Sinorhizobium phage ort11
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 103 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C2H7D4|A0A5C2H7D4_9CAUD Uncharacterized protein OS=Sinorhizobium phage ort11 OX=2599764 GN=Smphiort11_093 PE=4 SV=1
MM1 pKa = 7.4 NAQTPDD7 pKa = 3.47 EE8 pKa = 5.01 LMADD12 pKa = 3.68 YY13 pKa = 10.59 CYY15 pKa = 10.25 TSSSEE20 pKa = 4.25 TQTTSDD26 pKa = 3.2 TPYY29 pKa = 10.44 EE30 pKa = 4.13 IDD32 pKa = 4.53 LAPQEE37 pKa = 4.45 QYY39 pKa = 11.84 DD40 pKa = 3.81 FGDD43 pKa = 3.2 WATTKK48 pKa = 10.3 FSSYY52 pKa = 11.51 LEE54 pKa = 3.93 FTITNSGYY62 pKa = 11.15 ADD64 pKa = 3.48 VTISRR69 pKa = 11.84 VDD71 pKa = 3.51 LAGEE75 pKa = 4.2 FMLLEE80 pKa = 4.91 PIPNKK85 pKa = 9.78 IAAGAVYY92 pKa = 9.48 TGKK95 pKa = 10.79 LYY97 pKa = 9.88 FAPEE101 pKa = 4.23 TVGSKK106 pKa = 9.3 TGMLSVEE113 pKa = 4.4 AEE115 pKa = 3.91 NALGDD120 pKa = 4.34 KK121 pKa = 10.2 VIKK124 pKa = 10.64 LLGTAWDD131 pKa = 4.6 FLDD134 pKa = 5.43 LDD136 pKa = 3.56 IKK138 pKa = 11.21 GIVNEE143 pKa = 4.83 AGITLNDD150 pKa = 3.51 FPLWEE155 pKa = 4.43 AQLDD159 pKa = 4.41 VIVNTDD165 pKa = 3.63 LPVALVV171 pKa = 3.3
Molecular weight: 18.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.732
IPC_protein 3.706
Toseland 3.503
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.63
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 4.037
Thurlkill 3.554
EMBOSS 3.643
Sillero 3.834
Patrickios 1.85
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|A0A5C2H2W1|A0A5C2H2W1_9CAUD Uncharacterized protein OS=Sinorhizobium phage ort11 OX=2599764 GN=Smphiort11_018 PE=4 SV=1
MM1 pKa = 7.42 SKK3 pKa = 10.71 YY4 pKa = 10.29 LAGYY8 pKa = 9.47 GLLLIAIATFVAISPLKK25 pKa = 9.4 PARR28 pKa = 11.84 TYY30 pKa = 10.81 MLVQIQNDD38 pKa = 3.65 AQIVLHH44 pKa = 6.71 RR45 pKa = 11.84 NMSVTRR51 pKa = 11.84 CSLTKK56 pKa = 9.48 RR57 pKa = 11.84 QYY59 pKa = 10.3 PNSHH63 pKa = 6.36 CVQNLLTNKK72 pKa = 8.4 QARR75 pKa = 11.84 NN76 pKa = 3.38
Molecular weight: 8.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.911
IPC_protein 10.394
Toseland 10.292
ProMoST 10.043
Dawson 10.496
Bjellqvist 10.233
Wikipedia 10.701
Rodwell 10.833
Grimsley 10.584
Solomon 10.54
Lehninger 10.511
Nozaki 10.321
DTASelect 10.218
Thurlkill 10.35
EMBOSS 10.687
Sillero 10.423
Patrickios 10.599
IPC_peptide 10.54
IPC2_peptide 9.326
IPC2.peptide.svr19 8.4
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
103
0
103
23275
41
3418
226.0
25.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.905 ± 0.523
0.816 ± 0.133
6.157 ± 0.149
7.076 ± 0.259
3.936 ± 0.209
6.544 ± 0.261
1.865 ± 0.167
6.393 ± 0.221
6.389 ± 0.37
8.275 ± 0.206
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.672 ± 0.115
5.276 ± 0.165
4.082 ± 0.183
4.537 ± 0.274
4.498 ± 0.216
5.912 ± 0.239
6.436 ± 0.215
6.552 ± 0.204
1.16 ± 0.128
3.519 ± 0.257
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here