD46 pKa = 4.24
Molecular weight: 5.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.494
IPC2_protein 4.113
IPC_protein 3.986
Toseland 3.783
ProMoST 4.05
Dawson 3.986
Bjellqvist 4.228
Wikipedia 3.935
Rodwell 3.821
Grimsley 3.706
Solomon 3.961
Lehninger 3.923
Nozaki 4.113
DTASelect 4.329
Thurlkill 3.859
EMBOSS 3.948
Sillero 4.113
Patrickios 4.202
IPC_peptide 3.961
IPC2_peptide 4.088
IPC2.peptide.svr19 4.054
Protein with the highest isoelectric point:
>tr|A0A4D5ZW57|A0A4D5ZW57_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan759 OX=2558854 GN=JavanS759_0018 PE=4 SV=1
MM1 pKa = 7.47 YY2 pKa = 9.76 EE3 pKa = 3.84 RR4 pKa = 11.84 FYY6 pKa = 10.83 ISSKK10 pKa = 10.22 SEE12 pKa = 3.4 SWLLSGCFLSFFCLFFRR29 pKa = 11.84 SWQPRR34 pKa = 11.84 FFRR37 pKa = 11.84 SLHH40 pKa = 5.57 INVGKK45 pKa = 10.62 SRR47 pKa = 11.84 IFKK50 pKa = 8.87 NTGKK54 pKa = 10.12 KK55 pKa = 9.47 QFSSFSEE62 pKa = 4.18 SLKK65 pKa = 10.83 SDD67 pKa = 3.09 GVNNSNPVSISSS79 pKa = 3.36
Molecular weight: 9.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.192
IPC2_protein 9.37
IPC_protein 9.428
Toseland 10.248
ProMoST 9.823
Dawson 10.379
Bjellqvist 10.028
Wikipedia 10.511
Rodwell 10.906
Grimsley 10.423
Solomon 10.423
Lehninger 10.409
Nozaki 10.277
DTASelect 9.999
Thurlkill 10.262
EMBOSS 10.628
Sillero 10.306
Patrickios 10.716
IPC_peptide 10.423
IPC2_peptide 8.873
IPC2.peptide.svr19 8.308
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
36
0
36
4743
40
521
131.8
15.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.798 ± 0.299
0.738 ± 0.148
5.714 ± 0.369
8.644 ± 0.551
4.111 ± 0.317
4.702 ± 0.341
1.434 ± 0.206
6.873 ± 0.314
9.256 ± 0.451
10.458 ± 0.373
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.467 ± 0.197
5.313 ± 0.344
2.804 ± 0.265
3.711 ± 0.256
5.376 ± 0.373
5.756 ± 0.427
6.114 ± 0.445
5.566 ± 0.396
1.012 ± 0.168
4.153 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here